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The map label for this gene is trpCF [H]

Identifier: 126174912

GI number: 126174912

Start: 3170862

End: 3172349

Strand: Direct

Name: trpCF [H]

Synonym: Sbal_2704

Alternate gene names: 126174912

Gene position: 3170862-3172349 (Clockwise)

Preceding gene: 126174911

Following gene: 126174913

Centisome position: 61.84

GC content: 50.07

Gene sequence:

>1488_bases
ATGTCTCAAGTAGAAGTGACTCCAACAGCAATGACTCAAGACCTGCGTACTCAGCCAGAGGCAGCCACAAACGCTATGCC
AAAAAGTAATGTGTTAACCCGTATCGTCGATACCAAAACGGCCCATATTGCCGCCCTAAAACTGCGTTTCCCTGAGGCGA
GTCTGTCGCCAAAAATCTCTGACCGCAGTTTATTTTCCGCACTCAAGGCACCCAAGGCGGGTTACATTTTAGAATGCAAA
AAAGCCAGTCCGTCCAAAGGGCTTATCCGCGATGTGTTTGATGTAGAAGCCATCGCCGATATTTACGGTAAATATGCGGC
GGGCATTTCGGTATTAACCGACGAGCAATTTTTCCAAGGGGATATGGACTATATTCCTAAGGTGCGCGCTCGGGTGAACC
AACCGATTCTGTGCAAAGATTTTTTTGTGGATGAATACCAAATCAAACTCGCCGCTCACCAAGGCGCCGATGCGATTCTG
TTGATGCTGTCCGTGCTTGATGATGAACAATATCAAGGTCTAGCGAAAGAAGCCGCTAAGTATCAACTCGATGTACTGAC
CGAAGTCAGTAACGAAGAAGAACTTAAGCGCGCCATCGCATTAGATGCGCCGATTATTGGCATCAACAACCGTAACTTAA
GGGATTTAAGTACCGATCTCGCCACCACAGAAGCCTTAGCGCCACACATTGGCAGCGACCGCGTGGTGATCAGCGAATCT
GGCATCTACAACAATGAGCAAGTACGTCGCCTAAGTCCGCTGGTTGATGGCTTCCTTGTCGGCAGCTCGATTATGGCTGA
GGCTGATATCGACTTGGCCTGCCGTAAATTGATCTTCGGTCACAATAAAGTCTGCGGCCTCACCCGTGTGGAAGATATTC
GCGCCGCGGCAACAGCAGGCGCTGTCTATGGCGGACTGATTTTTGCTGAAAAATCCCCACGCGCGTTAACCCTTAATGCC
GCAGAACAACTACTCAAGGAGTACCGAGCATCTGATGCCCCCGCCATTGAATTTGTCGGCGTGTTTGTTAATGCCGCGCC
AAGCACCATCACCGATATCGCTGCCAGTTTGCAGTTATCTGCCGTACAGCTGCATGGCACTGAGACTGAGCTTGAAATAA
CACAGCTCGCTGAGCGACTTGAACAAGCGGGACTCACCACCCAAATTTGGAAAGCCGTCAGCGTCGATGCGCAAACGGGT
GAGCTTGGCAACTTACCTCAGGGAGCACAGCGTTATCTGTTTGATAGTAAAACGGCAGGTCAATTTGGCGGCTCAGGCCA
AGCCTTTAATTGGCAAAATATTGATGTGAAGTCCTTAGGCCAGCAAAAGGCCCATGCCATGTTGGCGGGCGGACTCAACG
CTGACAATGCAGCCAGTGCCAATGCCCAAGGATTTTACGGTTTAGATTTTAATTCTGGCCTAGAAACCGCCGCAGGAATT
AAGTCGGCGCAACTGATCCAAACGGCTTTTACCCATTTACGTCTCTAG

Upstream 100 bases:

>100_bases
GCTACCATTCAAAGCGGCAAAGCCTTTGAATTGTTAAGCCAGCTCGCAAAAGTCAGCGGTGAAGCCCTAGTCAACGGTCA
AGAAAGAGGAAGATAAGCGA

Downstream 100 bases:

>100_bases
CCTATCGCGCTAAGCAAGAAGCCCTAGATGCAGTTGGTGCTCGACAAGCGTGATGCTCGACAAAAATATGAACCTATTAA
TGCCAGTGGCCAAAATAGAT

Product: bifunctional indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase

Products: NA

Alternate protein names: Indole-3-glycerol phosphate synthase; IGPS; N-(5'-phospho-ribosyl)anthranilate isomerase; PRAI [H]

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MSQVEVTPTAMTQDLRTQPEAATNAMPKSNVLTRIVDTKTAHIAALKLRFPEASLSPKISDRSLFSALKAPKAGYILECK
KASPSKGLIRDVFDVEAIADIYGKYAAGISVLTDEQFFQGDMDYIPKVRARVNQPILCKDFFVDEYQIKLAAHQGADAIL
LMLSVLDDEQYQGLAKEAAKYQLDVLTEVSNEEELKRAIALDAPIIGINNRNLRDLSTDLATTEALAPHIGSDRVVISES
GIYNNEQVRRLSPLVDGFLVGSSIMAEADIDLACRKLIFGHNKVCGLTRVEDIRAAATAGAVYGGLIFAEKSPRALTLNA
AEQLLKEYRASDAPAIEFVGVFVNAAPSTITDIAASLQLSAVQLHGTETELEITQLAERLEQAGLTTQIWKAVSVDAQTG
ELGNLPQGAQRYLFDSKTAGQFGGSGQAFNWQNIDVKSLGQQKAHAMLAGGLNADNAASANAQGFYGLDFNSGLETAAGI
KSAQLIQTAFTHLRL

Sequences:

>Translated_495_residues
MSQVEVTPTAMTQDLRTQPEAATNAMPKSNVLTRIVDTKTAHIAALKLRFPEASLSPKISDRSLFSALKAPKAGYILECK
KASPSKGLIRDVFDVEAIADIYGKYAAGISVLTDEQFFQGDMDYIPKVRARVNQPILCKDFFVDEYQIKLAAHQGADAIL
LMLSVLDDEQYQGLAKEAAKYQLDVLTEVSNEEELKRAIALDAPIIGINNRNLRDLSTDLATTEALAPHIGSDRVVISES
GIYNNEQVRRLSPLVDGFLVGSSIMAEADIDLACRKLIFGHNKVCGLTRVEDIRAAATAGAVYGGLIFAEKSPRALTLNA
AEQLLKEYRASDAPAIEFVGVFVNAAPSTITDIAASLQLSAVQLHGTETELEITQLAERLEQAGLTTQIWKAVSVDAQTG
ELGNLPQGAQRYLFDSKTAGQFGGSGQAFNWQNIDVKSLGQQKAHAMLAGGLNADNAASANAQGFYGLDFNSGLETAAGI
KSAQLIQTAFTHLRL
>Mature_494_residues
SQVEVTPTAMTQDLRTQPEAATNAMPKSNVLTRIVDTKTAHIAALKLRFPEASLSPKISDRSLFSALKAPKAGYILECKK
ASPSKGLIRDVFDVEAIADIYGKYAAGISVLTDEQFFQGDMDYIPKVRARVNQPILCKDFFVDEYQIKLAAHQGADAILL
MLSVLDDEQYQGLAKEAAKYQLDVLTEVSNEEELKRAIALDAPIIGINNRNLRDLSTDLATTEALAPHIGSDRVVISESG
IYNNEQVRRLSPLVDGFLVGSSIMAEADIDLACRKLIFGHNKVCGLTRVEDIRAAATAGAVYGGLIFAEKSPRALTLNAA
EQLLKEYRASDAPAIEFVGVFVNAAPSTITDIAASLQLSAVQLHGTETELEITQLAERLEQAGLTTQIWKAVSVDAQTGE
LGNLPQGAQRYLFDSKTAGQFGGSGQAFNWQNIDVKSLGQQKAHAMLAGGLNADNAASANAQGFYGLDFNSGLETAAGIK
SAQLIQTAFTHLRL

Specific function: Bifunctional enzyme that catalyzes two sequential steps of tryptophan biosynthetic pathway. The first reaction is catalyzed by the isomerase, coded by the trpF domain; the second reaction is catalyzed by the synthase, coded by the trpC domain [H]

COG id: COG0134

COG function: function code E; Indole-3-glycerol phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the trpF family [H]

Homologues:

Organism=Escherichia coli, GI87081863, Length=470, Percent_Identity=49.3617021276596, Blast_Score=405, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6322638, Length=238, Percent_Identity=34.453781512605, Blast_Score=103, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013798
- InterPro:   IPR001468
- InterPro:   IPR001240
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00218 IGPS; PF00697 PRAI [H]

EC number: =4.1.1.48; =5.3.1.24 [H]

Molecular weight: Translated: 53290; Mature: 53159

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00614 IGPS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVEVTPTAMTQDLRTQPEAATNAMPKSNVLTRIVDTKTAHIAALKLRFPEASLSPKIS
CCCEECCHHHHHHHHHCCCCHHHCCCCCHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCC
DRSLFSALKAPKAGYILECKKASPSKGLIRDVFDVEAIADIYGKYAAGISVLTDEQFFQG
HHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHCC
DMDYIPKVRARVNQPILCKDFFVDEYQIKLAAHQGADAILLMLSVLDDEQYQGLAKEAAK
CHHHHHHHHHHCCCCEEEHHHCCCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHH
YQLDVLTEVSNEEELKRAIALDAPIIGINNRNLRDLSTDLATTEALAPHIGSDRVVISES
HHHHHHHHCCCHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECC
GIYNNEQVRRLSPLVDGFLVGSSIMAEADIDLACRKLIFGHNKVCGLTRVEDIRAAATAG
CCCCCHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
AVYGGLIFAEKSPRALTLNAAEQLLKEYRASDAPAIEFVGVFVNAAPSTITDIAASLQLS
HHHHCEEEECCCCCEEEHHHHHHHHHHHCCCCCCCEEEEEEHHCCCCHHHHHHHHHHEEE
AVQLHGTETELEITQLAERLEQAGLTTQIWKAVSVDAQTGELGNLPQGAQRYLFDSKTAG
EEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCC
QFGGSGQAFNWQNIDVKSLGQQKAHAMLAGGLNADNAASANAQGFYGLDFNSGLETAAGI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCHHHCC
KSAQLIQTAFTHLRL
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQVEVTPTAMTQDLRTQPEAATNAMPKSNVLTRIVDTKTAHIAALKLRFPEASLSPKIS
CCEECCHHHHHHHHHCCCCHHHCCCCCHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCC
DRSLFSALKAPKAGYILECKKASPSKGLIRDVFDVEAIADIYGKYAAGISVLTDEQFFQG
HHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHCC
DMDYIPKVRARVNQPILCKDFFVDEYQIKLAAHQGADAILLMLSVLDDEQYQGLAKEAAK
CHHHHHHHHHHCCCCEEEHHHCCCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHH
YQLDVLTEVSNEEELKRAIALDAPIIGINNRNLRDLSTDLATTEALAPHIGSDRVVISES
HHHHHHHHCCCHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECC
GIYNNEQVRRLSPLVDGFLVGSSIMAEADIDLACRKLIFGHNKVCGLTRVEDIRAAATAG
CCCCCHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
AVYGGLIFAEKSPRALTLNAAEQLLKEYRASDAPAIEFVGVFVNAAPSTITDIAASLQLS
HHHHCEEEECCCCCEEEHHHHHHHHHHHCCCCCCCEEEEEEHHCCCCHHHHHHHHHHEEE
AVQLHGTETELEITQLAERLEQAGLTTQIWKAVSVDAQTGELGNLPQGAQRYLFDSKTAG
EEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCC
QFGGSGQAFNWQNIDVKSLGQQKAHAMLAGGLNADNAASANAQGFYGLDFNSGLETAAGI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCHHHCC
KSAQLIQTAFTHLRL
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]