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The map label for this gene is hemB [H]

Identifier: 126176094

GI number: 126176094

Start: 4580898

End: 4581905

Strand: Direct

Name: hemB [H]

Synonym: Sbal_3904

Alternate gene names: 126176094

Gene position: 4580898-4581905 (Clockwise)

Preceding gene: 126176093

Following gene: 126176096

Centisome position: 89.34

GC content: 48.21

Gene sequence:

>1008_bases
GTGAATATCATTACCAGTGCCTTCCCTCAGCGTAGAATGCGCCGCATGCGTAAACATGATTTTAGCCGTCGTCTTATGGC
CGAAAATCAACTAACGGTCAATGACTTGATTTATCCCATGTTTGTTCTAGAGGGCACCAATCGCAGTGAAAAGATCGCCT
CTATGCCAGGTATTGAACGTTACTCAATCGATTTACTACTAAAAGAAGCCGAAGAACTGGTTGAGTTAGGCATCCCATTA
ATTGCGCTCTTCCCTGTGACGCCTGCAGAGAAAAAGACCTTAATGGCAGAAGAAGCTTATAATGCCGATGCCTTAGTGCA
GCGTGCTGTACGTGAGCTTAAAAAAGCCTTTCCACAGCTTGGCATCATGACAGACGTAGCATTGGACCCCTTCACCACTC
ATGGTCAAGACGGCATCATAGATGAGACTGGCTACATCCTAAACGACATCACCACCGAGATCTTAGTGAAGCAAGCATTG
TCACACGCCGAAGCCGGTGCAGACATAGTTGCACCGTCGGACATGATGGATGGCCGCATAGGCGCTATTCGCCAAGCCTT
AGAAGCAGCAGGCCACGTAAACACCCAAATCATGGCTTACTCAGCAAAATACTCTTCAAGCTACTACGGCCCGTTTCGTG
ACGCAGTAGGCTCAGCAGGCAATCTCAAAGGCGGCAATAAACACAGCTACCAGATGGATCCTGCAAACAGTGACGAAGCT
CTGCATGAAGTCGCACTCGATATTCAAGAAGGTGCCGACATGGTTATGGTTAAGCCAGGCATGCCGTATTTAGATATAGT
GCACCGCGTTAAAACTGAACTTGCCGTGCCAACATTCGCCTATCAAGTCAGTGGTGAATACGCCATGCACATGGCCGCCA
TCCAAAATGGTTGGTTAGCCGAAAAAGCCATCGTCATGGAATCTCTACTTTGCTTTAAGCGCGCAGGCGCAGACGGTATT
TTAACTTACTTCGCTAAACGTGCCGCACAGTGGCTTAAAGAAGCTTAG

Upstream 100 bases:

>100_bases
AGCGGATAAAGCCCTCTACCAAGCGAAAAATGGTGGACGTAATCAAGTCAGCTTATTTTCATCAGTTCAAGCACGACCAG
TACCTAATTAGGAGTCAAAT

Downstream 100 bases:

>100_bases
ACCTCGCGATAGCTGTGTATCAATTGAAGAAAGCAGCCTTATGGCTGCTTTTTTGTATCTGGTCATTGATAGCTATGGGG
ACTAAATCAGCACATTTGGT

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MNIITSAFPQRRMRRMRKHDFSRRLMAENQLTVNDLIYPMFVLEGTNRSEKIASMPGIERYSIDLLLKEAEELVELGIPL
IALFPVTPAEKKTLMAEEAYNADALVQRAVRELKKAFPQLGIMTDVALDPFTTHGQDGIIDETGYILNDITTEILVKQAL
SHAEAGADIVAPSDMMDGRIGAIRQALEAAGHVNTQIMAYSAKYSSSYYGPFRDAVGSAGNLKGGNKHSYQMDPANSDEA
LHEVALDIQEGADMVMVKPGMPYLDIVHRVKTELAVPTFAYQVSGEYAMHMAAIQNGWLAEKAIVMESLLCFKRAGADGI
LTYFAKRAAQWLKEA

Sequences:

>Translated_335_residues
MNIITSAFPQRRMRRMRKHDFSRRLMAENQLTVNDLIYPMFVLEGTNRSEKIASMPGIERYSIDLLLKEAEELVELGIPL
IALFPVTPAEKKTLMAEEAYNADALVQRAVRELKKAFPQLGIMTDVALDPFTTHGQDGIIDETGYILNDITTEILVKQAL
SHAEAGADIVAPSDMMDGRIGAIRQALEAAGHVNTQIMAYSAKYSSSYYGPFRDAVGSAGNLKGGNKHSYQMDPANSDEA
LHEVALDIQEGADMVMVKPGMPYLDIVHRVKTELAVPTFAYQVSGEYAMHMAAIQNGWLAEKAIVMESLLCFKRAGADGI
LTYFAKRAAQWLKEA
>Mature_335_residues
MNIITSAFPQRRMRRMRKHDFSRRLMAENQLTVNDLIYPMFVLEGTNRSEKIASMPGIERYSIDLLLKEAEELVELGIPL
IALFPVTPAEKKTLMAEEAYNADALVQRAVRELKKAFPQLGIMTDVALDPFTTHGQDGIIDETGYILNDITTEILVKQAL
SHAEAGADIVAPSDMMDGRIGAIRQALEAAGHVNTQIMAYSAKYSSSYYGPFRDAVGSAGNLKGGNKHSYQMDPANSDEA
LHEVALDIQEGADMVMVKPGMPYLDIVHRVKTELAVPTFAYQVSGEYAMHMAAIQNGWLAEKAIVMESLLCFKRAGADGI
LTYFAKRAAQWLKEA

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=310, Percent_Identity=42.258064516129, Blast_Score=241, Evalue=9e-64,
Organism=Escherichia coli, GI87081728, Length=315, Percent_Identity=44.7619047619048, Blast_Score=242, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6321398, Length=322, Percent_Identity=37.888198757764, Blast_Score=197, Evalue=3e-51,
Organism=Drosophila melanogaster, GI21358291, Length=317, Percent_Identity=39.7476340694006, Blast_Score=223, Evalue=1e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 37048; Mature: 37048

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIITSAFPQRRMRRMRKHDFSRRLMAENQLTVNDLIYPMFVLEGTNRSEKIASMPGIER
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEECCCCHHHHHCCCCCCH
YSIDLLLKEAEELVELGIPLIALFPVTPAEKKTLMAEEAYNADALVQRAVRELKKAFPQL
HHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
GIMTDVALDPFTTHGQDGIIDETGYILNDITTEILVKQALSHAEAGADIVAPSDMMDGRI
CCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHH
GAIRQALEAAGHVNTQIMAYSAKYSSSYYGPFRDAVGSAGNLKGGNKHSYQMDPANSDEA
HHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCEECCCCCCHHH
LHEVALDIQEGADMVMVKPGMPYLDIVHRVKTELAVPTFAYQVSGEYAMHMAAIQNGWLA
HHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCHHEEEECCHHHHHHHHHHCCCHH
EKAIVMESLLCFKRAGADGILTYFAKRAAQWLKEA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIITSAFPQRRMRRMRKHDFSRRLMAENQLTVNDLIYPMFVLEGTNRSEKIASMPGIER
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEECCCCHHHHHCCCCCCH
YSIDLLLKEAEELVELGIPLIALFPVTPAEKKTLMAEEAYNADALVQRAVRELKKAFPQL
HHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
GIMTDVALDPFTTHGQDGIIDETGYILNDITTEILVKQALSHAEAGADIVAPSDMMDGRI
CCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHH
GAIRQALEAAGHVNTQIMAYSAKYSSSYYGPFRDAVGSAGNLKGGNKHSYQMDPANSDEA
HHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCEECCCCCCHHH
LHEVALDIQEGADMVMVKPGMPYLDIVHRVKTELAVPTFAYQVSGEYAMHMAAIQNGWLA
HHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCHHEEEECCHHHHHHHHHHCCCHH
EKAIVMESLLCFKRAGADGILTYFAKRAAQWLKEA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9529530; 10984043; 10356331; 12079382 [H]