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The map label for this gene is tnsB [H]

Identifier: 126176507

GI number: 126176507

Start: 5058321

End: 5059931

Strand: Reverse

Name: tnsB [H]

Synonym: Sbal_4326

Alternate gene names: 126176507

Gene position: 5059931-5058321 (Counterclockwise)

Preceding gene: 126176508

Following gene: 126176506

Centisome position: 98.68

GC content: 39.48

Gene sequence:

>1611_bases
ATGCAAGTTGAATTAAATAGCGTTTGGCTTTTTGAGGACGTTGGTGAGATTCAAGATGGAACTTACCGAGTTTTAAGTCA
TTACCCTGATGATTCAATAATTGTGATTTTCAAGCTTACGGACGATTCTAATCTCCAAAGACCTATAGCTGTCTCTATCG
CTGTATTTATTAAAGCAACAGAAGATGGCCAAGTATCCCCGTCTGATTATTTAACTCCCTTTTACCAGCTTGTTTCTGAG
GAAACGATCTCTGAAGCACATAAAAATAAGAGAAATACTCGTTTCCAACAAATTAAAGAGTTAATTACAGTTCCAAACTT
TCTTCTTGATATTTCACTTAACCCAAGATGTAAACACGTTGTGGATCACGCTAAAAAGCAAAATACATACGTTCAAGGTT
TATATCGGTCCCTCAATCTGTTTTGGAAGTATGGACAAGAGCTTAACGCATTGCTCCCAGCATACAAAAATTCTGGAGGC
ACGGGGAAGTCACGGATTGCTGGAGACGTAAAAAGAGGGTCTCCTATTCAGGTATCCACTCCAGGGCTTATTGTTCCAAA
AGGGATAAATACTAGCGAAGAGGATAAAGTTAAGTTCCTTAAAGCGATGAAGTGTTATGGCTTGAGGGGAAAGGCAGTCA
TTTATAGTCGGGTATACGATCAGATGTTAACAGAGTTTTATGCAGATGAATTAATTTCTGCAGATATTGAGCAACGTGAC
GTATGTGTTCCGAGCTATAGGGCCTTTATTTATTGGGTTAATAGATTGATCCCTAAGCAAGAGATAATTAGAAAACAGAC
ATCTTTAGGTTATTTTGATCGCAACCTCAGAGGTTTATCAGGGTCATCAACGGATCATACTGAAGTACCTGGTAGTTGTT
ATGAGTTGGATGCTACAGTTTTAGATGTACATGTAGTATCTGAAATAAGGCGTAACCATGTTTTAGGCCGACCAACACTT
TATCTTATTATAGATAAAGAAAGCCGGATGATTGTTGGTATACATGTATCGATGGAGTTTGCTTCTTGGCGTGCAGGGCG
TCAGGCTTTGGTTAATTGTGCCTCTTCTAAAAAAGAGTACTGTGCTCAATTTGGTATCAAGATCGAAGATAAAGATTGGC
CGTGTAACCATATACCACAACGACTTTTATGCGACCGAGGGGAGTTTATTTGTCAAAATGCTGAAGATTTAGCTGTTCCT
TTGATAGGACACCTTAGCATTGCCCCTCCTTATAGAGCCGACCGAAAAGGGACGATAGAGCAGCGTTTCAATATGTTGAA
TAAGCGATTAAATCACGAACTTCAGGGAACCACTCTTGGGCGACCAAGTACTCGTGGGGATCGTGATCCAAGAGCTGATG
CAATATTTACTCTAAAAGAAGTCACTACATTACTTATTGATGAGGTTTTGTCACTTAATAGGCAATACTTAGACAGCTTA
GGTGGGCAGAGCCGCTTGTTTATCGAGTCTGGTAATAAACCTTGCCCTCTCAATTATTGGAATTTTCATCTAGATAAACA
TTTACATGCCCTAAATAAGGTAAATGAGGCTGAAGTTAGAAGTCGATTATTACCTGTAGAAACGGTGTCTATTGCGCGAC
AATTACCCTGA

Upstream 100 bases:

>100_bases
CTAGCGACAAAGCAAATTCGTGTGGACCTTACTAAGCCTATAGCTGAAAGTGGAATTTTGCAGGTTGTAAAGGTAGCTAG
GCAAGTTGGAGAGCAGCAAA

Downstream 100 bases:

>100_bases
CCGGTTCAGCGACAATTAGAATGGCCGGTTGATCTGATACATTCTGGACAAAGATTCTAAGGGAGTTCTTTGTGCCAGGT
CATCGAATTACAGATCAACA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MQVELNSVWLFEDVGEIQDGTYRVLSHYPDDSIIVIFKLTDDSNLQRPIAVSIAVFIKATEDGQVSPSDYLTPFYQLVSE
ETISEAHKNKRNTRFQQIKELITVPNFLLDISLNPRCKHVVDHAKKQNTYVQGLYRSLNLFWKYGQELNALLPAYKNSGG
TGKSRIAGDVKRGSPIQVSTPGLIVPKGINTSEEDKVKFLKAMKCYGLRGKAVIYSRVYDQMLTEFYADELISADIEQRD
VCVPSYRAFIYWVNRLIPKQEIIRKQTSLGYFDRNLRGLSGSSTDHTEVPGSCYELDATVLDVHVVSEIRRNHVLGRPTL
YLIIDKESRMIVGIHVSMEFASWRAGRQALVNCASSKKEYCAQFGIKIEDKDWPCNHIPQRLLCDRGEFICQNAEDLAVP
LIGHLSIAPPYRADRKGTIEQRFNMLNKRLNHELQGTTLGRPSTRGDRDPRADAIFTLKEVTTLLIDEVLSLNRQYLDSL
GGQSRLFIESGNKPCPLNYWNFHLDKHLHALNKVNEAEVRSRLLPVETVSIARQLP

Sequences:

>Translated_536_residues
MQVELNSVWLFEDVGEIQDGTYRVLSHYPDDSIIVIFKLTDDSNLQRPIAVSIAVFIKATEDGQVSPSDYLTPFYQLVSE
ETISEAHKNKRNTRFQQIKELITVPNFLLDISLNPRCKHVVDHAKKQNTYVQGLYRSLNLFWKYGQELNALLPAYKNSGG
TGKSRIAGDVKRGSPIQVSTPGLIVPKGINTSEEDKVKFLKAMKCYGLRGKAVIYSRVYDQMLTEFYADELISADIEQRD
VCVPSYRAFIYWVNRLIPKQEIIRKQTSLGYFDRNLRGLSGSSTDHTEVPGSCYELDATVLDVHVVSEIRRNHVLGRPTL
YLIIDKESRMIVGIHVSMEFASWRAGRQALVNCASSKKEYCAQFGIKIEDKDWPCNHIPQRLLCDRGEFICQNAEDLAVP
LIGHLSIAPPYRADRKGTIEQRFNMLNKRLNHELQGTTLGRPSTRGDRDPRADAIFTLKEVTTLLIDEVLSLNRQYLDSL
GGQSRLFIESGNKPCPLNYWNFHLDKHLHALNKVNEAEVRSRLLPVETVSIARQLP
>Mature_536_residues
MQVELNSVWLFEDVGEIQDGTYRVLSHYPDDSIIVIFKLTDDSNLQRPIAVSIAVFIKATEDGQVSPSDYLTPFYQLVSE
ETISEAHKNKRNTRFQQIKELITVPNFLLDISLNPRCKHVVDHAKKQNTYVQGLYRSLNLFWKYGQELNALLPAYKNSGG
TGKSRIAGDVKRGSPIQVSTPGLIVPKGINTSEEDKVKFLKAMKCYGLRGKAVIYSRVYDQMLTEFYADELISADIEQRD
VCVPSYRAFIYWVNRLIPKQEIIRKQTSLGYFDRNLRGLSGSSTDHTEVPGSCYELDATVLDVHVVSEIRRNHVLGRPTL
YLIIDKESRMIVGIHVSMEFASWRAGRQALVNCASSKKEYCAQFGIKIEDKDWPCNHIPQRLLCDRGEFICQNAEDLAVP
LIGHLSIAPPYRADRKGTIEQRFNMLNKRLNHELQGTTLGRPSTRGDRDPRADAIFTLKEVTTLLIDEVLSLNRQYLDSL
GGQSRLFIESGNKPCPLNYWNFHLDKHLHALNKVNEAEVRSRLLPVETVSIARQLP

Specific function: Sequence-specific, DNA-binding protein required for Tn7 transposition. Recognizes sequences necessary for recombination at both left and right ends of Tn7 and, together with tnsA, forms the transposase. TnsB executes the 5'-DNA strand breakage and joining

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 60986; Mature: 60986

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVELNSVWLFEDVGEIQDGTYRVLSHYPDDSIIVIFKLTDDSNLQRPIAVSIAVFIKAT
CEEEECCEEEEHHHCCCCCCHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEEEEEEEEC
EDGQVSPSDYLTPFYQLVSEETISEAHKNKRNTRFQQIKELITVPNFLLDISLNPRCKHV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCHHHHH
VDHAKKQNTYVQGLYRSLNLFWKYGQELNALLPAYKNSGGTGKSRIAGDVKRGSPIQVST
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCEEECC
PGLIVPKGINTSEEDKVKFLKAMKCYGLRGKAVIYSRVYDQMLTEFYADELISADIEQRD
CCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VCVPSYRAFIYWVNRLIPKQEIIRKQTSLGYFDRNLRGLSGSSTDHTEVPGSCYELDATV
CCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCEEECCHHH
LDVHVVSEIRRNHVLGRPTLYLIIDKESRMIVGIHVSMEFASWRAGRQALVNCASSKKEY
HHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHH
CAQFGIKIEDKDWPCNHIPQRLLCDRGEFICQNAEDLAVPLIGHLSIAPPYRADRKGTIE
HHHCCCEEECCCCCHHHCCHHHHHCCCHHHHCCCCHHEEHHHHCEECCCCCCCCCCCCHH
QRFNMLNKRLNHELQGTTLGRPSTRGDRDPRADAIFTLKEVTTLLIDEVLSLNRQYLDSL
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHC
GGQSRLFIESGNKPCPLNYWNFHLDKHLHALNKVNEAEVRSRLLPVETVSIARQLP
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHCCC
>Mature Secondary Structure
MQVELNSVWLFEDVGEIQDGTYRVLSHYPDDSIIVIFKLTDDSNLQRPIAVSIAVFIKAT
CEEEECCEEEEHHHCCCCCCHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEEEEEEEEC
EDGQVSPSDYLTPFYQLVSEETISEAHKNKRNTRFQQIKELITVPNFLLDISLNPRCKHV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCHHHHH
VDHAKKQNTYVQGLYRSLNLFWKYGQELNALLPAYKNSGGTGKSRIAGDVKRGSPIQVST
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCEEECC
PGLIVPKGINTSEEDKVKFLKAMKCYGLRGKAVIYSRVYDQMLTEFYADELISADIEQRD
CCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VCVPSYRAFIYWVNRLIPKQEIIRKQTSLGYFDRNLRGLSGSSTDHTEVPGSCYELDATV
CCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCEEECCHHH
LDVHVVSEIRRNHVLGRPTLYLIIDKESRMIVGIHVSMEFASWRAGRQALVNCASSKKEY
HHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHH
CAQFGIKIEDKDWPCNHIPQRLLCDRGEFICQNAEDLAVPLIGHLSIAPPYRADRKGTIE
HHHCCCEEECCCCCHHHCCHHHHHCCCHHHHCCCCHHEEHHHHCEECCCCCCCCCCCCHH
QRFNMLNKRLNHELQGTTLGRPSTRGDRDPRADAIFTLKEVTTLLIDEVLSLNRQYLDSL
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHC
GGQSRLFIESGNKPCPLNYWNFHLDKHLHALNKVNEAEVRSRLLPVETVSIARQLP
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2156235; 1655576; 1657979; 8947057; 10704304 [H]