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The map label for this gene is tnsA [H]

Identifier: 126176508

GI number: 126176508

Start: 5059921

End: 5060748

Strand: Reverse

Name: tnsA [H]

Synonym: Sbal_4327

Alternate gene names: 126176508

Gene position: 5060748-5059921 (Counterclockwise)

Preceding gene: 126176509

Following gene: 126176507

Centisome position: 98.7

GC content: 38.41

Gene sequence:

>828_bases
TTGGGAAGGTTTAGGAAGCTAGAGACGCTGGAAGATTATCAGCGAGCATTAAAGAATAATTATGGCTTAGGTGAAGCTGA
TACGTACATGCCTTGGTTGCGCGTACAAGATGTTAATTCTGCCGGAAATAGCGGTAAAATTGATGGAATCAAATCTAACC
GAGTTCACCACACATTATCAGAGCAAGAATCATGCTTTTTCTACTTAGTAGAGTTTTGTGATCCTGTTATTGATATTCGT
GAGCAATTTCCCCTTCTCCCACTCGACCTTTCGGTCAGACTAGCAAAAACACTAGATGTCGATCATCCGATAAACCCAAT
GACAAAAGAAAAATGTTTCAATGTGATGACGACTGACTTTCTTTTAACTCTGACTGACGGCACTAAAACTTGGTATGAGG
CGATAAGTGTTAAGCCTGCTGATGATCTAAAAGATAAACGTACAGCTGAAAAGCTCGATATTGAGAGAGTTTGGTGGCAG
CTACAAGGTGTTGTATTTCATATATTTTCAATGACAGAGCAGAGTCAAATCCAATCTCGAAATATTCAATGGTTCACATC
TCCGTATCGGCAAGGGAGTAATTTTCAAAATGACATAATTGAAAAAGCTAAGTCTATTGTTAAATTAGGAACTAATTTAA
TTGAAGATTTATGTAATGAGTTTATTCGTGAACTGAATGTTGAGCATGATGACGCCTTAGTTTTACTGAAGGTTTTGCTA
GCGACAAAGCAAATTCGTGTGGACCTTACTAAGCCTATAGCTGAAAGTGGAATTTTGCAGGTTGTAAAGGTAGCTAGGCA
AGTTGGAGAGCAGCAAAATGCAAGTTGA

Upstream 100 bases:

>100_bases
TACATAAACAGTAAAGTTTGTACATAAGTTTATTTTGGAATAATAAAGTTTGTACACTAAGCTATAATTCACTTTATCAA
ATACTATGGGGTGAATTAGA

Downstream 100 bases:

>100_bases
ATTAAATAGCGTTTGGCTTTTTGAGGACGTTGGTGAGATTCAAGATGGAACTTACCGAGTTTTAAGTCATTACCCTGATG
ATTCAATAATTGTGATTTTC

Product: Tn7-like transposition protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MGRFRKLETLEDYQRALKNNYGLGEADTYMPWLRVQDVNSAGNSGKIDGIKSNRVHHTLSEQESCFFYLVEFCDPVIDIR
EQFPLLPLDLSVRLAKTLDVDHPINPMTKEKCFNVMTTDFLLTLTDGTKTWYEAISVKPADDLKDKRTAEKLDIERVWWQ
LQGVVFHIFSMTEQSQIQSRNIQWFTSPYRQGSNFQNDIIEKAKSIVKLGTNLIEDLCNEFIRELNVEHDDALVLLKVLL
ATKQIRVDLTKPIAESGILQVVKVARQVGEQQNAS

Sequences:

>Translated_275_residues
MGRFRKLETLEDYQRALKNNYGLGEADTYMPWLRVQDVNSAGNSGKIDGIKSNRVHHTLSEQESCFFYLVEFCDPVIDIR
EQFPLLPLDLSVRLAKTLDVDHPINPMTKEKCFNVMTTDFLLTLTDGTKTWYEAISVKPADDLKDKRTAEKLDIERVWWQ
LQGVVFHIFSMTEQSQIQSRNIQWFTSPYRQGSNFQNDIIEKAKSIVKLGTNLIEDLCNEFIRELNVEHDDALVLLKVLL
ATKQIRVDLTKPIAESGILQVVKVARQVGEQQNAS
>Mature_274_residues
GRFRKLETLEDYQRALKNNYGLGEADTYMPWLRVQDVNSAGNSGKIDGIKSNRVHHTLSEQESCFFYLVEFCDPVIDIRE
QFPLLPLDLSVRLAKTLDVDHPINPMTKEKCFNVMTTDFLLTLTDGTKTWYEAISVKPADDLKDKRTAEKLDIERVWWQL
QGVVFHIFSMTEQSQIQSRNIQWFTSPYRQGSNFQNDIIEKAKSIVKLGTNLIEDLCNEFIRELNVEHDDALVLLKVLLA
TKQIRVDLTKPIAESGILQVVKVARQVGEQQNAS

Specific function: Required for Tn7 transposition. Forms the transposase, together with TnsB. TnsA executes the 5'-DNA strand breakage reaction. TnsABC and tnsD promote high-frequency insertion of Tn7 into a specific target site known as ATT-Tn7 whereas tnsABC and tnsD prom

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011856
- InterPro:   IPR011335
- InterPro:   IPR014832
- InterPro:   IPR014833
- InterPro:   IPR011991 [H]

Pfam domain/function: PF08721 TnsA_C; PF08722 TnsA_N [H]

EC number: NA

Molecular weight: Translated: 31623; Mature: 31491

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRFRKLETLEDYQRALKNNYGLGEADTYMPWLRVQDVNSAGNSGKIDGIKSNRVHHTLS
CCCCHHHHHHHHHHHHHHCCCCCCCCHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHH
EQESCFFYLVEFCDPVIDIREQFPLLPLDLSVRLAKTLDVDHPINPMTKEKCFNVMTTDF
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHE
LLTLTDGTKTWYEAISVKPADDLKDKRTAEKLDIERVWWQLQGVVFHIFSMTEQSQIQSR
EEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NIQWFTSPYRQGSNFQNDIIEKAKSIVKLGTNLIEDLCNEFIRELNVEHDDALVLLKVLL
CCCEEECHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ATKQIRVDLTKPIAESGILQVVKVARQVGEQQNAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GRFRKLETLEDYQRALKNNYGLGEADTYMPWLRVQDVNSAGNSGKIDGIKSNRVHHTLS
CCCHHHHHHHHHHHHHHCCCCCCCCHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHH
EQESCFFYLVEFCDPVIDIREQFPLLPLDLSVRLAKTLDVDHPINPMTKEKCFNVMTTDF
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHE
LLTLTDGTKTWYEAISVKPADDLKDKRTAEKLDIERVWWQLQGVVFHIFSMTEQSQIQSR
EEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NIQWFTSPYRQGSNFQNDIIEKAKSIVKLGTNLIEDLCNEFIRELNVEHDDALVLLKVLL
CCCEEECHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ATKQIRVDLTKPIAESGILQVVKVARQVGEQQNAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2156235; 1655576; 3010949; 8947057; 10704304 [H]