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The map label for this gene is mdtE [H]

Identifier: 126174981

GI number: 126174981

Start: 3260566

End: 3261579

Strand: Direct

Name: mdtE [H]

Synonym: Sbal_2774

Alternate gene names: 126174981

Gene position: 3260566-3261579 (Clockwise)

Preceding gene: 126174980

Following gene: 126174982

Centisome position: 63.59

GC content: 49.9

Gene sequence:

>1014_bases
ATGACGTCAATTTCTCGCGTTTTTTTCTTACTGCTGGCGCTTGCCAGTACTCAAGTTCAGGCTGCCTCAGTCGAAACCAT
CGAAGTCTTAACTAAACCCTACGTCAATTGGGTCACCTTAGATGCCACCATAGAAGCAGTAAAAGCGGCGACTGTGTCAG
CGCAGACCTCTGGTCGTATCGTCAAACTCAACTACGATGTTAACGATGTTGTCCCCGAGGGAGCCGCCCTACTGGAGATC
ACCAGTAAAGAACAAGGCGCTGAACTGGCAAGCTTTGAGGCCGATTTAGCCAAAGCCAGAGCCTTGAATGTTGAAGCACA
GGCGCAATACAAACGTTATAAAGAGTTATTTCCCCAAGGCGCGATTTCTAAGGGCGCGATGGATGAAGCTACCGCCAATG
CTAAGGCCACAGAACGAGCCGTAACCGCAGCGCAAGCCCGCGTCATCAAAGCCAGCGAATCCTTAAAATACACTGTGGTA
TCAGCGCCTTTTAGTGGTGTAGTCACAGAGCGTTTTGTCGAACAGGGTGAAACGGTGAGCCCAGGACAAGCACTACTTTC
AGGGTTTTCCGCGAGTCAAATGCGCGCCATTACCCAAGTGCCGCAGCGTTATATCAATCAGCTGAAAAACGCCCCCGAAT
TCCTCGTCCGTTTAAGTGATGGCCGCGAGTTAACCTCAAAGGATCTCACTATTTTTAGTTTTGCCGATCCCGTCAGCCAC
AGCTATCAAGTACGCATTAATCTGCCTGAAAATGAAGCTAATCTTCAACCCGGCACGTGGGCAAAAGCTTCCTTTAAAAA
TGGCGAGCGAGAAAAAATCCAACTGCCCACCTCTGCGCTACTCACAATGAACGAACTGAGCAGCGTTTACCTTAAAAAGG
GCGAAGAATTTGTACTCACCCAAGTCCGTGTCGCTAGTCCCTTAGATGGCGAGGTCGAAGTGCTGGCGGGCCTGAAGTCG
GGCGACAAGGTTGCCGTTGATGCCTACCAAGTGTTGCTCAACAAAAAGCAATAA

Upstream 100 bases:

>100_bases
AAAATACACTATATTAAATTTTTCTAATAAATATTGATTGCCTATTGAAAACAACTCCATTAGAATTTGCTCATGAATTA
CATCTGGGATGATATGAATT

Downstream 100 bases:

>100_bases
ATTCCCTAGCGACTTAAGCTTTGTCGAGGTTGTTTTATGAAACATGCTCCAGTACCACAGACCACACTTGGGATTTCCGG
TCGAATTGCCGCCGCCTTCC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MTSISRVFFLLLALASTQVQAASVETIEVLTKPYVNWVTLDATIEAVKAATVSAQTSGRIVKLNYDVNDVVPEGAALLEI
TSKEQGAELASFEADLAKARALNVEAQAQYKRYKELFPQGAISKGAMDEATANAKATERAVTAAQARVIKASESLKYTVV
SAPFSGVVTERFVEQGETVSPGQALLSGFSASQMRAITQVPQRYINQLKNAPEFLVRLSDGRELTSKDLTIFSFADPVSH
SYQVRINLPENEANLQPGTWAKASFKNGEREKIQLPTSALLTMNELSSVYLKKGEEFVLTQVRVASPLDGEVEVLAGLKS
GDKVAVDAYQVLLNKKQ

Sequences:

>Translated_337_residues
MTSISRVFFLLLALASTQVQAASVETIEVLTKPYVNWVTLDATIEAVKAATVSAQTSGRIVKLNYDVNDVVPEGAALLEI
TSKEQGAELASFEADLAKARALNVEAQAQYKRYKELFPQGAISKGAMDEATANAKATERAVTAAQARVIKASESLKYTVV
SAPFSGVVTERFVEQGETVSPGQALLSGFSASQMRAITQVPQRYINQLKNAPEFLVRLSDGRELTSKDLTIFSFADPVSH
SYQVRINLPENEANLQPGTWAKASFKNGEREKIQLPTSALLTMNELSSVYLKKGEEFVLTQVRVASPLDGEVEVLAGLKS
GDKVAVDAYQVLLNKKQ
>Mature_336_residues
TSISRVFFLLLALASTQVQAASVETIEVLTKPYVNWVTLDATIEAVKAATVSAQTSGRIVKLNYDVNDVVPEGAALLEIT
SKEQGAELASFEADLAKARALNVEAQAQYKRYKELFPQGAISKGAMDEATANAKATERAVTAAQARVIKASESLKYTVVS
APFSGVVTERFVEQGETVSPGQALLSGFSASQMRAITQVPQRYINQLKNAPEFLVRLSDGRELTSKDLTIFSFADPVSHS
YQVRINLPENEANLQPGTWAKASFKNGEREKIQLPTSALLTMNELSSVYLKKGEEFVLTQVRVASPLDGEVEVLAGLKSG
DKVAVDAYQVLLNKKQ

Specific function: Part of a multidrug resistance efflux system that confers resistance to compounds such as rhodamine 6G, erythromycin, doxorubicin, ethidium bromide, TPP, SDS, deoxycholate, crystal violet and benzalkonium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=302, Percent_Identity=25.1655629139073, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786668, Length=338, Percent_Identity=28.4023668639053, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI87081951, Length=188, Percent_Identity=32.4468085106383, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87082036, Length=308, Percent_Identity=25, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 36536; Mature: 36405

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSISRVFFLLLALASTQVQAASVETIEVLTKPYVNWVTLDATIEAVKAATVSAQTSGRI
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHEEECCCCEE
VKLNYDVNDVVPEGAALLEITSKEQGAELASFEADLAKARALNVEAQAQYKRYKELFPQG
EEEECCHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
AISKGAMDEATANAKATERAVTAAQARVIKASESLKYTVVSAPFSGVVTERFVEQGETVS
CCCCCCCCHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHCCCCCC
PGQALLSGFSASQMRAITQVPQRYINQLKNAPEFLVRLSDGRELTSKDLTIFSFADPVSH
CHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHEEEECCCCEECCCCEEEEEECCCCCC
SYQVRINLPENEANLQPGTWAKASFKNGEREKIQLPTSALLTMNELSSVYLKKGEEFVLT
CEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCEEEEE
QVRVASPLDGEVEVLAGLKSGDKVAVDAYQVLLNKKQ
EEEECCCCCCCEEEEEECCCCCEEHHHHHHHHHCCCC
>Mature Secondary Structure 
TSISRVFFLLLALASTQVQAASVETIEVLTKPYVNWVTLDATIEAVKAATVSAQTSGRI
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHEEECCCCEE
VKLNYDVNDVVPEGAALLEITSKEQGAELASFEADLAKARALNVEAQAQYKRYKELFPQG
EEEECCHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
AISKGAMDEATANAKATERAVTAAQARVIKASESLKYTVVSAPFSGVVTERFVEQGETVS
CCCCCCCCHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHCCCCCC
PGQALLSGFSASQMRAITQVPQRYINQLKNAPEFLVRLSDGRELTSKDLTIFSFADPVSH
CHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHEEEECCCCEECCCCEEEEEECCCCCC
SYQVRINLPENEANLQPGTWAKASFKNGEREKIQLPTSALLTMNELSSVYLKKGEEFVLT
CEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCEEEEE
QVRVASPLDGEVEVLAGLKSGDKVAVDAYQVLLNKKQ
EEEECCCCCCCEEEEEECCCCCEEHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]