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The map label for this gene is insF1 [C]

Identifier: 126176478

GI number: 126176478

Start: 5028067

End: 5028633

Strand: Direct

Name: insF1 [C]

Synonym: Sbal_4293

Alternate gene names: 126176478

Gene position: 5028067-5028633 (Clockwise)

Preceding gene: 126176475

Following gene: 126176486

Centisome position: 98.06

GC content: 47.27

Gene sequence:

>567_bases
GTGCCACTACAATTCAGCAAGCATTGGATGAAGAAGCATCCCAACCTGCTGAAGGAAGAGGGACTACACGATGCCGAGCA
TGTACTGGTCAGCGATATTACCTATCTCGAATCAGACCAAGGCGTGCACTATTTGTCACTAGTGACCGATGCCGCGTCAC
GTAAGATAGTCGGTCATCACTTGAGTACGGACATGAAAGCAGAAAGCGTAGTGAAAGCACTGAAAATGGCGGTCAAAGAT
AAGCGCTATAGCGCTAATGCGGTCCATCACTCAGACCGAGGTTCGCAATACTGCTCAGCCGTTTATCAAGATGAACTGCA
GGCCAACCATATTCAGCCGTCGATGACAGACGGTTATGATTGCTATCAAAATGCCTTAGCAGAAAGAGTAAACGGGATCC
TGAAGCAGGAGTTTTTACTCTACCGGTGTAAAACGCTGGAAGAGCTGAAGAGACTTGTTAGAGAATCGATAGCGATATAC
AACGAAATGAGACCGCATCTGAGTTTGGATATGGAAACACCCAATCAGGTGCATAATAGAAAAGGCCAGCTACTGGAGCT
GGCCTAG

Upstream 100 bases:

>100_bases
ATTCAGCACTAAATGTTTTTCTGGTTGTCGATCTCATGTGTTCACCTAAAGTCATCTACGAGGTAATAATATCACCTCTA
TTTAGGTGGCCAAAATTAGT

Downstream 100 bases:

>100_bases
AAACTGTCAACCTATCTTAGGACGGGACAAAGATGTAGCGATTAGCAGCTCTTACCGAGACGTAATTCATAGCCCAAGAC
TTCCGCATGCACGGCGTCAC

Product: integrase catalytic subunit

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MPLQFSKHWMKKHPNLLKEEGLHDAEHVLVSDITYLESDQGVHYLSLVTDAASRKIVGHHLSTDMKAESVVKALKMAVKD
KRYSANAVHHSDRGSQYCSAVYQDELQANHIQPSMTDGYDCYQNALAERVNGILKQEFLLYRCKTLEELKRLVRESIAIY
NEMRPHLSLDMETPNQVHNRKGQLLELA

Sequences:

>Translated_188_residues
MPLQFSKHWMKKHPNLLKEEGLHDAEHVLVSDITYLESDQGVHYLSLVTDAASRKIVGHHLSTDMKAESVVKALKMAVKD
KRYSANAVHHSDRGSQYCSAVYQDELQANHIQPSMTDGYDCYQNALAERVNGILKQEFLLYRCKTLEELKRLVRESIAIY
NEMRPHLSLDMETPNQVHNRKGQLLELA
>Mature_187_residues
PLQFSKHWMKKHPNLLKEEGLHDAEHVLVSDITYLESDQGVHYLSLVTDAASRKIVGHHLSTDMKAESVVKALKMAVKDK
RYSANAVHHSDRGSQYCSAVYQDELQANHIQPSMTDGYDCYQNALAERVNGILKQEFLLYRCKTLEELKRLVRESIAIYN
EMRPHLSLDMETPNQVHNRKGQLLELA

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=134, Percent_Identity=39.5522388059701, Blast_Score=96, Evalue=2e-20,
Organism=Escherichia coli, GI1788405, Length=187, Percent_Identity=31.5508021390374, Blast_Score=93, Evalue=9e-21,
Organism=Escherichia coli, GI1787263, Length=187, Percent_Identity=31.5508021390374, Blast_Score=93, Evalue=9e-21,
Organism=Escherichia coli, GI1786753, Length=187, Percent_Identity=31.5508021390374, Blast_Score=93, Evalue=9e-21,
Organism=Escherichia coli, GI1786570, Length=187, Percent_Identity=31.5508021390374, Blast_Score=93, Evalue=9e-21,
Organism=Escherichia coli, GI1786490, Length=187, Percent_Identity=31.5508021390374, Blast_Score=93, Evalue=9e-21,
Organism=Escherichia coli, GI1789981, Length=151, Percent_Identity=33.7748344370861, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 21585; Mature: 21454

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLQFSKHWMKKHPNLLKEEGLHDAEHVLVSDITYLESDQGVHYLSLVTDAASRKIVGHH
CCCCHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
LSTDMKAESVVKALKMAVKDKRYSANAVHHSDRGSQYCSAVYQDELQANHIQPSMTDGYD
HCCCCCHHHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHH
CYQNALAERVNGILKQEFLLYRCKTLEELKRLVRESIAIYNEMRPHLSLDMETPNQVHNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHC
KGQLLELA
CCCEEECC
>Mature Secondary Structure 
PLQFSKHWMKKHPNLLKEEGLHDAEHVLVSDITYLESDQGVHYLSLVTDAASRKIVGHH
CCCHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
LSTDMKAESVVKALKMAVKDKRYSANAVHHSDRGSQYCSAVYQDELQANHIQPSMTDGYD
HCCCCCHHHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHH
CYQNALAERVNGILKQEFLLYRCKTLEELKRLVRESIAIYNEMRPHLSLDMETPNQVHNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHC
KGQLLELA
CCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]