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The map label for this gene is birA [H]

Identifier: 126176359

GI number: 126176359

Start: 4881511

End: 4882470

Strand: Reverse

Name: birA [H]

Synonym: Sbal_4174

Alternate gene names: 126176359

Gene position: 4882470-4881511 (Counterclockwise)

Preceding gene: 126176360

Following gene: 126176357

Centisome position: 95.22

GC content: 46.98

Gene sequence:

>960_bases
ATGTCTGATAATTGGGGAAGAAAGCGCCAGATATTAGCGTTATTGTCTAGCGGTCAATTTGTTTCAGGCGAGCAGTTAGC
CACTGAACTTGGGATTTCGCGCGCCGCTGTAAATAAGCACATTGATGCGTTAGAAACCTATGGCGTAGCAATTTATAGCG
TTAAAGGTCGCGGCTATAAGCTAGCCAATCCCATCTCTTTGATTGATGCTTCACGTTTAGTGCAGTCAATTGATAACCGT
TGTTTTTATTTTGATGAGATCGCAAGCACCAACGGCTTTATGCTTAGCCATACCACTGAGCTAAAAAATGGCGATGTGTG
CGTGGCAGAGTATCAATCTGCAGGTCGCGGTCGCCGAGGTCGCACTTGGGTGTCGCCCTATGGGCATCACTTGTACTTCT
CATTGTTTTGGACATTCCCGCAGGGAATGGCACAGGCTATGGGTTTAAGTTTAGTAGTGGCGTGCACTTTGGTTGAAGTG
CTTAAATCGTTTGGGGTCGAGAATATTGGGGTTAAGTGGCCGAATGATATCTATTTGGATAACAAGAAGCTTGCTGGGAT
CTTGATTGAAATGTCGGGACAGGCGGATAGTCAGTGTCAGCTGATCATTGGTGTTGGCGTTAATATGGCGATGTCAGAGG
AGCAAGGCAAAGGTATCGATCAGCCTTGGAGTGACCTGTCAGATTTGGTCGATATGCCAGATAAGACTGCGCTTGTTATC
GAATTACAGAAGCAACTGAAGCGCGATATCCAGCTATTTGAACGTGAAGGATTAGCTGCATTCAAGGCTCGTTGGCAAGC
GGCAGACTTATTTTATGGTCGTGAAATACGTTTGCTTATGGGGGAAAAGTCTGTCGAAGGGATTTGTCGCGGTGTTGATG
AGCAGGGTGCGGTATTGCTCGAAACCGCCGACGGTGTGCAAGCATTTATCGGCGGTGAAATTAGCTTAAGGGCCCGCTAA

Upstream 100 bases:

>100_bases
CGCTTAGCACTGCATGTTATTGCGCAAGTACAGGATAAATTTGATGTTGTGCTAGAAGCGGAACCTAGAATCATGGGCGC
TAACGGTGAAGGAGATTTGT

Downstream 100 bases:

>100_bases
GCACCCAAGAGACTATTTTACAGAAAACTATTTGCGCATCAGTACGTGGGACATCAAATGATCCTGACCTTTTTGCAAGA
TCAAATGAGCTCTTTCACGG

Product: biotin--protein ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MSDNWGRKRQILALLSSGQFVSGEQLATELGISRAAVNKHIDALETYGVAIYSVKGRGYKLANPISLIDASRLVQSIDNR
CFYFDEIASTNGFMLSHTTELKNGDVCVAEYQSAGRGRRGRTWVSPYGHHLYFSLFWTFPQGMAQAMGLSLVVACTLVEV
LKSFGVENIGVKWPNDIYLDNKKLAGILIEMSGQADSQCQLIIGVGVNMAMSEEQGKGIDQPWSDLSDLVDMPDKTALVI
ELQKQLKRDIQLFEREGLAAFKARWQAADLFYGREIRLLMGEKSVEGICRGVDEQGAVLLETADGVQAFIGGEISLRAR

Sequences:

>Translated_319_residues
MSDNWGRKRQILALLSSGQFVSGEQLATELGISRAAVNKHIDALETYGVAIYSVKGRGYKLANPISLIDASRLVQSIDNR
CFYFDEIASTNGFMLSHTTELKNGDVCVAEYQSAGRGRRGRTWVSPYGHHLYFSLFWTFPQGMAQAMGLSLVVACTLVEV
LKSFGVENIGVKWPNDIYLDNKKLAGILIEMSGQADSQCQLIIGVGVNMAMSEEQGKGIDQPWSDLSDLVDMPDKTALVI
ELQKQLKRDIQLFEREGLAAFKARWQAADLFYGREIRLLMGEKSVEGICRGVDEQGAVLLETADGVQAFIGGEISLRAR
>Mature_318_residues
SDNWGRKRQILALLSSGQFVSGEQLATELGISRAAVNKHIDALETYGVAIYSVKGRGYKLANPISLIDASRLVQSIDNRC
FYFDEIASTNGFMLSHTTELKNGDVCVAEYQSAGRGRRGRTWVSPYGHHLYFSLFWTFPQGMAQAMGLSLVVACTLVEVL
KSFGVENIGVKWPNDIYLDNKKLAGILIEMSGQADSQCQLIIGVGVNMAMSEEQGKGIDQPWSDLSDLVDMPDKTALVIE
LQKQLKRDIQLFEREGLAAFKARWQAADLFYGREIRLLMGEKSVEGICRGVDEQGAVLLETADGVQAFIGGEISLRAR

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=310, Percent_Identity=45.8064516129032, Blast_Score=303, Evalue=1e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR013196
- InterPro:   IPR008988
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB; PF08279 HTH_11 [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 35197; Mature: 35066

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNWGRKRQILALLSSGQFVSGEQLATELGISRAAVNKHIDALETYGVAIYSVKGRGYK
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEE
LANPISLIDASRLVQSIDNRCFYFDEIASTNGFMLSHTTELKNGDVCVAEYQSAGRGRRG
CCCCHHHHHHHHHHHHHCCCEEEEHHHCCCCCEEEEEECCCCCCCEEEEEHHHCCCCCCC
RTWVSPYGHHLYFSLFWTFPQGMAQAMGLSLVVACTLVEVLKSFGVENIGVKWPNDIYLD
CEECCCCCCEEEEEEEHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEC
NKKLAGILIEMSGQADSQCQLIIGVGVNMAMSEEQGKGIDQPWSDLSDLVDMPDKTALVI
CCEEEEEEEEECCCCCCCEEEEEEECCCEEECHHCCCCCCCCHHHHHHHHCCCCCCEEHH
ELQKQLKRDIQLFEREGLAAFKARWQAADLFYGREIRLLMGEKSVEGICRGVDEQGAVLL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEECCEEEEEECCCHHHHHHCCCCCCCCEEE
ETADGVQAFIGGEISLRAR
EECCCCEEHHCCEEEEEEC
>Mature Secondary Structure 
SDNWGRKRQILALLSSGQFVSGEQLATELGISRAAVNKHIDALETYGVAIYSVKGRGYK
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEE
LANPISLIDASRLVQSIDNRCFYFDEIASTNGFMLSHTTELKNGDVCVAEYQSAGRGRRG
CCCCHHHHHHHHHHHHHCCCEEEEHHHCCCCCEEEEEECCCCCCCEEEEEHHHCCCCCCC
RTWVSPYGHHLYFSLFWTFPQGMAQAMGLSLVVACTLVEVLKSFGVENIGVKWPNDIYLD
CEECCCCCCEEEEEEEHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEC
NKKLAGILIEMSGQADSQCQLIIGVGVNMAMSEEQGKGIDQPWSDLSDLVDMPDKTALVI
CCEEEEEEEEECCCCCCCEEEEEEECCCEEECHHCCCCCCCCHHHHHHHHCCCCCCEEHH
ELQKQLKRDIQLFEREGLAAFKARWQAADLFYGREIRLLMGEKSVEGICRGVDEQGAVLL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEECCEEEEEECCCHHHHHHCCCCCCCCEEE
ETADGVQAFIGGEISLRAR
EECCCCEEHHCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3899863; 3536662; 8265357; 9278503; 1311302; 1328157; 1409631; 11353844 [H]