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The map label for this gene is murI [H]

Identifier: 126176362

GI number: 126176362

Start: 4890312

End: 4891136

Strand: Reverse

Name: murI [H]

Synonym: Sbal_4177

Alternate gene names: 126176362

Gene position: 4891136-4890312 (Counterclockwise)

Preceding gene: 126176364

Following gene: 126176360

Centisome position: 95.39

GC content: 47.64

Gene sequence:

>825_bases
TTGTCGCGACCTATATTAGTGTTTGATTCTGGGATTGGTGGCCTATCCGTATTGGCTGAAATCCGCAAGAGTTTGCCCCA
TAGCGACTATTGTTATCTTTTCGACAATGCGAGGTTGCCCTATGGCGAGTTAGAAGAGCAGGTATTAATTGCTGGCTGTG
TCGCATTAGTATGTGATCTTGTGGCTCGCACCAATGCAATGATAGTCGTCGTCGCCTGCAATACCGCGAGCACTGTTGTG
CTACCGGCATTACGTGCAAACCTCAGTATTCCTGTTGTGGGCGTTGTACCCGCTATCAAACCCGCGGCACAGATGTCTAA
GAGTAAACGTATTGGACTCTTGGCCACACCAGGCACAGTTAAGCGTCATTATACCCATGAGCTCATCAGCCAATTTGCCG
ATGATTGCCATGTCGAACTTTTTGGTTGTTCTGAACTTGTCATGATGGCGGAGCAGAAAATAGCGACAGGTCAAATGGAC
ATGCACAGACTGGCAGATTTACTCGCGCCAGTCGTTGCCGCGCAATTGGACGTGCTCGTACTGGGTTGTACTCACTTCCC
GATGATCCAAGCTGAATTACAACAAGTGCTCGGTGCGGGCGTAACGCTAATGGATTCTGGCGCTGCGATAGCTAAGCGAG
TCGTGACTCTTTTAACGCAACAGAATCTTATTGTGGAGGAGCGCAGAGTAACAAATGAGAGAGAAACATTAGGGGAGTCA
GCAATGCAGGCGTTCTATACTAAAGCGGAAATCAGTGAAGGCTTAACAACAACATTAATTGATTGTGGCTTTTCAACTAT
CGAACGAATCACCACAACCAACTAA

Upstream 100 bases:

>100_bases
TTTGGTTGGATTCATTCAATCATCAAACATCGAATCAGTTAATGTTGTAATGCGTTCAATCTGTATAGCCATTTTGTTAT
AACCGAAACGGGAGACCCGC

Downstream 100 bases:

>100_bases
ATTTACAACGTCAAACAAAACAGTTTAAACGGCAATACTACTCGTCAGTACCAGTACGGTCGGCAGCTTCCGAATCTTGT
GTTTTTGCTTCTCTTACTTT

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MSRPILVFDSGIGGLSVLAEIRKSLPHSDYCYLFDNARLPYGELEEQVLIAGCVALVCDLVARTNAMIVVVACNTASTVV
LPALRANLSIPVVGVVPAIKPAAQMSKSKRIGLLATPGTVKRHYTHELISQFADDCHVELFGCSELVMMAEQKIATGQMD
MHRLADLLAPVVAAQLDVLVLGCTHFPMIQAELQQVLGAGVTLMDSGAAIAKRVVTLLTQQNLIVEERRVTNERETLGES
AMQAFYTKAEISEGLTTTLIDCGFSTIERITTTN

Sequences:

>Translated_274_residues
MSRPILVFDSGIGGLSVLAEIRKSLPHSDYCYLFDNARLPYGELEEQVLIAGCVALVCDLVARTNAMIVVVACNTASTVV
LPALRANLSIPVVGVVPAIKPAAQMSKSKRIGLLATPGTVKRHYTHELISQFADDCHVELFGCSELVMMAEQKIATGQMD
MHRLADLLAPVVAAQLDVLVLGCTHFPMIQAELQQVLGAGVTLMDSGAAIAKRVVTLLTQQNLIVEERRVTNERETLGES
AMQAFYTKAEISEGLTTTLIDCGFSTIERITTTN
>Mature_273_residues
SRPILVFDSGIGGLSVLAEIRKSLPHSDYCYLFDNARLPYGELEEQVLIAGCVALVCDLVARTNAMIVVVACNTASTVVL
PALRANLSIPVVGVVPAIKPAAQMSKSKRIGLLATPGTVKRHYTHELISQFADDCHVELFGCSELVMMAEQKIATGQMDM
HRLADLLAPVVAAQLDVLVLGCTHFPMIQAELQQVLGAGVTLMDSGAAIAKRVVTLLTQQNLIVEERRVTNERETLGESA
MQAFYTKAEISEGLTTTLIDCGFSTIERITTTN

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis [H]

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family [H]

Homologues:

Organism=Escherichia coli, GI87082355, Length=222, Percent_Identity=52.2522522522522, Blast_Score=209, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391 [H]

Pfam domain/function: PF01177 Asp_Glu_race [H]

EC number: =5.1.1.3 [H]

Molecular weight: Translated: 29543; Mature: 29412

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00923 ASP_GLU_RACEMASE_1 ; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPILVFDSGIGGLSVLAEIRKSLPHSDYCYLFDNARLPYGELEEQVLIAGCVALVCDL
CCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
VARTNAMIVVVACNTASTVVLPALRANLSIPVVGVVPAIKPAAQMSKSKRIGLLATPGTV
HHCCCCEEEEEEECCCCHHHHHHHHCCCCCCEEECCCCCCHHHHHHHCCCCEEEECCCHH
KRHYTHELISQFADDCHVELFGCSELVMMAEQKIATGQMDMHRLADLLAPVVAAQLDVLV
HHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEE
LGCTHFPMIQAELQQVLGAGVTLMDSGAAIAKRVVTLLTQQNLIVEERRVTNERETLGES
ECCCCCHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHH
AMQAFYTKAEISEGLTTTLIDCGFSTIERITTTN
HHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure 
SRPILVFDSGIGGLSVLAEIRKSLPHSDYCYLFDNARLPYGELEEQVLIAGCVALVCDL
CCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
VARTNAMIVVVACNTASTVVLPALRANLSIPVVGVVPAIKPAAQMSKSKRIGLLATPGTV
HHCCCCEEEEEEECCCCHHHHHHHHCCCCCCEEECCCCCCHHHHHHHCCCCEEEECCCHH
KRHYTHELISQFADDCHVELFGCSELVMMAEQKIATGQMDMHRLADLLAPVVAAQLDVLV
HHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEE
LGCTHFPMIQAELQQVLGAGVTLMDSGAAIAKRVVTLLTQQNLIVEERRVTNERETLGES
ECCCCCHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHH
AMQAFYTKAEISEGLTTTLIDCGFSTIERITTTN
HHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA