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The map label for this gene is dnaB [H]

Identifier: 126175833

GI number: 126175833

Start: 4259932

End: 4261338

Strand: Reverse

Name: dnaB [H]

Synonym: Sbal_3638

Alternate gene names: 126175833

Gene position: 4261338-4259932 (Counterclockwise)

Preceding gene: 126175835

Following gene: 126175832

Centisome position: 83.11

GC content: 49.32

Gene sequence:

>1407_bases
ATGTCACAACAAGGTGCTTTTAAGCCTAAAAGTAAGCCAAGAGATTTGCAGCTTGATGGACTCAAGCTGCCACCGCATTC
AATTGAGGCTGAACAATCGGTATTAGGCGGCTTGATGTTAGATGCCGAAGCGTGGGACAGGGTCGCTGAGGCCGTCGTAA
AAGATGACTTTTACTCCCGTTCACACAAGATGATCTTCTCTGCCATGCACAGATTGGTCGATCTGGGGCAGCCGATCGAT
TTGATCACTGTTTCTGAACAGCTCGAAGTTGAAAATCAATTAGAAGATGCCGGCGGTTTTGCCTATTTAGGTGAGATTGC
GAAAAATACCCCAAGTGCGGGTAACATTTTATCTTACGCCGAAATCGTCCGTGAACGCGCCGTAGTGCGTGACATGATCC
GCGTAGCGCACGAAATTGCCGATGCGGGTTACAACCCTGAAGGCCGCGATTCGAGCGAATTACTCGACCTTGCAGAGAGT
AAAGTCTTCAAGATCGCTGAGATGCGCACTAATTCCAATGAAGGTCCCGAAGGCATTAAAACCATTCTGGAAAAAACCGT
TGATAAGATTGAGCAGCTTTATAATAACCCCCATAACGGTGTGACTGGGGTTTCGAGCGGCTTTAACGATCTGGATAAAA
TGACCGCTGGCTTCCAGTCCGGTGATTTGATTATCGTTGCGGCGCGTCCTTCTATGGGTAAAACCACCTTCGCGATGAAC
TTGTGCGAACAAGCCGCGATGAATGAAGACAAGCCGGTGCTTATCTTCAGTCTCGAGATGCCGTCAGAACAGATTATGAT
GCGTATGTTGGCCTCCTTGGGCCGCGTCGACCAAACTAAAATCCGTACTGGCCAGCTCGATGACGAGGATTGGGCGCGGG
TTTCATCGACCATGGGCATCATGCTCGAGCAAGGTAAAATGTATATCGATGACGGTTCGGGGTTAACGCCGACCGAGGTT
CGTAGCCGCGCGCGTCGTATCGCCCGTGAGTACGGTGGTCTATCTATGATCATGATAGATTACCTGCAGTTAATGCAGGT
GCCCGCCTTGAAAGATAACCGTACCTTAGAGATTTCTGAGATTTCACGCTCCCTAAAGGCGTTAGCCAAAGAGCTAGAAA
TCCCGATTATTGCCCTGTCGCAGCTTAACCGCTCGCTCGAGCAAAGGGCCGATAAACGCCCGGTAAACTCGGATTTGCGT
GAATCGGGCGCTATCGAGCAGGATGCGGATCTCATCATGTTTATTTATCGCGATGAAGTGTATAACAACGATTCTCAAGA
TAAAGGTACTGCCGAAATCATTATTGGTAAGCAACGTAACGGCCCGATTGGTCGGGTGCGACTCACCTTCCAAGGGCAGT
TTTCGCGCTTCGATAATTATGCCGGACCTCAGTTTGAAGAAGACTAA

Upstream 100 bases:

>100_bases
TGTCCGTTGGAATGAGATAACAGGCGAGTGTAAACATCCTGTTTGCTTGACGCGGGTTTTATATCATATTTTGTTAAGTT
GTTGGAAATCGGGAGCGTTA

Downstream 100 bases:

>100_bases
ATTTACGTTTGAAAATTGTAAATTTGCGTATGCAGAAGACGTGCTGTGCTGATGTATTTTTTATATCCGTGACTTTTTAT
TTCCTGAGTGAGCAAGGTAA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MSQQGAFKPKSKPRDLQLDGLKLPPHSIEAEQSVLGGLMLDAEAWDRVAEAVVKDDFYSRSHKMIFSAMHRLVDLGQPID
LITVSEQLEVENQLEDAGGFAYLGEIAKNTPSAGNILSYAEIVRERAVVRDMIRVAHEIADAGYNPEGRDSSELLDLAES
KVFKIAEMRTNSNEGPEGIKTILEKTVDKIEQLYNNPHNGVTGVSSGFNDLDKMTAGFQSGDLIIVAARPSMGKTTFAMN
LCEQAAMNEDKPVLIFSLEMPSEQIMMRMLASLGRVDQTKIRTGQLDDEDWARVSSTMGIMLEQGKMYIDDGSGLTPTEV
RSRARRIAREYGGLSMIMIDYLQLMQVPALKDNRTLEISEISRSLKALAKELEIPIIALSQLNRSLEQRADKRPVNSDLR
ESGAIEQDADLIMFIYRDEVYNNDSQDKGTAEIIIGKQRNGPIGRVRLTFQGQFSRFDNYAGPQFEED

Sequences:

>Translated_468_residues
MSQQGAFKPKSKPRDLQLDGLKLPPHSIEAEQSVLGGLMLDAEAWDRVAEAVVKDDFYSRSHKMIFSAMHRLVDLGQPID
LITVSEQLEVENQLEDAGGFAYLGEIAKNTPSAGNILSYAEIVRERAVVRDMIRVAHEIADAGYNPEGRDSSELLDLAES
KVFKIAEMRTNSNEGPEGIKTILEKTVDKIEQLYNNPHNGVTGVSSGFNDLDKMTAGFQSGDLIIVAARPSMGKTTFAMN
LCEQAAMNEDKPVLIFSLEMPSEQIMMRMLASLGRVDQTKIRTGQLDDEDWARVSSTMGIMLEQGKMYIDDGSGLTPTEV
RSRARRIAREYGGLSMIMIDYLQLMQVPALKDNRTLEISEISRSLKALAKELEIPIIALSQLNRSLEQRADKRPVNSDLR
ESGAIEQDADLIMFIYRDEVYNNDSQDKGTAEIIIGKQRNGPIGRVRLTFQGQFSRFDNYAGPQFEED
>Mature_467_residues
SQQGAFKPKSKPRDLQLDGLKLPPHSIEAEQSVLGGLMLDAEAWDRVAEAVVKDDFYSRSHKMIFSAMHRLVDLGQPIDL
ITVSEQLEVENQLEDAGGFAYLGEIAKNTPSAGNILSYAEIVRERAVVRDMIRVAHEIADAGYNPEGRDSSELLDLAESK
VFKIAEMRTNSNEGPEGIKTILEKTVDKIEQLYNNPHNGVTGVSSGFNDLDKMTAGFQSGDLIIVAARPSMGKTTFAMNL
CEQAAMNEDKPVLIFSLEMPSEQIMMRMLASLGRVDQTKIRTGQLDDEDWARVSSTMGIMLEQGKMYIDDGSGLTPTEVR
SRARRIAREYGGLSMIMIDYLQLMQVPALKDNRTLEISEISRSLKALAKELEIPIIALSQLNRSLEQRADKRPVNSDLRE
SGAIEQDADLIMFIYRDEVYNNDSQDKGTAEIIIGKQRNGPIGRVRLTFQGQFSRFDNYAGPQFEED

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=463, Percent_Identity=74.5140388768899, Blast_Score=712, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 52177; Mature: 52046

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQGAFKPKSKPRDLQLDGLKLPPHSIEAEQSVLGGLMLDAEAWDRVAEAVVKDDFYSR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
SHKMIFSAMHRLVDLGQPIDLITVSEQLEVENQLEDAGGFAYLGEIAKNTPSAGNILSYA
HHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHH
EIVRERAVVRDMIRVAHEIADAGYNPEGRDSSELLDLAESKVFKIAEMRTNSNEGPEGIK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
TILEKTVDKIEQLYNNPHNGVTGVSSGFNDLDKMTAGFQSGDLIIVAARPSMGKTTFAMN
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHH
LCEQAAMNEDKPVLIFSLEMPSEQIMMRMLASLGRVDQTKIRTGQLDDEDWARVSSTMGI
HHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHH
MLEQGKMYIDDGSGLTPTEVRSRARRIAREYGGLSMIMIDYLQLMQVPALKDNRTLEISE
EEECCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEHHH
ISRSLKALAKELEIPIIALSQLNRSLEQRADKRPVNSDLRESGAIEQDADLIMFIYRDEV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEEHHH
YNNDSQDKGTAEIIIGKQRNGPIGRVRLTFQGQFSRFDNYAGPQFEED
CCCCCCCCCCEEEEEECCCCCCCEEEEEEEECCHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SQQGAFKPKSKPRDLQLDGLKLPPHSIEAEQSVLGGLMLDAEAWDRVAEAVVKDDFYSR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
SHKMIFSAMHRLVDLGQPIDLITVSEQLEVENQLEDAGGFAYLGEIAKNTPSAGNILSYA
HHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHH
EIVRERAVVRDMIRVAHEIADAGYNPEGRDSSELLDLAESKVFKIAEMRTNSNEGPEGIK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
TILEKTVDKIEQLYNNPHNGVTGVSSGFNDLDKMTAGFQSGDLIIVAARPSMGKTTFAMN
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHH
LCEQAAMNEDKPVLIFSLEMPSEQIMMRMLASLGRVDQTKIRTGQLDDEDWARVSSTMGI
HHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHH
MLEQGKMYIDDGSGLTPTEVRSRARRIAREYGGLSMIMIDYLQLMQVPALKDNRTLEISE
EEECCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEHHH
ISRSLKALAKELEIPIIALSQLNRSLEQRADKRPVNSDLRESGAIEQDADLIMFIYRDEV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEEHHH
YNNDSQDKGTAEIIIGKQRNGPIGRVRLTFQGQFSRFDNYAGPQFEED
CCCCCCCCCCEEEEEECCCCCCCEEEEEEEECCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]