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The map label for this gene is trpG [H]

Identifier: 126174910

GI number: 126174910

Start: 3169164

End: 3169787

Strand: Direct

Name: trpG [H]

Synonym: Sbal_2702

Alternate gene names: 126174910

Gene position: 3169164-3169787 (Clockwise)

Preceding gene: 126174909

Following gene: 126174911

Centisome position: 61.81

GC content: 52.08

Gene sequence:

>624_bases
ATGAAACTCTATCTACTCGATAACTTTGACTCCTTTACCTACAACTTAGTGGATCAATTTCGCAGCCTAGGCTGTGAAGT
GGTGATTTACCGCAACGATGTTGCCGCCGACTTTATCGCTGAAAAACTGCTTAACGAACAAGAACCCGCAGCCCTAGTGT
TATCGCCAGGTCCTGGTGCGCCCCATGAAGCAGGCTCTATGATGGCGCTTATCGCCAAAGTCGCCGGTAAAGTGCCTATG
CTGGGTATCTGCCTTGGGCATCAAGCTATGGTGGAATATTACGGCGGCACAGTGGCGCGCGCGCCTTTTGTGGTGCATGG
CAAGGCAAGCCCGACTTTCCATGATGGCACTGGCGTATTTGCCGGTTTGCCCTCTCCCCTGCCCGTTGCCCGTTATCACA
GTCTAGTCGCCACTCACGTGCCTGATTGCTTGCAAGTGATTGCAACGACTGACGCTATGCCGATGGCAATTCTGCACCCT
GAGCACAGAGCCGTTGGCTTTCAGTTCCACCCTGAATCGATTCTCACCACCTTAGGTAGCACACTGCTCATTCAAACCTT
GGCCTTTCTTACCCAAGAGATGACATTTCTACCCCAAGACATGACAACAGGAGTAACCGCATGA

Upstream 100 bases:

>100_bases
GGGTGCAGGACTGCGAGTCAATGAATCGCCAGCGAATGACGCGTCAGCTCAATCGACTTTTGTACAATCCACTTCAGTAC
CATCTAAATAGGAGCCAAAC

Downstream 100 bases:

>100_bases
GTACCAACCCTATTCAGCCTTTACTCGACGTTTTATACCAAGGTAAAAGTTTAAGCCGCGAGCAAACCGCCGAACTTTTT
GGCGCGTTAATTCGCGGAGA

Product: anthranilate synthase component II

Products: NA

Alternate protein names: Glutamine amido-transferase [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MKLYLLDNFDSFTYNLVDQFRSLGCEVVIYRNDVAADFIAEKLLNEQEPAALVLSPGPGAPHEAGSMMALIAKVAGKVPM
LGICLGHQAMVEYYGGTVARAPFVVHGKASPTFHDGTGVFAGLPSPLPVARYHSLVATHVPDCLQVIATTDAMPMAILHP
EHRAVGFQFHPESILTTLGSTLLIQTLAFLTQEMTFLPQDMTTGVTA

Sequences:

>Translated_207_residues
MKLYLLDNFDSFTYNLVDQFRSLGCEVVIYRNDVAADFIAEKLLNEQEPAALVLSPGPGAPHEAGSMMALIAKVAGKVPM
LGICLGHQAMVEYYGGTVARAPFVVHGKASPTFHDGTGVFAGLPSPLPVARYHSLVATHVPDCLQVIATTDAMPMAILHP
EHRAVGFQFHPESILTTLGSTLLIQTLAFLTQEMTFLPQDMTTGVTA
>Mature_207_residues
MKLYLLDNFDSFTYNLVDQFRSLGCEVVIYRNDVAADFIAEKLLNEQEPAALVLSPGPGAPHEAGSMMALIAKVAGKVPM
LGICLGHQAMVEYYGGTVARAPFVVHGKASPTFHDGTGVFAGLPSPLPVARYHSLVATHVPDCLQVIATTDAMPMAILHP
EHRAVGFQFHPESILTTLGSTLLIQTLAFLTQEMTFLPQDMTTGVTA

Specific function: Tryptophan biosynthesis; first step. Tryptophan biosynthesis; second step. [C]

COG id: COG0512

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787517, Length=192, Percent_Identity=56.25, Blast_Score=216, Evalue=1e-57,
Organism=Escherichia coli, GI1789760, Length=190, Percent_Identity=47.3684210526316, Blast_Score=164, Evalue=3e-42,
Organism=Escherichia coli, GI1788854, Length=200, Percent_Identity=26, Blast_Score=63, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322638, Length=187, Percent_Identity=41.1764705882353, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: 945 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR006221 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 22253; Mature: 22253

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYLLDNFDSFTYNLVDQFRSLGCEVVIYRNDVAADFIAEKLLNEQEPAALVLSPGPGA
CEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCCCCCEEEEECCCCC
PHEAGSMMALIAKVAGKVPMLGICLGHQAMVEYYGGTVARAPFVVHGKASPTFHDGTGVF
CCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHCCCEECCCEEEECCCCCCEECCCCEE
AGLPSPLPVARYHSLVATHVPDCLQVIATTDAMPMAILHPEHRAVGFQFHPESILTTLGS
ECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEECEEECHHHHHHHHHH
TLLIQTLAFLTQEMTFLPQDMTTGVTA
HHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKLYLLDNFDSFTYNLVDQFRSLGCEVVIYRNDVAADFIAEKLLNEQEPAALVLSPGPGA
CEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCCCCCEEEEECCCCC
PHEAGSMMALIAKVAGKVPMLGICLGHQAMVEYYGGTVARAPFVVHGKASPTFHDGTGVF
CCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHCCCEECCCEEEECCCCCCEECCCCEE
AGLPSPLPVARYHSLVATHVPDCLQVIATTDAMPMAILHPEHRAVGFQFHPESILTTLGS
ECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEECEEECHHHHHHHHHH
TLLIQTLAFLTQEMTFLPQDMTTGVTA
HHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]