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The map label for this gene is macA [H]

Identifier: 126175733

GI number: 126175733

Start: 4158956

End: 4160206

Strand: Reverse

Name: macA [H]

Synonym: Sbal_3536

Alternate gene names: 126175733

Gene position: 4160206-4158956 (Counterclockwise)

Preceding gene: 126175734

Following gene: 126175732

Centisome position: 81.14

GC content: 50.92

Gene sequence:

>1251_bases
ATGGACACGACAATACCGATGAAAAAATCCTCAAAGCGTAAATTGCTGCTCGCGTTAAGCGGCTTAATCCTCCTGGGTGG
CGGTGCTTATTTTATGTGGCATAAGCCTGAAACGGCACCTACTTATGTGACTGAAGCGGTGCGCCGTGGCGATATCGAAA
ACTCAGTGCTGGCCAATGGCATGTTGCAGGCCTCTAAACTGGTGAGTGTGGGTGCGCAGGTATCGGGGCAAATCTTAAGT
TTGCCGCTGGCACTGGGGGATGAAGTAAAGAAAGGCGATTTGATTGCTCAGATTGATAGCCTAGCGCAGCAGAATAATTT
GAAGGATGCCCTCGCATCACTCAAGAGTATCAATGCCCAGTTCCGCGCCAAACAGGCGCAGATCCGCCAAGCCAAGTTGG
AGTTTAGCCGCCAGCAGCAAATGCTTGCCGACAAGGCCAGTTCCCGCGCCGATTATGAAGTGGCCGATGCTAATTTAACG
GTTTATCAAGCCGAGCTTGAGCAATTAGAAGCGCAAAAACAACAGGCTGAGATTAACGTCGACAGTGCCCGAATCGATTT
AGGTTATACCAAAATCACCGCGCCTATGGATGGCACTGTGGTGTATTCGGCGGTGGAAGTGGGGCAAACCGTTAACGCCA
ATCAAACGACGCCGACAATCGTGGAAATGGCGCAGCTGGATACCATGACAGTTAAAGCGCAGATTTCCGAAGCGGATGTC
GTCAATGTCCATCCGGGTCAAGCCGTGTATTTCACGGTTCTTGGCAAACCGAATCATCCCTACCGAGGCATCTTAAGGGC
GATTGAACCTGGGCCGATTTCGATGGATGGCGACGATAGTAATATGAGCTCTAGCGACTCAGACGCCATTTATTACCATG
GTCTGTTCGATGTTGAAAACCCTGACCGCACCTTGCGTATCGGCATGACGGCGCAGGTGTCCATAGTGTTAGCCAAGGCC
GATGATGCGCTATTAGTGCCTGCGCAAATATTACGTATAAAGCCGAGGGAGAAATCAGCGGCGCCAGAGGCTAAGTCTGG
CGAGCGTAAAGCCCGCAGTGGCCCGCAATATCAAGTGCCTGTGTTAGTCGATAATCAAGTGCAATATGTGGATGTCACTG
TGGGGATTAATAACAAAATCAATGCTGAAATCACCACTGGTTTGAAGGAGGGCGATCAAGTCGTGCTTGGCATGCCGTCC
GCCGATGATGGCAGCAGCAAATTCCGTCGTCAACCTAGCGTGAGGTTTTAA

Upstream 100 bases:

>100_bases
TAACGACACTTCCTTCGTCTACTGTCTTTGGCTAACTCTATATACTGTTGGAACAATTTCTATTCCTTAGCAAGATGCAT
GCTTGCTGTCTTTAGCATTG

Downstream 100 bases:

>100_bases
CAGTGACAACGTCATTACTTGAAATTTCAGGTTGTTATCGCACTTTTCAGGCGGGTGAGCAGCAACTCACTGTGCTGAAA
GATGTGAACCTGTCGATTGG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MDTTIPMKKSSKRKLLLALSGLILLGGGAYFMWHKPETAPTYVTEAVRRGDIENSVLANGMLQASKLVSVGAQVSGQILS
LPLALGDEVKKGDLIAQIDSLAQQNNLKDALASLKSINAQFRAKQAQIRQAKLEFSRQQQMLADKASSRADYEVADANLT
VYQAELEQLEAQKQQAEINVDSARIDLGYTKITAPMDGTVVYSAVEVGQTVNANQTTPTIVEMAQLDTMTVKAQISEADV
VNVHPGQAVYFTVLGKPNHPYRGILRAIEPGPISMDGDDSNMSSSDSDAIYYHGLFDVENPDRTLRIGMTAQVSIVLAKA
DDALLVPAQILRIKPREKSAAPEAKSGERKARSGPQYQVPVLVDNQVQYVDVTVGINNKINAEITTGLKEGDQVVLGMPS
ADDGSSKFRRQPSVRF

Sequences:

>Translated_416_residues
MDTTIPMKKSSKRKLLLALSGLILLGGGAYFMWHKPETAPTYVTEAVRRGDIENSVLANGMLQASKLVSVGAQVSGQILS
LPLALGDEVKKGDLIAQIDSLAQQNNLKDALASLKSINAQFRAKQAQIRQAKLEFSRQQQMLADKASSRADYEVADANLT
VYQAELEQLEAQKQQAEINVDSARIDLGYTKITAPMDGTVVYSAVEVGQTVNANQTTPTIVEMAQLDTMTVKAQISEADV
VNVHPGQAVYFTVLGKPNHPYRGILRAIEPGPISMDGDDSNMSSSDSDAIYYHGLFDVENPDRTLRIGMTAQVSIVLAKA
DDALLVPAQILRIKPREKSAAPEAKSGERKARSGPQYQVPVLVDNQVQYVDVTVGINNKINAEITTGLKEGDQVVLGMPS
ADDGSSKFRRQPSVRF
>Mature_416_residues
MDTTIPMKKSSKRKLLLALSGLILLGGGAYFMWHKPETAPTYVTEAVRRGDIENSVLANGMLQASKLVSVGAQVSGQILS
LPLALGDEVKKGDLIAQIDSLAQQNNLKDALASLKSINAQFRAKQAQIRQAKLEFSRQQQMLADKASSRADYEVADANLT
VYQAELEQLEAQKQQAEINVDSARIDLGYTKITAPMDGTVVYSAVEVGQTVNANQTTPTIVEMAQLDTMTVKAQISEADV
VNVHPGQAVYFTVLGKPNHPYRGILRAIEPGPISMDGDDSNMSSSDSDAIYYHGLFDVENPDRTLRIGMTAQVSIVLAKA
DDALLVPAQILRIKPREKSAAPEAKSGERKARSGPQYQVPVLVDNQVQYVDVTVGINNKINAEITTGLKEGDQVVLGMPS
ADDGSSKFRRQPSVRF

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=391, Percent_Identity=36.5728900255754, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI87082036, Length=222, Percent_Identity=29.7297297297297, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789665, Length=381, Percent_Identity=25.1968503937008, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789929, Length=267, Percent_Identity=25.8426966292135, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786668, Length=287, Percent_Identity=27.8745644599303, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 45083; Mature: 45083

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTTIPMKKSSKRKLLLALSGLILLGGGAYFMWHKPETAPTYVTEAVRRGDIENSVLANG
CCCCCCCCCCCCCEEEEEECCEEEECCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHH
MLQASKLVSVGAQVSGQILSLPLALGDEVKKGDLIAQIDSLAQQNNLKDALASLKSINAQ
HHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCHH
FRAKQAQIRQAKLEFSRQQQMLADKASSRADYEVADANLTVYQAELEQLEAQKQQAEINV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEHHHHHHHHHHHHHHEEEC
DSARIDLGYTKITAPMDGTVVYSAVEVGQTVNANQTTPTIVEMAQLDTMTVKAQISEADV
CCEEEEECEEEEECCCCCEEEEEEEHHCCCCCCCCCCCHHHHHHHHCEEEEEEEECCCCE
VNVHPGQAVYFTVLGKPNHPYRGILRAIEPGPISMDGDDSNMSSSDSDAIYYHGLFDVEN
EEECCCCEEEEEEECCCCCCHHHHHHHCCCCCCEECCCCCCCCCCCCCEEEEEEEEECCC
PDRTLRIGMTAQVSIVLAKADDALLVPAQILRIKPREKSAAPEAKSGERKARSGPQYQVP
CCCEEEEECEEEEEEEEEECCCCEECCHHHEEECCCCCCCCCCCCCCCHHHCCCCCEEEE
VLVDNQVQYVDVTVGINNKINAEITTGLKEGDQVVLGMPSADDGSSKFRRQPSVRF
EEECCCEEEEEEEEECCCEECEEEECCCCCCCEEEEECCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure
MDTTIPMKKSSKRKLLLALSGLILLGGGAYFMWHKPETAPTYVTEAVRRGDIENSVLANG
CCCCCCCCCCCCCEEEEEECCEEEECCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHH
MLQASKLVSVGAQVSGQILSLPLALGDEVKKGDLIAQIDSLAQQNNLKDALASLKSINAQ
HHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCHH
FRAKQAQIRQAKLEFSRQQQMLADKASSRADYEVADANLTVYQAELEQLEAQKQQAEINV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEHHHHHHHHHHHHHHEEEC
DSARIDLGYTKITAPMDGTVVYSAVEVGQTVNANQTTPTIVEMAQLDTMTVKAQISEADV
CCEEEEECEEEEECCCCCEEEEEEEHHCCCCCCCCCCCHHHHHHHHCEEEEEEEECCCCE
VNVHPGQAVYFTVLGKPNHPYRGILRAIEPGPISMDGDDSNMSSSDSDAIYYHGLFDVEN
EEECCCCEEEEEEECCCCCCHHHHHHHCCCCCCEECCCCCCCCCCCCCEEEEEEEEECCC
PDRTLRIGMTAQVSIVLAKADDALLVPAQILRIKPREKSAAPEAKSGERKARSGPQYQVP
CCCEEEEECEEEEEEEEEECCCCEECCHHHEEECCCCCCCCCCCCCCCHHHCCCCCEEEE
VLVDNQVQYVDVTVGINNKINAEITTGLKEGDQVVLGMPSADDGSSKFRRQPSVRF
EEECCCEEEEEEEEECCCEECEEEECCCCCCCEEEEECCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]