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The map label for this gene is cysJ [H]

Identifier: 126175613

GI number: 126175613

Start: 4003503

End: 4005302

Strand: Reverse

Name: cysJ [H]

Synonym: Sbal_3416

Alternate gene names: 126175613

Gene position: 4005302-4003503 (Counterclockwise)

Preceding gene: 126175617

Following gene: 126175612

Centisome position: 78.12

GC content: 49.61

Gene sequence:

>1800_bases
ATGTTGTTAAAAGAACTTTCATCGCTGGCTTCGCCATTGTCTCAAGGACAAGTCGAGAAGCTAAAACAACTCACTTCAGA
GTTAAGTGCTGTGCAACTGGCTTGGGTGAGTGGCTATCTTGCTGCCACCGCCAATGCTGGCCAACTAGCTCCCGCCGCAG
AGGCGCAAACTGCTCAAACAGTGACGATTCTCTATGGCAGCCAAACGGGTAATGGCCGTGGTGTTGCCAAAGCGCTCGCC
GATAAGGCGCAGGCGCAAGGTTATGCGGTCAATCTGGCTTCAATGGGCGAATACAATGTTCGCCAACTGAAACAAGAAAC
TGTGCTCTTGCTGGTGGTCAGCACCCATGGTGAAGGTGAAGCGCCGGATGACGCGATTGAATTACATAAGTTTTTAGCAT
CGAAACGTGCGCCTAAGTTAGACAATCTACATTACTCAGTGCTTGCGCTGGGGGATTCAAGCTATGAATTCTTCTGCCAA
ACCGGTAAAGACTTTAGTACTCGTCTAGCGCTACTGGGTGCTAAGTCATTGTTACCTTTGGTTGAATGTGATGTGGACTA
TGAAGCCGCCGCTGGTCAGTGGCACGCCGATGTGTTAGAGGCCGTTAAGCCGCTTATCGAAACCTCGTCCGCCAGTGTGG
TATCTATCGGTACAGCTAAAGCGATTGGTGAAAGCGAGTTTACTAAGCAAAACCCCTATAGCGCTGAAGTGCTAGCGAGC
CAAAAAATTACCGGCCGCGGTTCAGACCGAGATGTGCGCCATGTTGAAATCGACCTTGGCGATTCAGGCTTAAGCTATCA
AGCGGGCGATGCCCTCGGTGTGTGGTTTAGCAATAATGAAGTGTTAGTCGATGAAGTCTTAGTGGGTCTGTCATTGGCCG
CCGATGAGTTAGTAACCCTAGGTACAGAATCTTTGACCCTTAAGCAAGCTTTGCTTGAGAAGAAAGAGCTGACTCAGTTG
TATCCTGGATTAGTCAAAGCATGGGCCGAACTGAGCGGCAGCGCTGAACTGCTGGCATTGAGCGAAGATAAAGAGCAAGT
GCGTCACTTTATCCTTAAGCATCAATTTGCGGATTTAGTCGCCCAGTATCCGTTAACAAATAGCGCTGTCACGTTAAGCG
CGGCAAGATTGCTTGAGTTACTGCGCCCATTAACCCCAAGACTTTACTCTATCGCCTCAAGCCAAAGTGAAGTGGAAACC
GAAGTCCATTTAACCGTAGCGTTAGTGGAAGATGAGCGTCACGGCGCGGCCCGTTTTGGCGGCGCGTCACACTTCTTAGC
CTCTGCGCAGGAAGGCACTCAGGTTAAGGTTTATGTTGAGTCGAACAAGCATTTCCGTTTACCCGAGAATCCAGACACAC
CAGTTATTATGGTTGGCCCAGGCACAGGTGTTGCGCCGTTTCGTGCCTTTATGCAGGAGCGCGTGGCCCAAGGGATCCAA
GGTGATAGCTGGTTATTCTTCGGCAATCCACACTTTGAGCAGGATTTCTTGTATCAAACTGAGTGGCAACAGTATTTGAA
AAATGGCGATTTAAGCCGTATCGACGTGGCGTTCTCGCGCGATCAAGCCCATAAAGTTTATGTGCAGCACCGTATTAAAG
AACAAGGCCAAGCCCTGTGGCAATGGTTGCAAAACGGCGCGCATCTGTACATTTGTGGTGATGCTGAACGTATGGCAAAA
GATGTCCACCAAGCCTTGATAGAGGTGGCTGTCGAAGTCGGTGGTTTGGATACCGAAGCTGCAGAAGCCTATTTTGAAAC
ACTGCGCAGCGATAAGCGTTACCAAAAAGACGTGTATTAA

Upstream 100 bases:

>100_bases
TGTATATTCCAAAATGGAATTAAAAATCCGATTTTATTCTTTTTTTCTTTTCTTAAACCGCGATAAGCTTGTGGACATTA
CACTGTCATAGGGCCGTCTC

Downstream 100 bases:

>100_bases
TCTCAACCGTTACGCATATAAGAAACGCATAAGAATAGCCAAAGCTGCACACGTGATGACCTGTGCAGTCAGCGAACCGA
ATTTGAAGAGGCAGTTGCCA

Product: sulfite reductase (NADPH) flavoprotein, alpha chain

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 599; Mature: 599

Protein sequence:

>599_residues
MLLKELSSLASPLSQGQVEKLKQLTSELSAVQLAWVSGYLAATANAGQLAPAAEAQTAQTVTILYGSQTGNGRGVAKALA
DKAQAQGYAVNLASMGEYNVRQLKQETVLLLVVSTHGEGEAPDDAIELHKFLASKRAPKLDNLHYSVLALGDSSYEFFCQ
TGKDFSTRLALLGAKSLLPLVECDVDYEAAAGQWHADVLEAVKPLIETSSASVVSIGTAKAIGESEFTKQNPYSAEVLAS
QKITGRGSDRDVRHVEIDLGDSGLSYQAGDALGVWFSNNEVLVDEVLVGLSLAADELVTLGTESLTLKQALLEKKELTQL
YPGLVKAWAELSGSAELLALSEDKEQVRHFILKHQFADLVAQYPLTNSAVTLSAARLLELLRPLTPRLYSIASSQSEVET
EVHLTVALVEDERHGAARFGGASHFLASAQEGTQVKVYVESNKHFRLPENPDTPVIMVGPGTGVAPFRAFMQERVAQGIQ
GDSWLFFGNPHFEQDFLYQTEWQQYLKNGDLSRIDVAFSRDQAHKVYVQHRIKEQGQALWQWLQNGAHLYICGDAERMAK
DVHQALIEVAVEVGGLDTEAAEAYFETLRSDKRYQKDVY

Sequences:

>Translated_599_residues
MLLKELSSLASPLSQGQVEKLKQLTSELSAVQLAWVSGYLAATANAGQLAPAAEAQTAQTVTILYGSQTGNGRGVAKALA
DKAQAQGYAVNLASMGEYNVRQLKQETVLLLVVSTHGEGEAPDDAIELHKFLASKRAPKLDNLHYSVLALGDSSYEFFCQ
TGKDFSTRLALLGAKSLLPLVECDVDYEAAAGQWHADVLEAVKPLIETSSASVVSIGTAKAIGESEFTKQNPYSAEVLAS
QKITGRGSDRDVRHVEIDLGDSGLSYQAGDALGVWFSNNEVLVDEVLVGLSLAADELVTLGTESLTLKQALLEKKELTQL
YPGLVKAWAELSGSAELLALSEDKEQVRHFILKHQFADLVAQYPLTNSAVTLSAARLLELLRPLTPRLYSIASSQSEVET
EVHLTVALVEDERHGAARFGGASHFLASAQEGTQVKVYVESNKHFRLPENPDTPVIMVGPGTGVAPFRAFMQERVAQGIQ
GDSWLFFGNPHFEQDFLYQTEWQQYLKNGDLSRIDVAFSRDQAHKVYVQHRIKEQGQALWQWLQNGAHLYICGDAERMAK
DVHQALIEVAVEVGGLDTEAAEAYFETLRSDKRYQKDVY
>Mature_599_residues
MLLKELSSLASPLSQGQVEKLKQLTSELSAVQLAWVSGYLAATANAGQLAPAAEAQTAQTVTILYGSQTGNGRGVAKALA
DKAQAQGYAVNLASMGEYNVRQLKQETVLLLVVSTHGEGEAPDDAIELHKFLASKRAPKLDNLHYSVLALGDSSYEFFCQ
TGKDFSTRLALLGAKSLLPLVECDVDYEAAAGQWHADVLEAVKPLIETSSASVVSIGTAKAIGESEFTKQNPYSAEVLAS
QKITGRGSDRDVRHVEIDLGDSGLSYQAGDALGVWFSNNEVLVDEVLVGLSLAADELVTLGTESLTLKQALLEKKELTQL
YPGLVKAWAELSGSAELLALSEDKEQVRHFILKHQFADLVAQYPLTNSAVTLSAARLLELLRPLTPRLYSIASSQSEVET
EVHLTVALVEDERHGAARFGGASHFLASAQEGTQVKVYVESNKHFRLPENPDTPVIMVGPGTGVAPFRAFMQERVAQGIQ
GDSWLFFGNPHFEQDFLYQTEWQQYLKNGDLSRIDVAFSRDQAHKVYVQHRIKEQGQALWQWLQNGAHLYICGDAERMAK
DVHQALIEVAVEVGGLDTEAAEAYFETLRSDKRYQKDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI7657393, Length=592, Percent_Identity=31.7567567567568, Blast_Score=256, Evalue=6e-68,
Organism=Homo sapiens, GI221316709, Length=592, Percent_Identity=31.7567567567568, Blast_Score=251, Evalue=2e-66,
Organism=Homo sapiens, GI221316705, Length=601, Percent_Identity=31.2811980033278, Blast_Score=248, Evalue=9e-66,
Organism=Homo sapiens, GI127139033, Length=617, Percent_Identity=30.4700162074554, Blast_Score=243, Evalue=4e-64,
Organism=Homo sapiens, GI221316707, Length=520, Percent_Identity=32.1153846153846, Blast_Score=225, Evalue=1e-58,
Organism=Homo sapiens, GI24041029, Length=600, Percent_Identity=29.8333333333333, Blast_Score=205, Evalue=1e-52,
Organism=Homo sapiens, GI10835173, Length=650, Percent_Identity=27.0769230769231, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI40254422, Length=647, Percent_Identity=27.2024729520866, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI169790956, Length=410, Percent_Identity=30.4878048780488, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI169790958, Length=410, Percent_Identity=30.4878048780488, Blast_Score=157, Evalue=3e-38,
Organism=Escherichia coli, GI1789123, Length=593, Percent_Identity=51.602023608769, Blast_Score=603, Evalue=1e-174,
Organism=Caenorhabditis elegans, GI17554134, Length=596, Percent_Identity=29.3624161073826, Blast_Score=222, Evalue=6e-58,
Organism=Caenorhabditis elegans, GI17566446, Length=579, Percent_Identity=28.3246977547496, Blast_Score=170, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17531441, Length=416, Percent_Identity=25.9615384615385, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321832, Length=648, Percent_Identity=30.5555555555556, Blast_Score=217, Evalue=5e-57,
Organism=Saccharomyces cerevisiae, GI6321143, Length=394, Percent_Identity=31.7258883248731, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6325305, Length=632, Percent_Identity=23.4177215189873, Blast_Score=139, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6322597, Length=160, Percent_Identity=30.625, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17137192, Length=615, Percent_Identity=29.4308943089431, Blast_Score=222, Evalue=6e-58,
Organism=Drosophila melanogaster, GI24582192, Length=524, Percent_Identity=29.7709923664122, Blast_Score=189, Evalue=6e-48,
Organism=Drosophila melanogaster, GI24660907, Length=596, Percent_Identity=28.0201342281879, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24660903, Length=596, Percent_Identity=28.0201342281879, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI78706872, Length=535, Percent_Identity=27.8504672897196, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24583543, Length=535, Percent_Identity=27.8504672897196, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI78706876, Length=535, Percent_Identity=27.8504672897196, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24647438, Length=236, Percent_Identity=27.5423728813559, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 65394; Mature: 65394

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKELSSLASPLSQGQVEKLKQLTSELSAVQLAWVSGYLAATANAGQLAPAAEAQTAQT
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
VTILYGSQTGNGRGVAKALADKAQAQGYAVNLASMGEYNVRQLKQETVLLLVVSTHGEGE
EEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEHHCCCHHHHHHCCCEEEEEEEECCCCCC
APDDAIELHKFLASKRAPKLDNLHYSVLALGDSSYEFFCQTGKDFSTRLALLGAKSLLPL
CCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHCCHH
VECDVDYEAAAGQWHADVLEAVKPLIETSSASVVSIGTAKAIGESEFTKQNPYSAEVLAS
HCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHCCCCCCCCCCCCCHHHHHC
QKITGRGSDRDVRHVEIDLGDSGLSYQAGDALGVWFSNNEVLVDEVLVGLSLAADELVTL
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHC
GTESLTLKQALLEKKELTQLYPGLVKAWAELSGSAELLALSEDKEQVRHFILKHQFADLV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHH
AQYPLTNSAVTLSAARLLELLRPLTPRLYSIASSQSEVETEVHLTVALVEDERHGAARFG
HHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCHHHCCEEEEEEEEEEECCCCCCHHCC
GASHFLASAQEGTQVKVYVESNKHFRLPENPDTPVIMVGPGTGVAPFRAFMQERVAQGIQ
CHHHHHHCCCCCCEEEEEEECCCEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCC
GDSWLFFGNPHFEQDFLYQTEWQQYLKNGDLSRIDVAFSRDQAHKVYVQHRIKEQGQALW
CCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
QWLQNGAHLYICGDAERMAKDVHQALIEVAVEVGGLDTEAAEAYFETLRSDKRYQKDVY
HHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure
MLLKELSSLASPLSQGQVEKLKQLTSELSAVQLAWVSGYLAATANAGQLAPAAEAQTAQT
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
VTILYGSQTGNGRGVAKALADKAQAQGYAVNLASMGEYNVRQLKQETVLLLVVSTHGEGE
EEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEHHCCCHHHHHHCCCEEEEEEEECCCCCC
APDDAIELHKFLASKRAPKLDNLHYSVLALGDSSYEFFCQTGKDFSTRLALLGAKSLLPL
CCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHCCHH
VECDVDYEAAAGQWHADVLEAVKPLIETSSASVVSIGTAKAIGESEFTKQNPYSAEVLAS
HCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHCCCCCCCCCCCCCHHHHHC
QKITGRGSDRDVRHVEIDLGDSGLSYQAGDALGVWFSNNEVLVDEVLVGLSLAADELVTL
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHC
GTESLTLKQALLEKKELTQLYPGLVKAWAELSGSAELLALSEDKEQVRHFILKHQFADLV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHH
AQYPLTNSAVTLSAARLLELLRPLTPRLYSIASSQSEVETEVHLTVALVEDERHGAARFG
HHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCHHHCCEEEEEEEEEEECCCCCCHHCC
GASHFLASAQEGTQVKVYVESNKHFRLPENPDTPVIMVGPGTGVAPFRAFMQERVAQGIQ
CHHHHHHCCCCCCEEEEEEECCCEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCC
GDSWLFFGNPHFEQDFLYQTEWQQYLKNGDLSRIDVAFSRDQAHKVYVQHRIKEQGQALW
CCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
QWLQNGAHLYICGDAERMAKDVHQALIEVAVEVGGLDTEAAEAYFETLRSDKRYQKDVY
HHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]