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The map label for this gene is rrmJ

Identifier: 126175447

GI number: 126175447

Start: 3808057

End: 3808686

Strand: Reverse

Name: rrmJ

Synonym: Sbal_3247

Alternate gene names: 126175447

Gene position: 3808686-3808057 (Counterclockwise)

Preceding gene: 126175449

Following gene: 126175446

Centisome position: 74.28

GC content: 46.98

Gene sequence:

>630_bases
ATGTCAGGTATAAAACGTACGGCCAGTTCTAATCGCTGGATGCTAGAACACTTTGATGATCACTATGTCAAACTCGCCCA
AAAAATGGGCTTGCGATCTCGCGCTGCGTTTAAGCTTGAGGAGATCCAGCAAAAGGATCAACTGATTCGCCCAGGCATGA
CTGTGGTTGATCTCGGTGCTGCTCCAGGAGGTTGGTCTCAAGTGGCAGTTAAATTAGCGGGAGATAGAGGTAAAGTCATC
GCCTGTGACATTTTGCCTATGGATCCCATCGTCGGGGTAGATTTTTTGCAAGGGGATTTTCGTGAAGATAAAGTCCTTCA
AGCATTACTTACTCGCGTCGGTGATGCTAAGGTTGACGTTGTGTTGTCTGATATGGCGCCCAACATGAGTGGTTCAGATA
GTGTTGATCAACCCCGTGCCATGTATTTAGTGGAGTTGGCGTTAGATATGTGCCATCAAGTATTGGCTCCTAATGGCTGT
TTTGCGGTAAAAGTCTTTCAGGGGGAGGGCTTTGACGAATACATGAAAGCGGTAAAAGCAGTGTTTAAAGTCGTTAAAAC
ACGTAAACCAGACTCTTCACGGGCGCGTTCCCGTGAAGTCTATCTTGTGGCGACTGGGTACAAGTTGTAG

Upstream 100 bases:

>100_bases
TTAAGTTCATTTCTACCAACTTTTGCTGAGTTACTGTTGAAAAACGGCTATTCTACCCTTATATAGCCTTTGTGTAACTC
TCAATTGTTGCGGATTTTAA

Downstream 100 bases:

>100_bases
TAACCTAGCGTTAATAATCGCAAGACTTAATGAGGTCTAGTAATTTGAGTGACATGGCAAAAAATCTAATACTCTGGGTT
GTCATCGCCGTTGTGCTGAT

Product: 23S rRNA methyltransferase J

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MSGIKRTASSNRWMLEHFDDHYVKLAQKMGLRSRAAFKLEEIQQKDQLIRPGMTVVDLGAAPGGWSQVAVKLAGDRGKVI
ACDILPMDPIVGVDFLQGDFREDKVLQALLTRVGDAKVDVVLSDMAPNMSGSDSVDQPRAMYLVELALDMCHQVLAPNGC
FAVKVFQGEGFDEYMKAVKAVFKVVKTRKPDSSRARSREVYLVATGYKL

Sequences:

>Translated_209_residues
MSGIKRTASSNRWMLEHFDDHYVKLAQKMGLRSRAAFKLEEIQQKDQLIRPGMTVVDLGAAPGGWSQVAVKLAGDRGKVI
ACDILPMDPIVGVDFLQGDFREDKVLQALLTRVGDAKVDVVLSDMAPNMSGSDSVDQPRAMYLVELALDMCHQVLAPNGC
FAVKVFQGEGFDEYMKAVKAVFKVVKTRKPDSSRARSREVYLVATGYKL
>Mature_208_residues
SGIKRTASSNRWMLEHFDDHYVKLAQKMGLRSRAAFKLEEIQQKDQLIRPGMTVVDLGAAPGGWSQVAVKLAGDRGKVIA
CDILPMDPIVGVDFLQGDFREDKVLQALLTRVGDAKVDVVLSDMAPNMSGSDSVDQPRAMYLVELALDMCHQVLAPNGCF
AVKVFQGEGFDEYMKAVKAVFKVVKTRKPDSSRARSREVYLVATGYKL

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=212, Percent_Identity=35.8490566037736, Blast_Score=138, Evalue=4e-33,
Organism=Homo sapiens, GI194097365, Length=188, Percent_Identity=35.1063829787234, Blast_Score=120, Evalue=9e-28,
Organism=Homo sapiens, GI29029591, Length=190, Percent_Identity=35.7894736842105, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI29029589, Length=190, Percent_Identity=35.7894736842105, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI7110661, Length=190, Percent_Identity=35.7894736842105, Blast_Score=115, Evalue=3e-26,
Organism=Escherichia coli, GI1789569, Length=208, Percent_Identity=72.1153846153846, Blast_Score=318, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI17553474, Length=220, Percent_Identity=32.2727272727273, Blast_Score=110, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI71987550, Length=189, Percent_Identity=33.8624338624339, Blast_Score=107, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71987561, Length=189, Percent_Identity=33.8624338624339, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17553860, Length=189, Percent_Identity=33.8624338624339, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17554650, Length=191, Percent_Identity=34.5549738219895, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319535, Length=201, Percent_Identity=35.8208955223881, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6319796, Length=190, Percent_Identity=33.1578947368421, Blast_Score=102, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321302, Length=279, Percent_Identity=28.673835125448, Blast_Score=100, Evalue=2e-22,
Organism=Drosophila melanogaster, GI18859957, Length=188, Percent_Identity=37.2340425531915, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21356387, Length=219, Percent_Identity=35.6164383561644, Blast_Score=117, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24648639, Length=198, Percent_Identity=32.8282828282828, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24647580, Length=200, Percent_Identity=32, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_SHEB2 (B8E6M4)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002357058.1
- ProteinModelPortal:   B8E6M4
- SMR:   B8E6M4
- GeneID:   7087573
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_1120
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- ProtClustDB:   PRK11188
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 23096; Mature: 22965

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: ACT_SITE 164-164 BINDING 63-63 BINDING 65-65 BINDING 83-83 BINDING 99-99 BINDING 124-124

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGIKRTASSNRWMLEHFDDHYVKLAQKMGLRSRAAFKLEEIQQKDQLIRPGMTVVDLGA
CCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEECCC
APGGWSQVAVKLAGDRGKVIACDILPMDPIVGVDFLQGDFREDKVLQALLTRVGDAKVDV
CCCCHHHEEEEEECCCCCEEEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCEEEE
VLSDMAPNMSGSDSVDQPRAMYLVELALDMCHQVLAPNGCFAVKVFQGEGFDEYMKAVKA
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHH
VFKVVKTRKPDSSRARSREVYLVATGYKL
HHHHHHCCCCCCCCCCCCEEEEEECCCCC
>Mature Secondary Structure 
SGIKRTASSNRWMLEHFDDHYVKLAQKMGLRSRAAFKLEEIQQKDQLIRPGMTVVDLGA
CCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEECCC
APGGWSQVAVKLAGDRGKVIACDILPMDPIVGVDFLQGDFREDKVLQALLTRVGDAKVDV
CCCCHHHEEEEEECCCCCEEEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCEEEE
VLSDMAPNMSGSDSVDQPRAMYLVELALDMCHQVLAPNGCFAVKVFQGEGFDEYMKAVKA
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHH
VFKVVKTRKPDSSRARSREVYLVATGYKL
HHHHHHCCCCCCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA