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The map label for this gene is maeB [H]

Identifier: 126176032

GI number: 126176032

Start: 4513809

End: 4515053

Strand: Reverse

Name: maeB [H]

Synonym: Sbal_3841

Alternate gene names: 126176032

Gene position: 4515053-4513809 (Counterclockwise)

Preceding gene: 126176033

Following gene: 126176031

Centisome position: 88.06

GC content: 46.43

Gene sequence:

>1245_bases
ATGTCAGATATTCGTCAACAAGCACTCGATTATCATGAATTTCCAATACCAGGCAAAACCGCAGTTTGCCTTACCAAACC
TGCCGAAACGAGTCACGATCTCGCCCTAGCTTATAGCCCTGGTGTGGCTGAGCCTGTGCGTGAAATCGCGGCTAATCCTG
ACAATGCGTATCGTTATACCAATAAAGGTAATACGGTTGCCGTGATTACTAATGGTACGGCGATCTTAGGTTTAGGCAAT
TTAGGCCCCTTGGCTTCTAAACCTGTGATGGAAGGCAAAGCATTATTATTCAAGCATTTTGCAAATATCGATTCGACTGA
TATTGAGATCACCCACAATACGCCTGAAGAATTTATCAATACAGTTGCAGCGATTGCTGACACTTTTGGTGGTATCAATC
TTGAAGATATTAAAGCGCCCGAATGCTTTGTTATCGAACGTGAATTGATTGCCCGTTGTAAAGTGCCGGTATTCCATGAC
GATCAGCACGGCACCGCAATTGTGACTGCGGCAGGTATGATTAATGCGCTGGAAATTCAAGGCAAGTTGATCAGCGACGC
AACGTTTGTGTGTATGGGCGCCGGCGCTGCTGCCATTGCTTGTATGACTATGCTGGTCAAATGTGGTGCGAATCGCGCTA
ACATTTATATGCTAGACACTAAAGGTGTTATTCATTCTGGCCGTACGGATCTTAATGAATATAAAGCGCTATTTGCGAAT
GATACCGATAAACGCACGCTTCTTGATGTTATTAAAGGCGCAGACGTGTTTTTAGGCCTATCAGGTCCTGACGTGATTGG
TGCAGAAGAAGTTGCGCTGATGGCTGATAAGCCGGTGATTTTTGCTTGTTCTAACCCAGATCCTGAAATCAAACCGGCAC
TGGCTCACGCAACGCGTAGCGATTTGATCATGGGTACCGGTCGCAGCGATTATCCAAACCAAGTGAATAACGTATTGTGT
TTCCCGTTTATTTTCCGTGGTGCCTTGGATGTGCGTGCGTCATGCATTAATGATGAGATGAAAATCGCCGCGGTTCATGC
CATTGCTGCACTGGCAAAAGAGCCTGTGCCTGCGAGTGTATTAAAGGCTTATCCTAATGTGACTTCACTGAGTTTTGGCC
CCGAATACGTGCTGCCAAAACCTATGGACCCACGTTTATTGCCTAATGTTGCCCGTGCGGTTGCACAGGCGGCTATCGAC
TCAGGTGTTGCTGCTATCCCTACTTTACCTAAATACGCCGACTAG

Upstream 100 bases:

>100_bases
CGACCAGCCAGACTATTAACATCACCCTTTGGTGAGACTAGATGTGTGTTGGTAGGCCTTACTCTCATAAATTATAAATG
TCGCATTTCAGGATTTTAAA

Downstream 100 bases:

>100_bases
GCTAAAAGTGGTAAAAACATCGAAAAGAGGCTAATTAGCCTCTTTTTTGTTTTTAAGTGGACATTAAAGTCACAGCGTCT
TGGTTGCCTTTTAGGTTATG

Product: malate dehydrogenase

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MSDIRQQALDYHEFPIPGKTAVCLTKPAETSHDLALAYSPGVAEPVREIAANPDNAYRYTNKGNTVAVITNGTAILGLGN
LGPLASKPVMEGKALLFKHFANIDSTDIEITHNTPEEFINTVAAIADTFGGINLEDIKAPECFVIERELIARCKVPVFHD
DQHGTAIVTAAGMINALEIQGKLISDATFVCMGAGAAAIACMTMLVKCGANRANIYMLDTKGVIHSGRTDLNEYKALFAN
DTDKRTLLDVIKGADVFLGLSGPDVIGAEEVALMADKPVIFACSNPDPEIKPALAHATRSDLIMGTGRSDYPNQVNNVLC
FPFIFRGALDVRASCINDEMKIAAVHAIAALAKEPVPASVLKAYPNVTSLSFGPEYVLPKPMDPRLLPNVARAVAQAAID
SGVAAIPTLPKYAD

Sequences:

>Translated_414_residues
MSDIRQQALDYHEFPIPGKTAVCLTKPAETSHDLALAYSPGVAEPVREIAANPDNAYRYTNKGNTVAVITNGTAILGLGN
LGPLASKPVMEGKALLFKHFANIDSTDIEITHNTPEEFINTVAAIADTFGGINLEDIKAPECFVIERELIARCKVPVFHD
DQHGTAIVTAAGMINALEIQGKLISDATFVCMGAGAAAIACMTMLVKCGANRANIYMLDTKGVIHSGRTDLNEYKALFAN
DTDKRTLLDVIKGADVFLGLSGPDVIGAEEVALMADKPVIFACSNPDPEIKPALAHATRSDLIMGTGRSDYPNQVNNVLC
FPFIFRGALDVRASCINDEMKIAAVHAIAALAKEPVPASVLKAYPNVTSLSFGPEYVLPKPMDPRLLPNVARAVAQAAID
SGVAAIPTLPKYAD
>Mature_413_residues
SDIRQQALDYHEFPIPGKTAVCLTKPAETSHDLALAYSPGVAEPVREIAANPDNAYRYTNKGNTVAVITNGTAILGLGNL
GPLASKPVMEGKALLFKHFANIDSTDIEITHNTPEEFINTVAAIADTFGGINLEDIKAPECFVIERELIARCKVPVFHDD
QHGTAIVTAAGMINALEIQGKLISDATFVCMGAGAAAIACMTMLVKCGANRANIYMLDTKGVIHSGRTDLNEYKALFAND
TDKRTLLDVIKGADVFLGLSGPDVIGAEEVALMADKPVIFACSNPDPEIKPALAHATRSDLIMGTGRSDYPNQVNNVLCF
PFIFRGALDVRASCINDEMKIAAVHAIAALAKEPVPASVLKAYPNVTSLSFGPEYVLPKPMDPRLLPNVARAVAQAAIDS
GVAAIPTLPKYAD

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=368, Percent_Identity=26.6304347826087, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI62420880, Length=368, Percent_Identity=26.6304347826087, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI239049447, Length=368, Percent_Identity=26.6304347826087, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI4505143, Length=341, Percent_Identity=26.099706744868, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1788806, Length=407, Percent_Identity=60.4422604422604, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI87081919, Length=291, Percent_Identity=26.4604810996564, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17537199, Length=370, Percent_Identity=27.8378378378378, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356279, Length=407, Percent_Identity=26.044226044226, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281362674, Length=407, Percent_Identity=26.044226044226, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281362672, Length=407, Percent_Identity=26.044226044226, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24646388, Length=365, Percent_Identity=27.6712328767123, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24646386, Length=365, Percent_Identity=27.6712328767123, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI78707242, Length=342, Percent_Identity=26.0233918128655, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI78707238, Length=342, Percent_Identity=26.0233918128655, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI78707240, Length=342, Percent_Identity=26.0233918128655, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI281363505, Length=356, Percent_Identity=23.876404494382, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI78707236, Length=356, Percent_Identity=23.876404494382, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281363503, Length=356, Percent_Identity=23.876404494382, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI78707232, Length=356, Percent_Identity=23.876404494382, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 44026; Mature: 43895

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00331 MALIC_ENZYMES ; PS00141 ASP_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIRQQALDYHEFPIPGKTAVCLTKPAETSHDLALAYSPGVAEPVREIAANPDNAYRYT
CCHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCHHHHHHHHHCCCCCCEEEE
NKGNTVAVITNGTAILGLGNLGPLASKPVMEGKALLFKHFANIDSTDIEITHNTPEEFIN
CCCCEEEEEECCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHH
TVAAIADTFGGINLEDIKAPECFVIERELIARCKVPVFHDDQHGTAIVTAAGMINALEIQ
HHHHHHHHHCCCCHHHCCCCCEEEEEHHHHHHCCCCEEECCCCCEEEEEECCCEEEEEEC
GKLISDATFVCMGAGAAAIACMTMLVKCGANRANIYMLDTKGVIHSGRTDLNEYKALFAN
CEEECCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCEECCCCCHHHHHHHHCC
DTDKRTLLDVIKGADVFLGLSGPDVIGAEEVALMADKPVIFACSNPDPEIKPALAHATRS
CCCHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEECCCEEEEECCCCCCCCHHHHHCCCC
DLIMGTGRSDYPNQVNNVLCFPFIFRGALDVRASCINDEMKIAAVHAIAALAKEPVPASV
CEEEECCCCCCCHHHHCEEEEHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHH
LKAYPNVTSLSFGPEYVLPKPMDPRLLPNVARAVAQAAIDSGVAAIPTLPKYAD
HHHCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCC
>Mature Secondary Structure 
SDIRQQALDYHEFPIPGKTAVCLTKPAETSHDLALAYSPGVAEPVREIAANPDNAYRYT
CHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCHHHHHHHHHCCCCCCEEEE
NKGNTVAVITNGTAILGLGNLGPLASKPVMEGKALLFKHFANIDSTDIEITHNTPEEFIN
CCCCEEEEEECCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHH
TVAAIADTFGGINLEDIKAPECFVIERELIARCKVPVFHDDQHGTAIVTAAGMINALEIQ
HHHHHHHHHCCCCHHHCCCCCEEEEEHHHHHHCCCCEEECCCCCEEEEEECCCEEEEEEC
GKLISDATFVCMGAGAAAIACMTMLVKCGANRANIYMLDTKGVIHSGRTDLNEYKALFAN
CEEECCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCEECCCCCHHHHHHHHCC
DTDKRTLLDVIKGADVFLGLSGPDVIGAEEVALMADKPVIFACSNPDPEIKPALAHATRS
CCCHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEECCCEEEEECCCCCCCCHHHHHCCCC
DLIMGTGRSDYPNQVNNVLCFPFIFRGALDVRASCINDEMKIAAVHAIAALAKEPVPASV
CEEEECCCCCCCHHHHCEEEEHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHH
LKAYPNVTSLSFGPEYVLPKPMDPRLLPNVARAVAQAAIDSGVAAIPTLPKYAD
HHHCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 36376 [H]