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The map label for this gene is rfbB [H]

Identifier: 126175093

GI number: 126175093

Start: 3401676

End: 3402746

Strand: Reverse

Name: rfbB [H]

Synonym: Sbal_2890

Alternate gene names: 126175093

Gene position: 3402746-3401676 (Counterclockwise)

Preceding gene: 126175094

Following gene: 126175092

Centisome position: 66.36

GC content: 43.7

Gene sequence:

>1071_bases
GTGAAAATTCTGGTTACAGGTGGTGCTGGCTTTATCGGCTCTGCAGTCGTTCGTTTTATCATCAACAATACTCAAGATAG
CGTAATTAATGTCGATAAACTGACTTATGCTGGCAATCTTGAATCACTACTTAGTGTTGAAAAAAATCAACGATACGCAT
TCGAACAAGTGGATATTTGTGATCGAGTAGAGCTAGATAGAGTTTTTAATAAGTACCAACCTGATGCTGTGATGCATTTG
GCGGCTGAGTCCCATGTTGATCGCTCTATCACTGGCCCGGGCGATTTTATCCAAACCAATATTGTGGGTACTTATACGCT
ACTTGAAGCGGCCCGTCACTATTGGATGCAACTTGATGCTGAGCGTAAAAGCGCATTTCGTTTTCATCACATTTCAACCG
ATGAAGTTTATGGCGATTTACCTCATCCAGATGAGGTTGTGCCAGGCTCGGAATTAGCGCTATTCACTGAAATAACGCCT
TATGCGCCTAGCAGCCCATATTCAGCTTCTAAAGCCTCAAGTGACCATCTAGTGCGGGCATGGCTGCGCACCTATGGATT
ACCGACCATAGTCACAAACTGCTCCAACAATTATGGTCCGTATCACTTTCCTGAAAAGCTAATCCCGCTGGTGATCCTTA
ATGCACTCGAAGGCAAGGCATTGCCTATTTACGGTAAAGGCGACCAGATCCGCGATTGGTTATATGTCGAAGACCATGCT
CGCGCACTATACAAAGTAGTTACTGAGGGCAAAATTGGTGAAACCTACAATATTGGTGGTCATAACGAAAAACAGAACCT
AGAAGTGGTGCAAACCATCTGTAGCATCTTAGATTCATTAGTGCCTAAAGCCACGCCTTATGCTGAGCAAATTACCTATG
TAACTGATAGACCAGGTCATGATCGCCGTTATGCCATAGATGCAACAAAAATGAGTAATGAGCTTAAGTGGCAACCAGAA
GAAACCTTCGAAACAGGCCTTCGTAAAACCATTGAATGGTATTTAGCGAATCAAGAGTGGTGTCAGCATGTCCAAGATGG
TAGCTATCAGCGAGAGCGTTTAGGCATTTAA

Upstream 100 bases:

>100_bases
GACAGAACTCTGCTTTGCCCTTACGGTAAGCGTATTGTTCCGTTAACAGCTTTCTAACTTTAGTAAGCGTTACATTTTAT
ATTTTATCGAGGTATTTTTA

Downstream 100 bases:

>100_bases
TCTCTTTTATTATTTTGTTTTATGTCCTAGTTTTTCTTTAAACCTAGGATCTATTTATCTAGGAACTGATTTTACTATGA
AGGGAATTATTCTAGCCGGC

Product: dTDP-glucose 4,6 dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MKILVTGGAGFIGSAVVRFIINNTQDSVINVDKLTYAGNLESLLSVEKNQRYAFEQVDICDRVELDRVFNKYQPDAVMHL
AAESHVDRSITGPGDFIQTNIVGTYTLLEAARHYWMQLDAERKSAFRFHHISTDEVYGDLPHPDEVVPGSELALFTEITP
YAPSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKALPIYGKGDQIRDWLYVEDHA
RALYKVVTEGKIGETYNIGGHNEKQNLEVVQTICSILDSLVPKATPYAEQITYVTDRPGHDRRYAIDATKMSNELKWQPE
ETFETGLRKTIEWYLANQEWCQHVQDGSYQRERLGI

Sequences:

>Translated_356_residues
MKILVTGGAGFIGSAVVRFIINNTQDSVINVDKLTYAGNLESLLSVEKNQRYAFEQVDICDRVELDRVFNKYQPDAVMHL
AAESHVDRSITGPGDFIQTNIVGTYTLLEAARHYWMQLDAERKSAFRFHHISTDEVYGDLPHPDEVVPGSELALFTEITP
YAPSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKALPIYGKGDQIRDWLYVEDHA
RALYKVVTEGKIGETYNIGGHNEKQNLEVVQTICSILDSLVPKATPYAEQITYVTDRPGHDRRYAIDATKMSNELKWQPE
ETFETGLRKTIEWYLANQEWCQHVQDGSYQRERLGI
>Mature_356_residues
MKILVTGGAGFIGSAVVRFIINNTQDSVINVDKLTYAGNLESLLSVEKNQRYAFEQVDICDRVELDRVFNKYQPDAVMHL
AAESHVDRSITGPGDFIQTNIVGTYTLLEAARHYWMQLDAERKSAFRFHHISTDEVYGDLPHPDEVVPGSELALFTEITP
YAPSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKALPIYGKGDQIRDWLYVEDHA
RALYKVVTEGKIGETYNIGGHNEKQNLEVVQTICSILDSLVPKATPYAEQITYVTDRPGHDRRYAIDATKMSNELKWQPE
ETFETGLRKTIEWYLANQEWCQHVQDGSYQRERLGI

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=340, Percent_Identity=35.8823529411765, Blast_Score=208, Evalue=7e-54,
Organism=Homo sapiens, GI42516563, Length=347, Percent_Identity=25.0720461095101, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI56237023, Length=363, Percent_Identity=28.6501377410468, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI56118217, Length=363, Percent_Identity=28.6501377410468, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI189083684, Length=363, Percent_Identity=28.6501377410468, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1788353, Length=350, Percent_Identity=78.2857142857143, Blast_Score=585, Evalue=1e-168,
Organism=Escherichia coli, GI48994969, Length=359, Percent_Identity=71.866295264624, Blast_Score=547, Evalue=1e-157,
Organism=Escherichia coli, GI1786974, Length=358, Percent_Identity=24.5810055865922, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788366, Length=366, Percent_Identity=25.1366120218579, Blast_Score=78, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17568069, Length=338, Percent_Identity=38.1656804733728, Blast_Score=214, Evalue=6e-56,
Organism=Caenorhabditis elegans, GI115532424, Length=331, Percent_Identity=32.02416918429, Blast_Score=164, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI71982035, Length=375, Percent_Identity=27.4666666666667, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71982038, Length=377, Percent_Identity=27.3209549071618, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17539532, Length=339, Percent_Identity=25.3687315634218, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319493, Length=368, Percent_Identity=27.7173913043478, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21356223, Length=354, Percent_Identity=24.8587570621469, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19923002, Length=368, Percent_Identity=26.6304347826087, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40333; Mature: 40333

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGGAGFIGSAVVRFIINNTQDSVINVDKLTYAGNLESLLSVEKNQRYAFEQVDIC
CEEEEECCCCHHHHHHHHHHHCCCCCCEEEHHHEEECCCHHHHHHHHHCCCHHHHHHCHH
DRVELDRVFNKYQPDAVMHLAAESHVDRSITGPGDFIQTNIVGTYTLLEAARHYWMQLDA
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHCCH
ERKSAFRFHHISTDEVYGDLPHPDEVVPGSELALFTEITPYAPSSPYSASKASSDHLVRA
HHHHCEEEECCCHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHH
WLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKALPIYGKGDQIRDWLYVEDHA
HHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEHHHH
RALYKVVTEGKIGETYNIGGHNEKQNLEVVQTICSILDSLVPKATPYAEQITYVTDRPGH
HHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEECCCCC
DRRYAIDATKMSNELKWQPEETFETGLRKTIEWYLANQEWCQHVQDGSYQRERLGI
CCEEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHCCC
>Mature Secondary Structure
MKILVTGGAGFIGSAVVRFIINNTQDSVINVDKLTYAGNLESLLSVEKNQRYAFEQVDIC
CEEEEECCCCHHHHHHHHHHHCCCCCCEEEHHHEEECCCHHHHHHHHHCCCHHHHHHCHH
DRVELDRVFNKYQPDAVMHLAAESHVDRSITGPGDFIQTNIVGTYTLLEAARHYWMQLDA
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHCCH
ERKSAFRFHHISTDEVYGDLPHPDEVVPGSELALFTEITPYAPSSPYSASKASSDHLVRA
HHHHCEEEECCCHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHH
WLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKALPIYGKGDQIRDWLYVEDHA
HHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEHHHH
RALYKVVTEGKIGETYNIGGHNEKQNLEVVQTICSILDSLVPKATPYAEQITYVTDRPGH
HHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEECCCCC
DRRYAIDATKMSNELKWQPEETFETGLRKTIEWYLANQEWCQHVQDGSYQRERLGI
CCEEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7517391; 9097040; 9278503 [H]