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The map label for this gene is trmA [H]

Identifier: 126176363

GI number: 126176363

Start: 4891380

End: 4892477

Strand: Direct

Name: trmA [H]

Synonym: Sbal_4178

Alternate gene names: 126176363

Gene position: 4891380-4892477 (Clockwise)

Preceding gene: 126176361

Following gene: 126176366

Centisome position: 95.4

GC content: 44.9

Gene sequence:

>1098_bases
ATGAACTTAGCAGCAATGGACCCTCAGACTTATGATACGCAGTTAGAACACAAGCGTATCAAGCTAGAACAAGCCTTTGC
CCAATTTGAAACACCGAGTGTTGAAGTTTTTGCCTCAGAGCCTGCCAACTACCGTATGCGTGCCGAATTCCGCATGTGGC
ATGACGGTGACGACTTATACTATTACATGTTCGATAAAGTATTGAATGAGAAAGTGCGATGCGATCAATACTTGCCAGCC
AGCGTGCTCATCAATCAAATGATGTCGGCCTTAATCGCCGAACTAAAACCGAATCCTAGTCTAAGACATAAACTGTTCCA
AGTGGATTTTTTGTCGACCTTAAGCGGCGAGATCTTAGTGTCCTTGCTTTATCACAGACAGCTTGATGACCAATGGCGCG
CCGATGCGACTGCACTAAAAGCCAAGTTAAGCAGCCAGTTTAACGTCAACATCATCGGCCGTGCCCGTAAGCAAAAAATC
GATTTAGATAAAGACTTTGTGGTCGAGTCATTACAAGTGAATGATAAAACCTTCTTATATAAGCAAATCGAGAACAGCTT
TACTCAACCAAATGCCAAAGTTTCCGTAAAAATGTTGGAGTGGGCTATCGATGCGACTCAGAACAGTCAGGGTGATTTAC
TAGAACTCTACTGTGGTAATGGCAATTTCTCTATCGCACTGGCGCAAAACTTTAATCGCGTATTGGCCACCGAACTCGCT
AAGCCTTCAGTCGATGCGGCGCAATACAATATCGAAGTGAATGGTATTGAGAACCTGCAGATTATTCGCATGTCGGCCGA
AGACTTTAGCGATGCCATGGCGAAGAAACGCAGCTTTAGACGTTTAGAAGGTATCGATCTCGACAGCTATGTGTGCAACA
CCATTTTTGTCGACCCACCACGCGCAGGGATTGATCCTGATACGTTGGCGTTAGTACAAGGTTACGAGCGGATCTTATAT
ATTTCCTGCAACCCTGAAACCTTAAAAGACAATCTGCAGCAACTGAACGAAACCCATAAAGTCACTCGCTTCGCCTTGTT
TGATCAGTTCCCTTATACCGATCACATGGAAACAGGTGTACTACTGGAGCGTCGTTAG

Upstream 100 bases:

>100_bases
TTCCCACAGCTTTGCATAAACAGCAAAAGCAAGATGCTGGACATTCGTGTCACCTAGTATAATATTTGCGCCCCAATTTA
CAGCAACGGTGAATCAAACA

Downstream 100 bases:

>100_bases
TCACCTCAAATGCATTGAGCTCAACTGCATAGCAGTTAAACGCTGCACAGCACTCAAACATAAATTCGCAAAAAATAATG
GAGCCTCAGGCTTAATGCCG

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: tRNA(M-5-U54)-methyltransferase; RUMT [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MNLAAMDPQTYDTQLEHKRIKLEQAFAQFETPSVEVFASEPANYRMRAEFRMWHDGDDLYYYMFDKVLNEKVRCDQYLPA
SVLINQMMSALIAELKPNPSLRHKLFQVDFLSTLSGEILVSLLYHRQLDDQWRADATALKAKLSSQFNVNIIGRARKQKI
DLDKDFVVESLQVNDKTFLYKQIENSFTQPNAKVSVKMLEWAIDATQNSQGDLLELYCGNGNFSIALAQNFNRVLATELA
KPSVDAAQYNIEVNGIENLQIIRMSAEDFSDAMAKKRSFRRLEGIDLDSYVCNTIFVDPPRAGIDPDTLALVQGYERILY
ISCNPETLKDNLQQLNETHKVTRFALFDQFPYTDHMETGVLLERR

Sequences:

>Translated_365_residues
MNLAAMDPQTYDTQLEHKRIKLEQAFAQFETPSVEVFASEPANYRMRAEFRMWHDGDDLYYYMFDKVLNEKVRCDQYLPA
SVLINQMMSALIAELKPNPSLRHKLFQVDFLSTLSGEILVSLLYHRQLDDQWRADATALKAKLSSQFNVNIIGRARKQKI
DLDKDFVVESLQVNDKTFLYKQIENSFTQPNAKVSVKMLEWAIDATQNSQGDLLELYCGNGNFSIALAQNFNRVLATELA
KPSVDAAQYNIEVNGIENLQIIRMSAEDFSDAMAKKRSFRRLEGIDLDSYVCNTIFVDPPRAGIDPDTLALVQGYERILY
ISCNPETLKDNLQQLNETHKVTRFALFDQFPYTDHMETGVLLERR
>Mature_365_residues
MNLAAMDPQTYDTQLEHKRIKLEQAFAQFETPSVEVFASEPANYRMRAEFRMWHDGDDLYYYMFDKVLNEKVRCDQYLPA
SVLINQMMSALIAELKPNPSLRHKLFQVDFLSTLSGEILVSLLYHRQLDDQWRADATALKAKLSSQFNVNIIGRARKQKI
DLDKDFVVESLQVNDKTFLYKQIENSFTQPNAKVSVKMLEWAIDATQNSQGDLLELYCGNGNFSIALAQNFNRVLATELA
KPSVDAAQYNIEVNGIENLQIIRMSAEDFSDAMAKKRSFRRLEGIDLDSYVCNTIFVDPPRAGIDPDTLALVQGYERILY
ISCNPETLKDNLQQLNETHKVTRFALFDQFPYTDHMETGVLLERR

Specific function: Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=164, Percent_Identity=27.4390243902439, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI51173878, Length=164, Percent_Identity=27.4390243902439, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1790403, Length=355, Percent_Identity=60.8450704225352, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1789148, Length=182, Percent_Identity=31.3186813186813, Blast_Score=86, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24666579, Length=192, Percent_Identity=29.1666666666667, Blast_Score=91, Evalue=9e-19,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011869
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.35 [H]

Molecular weight: Translated: 42013; Mature: 42013

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLAAMDPQTYDTQLEHKRIKLEQAFAQFETPSVEVFASEPANYRMRAEFRMWHDGDDLY
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEEEEEECCCCEE
YYMFDKVLNEKVRCDQYLPASVLINQMMSALIAELKPNPSLRHKLFQVDFLSTLSGEILV
EHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
SLLYHRQLDDQWRADATALKAKLSSQFNVNIIGRARKQKIDLDKDFVVESLQVNDKTFLY
HHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEECCHHHCCCCCHHHHHHHHCCCCHHHHH
KQIENSFTQPNAKVSVKMLEWAIDATQNSQGDLLELYCGNGNFSIALAQNFNRVLATELA
HHHHHHCCCCCCEEEEEEEEEEHHCCCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHC
KPSVDAAQYNIEVNGIENLQIIRMSAEDFSDAMAKKRSFRRLEGIDLDSYVCNTIFVDPP
CCCCCCEEEEEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCHHEEECEEEECCC
RAGIDPDTLALVQGYERILYISCNPETLKDNLQQLNETHKVTRFALFDQFPYTDHMETGV
CCCCCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
LLERR
EEECC
>Mature Secondary Structure
MNLAAMDPQTYDTQLEHKRIKLEQAFAQFETPSVEVFASEPANYRMRAEFRMWHDGDDLY
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEEEEEECCCCEE
YYMFDKVLNEKVRCDQYLPASVLINQMMSALIAELKPNPSLRHKLFQVDFLSTLSGEILV
EHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
SLLYHRQLDDQWRADATALKAKLSSQFNVNIIGRARKQKIDLDKDFVVESLQVNDKTFLY
HHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEECCHHHCCCCCHHHHHHHHCCCCHHHHH
KQIENSFTQPNAKVSVKMLEWAIDATQNSQGDLLELYCGNGNFSIALAQNFNRVLATELA
HHHHHHCCCCCCEEEEEEEEEEHHCCCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHC
KPSVDAAQYNIEVNGIENLQIIRMSAEDFSDAMAKKRSFRRLEGIDLDSYVCNTIFVDPP
CCCCCCEEEEEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCHHEEECEEEECCC
RAGIDPDTLALVQGYERILYISCNPETLKDNLQQLNETHKVTRFALFDQFPYTDHMETGV
CCCCCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
LLERR
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA