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The map label for this gene is rpoN [H]

Identifier: 126175863

GI number: 126175863

Start: 4287885

End: 4289363

Strand: Direct

Name: rpoN [H]

Synonym: Sbal_3668

Alternate gene names: 126175863

Gene position: 4287885-4289363 (Clockwise)

Preceding gene: 126175862

Following gene: 126175864

Centisome position: 83.63

GC content: 48.14

Gene sequence:

>1479_bases
ATGAAAGCGTCACTCCAGCTCAAACTGGGTCAACAGTTAACCATGACGCCACAATTACAACAGGCCATACGTCTGTTGCA
ACTGTCGTCATTAGAACTGCAGCAAGAGATCCAGCAAGCCTTAGATTCTAATCCCCTTCTTGAGCTGGAAGAGGATCAAT
TCGACGCCACTAAAGACCCTGTAAACCAAACGCTATCCGATACGGATTTCAGTGAATCTAGCCCCCTGCCGCCAGAAAAA
GACAGCTCCACCATAGACACGGCAGAGTCGCTCACGAAAGAGTCCATGCCCGACGAACTCCCCATGGACACCACATGGGA
TGAAGTGTTCACCGCCTCTCCTAATTCTGGCCCCGGCGGCAGTCGTGAAGACGATATGCCCTTCCAAGGTGAGACCAGTG
AAGGCTTGTATGAACACTTAGAATGGCAAAAAAACCTCACACCGTTTTCTGAAACCGATTTAGCCATTGCCACCGCCATT
ATTGATGCCATCGACGATCAAGGCTATCTCACCCAAAGCACTGACGAAATCCTCGAAGCCATGGGCAATTCTGAGGTTGA
GCTCGACGAAGTCGAAGCCGTACTCAAGCGCATTCAACATTTTGATCCCATTGGTGTTGCCGCCCGCGATTTAAGCGAGT
GCCTGTTGATCCAACTGTCGCATTTCGCCGCTGACACGCCCAACCTAGAAAACGCCCGCATGCTGATCAAAGATCATTTA
GATCTGATCGCCGCCCGCGATTTTAGATTGTTGATGCGTAAGACTAAATTAAAAGAGGATGAATTAAGAGACGGCATCGC
GCTTATCCAAACCCTCAATCCCCGCCCAGGTTTATTGATTACGCCCAACGATGACGAATACGTCATCCCAGATGTGTCAG
TGTCGAAGAAAAATGGCCGCTGGGTGGTTGAGCTGAATCCTGACTGCATGCCAAAAATCAATGTAAATCAACATTATGCT
TCGATGGCGCGCAGCACTAAAAGCCAAGCCGATAGCCAATTTATTCGTGGCCATCTGCAAGAGGCTAAATGGTTTATCAA
GAGTTTAGAGAGCCGTAATGATACCCTGTTGAAGGTGGCGAACTGTATCGTCCAATATCAGCAAGGTTTCTTTGAATACG
GTGAAGAAGCCATGAAACCTATGGTGCTTAACGATATTGCCGAAGCGGTAGAAATGCATGAGTCGACCATCTCACGAGTG
ACGACACAGAAATACATGCATACCCCTAAGGGACTTTTTGAACTAAAATACTTCTTCTCTAGCCACGTCGGTACTGACGA
TGGTGGGGAGTGCTCATCCACAGCAATCAGAGCCTTCATTAAGAAACTGGTTGCAGCGGAAAACCAGAAAAAACCATTGA
GCGACAGTAAGATGGCGCAACTTCTGGCAGAACAAGGGATCAACGTAGCCCGCAGAACCATTGCGAAGTACCGAGAGGCT
ATGCTCATTCCGCCTTCTAATCAGCGTAAAAGTTTATAA

Upstream 100 bases:

>100_bases
AGCGGAGCCAATGCAGGCAATTTATGCCACCATTGGACTCCACTCAAGATGATAACAAGCGACCTCATCCAACAATAATA
ACGTAGGGATCAGCGGTAAC

Downstream 100 bases:

>100_bases
CCTACAGTGGATATCGGAGGACGTTTCTATGCAAATAAACCTGAGTGGACACCATGTCGAAATTACCGAATCGTTACGCG
CATATGTAGAAGAAAAGTTT

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MKASLQLKLGQQLTMTPQLQQAIRLLQLSSLELQQEIQQALDSNPLLELEEDQFDATKDPVNQTLSDTDFSESSPLPPEK
DSSTIDTAESLTKESMPDELPMDTTWDEVFTASPNSGPGGSREDDMPFQGETSEGLYEHLEWQKNLTPFSETDLAIATAI
IDAIDDQGYLTQSTDEILEAMGNSEVELDEVEAVLKRIQHFDPIGVAARDLSECLLIQLSHFAADTPNLENARMLIKDHL
DLIAARDFRLLMRKTKLKEDELRDGIALIQTLNPRPGLLITPNDDEYVIPDVSVSKKNGRWVVELNPDCMPKINVNQHYA
SMARSTKSQADSQFIRGHLQEAKWFIKSLESRNDTLLKVANCIVQYQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRV
TTQKYMHTPKGLFELKYFFSSHVGTDDGGECSSTAIRAFIKKLVAAENQKKPLSDSKMAQLLAEQGINVARRTIAKYREA
MLIPPSNQRKSL

Sequences:

>Translated_492_residues
MKASLQLKLGQQLTMTPQLQQAIRLLQLSSLELQQEIQQALDSNPLLELEEDQFDATKDPVNQTLSDTDFSESSPLPPEK
DSSTIDTAESLTKESMPDELPMDTTWDEVFTASPNSGPGGSREDDMPFQGETSEGLYEHLEWQKNLTPFSETDLAIATAI
IDAIDDQGYLTQSTDEILEAMGNSEVELDEVEAVLKRIQHFDPIGVAARDLSECLLIQLSHFAADTPNLENARMLIKDHL
DLIAARDFRLLMRKTKLKEDELRDGIALIQTLNPRPGLLITPNDDEYVIPDVSVSKKNGRWVVELNPDCMPKINVNQHYA
SMARSTKSQADSQFIRGHLQEAKWFIKSLESRNDTLLKVANCIVQYQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRV
TTQKYMHTPKGLFELKYFFSSHVGTDDGGECSSTAIRAFIKKLVAAENQKKPLSDSKMAQLLAEQGINVARRTIAKYREA
MLIPPSNQRKSL
>Mature_492_residues
MKASLQLKLGQQLTMTPQLQQAIRLLQLSSLELQQEIQQALDSNPLLELEEDQFDATKDPVNQTLSDTDFSESSPLPPEK
DSSTIDTAESLTKESMPDELPMDTTWDEVFTASPNSGPGGSREDDMPFQGETSEGLYEHLEWQKNLTPFSETDLAIATAI
IDAIDDQGYLTQSTDEILEAMGNSEVELDEVEAVLKRIQHFDPIGVAARDLSECLLIQLSHFAADTPNLENARMLIKDHL
DLIAARDFRLLMRKTKLKEDELRDGIALIQTLNPRPGLLITPNDDEYVIPDVSVSKKNGRWVVELNPDCMPKINVNQHYA
SMARSTKSQADSQFIRGHLQEAKWFIKSLESRNDTLLKVANCIVQYQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRV
TTQKYMHTPKGLFELKYFFSSHVGTDDGGECSSTAIRAFIKKLVAAENQKKPLSDSKMAQLLAEQGINVARRTIAKYREA
MLIPPSNQRKSL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor recognizes a DNA element, designated as region A upstream of the pressure-regulated operon [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=494, Percent_Identity=63.1578947368421, Blast_Score=632, Evalue=0.0,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 55239; Mature: 55239

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASLQLKLGQQLTMTPQLQQAIRLLQLSSLELQQEIQQALDSNPLLELEEDQFDATKDP
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCH
VNQTLSDTDFSESSPLPPEKDSSTIDTAESLTKESMPDELPMDTTWDEVFTASPNSGPGG
HHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHEECCCCCCCCC
SREDDMPFQGETSEGLYEHLEWQKNLTPFSETDLAIATAIIDAIDDQGYLTQSTDEILEA
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEECCHHHHHHH
MGNSEVELDEVEAVLKRIQHFDPIGVAARDLSECLLIQLSHFAADTPNLENARMLIKDHL
HCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
DLIAARDFRLLMRKTKLKEDELRDGIALIQTLNPRPGLLITPNDDEYVIPDVSVSKKNGR
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEECCCCCCEECCCCEEECCCCE
WVVELNPDCMPKINVNQHYASMARSTKSQADSQFIRGHLQEAKWFIKSLESRNDTLLKVA
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
NCIVQYQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPKGLFELKYFFS
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
SHVGTDDGGECSSTAIRAFIKKLVAAENQKKPLSDSKMAQLLAEQGINVARRTIAKYREA
HCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHE
MLIPPSNQRKSL
EECCCCCCCCCC
>Mature Secondary Structure
MKASLQLKLGQQLTMTPQLQQAIRLLQLSSLELQQEIQQALDSNPLLELEEDQFDATKDP
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCH
VNQTLSDTDFSESSPLPPEKDSSTIDTAESLTKESMPDELPMDTTWDEVFTASPNSGPGG
HHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHEECCCCCCCCC
SREDDMPFQGETSEGLYEHLEWQKNLTPFSETDLAIATAIIDAIDDQGYLTQSTDEILEA
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEECCHHHHHHH
MGNSEVELDEVEAVLKRIQHFDPIGVAARDLSECLLIQLSHFAADTPNLENARMLIKDHL
HCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
DLIAARDFRLLMRKTKLKEDELRDGIALIQTLNPRPGLLITPNDDEYVIPDVSVSKKNGR
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEECCCCCCEECCCCEEECCCCE
WVVELNPDCMPKINVNQHYASMARSTKSQADSQFIRGHLQEAKWFIKSLESRNDTLLKVA
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
NCIVQYQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPKGLFELKYFFS
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
SHVGTDDGGECSSTAIRAFIKKLVAAENQKKPLSDSKMAQLLAEQGINVARRTIAKYREA
HCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHE
MLIPPSNQRKSL
EECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10760597 [H]