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The map label for this gene is ptsN [H]

Identifier: 126175865

GI number: 126175865

Start: 4289682

End: 4290125

Strand: Direct

Name: ptsN [H]

Synonym: Sbal_3670

Alternate gene names: 126175865

Gene position: 4289682-4290125 (Clockwise)

Preceding gene: 126175864

Following gene: 126175866

Centisome position: 83.66

GC content: 48.42

Gene sequence:

>444_bases
ATGGAACTTTGTACCATACTGCGGCCGGAGTGCACTACCTGCGCCACTCCGGGCAGCAAGAAAAAGGTACTGGAACTCAT
CAGCGACTTAGTCGCTGCCCAGTACCCGACCCTCTCATCTCAAGAGATATTCGAAAGCCTAGTGGCGCGCGAAAAGATGG
GGAGCACAGGTATAGGTAACGGCATCGCCATTCCACATGGTCGACTAGCCGATATAGATCAGCCCATTGCCATACTCATT
AAGTGTGAAGAGCCGATAGCATTCGATGCTATCGATAAGCAACCTGTTGATATTTTATTTGCACTACTGGTGCCTGCAGA
CCAATGTCAGCAGCACTTAAGTACCTTGTCTTGCATGGCCGAAAAGCTGAGCGACAAGCAAATATTAAAGCAATTACGCA
AGACGCATGATGAAACGGAACTCTATCAGGTAATCACTGGATGA

Upstream 100 bases:

>100_bases
CTTCAGAGCATGCTGACATGTATGCCGCTATAGACGCCCTGATTGACAAGCTTGATCGTCAGGTTATTAAACACAAAGAG
AAGTTAACAAAACACTAATA

Downstream 100 bases:

>100_bases
AACTGGTCATAGTGTCGGGCCGCTCAGGCTCAGGCAAATCTGTCGCCCTAAGGGTACTCGAAGATCTCGGCTATTATTGT
GTCGATAATCTTCCCTTGCC

Product: PTS IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MELCTILRPECTTCATPGSKKKVLELISDLVAAQYPTLSSQEIFESLVAREKMGSTGIGNGIAIPHGRLADIDQPIAILI
KCEEPIAFDAIDKQPVDILFALLVPADQCQQHLSTLSCMAEKLSDKQILKQLRKTHDETELYQVITG

Sequences:

>Translated_147_residues
MELCTILRPECTTCATPGSKKKVLELISDLVAAQYPTLSSQEIFESLVAREKMGSTGIGNGIAIPHGRLADIDQPIAILI
KCEEPIAFDAIDKQPVDILFALLVPADQCQQHLSTLSCMAEKLSDKQILKQLRKTHDETELYQVITG
>Mature_147_residues
MELCTILRPECTTCATPGSKKKVLELISDLVAAQYPTLSSQEIFESLVAREKMGSTGIGNGIAIPHGRLADIDQPIAILI
KCEEPIAFDAIDKQPVDILFALLVPADQCQQHLSTLSCMAEKLSDKQILKQLRKTHDETELYQVITG

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=148, Percent_Identity=54.0540540540541, Blast_Score=157, Evalue=4e-40,
Organism=Escherichia coli, GI1786951, Length=129, Percent_Identity=23.2558139534884, Blast_Score=64, Evalue=4e-12,
Organism=Escherichia coli, GI2367327, Length=95, Percent_Identity=30.5263157894737, Blast_Score=62, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16121; Mature: 16121

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELCTILRPECTTCATPGSKKKVLELISDLVAAQYPTLSSQEIFESLVAREKMGSTGIGN
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCC
GIAIPHGRLADIDQPIAILIKCEEPIAFDAIDKQPVDILFALLVPADQCQQHLSTLSCMA
CCCCCCCCCCCCCCCEEEEEECCCCCEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHH
EKLSDKQILKQLRKTHDETELYQVITG
HHCCHHHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MELCTILRPECTTCATPGSKKKVLELISDLVAAQYPTLSSQEIFESLVAREKMGSTGIGN
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCC
GIAIPHGRLADIDQPIAILIKCEEPIAFDAIDKQPVDILFALLVPADQCQQHLSTLSCMA
CCCCCCCCCCCCCCCEEEEEECCCCCEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHH
EKLSDKQILKQLRKTHDETELYQVITG
HHCCHHHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2695747 [H]