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The map label for this gene is mltB [H]

Identifier: 126175477

GI number: 126175477

Start: 3839542

End: 3840612

Strand: Direct

Name: mltB [H]

Synonym: Sbal_3277

Alternate gene names: 126175477

Gene position: 3839542-3840612 (Clockwise)

Preceding gene: 126175476

Following gene: 126175478

Centisome position: 74.88

GC content: 46.87

Gene sequence:

>1071_bases
ATGCCTACTCTCCGAGCTTTTTTAGCTCCACTCGGTTTACTGTGTTTTAGTTCATTGTTAGTCGCCTGTACCACGGCGAA
CGAACCGACACCCGCGCAGGCACCCGTTCCAAGCACGCCAGTGGCTCCGGCCGCTGAAGTCGCGCCCACAATTCCCGCTG
AACTCAAAGAAGAGTTTATCCAAGCTCAAGTAAAACAAGGTTTTACTCGTGCAGAAGTGCTCGCTTTTCTCGACAAGGCA
AAATACAACCAAGGGGTTATCGATGCGATGACTCGTCCATGGGAAGCAAAACCTTGGCATGTGTATTATCCCATCTTCTT
AACCGAGAAACGCCTCGACGCTGGTCTCGCCTTTTGGAAAAAACACGAGAAAACCATCGCGAAAGCCGCGGCTAAGTACA
AAGTTGACCCACAGATTATTGTCGCCATTATCGGCATTGAAACCTTCTACGGCCAATACACGGGTAATTATCCTGTGATT
GATGCCCTCTATACCCTAGGTTTTCACTATCCGCCGCGCGCCACCTTTTTCCGTAAGGAATTTGGCGAGTTGATGAAGTT
AGTGAAAGAAGAACATTTAGATATAGATACCCTCAAGGGCTCCTACGCAGGCGCTATGGGTTATGGCCAGTTTATCCCCT
CAAGTTACCGCCATTACGCGGTTGATTTTGATGGCAGCGGCAACCGCGATTTATTACAAAGTCCTGAGGATGCCATCGGC
AGTGTGGCCAATTATTTCTACAAACACGGTTGGCAAGCCAATGCGCCGGTCGCTTTACCGTTAGTGAATAACAGCAAAAC
TGCACCTAAAGCGAAAGTGTGGGCAGGTGAAAAGCTGGACTATAAAGTTGCAGATATTTTAACGCCGACCTTAGCCTTAG
CCGATGCGCGGGACTTAGATGTTTCGCAAAAAGCCTTACTGATTGAGCTTGAACAAGCGGATCACAGTGATTACTGGCTT
GGCTTAAATAACTTTTATGTGATCACTCGTTATAATCGTAGCCCTTTGTATTCAATGGCCGTTTATCAATTTAGCCAACA
ATTAAAAGAACAGCATGCCTCTAAAAAATAA

Upstream 100 bases:

>100_bases
GGTGGAACCTCCATGCTGACATTGATGACAGGGTTTGGCATTCTTATGAGCATTCATACCCATAGACGTTTCGTAGATAG
ATAATTTTTAGGGACTTTTC

Downstream 100 bases:

>100_bases
TATTTTACAGCTTGCCGCCTTGGTTATGTGCTTCGCCCTTGCGGCGTGTTCTTCAAGCCCAAACAACGCCAGCAAGAAAA
ACATGGACCCGAACAAGGGC

Product: lytic murein transglycosylase B

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MPTLRAFLAPLGLLCFSSLLVACTTANEPTPAQAPVPSTPVAPAAEVAPTIPAELKEEFIQAQVKQGFTRAEVLAFLDKA
KYNQGVIDAMTRPWEAKPWHVYYPIFLTEKRLDAGLAFWKKHEKTIAKAAAKYKVDPQIIVAIIGIETFYGQYTGNYPVI
DALYTLGFHYPPRATFFRKEFGELMKLVKEEHLDIDTLKGSYAGAMGYGQFIPSSYRHYAVDFDGSGNRDLLQSPEDAIG
SVANYFYKHGWQANAPVALPLVNNSKTAPKAKVWAGEKLDYKVADILTPTLALADARDLDVSQKALLIELEQADHSDYWL
GLNNFYVITRYNRSPLYSMAVYQFSQQLKEQHASKK

Sequences:

>Translated_356_residues
MPTLRAFLAPLGLLCFSSLLVACTTANEPTPAQAPVPSTPVAPAAEVAPTIPAELKEEFIQAQVKQGFTRAEVLAFLDKA
KYNQGVIDAMTRPWEAKPWHVYYPIFLTEKRLDAGLAFWKKHEKTIAKAAAKYKVDPQIIVAIIGIETFYGQYTGNYPVI
DALYTLGFHYPPRATFFRKEFGELMKLVKEEHLDIDTLKGSYAGAMGYGQFIPSSYRHYAVDFDGSGNRDLLQSPEDAIG
SVANYFYKHGWQANAPVALPLVNNSKTAPKAKVWAGEKLDYKVADILTPTLALADARDLDVSQKALLIELEQADHSDYWL
GLNNFYVITRYNRSPLYSMAVYQFSQQLKEQHASKK
>Mature_355_residues
PTLRAFLAPLGLLCFSSLLVACTTANEPTPAQAPVPSTPVAPAAEVAPTIPAELKEEFIQAQVKQGFTRAEVLAFLDKAK
YNQGVIDAMTRPWEAKPWHVYYPIFLTEKRLDAGLAFWKKHEKTIAKAAAKYKVDPQIIVAIIGIETFYGQYTGNYPVID
ALYTLGFHYPPRATFFRKEFGELMKLVKEEHLDIDTLKGSYAGAMGYGQFIPSSYRHYAVDFDGSGNRDLLQSPEDAIGS
VANYFYKHGWQANAPVALPLVNNSKTAPKAKVWAGEKLDYKVADILTPTLALADARDLDVSQKALLIELEQADHSDYWLG
LNNFYVITRYNRSPLYSMAVYQFSQQLKEQHASKK

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=305, Percent_Identity=40, Blast_Score=200, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 39798; Mature: 39667

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTLRAFLAPLGLLCFSSLLVACTTANEPTPAQAPVPSTPVAPAAEVAPTIPAELKEEFI
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHH
QAQVKQGFTRAEVLAFLDKAKYNQGVIDAMTRPWEAKPWHVYYPIFLTEKRLDAGLAFWK
HHHHHCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEEEEEECHHHHHHHHHHH
KHEKTIAKAAAKYKVDPQIIVAIIGIETFYGQYTGNYPVIDALYTLGFHYPPRATFFRKE
HHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHH
FGELMKLVKEEHLDIDTLKGSYAGAMGYGQFIPSSYRHYAVDFDGSGNRDLLQSPEDAIG
HHHHHHHHHHHCCCHHHCCCCCCCCCCHHHCCCCCCCEEEEEECCCCCCHHHCCCHHHHH
SVANYFYKHGWQANAPVALPLVNNSKTAPKAKVWAGEKLDYKVADILTPTLALADARDLD
HHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCC
VSQKALLIELEQADHSDYWLGLNNFYVITRYNRSPLYSMAVYQFSQQLKEQHASKK
CCCCEEEEEEECCCCCCEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PTLRAFLAPLGLLCFSSLLVACTTANEPTPAQAPVPSTPVAPAAEVAPTIPAELKEEFI
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHH
QAQVKQGFTRAEVLAFLDKAKYNQGVIDAMTRPWEAKPWHVYYPIFLTEKRLDAGLAFWK
HHHHHCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEEEEEECHHHHHHHHHHH
KHEKTIAKAAAKYKVDPQIIVAIIGIETFYGQYTGNYPVIDALYTLGFHYPPRATFFRKE
HHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHH
FGELMKLVKEEHLDIDTLKGSYAGAMGYGQFIPSSYRHYAVDFDGSGNRDLLQSPEDAIG
HHHHHHHHHHHCCCHHHCCCCCCCCCCHHHCCCCCCCEEEEEECCCCCCHHHCCCHHHHH
SVANYFYKHGWQANAPVALPLVNNSKTAPKAKVWAGEKLDYKVADILTPTLALADARDLD
HHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCC
VSQKALLIELEQADHSDYWLGLNNFYVITRYNRSPLYSMAVYQFSQQLKEQHASKK
CCCCEEEEEEECCCCCCEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]