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The map label for this gene is dusC [H]

Identifier: 126174794

GI number: 126174794

Start: 3026143

End: 3027096

Strand: Direct

Name: dusC [H]

Synonym: Sbal_2581

Alternate gene names: 126174794

Gene position: 3026143-3027096 (Clockwise)

Preceding gene: 126174778

Following gene: 126174795

Centisome position: 59.02

GC content: 49.9

Gene sequence:

>954_bases
GTGCGTGTGATTTTGGCTCCAATGGAAGGCGTAGTAGACGCTTTGATGCGTGACATCCTATCGACCATCAATCCCTACGA
TTTGCTCGTCACCGAATTTGTGCGAGTGGTAGACCAACTGCTGCCTGAAAAGGTCTTTCTCAAACTGTGTCCTGAACTGC
TCAACAACGGTAATACTCCTTCAGGTACGCCAGTACGAGTACAATTGCTTGGTCAAGAACCCCAATGTATGGGCGAAAAT
GCCCTGCGTGCCATTGAGCTAGGCTCACACGGTGTTGATGCAAACTTTGGCTGTCCAGCAAAAATTGTTAACCGCAGTAA
AGGTGGCGCCGTATTATTGCAATATCCTGAGAGTGTGCACGATATTGTCAAAGCCATGCGTCAAGCAGTACCAAAAGAGC
ATGCCGTAACGGCCAAAATACGTTTGGGCTACGAAGATAAGTCACTGTTCATGGAAAATGCTATCGCAGTGTATGAAGCC
GGAGCAACTGAGCTTGCCATCCATGCGCGCAGTAAAGTCGACGGCTACAGACCGCCCGCCTACTGGGAATACATCACAGA
AGTACGCAAACGTTTGCCGATCCCGGTGATCGCCAACGGCGAGATTTGGAACAGCGATGATGCTAAGCGTTGCATCGAAG
TCACCGGCTGCGACAGTATTATGATCGGCCGCGGGGCGATTTCATTGCCGAACTTGGCCGATACAATCAAGACAGGTGCA
ACCCCATATACTTGGGCGCAAACCTTAGGGCTGATGCTCAGTTATACCCAAAGGGAACTCAATGGCCGCAAGAGCGACTA
TTATCCCGCCCGAATCAAACAGTGGTTTACCTATCTCAATCGTCAATATCCCGAAGCCGATACCTTGTTCAGGGAGCTGC
GCGTGTTTAAAACCACAGAAGAAATCGTCGCGGTATTAGAAGCTGCGCAGCGAAACTTAGGCAACGAATCATAG

Upstream 100 bases:

>100_bases
ATGAATGCGCCTATTAATCCGCATTCACTCAGCCACTTTGTAACTTAAGGCCGATAAGGTAGAATGCTCGCCCTTTGCAA
ATAGATATGGGTTTACTGCA

Downstream 100 bases:

>100_bases
AAGAGTGAGCTACCACAGAACGGCAACAAGCCATATAAGCGTTAGAAATCGAGATTCAATGCGCGGCAATCTGAGCCATG
AAAGGGACTTCGCCATAGCA

Product: dihydrouridine synthase DuS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MRVILAPMEGVVDALMRDILSTINPYDLLVTEFVRVVDQLLPEKVFLKLCPELLNNGNTPSGTPVRVQLLGQEPQCMGEN
ALRAIELGSHGVDANFGCPAKIVNRSKGGAVLLQYPESVHDIVKAMRQAVPKEHAVTAKIRLGYEDKSLFMENAIAVYEA
GATELAIHARSKVDGYRPPAYWEYITEVRKRLPIPVIANGEIWNSDDAKRCIEVTGCDSIMIGRGAISLPNLADTIKTGA
TPYTWAQTLGLMLSYTQRELNGRKSDYYPARIKQWFTYLNRQYPEADTLFRELRVFKTTEEIVAVLEAAQRNLGNES

Sequences:

>Translated_317_residues
MRVILAPMEGVVDALMRDILSTINPYDLLVTEFVRVVDQLLPEKVFLKLCPELLNNGNTPSGTPVRVQLLGQEPQCMGEN
ALRAIELGSHGVDANFGCPAKIVNRSKGGAVLLQYPESVHDIVKAMRQAVPKEHAVTAKIRLGYEDKSLFMENAIAVYEA
GATELAIHARSKVDGYRPPAYWEYITEVRKRLPIPVIANGEIWNSDDAKRCIEVTGCDSIMIGRGAISLPNLADTIKTGA
TPYTWAQTLGLMLSYTQRELNGRKSDYYPARIKQWFTYLNRQYPEADTLFRELRVFKTTEEIVAVLEAAQRNLGNES
>Mature_317_residues
MRVILAPMEGVVDALMRDILSTINPYDLLVTEFVRVVDQLLPEKVFLKLCPELLNNGNTPSGTPVRVQLLGQEPQCMGEN
ALRAIELGSHGVDANFGCPAKIVNRSKGGAVLLQYPESVHDIVKAMRQAVPKEHAVTAKIRLGYEDKSLFMENAIAVYEA
GATELAIHARSKVDGYRPPAYWEYITEVRKRLPIPVIANGEIWNSDDAKRCIEVTGCDSIMIGRGAISLPNLADTIKTGA
TPYTWAQTLGLMLSYTQRELNGRKSDYYPARIKQWFTYLNRQYPEADTLFRELRVFKTTEEIVAVLEAAQRNLGNES

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=229, Percent_Identity=29.6943231441048, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI239788462, Length=229, Percent_Identity=29.6943231441048, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI31742496, Length=170, Percent_Identity=28.2352941176471, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI40807366, Length=259, Percent_Identity=30.1158301158301, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI8923374, Length=263, Percent_Identity=26.2357414448669, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1788462, Length=309, Percent_Identity=52.4271844660194, Blast_Score=334, Evalue=5e-93,
Organism=Escherichia coli, GI1789660, Length=325, Percent_Identity=31.0769230769231, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI145693211, Length=281, Percent_Identity=27.4021352313167, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25144369, Length=172, Percent_Identity=33.1395348837209, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17507177, Length=169, Percent_Identity=31.3609467455621, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=200, Percent_Identity=28, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24580595, Length=193, Percent_Identity=33.160621761658, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19920448, Length=193, Percent_Identity=33.160621761658, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24585320, Length=235, Percent_Identity=29.3617021276596, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19921524, Length=152, Percent_Identity=32.2368421052632, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35384; Mature: 35384

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVILAPMEGVVDALMRDILSTINPYDLLVTEFVRVVDQLLPEKVFLKLCPELLNNGNTP
CEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SGTPVRVQLLGQEPQCMGENALRAIELGSHGVDANFGCPAKIVNRSKGGAVLLQYPESVH
CCCCEEEEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCEEEEECCHHHH
DIVKAMRQAVPKEHAVTAKIRLGYEDKSLFMENAIAVYEAGATELAIHARSKVDGYRPPA
HHHHHHHHHCCHHHCEEEEEEECCCHHHHHHHHHHHHEECCCHHEEEEEHHHCCCCCCCH
YWEYITEVRKRLPIPVIANGEIWNSDDAKRCIEVTGCDSIMIGRGAISLPNLADTIKTGA
HHHHHHHHHHHCCCCEEECCCEECCCHHHHHEEECCCCEEEECCCCCCCCCHHHHHHCCC
TPYTWAQTLGLMLSYTQRELNGRKSDYYPARIKQWFTYLNRQYPEADTLFRELRVFKTTE
CCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EIVAVLEAAQRNLGNES
HHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRVILAPMEGVVDALMRDILSTINPYDLLVTEFVRVVDQLLPEKVFLKLCPELLNNGNTP
CEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SGTPVRVQLLGQEPQCMGENALRAIELGSHGVDANFGCPAKIVNRSKGGAVLLQYPESVH
CCCCEEEEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCEEEEECCHHHH
DIVKAMRQAVPKEHAVTAKIRLGYEDKSLFMENAIAVYEAGATELAIHARSKVDGYRPPA
HHHHHHHHHCCHHHCEEEEEEECCCHHHHHHHHHHHHEECCCHHEEEEEHHHCCCCCCCH
YWEYITEVRKRLPIPVIANGEIWNSDDAKRCIEVTGCDSIMIGRGAISLPNLADTIKTGA
HHHHHHHHHHHCCCCEEECCCEECCCHHHHHEEECCCCEEEECCCCCCCCCHHHHHHCCC
TPYTWAQTLGLMLSYTQRELNGRKSDYYPARIKQWFTYLNRQYPEADTLFRELRVFKTTE
CCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EIVAVLEAAQRNLGNES
HHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]