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The map label for this gene is carA [H]

Identifier: 126175505

GI number: 126175505

Start: 3870960

End: 3872120

Strand: Reverse

Name: carA [H]

Synonym: Sbal_3305

Alternate gene names: 126175505

Gene position: 3872120-3870960 (Counterclockwise)

Preceding gene: 126175506

Following gene: 126175504

Centisome position: 75.52

GC content: 48.49

Gene sequence:

>1161_bases
ATGGAGGTCGCGTTGACAAAGTCTGCCTTACTCGTACTCGAAGATGGAACTGTATTCTCTGGCACAGCAATTGGTGCCGA
TGGTCTCTCTGTTGGTGAAGTTGTTTTTAACACTTCCATGACAGGTTACCAAGAAATTTTAACTGATCCATCCTACTCAC
GCCAGATTGTAACATTAACCTACCCACACATAGGCAACACTGGTACCAATGATGAAGATGTTGAATCCGATGGGGTTCAC
GCTTGTGGTCTTATCATTCGTGATCTTCCGCTCATCGCAAGTAGTTTCCGCAATCAACAAAGCTTAAGTGATTATCTCAA
AGCGAACAATGTCGTTGGCATAGCAGATATCGATACCCGCAAGTTAACTCGTATTCTCCGTGAAAAAGGTGCACAAGCCG
GTTGTATTATGGCAGGTGCAGACTTAGACGAAGCAAAAGCGCTTGCCGCTGCCAAAGCCTTCCCCGGTTTAAAAGGCATG
GATCTCGCTAAAGAAGTCACAACTAAAGCGGCCTATCCATGGCGCAAAGGTAGCTGGCGTTTAGTGGGCGGTTTACCTGC
AGATACGCCAGCAAATGAGCTTAAATACAAAGTAGTAGCCTACGACTACGGCGTTAAGCAAAACATTCTGCGTATGTTAG
TGGACCGCGGTTGCGACGTCACTGTTGTGCCAGCTAAAACCTCTGCCGCCGACGTGCTGGCGATGAATCCCGATGGTGTG
TTCCTTTCGAACGGCCCTGGCGATCCAGAACCATGCGATTACGCAATTGAAGCGATTCAACAGATCCTGAAAACTGACAC
ACCGATTTTTGGTATCTGTTTAGGTCATCAGTTGTTAGCTCTGGCCTCAGGTGCAAAAACCTTAAAGATGAAGTTTGGTC
ACCACGGTGCAAACCACCCAGTGAGCAAGATCGAGAAAGGTAACGTGATGATCACCAGCCAAAACCACGGTTTTGCTGCC
GATGAAAGCACGCTACCCGCCAACATTAAGGTGACGCATAAGTCGCTGTTTGATGGTTCACTGCAAGGTATCCATCTTAC
GGACAAACCTGCGTTCAGCTTCCAAGGTCACCCTGAAGCGAGCCCAGGCCCGAACGATGCCGCCCCGCTGTTCGATCACT
TTATCGAGCTAATCGACATATACCGTCAGAACGCCAAGTAG

Upstream 100 bases:

>100_bases
TCCTGTAAACTCGCTGGAATTTGTCAAAATTTCGTAAATTAGCGGAAATTGGTATCTTAACATAACATAAAAACATTATA
TTTCAGTGGCTTGGCTCTTT

Downstream 100 bases:

>100_bases
TACGCTAGCTAGGAGAAGAGAGAAGCAATGCCAAAACGTACAGATATAAAGAGTATTCTTATCCTAGGCGCCGGCCCAAT
CGTGATTGGTCAGGCATGTG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MEVALTKSALLVLEDGTVFSGTAIGADGLSVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNTGTNDEDVESDGVH
ACGLIIRDLPLIASSFRNQQSLSDYLKANNVVGIADIDTRKLTRILREKGAQAGCIMAGADLDEAKALAAAKAFPGLKGM
DLAKEVTTKAAYPWRKGSWRLVGGLPADTPANELKYKVVAYDYGVKQNILRMLVDRGCDVTVVPAKTSAADVLAMNPDGV
FLSNGPGDPEPCDYAIEAIQQILKTDTPIFGICLGHQLLALASGAKTLKMKFGHHGANHPVSKIEKGNVMITSQNHGFAA
DESTLPANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEASPGPNDAAPLFDHFIELIDIYRQNAK

Sequences:

>Translated_386_residues
MEVALTKSALLVLEDGTVFSGTAIGADGLSVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNTGTNDEDVESDGVH
ACGLIIRDLPLIASSFRNQQSLSDYLKANNVVGIADIDTRKLTRILREKGAQAGCIMAGADLDEAKALAAAKAFPGLKGM
DLAKEVTTKAAYPWRKGSWRLVGGLPADTPANELKYKVVAYDYGVKQNILRMLVDRGCDVTVVPAKTSAADVLAMNPDGV
FLSNGPGDPEPCDYAIEAIQQILKTDTPIFGICLGHQLLALASGAKTLKMKFGHHGANHPVSKIEKGNVMITSQNHGFAA
DESTLPANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEASPGPNDAAPLFDHFIELIDIYRQNAK
>Mature_386_residues
MEVALTKSALLVLEDGTVFSGTAIGADGLSVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNTGTNDEDVESDGVH
ACGLIIRDLPLIASSFRNQQSLSDYLKANNVVGIADIDTRKLTRILREKGAQAGCIMAGADLDEAKALAAAKAFPGLKGM
DLAKEVTTKAAYPWRKGSWRLVGGLPADTPANELKYKVVAYDYGVKQNILRMLVDRGCDVTVVPAKTSAADVLAMNPDGV
FLSNGPGDPEPCDYAIEAIQQILKTDTPIFGICLGHQLLALASGAKTLKMKFGHHGANHPVSKIEKGNVMITSQNHGFAA
DESTLPANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEASPGPNDAAPLFDHFIELIDIYRQNAK

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=381, Percent_Identity=38.8451443569554, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI21361331, Length=385, Percent_Identity=37.1428571428571, Blast_Score=225, Evalue=4e-59,
Organism=Homo sapiens, GI169790915, Length=385, Percent_Identity=37.1428571428571, Blast_Score=225, Evalue=6e-59,
Organism=Escherichia coli, GI1786215, Length=382, Percent_Identity=76.9633507853403, Blast_Score=619, Evalue=1e-178,
Organism=Caenorhabditis elegans, GI193204318, Length=382, Percent_Identity=37.434554973822, Blast_Score=230, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6324878, Length=401, Percent_Identity=39.9002493765586, Blast_Score=246, Evalue=4e-66,
Organism=Saccharomyces cerevisiae, GI6322331, Length=406, Percent_Identity=36.2068965517241, Blast_Score=236, Evalue=4e-63,
Organism=Drosophila melanogaster, GI45555749, Length=387, Percent_Identity=39.7932816537468, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24642586, Length=387, Percent_Identity=39.7932816537468, Blast_Score=233, Evalue=1e-61,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 41231; Mature: 41231

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVALTKSALLVLEDGTVFSGTAIGADGLSVGEVVFNTSMTGYQEILTDPSYSRQIVTLT
CCEEEECCEEEEEECCCEEECCEECCCCCCHHHHEECCCCCHHHHHHCCCCCCCEEEEEE
YPHIGNTGTNDEDVESDGVHACGLIIRDLPLIASSFRNQQSLSDYLKANNVVGIADIDTR
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCEEEEECCCHH
KLTRILREKGAQAGCIMAGADLDEAKALAAAKAFPGLKGMDLAKEVTTKAAYPWRKGSWR
HHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEE
LVGGLPADTPANELKYKVVAYDYGVKQNILRMLVDRGCDVTVVPAKTSAADVLAMNPDGV
EEECCCCCCCHHHEEEEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCE
FLSNGPGDPEPCDYAIEAIQQILKTDTPIFGICLGHQLLALASGAKTLKMKFGHHGANHP
EECCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCEEEEEECCCCCCCC
VSKIEKGNVMITSQNHGFAADESTLPANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEA
HHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEEHHHCCCCCCEEEECCCCCCCCCCCCCC
SPGPNDAAPLFDHFIELIDIYRQNAK
CCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEVALTKSALLVLEDGTVFSGTAIGADGLSVGEVVFNTSMTGYQEILTDPSYSRQIVTLT
CCEEEECCEEEEEECCCEEECCEECCCCCCHHHHEECCCCCHHHHHHCCCCCCCEEEEEE
YPHIGNTGTNDEDVESDGVHACGLIIRDLPLIASSFRNQQSLSDYLKANNVVGIADIDTR
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCEEEEECCCHH
KLTRILREKGAQAGCIMAGADLDEAKALAAAKAFPGLKGMDLAKEVTTKAAYPWRKGSWR
HHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEE
LVGGLPADTPANELKYKVVAYDYGVKQNILRMLVDRGCDVTVVPAKTSAADVLAMNPDGV
EEECCCCCCCHHHEEEEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCE
FLSNGPGDPEPCDYAIEAIQQILKTDTPIFGICLGHQLLALASGAKTLKMKFGHHGANHP
EECCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCEEEEEECCCCCCCC
VSKIEKGNVMITSQNHGFAADESTLPANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEA
HHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEEHHHCCCCCCEEEECCCCCCCCCCCCCC
SPGPNDAAPLFDHFIELIDIYRQNAK
CCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]