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The map label for this gene is insG [H]

Identifier: 126175999

GI number: 126175999

Start: 4472091

End: 4473413

Strand: Reverse

Name: insG [H]

Synonym: Sbal_3808

Alternate gene names: 126175999

Gene position: 4473413-4472091 (Counterclockwise)

Preceding gene: 126176000

Following gene: 126175996

Centisome position: 87.25

GC content: 48.22

Gene sequence:

>1323_bases
ATGCAACTCTCAGAAGCACTCGCCCGCACTCATATTACCCGCCTAACCGAATTCACCTGCTTAGCTGATGTGTTAGAACC
TGAGTTAATTCAATCTTGTTTAGACTCACAAGGCGTTGCGACGTTAAGACGGCGTAAATTGCCGATGGACGCGATGATTT
GGGCCGTTATCGGAATGGCTTTGTTCCGAGGGGAGTCTGTTCGGTCGCTCATCAACAAACTCGACATTGTTTTGCCGCAA
GAGATTGATTATGTTGCACGCAGCGCCGTGACTCAGGCGCGTAAACGATTGGGCAGTGAGGTTATTCGAGAAGTGTTTAG
TCGCAGCGCCAATACCTGGCACGCGAGAGCTGAACATCCTCATTGGTGCGGTCTTAACTTGTATGGTGTTGACGGTGTAG
TGTGGCGGACACCCGATAGTGTCCAAAATCAAGCCGCCTTTGCACGAACCGCAAACGCTTCAGGCGAAGCGGTTTATCCG
CAAATTCGGATGGTTTGCTTGATGGAGTTAACCAGCCATTTGTTGGTCAATAGCGCCTTTGATTCGGTTGCCGAAAATGA
AATGAACTTAGCATCTCAGCTCATTCCTAGCATTCCCAATCACAGCCTAACCCTATTTGACCGAGGATTTTACTCGCTCG
GCCTGTTACACGCTTGGCAGCAAGCGCAACCTGACAGCCACTGGTTGTTGCCATTGAAGAAAGGCACTCAATATGAGGTG
GTTCGAACATTGGGAAAACATGACCAGTGGGTGAAACTCACCACCACGCCTCAAGCCAGAAAGAAATGGCCGCAGCTCCC
TGATACCCTTGAAGCGCGTTTACTCGCTAAGACAGTGAAAGGTAAGTCAGTCGCCATTTTGACCTCGTTAACTGACCCGA
TGCGTTACCCCAGCGAAGACATCGTCGACTTATATGCCCATCGATGGGAAATCGAACGGGGCTACAGAGAGATGAAACAA
CATCTGTTGGAGAGTCGTTTTACGCTACGCAGTCAACTACCGGAGCTGGTGACTCAAGAGTTGTGGGGCGTGCTACTGGC
CTATAATCTCATTAGATATAAGATGTTGCTGATGGCTAAAAGCTTGCCATCGGTCCATCCTAATCAACTGAGTTTTAGGG
ATGCGGCGAGTTATATCATCTTCAAGTTGACACAACTGCCATCACAAACACCGGGGAATGTTCCGAGAGACGTATTGGAT
ATAGAACGAAATGCACGGCAATTTAAGTTGGATGGTAAACGAGAAAGGGCTTATCCAAGAGTCCTTAAAATGAGCAAAAA
CAAGTTTCCGGTTAGACCAAAGAAAAATGCCGTTCACTCTTAA

Upstream 100 bases:

>100_bases
AAGACCCGCATTCGCGTAATGCCGATCAGTTAAGACAGATCACTTGACGATCTCGCTGAAAAGATCAAAATCCGATGACC
TCTTGTCATCGGATTTTTTT

Downstream 100 bases:

>100_bases
GTGAACGGCATTACGCATTCGCGGGTCTTTTGCTTATGTGTTAATTAGCTATTATTGCAGCAGAACATTGAACTTCAGTA
GGGTTTGGACACCACGAGGT

Product: transposase, IS4 family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MQLSEALARTHITRLTEFTCLADVLEPELIQSCLDSQGVATLRRRKLPMDAMIWAVIGMALFRGESVRSLINKLDIVLPQ
EIDYVARSAVTQARKRLGSEVIREVFSRSANTWHARAEHPHWCGLNLYGVDGVVWRTPDSVQNQAAFARTANASGEAVYP
QIRMVCLMELTSHLLVNSAFDSVAENEMNLASQLIPSIPNHSLTLFDRGFYSLGLLHAWQQAQPDSHWLLPLKKGTQYEV
VRTLGKHDQWVKLTTTPQARKKWPQLPDTLEARLLAKTVKGKSVAILTSLTDPMRYPSEDIVDLYAHRWEIERGYREMKQ
HLLESRFTLRSQLPELVTQELWGVLLAYNLIRYKMLLMAKSLPSVHPNQLSFRDAASYIIFKLTQLPSQTPGNVPRDVLD
IERNARQFKLDGKRERAYPRVLKMSKNKFPVRPKKNAVHS

Sequences:

>Translated_440_residues
MQLSEALARTHITRLTEFTCLADVLEPELIQSCLDSQGVATLRRRKLPMDAMIWAVIGMALFRGESVRSLINKLDIVLPQ
EIDYVARSAVTQARKRLGSEVIREVFSRSANTWHARAEHPHWCGLNLYGVDGVVWRTPDSVQNQAAFARTANASGEAVYP
QIRMVCLMELTSHLLVNSAFDSVAENEMNLASQLIPSIPNHSLTLFDRGFYSLGLLHAWQQAQPDSHWLLPLKKGTQYEV
VRTLGKHDQWVKLTTTPQARKKWPQLPDTLEARLLAKTVKGKSVAILTSLTDPMRYPSEDIVDLYAHRWEIERGYREMKQ
HLLESRFTLRSQLPELVTQELWGVLLAYNLIRYKMLLMAKSLPSVHPNQLSFRDAASYIIFKLTQLPSQTPGNVPRDVLD
IERNARQFKLDGKRERAYPRVLKMSKNKFPVRPKKNAVHS
>Mature_440_residues
MQLSEALARTHITRLTEFTCLADVLEPELIQSCLDSQGVATLRRRKLPMDAMIWAVIGMALFRGESVRSLINKLDIVLPQ
EIDYVARSAVTQARKRLGSEVIREVFSRSANTWHARAEHPHWCGLNLYGVDGVVWRTPDSVQNQAAFARTANASGEAVYP
QIRMVCLMELTSHLLVNSAFDSVAENEMNLASQLIPSIPNHSLTLFDRGFYSLGLLHAWQQAQPDSHWLLPLKKGTQYEV
VRTLGKHDQWVKLTTTPQARKKWPQLPDTLEARLLAKTVKGKSVAILTSLTDPMRYPSEDIVDLYAHRWEIERGYREMKQ
HLLESRFTLRSQLPELVTQELWGVLLAYNLIRYKMLLMAKSLPSVHPNQLSFRDAASYIIFKLTQLPSQTPGNVPRDVLD
IERNARQFKLDGKRERAYPRVLKMSKNKFPVRPKKNAVHS

Specific function: Involved in the transposition of the insertion sequence IS4 [H]

COG id: COG3385

COG function: function code L; FOG: Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1790729, Length=419, Percent_Identity=52.9832935560859, Blast_Score=471, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002559 [H]

Pfam domain/function: PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 50279; Mature: 50279

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLSEALARTHITRLTEFTCLADVLEPELIQSCLDSQGVATLRRRKLPMDAMIWAVIGMA
CCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHH
LFRGESVRSLINKLDIVLPQEIDYVARSAVTQARKRLGSEVIREVFSRSANTWHARAEHP
HHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
HWCGLNLYGVDGVVWRTPDSVQNQAAFARTANASGEAVYPQIRMVCLMELTSHLLVNSAF
CCCCCEEECCCCEEECCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
DSVAENEMNLASQLIPSIPNHSLTLFDRGFYSLGLLHAWQQAQPDSHWLLPLKKGTQYEV
HHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHH
VRTLGKHDQWVKLTTTPQARKKWPQLPDTLEARLLAKTVKGKSVAILTSLTDPMRYPSED
HHHHCCCCCEEEEECCCHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHH
IVDLYAHRWEIERGYREMKQHLLESRFTLRSQLPELVTQELWGVLLAYNLIRYKMLLMAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLPSVHPNQLSFRDAASYIIFKLTQLPSQTPGNVPRDVLDIERNARQFKLDGKRERAYPR
HCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHCCCCCCHHHHHHH
VLKMSKNKFPVRPKKNAVHS
HHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQLSEALARTHITRLTEFTCLADVLEPELIQSCLDSQGVATLRRRKLPMDAMIWAVIGMA
CCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHH
LFRGESVRSLINKLDIVLPQEIDYVARSAVTQARKRLGSEVIREVFSRSANTWHARAEHP
HHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
HWCGLNLYGVDGVVWRTPDSVQNQAAFARTANASGEAVYPQIRMVCLMELTSHLLVNSAF
CCCCCEEECCCCEEECCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
DSVAENEMNLASQLIPSIPNHSLTLFDRGFYSLGLLHAWQQAQPDSHWLLPLKKGTQYEV
HHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHH
VRTLGKHDQWVKLTTTPQARKKWPQLPDTLEARLLAKTVKGKSVAILTSLTDPMRYPSED
HHHHCCCCCEEEEECCCHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHH
IVDLYAHRWEIERGYREMKQHLLESRFTLRSQLPELVTQELWGVLLAYNLIRYKMLLMAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLPSVHPNQLSFRDAASYIIFKLTQLPSQTPGNVPRDVLDIERNARQFKLDGKRERAYPR
HCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHCCCCCCHHHHHHH
VLKMSKNKFPVRPKKNAVHS
HHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6268937; 7610040; 9278503 [H]