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The map label for this gene is ldh [H]

Identifier: 126174696

GI number: 126174696

Start: 2897004

End: 2898059

Strand: Direct

Name: ldh [H]

Synonym: Sbal_2483

Alternate gene names: 126174696

Gene position: 2897004-2898059 (Clockwise)

Preceding gene: 126174694

Following gene: 126174697

Centisome position: 56.5

GC content: 48.48

Gene sequence:

>1056_bases
GTGTATAGCGGAGAAATTCACGTGGCTGTATTTAATCATGTATCCTTTGACGAACATGAACAGGTCGTATTCTGTCATGA
TAAAGAAAGTGGCTTAAAGGCCATTGTCGCCATCCATAACACCAACCTTGGCCCTGCTGTGGGTGGTTGCCGGATGTGGA
ATTACCAATCGGATGATGAAGCCCTGACTGACGTATTACGCCTCTCTCGTGGCATGACTTACAAAAACGCCCTTGCCGGT
TTAACCATGGGCGGCGGCAAATCAGTGATCATCGCCGATCCAAAAAGACAAGACCGCGAAGCCCTATTCCGCGCTTTTGG
CCGCTTCATCAATAGTCTCGGCGGACGCTATTATTCCGCTGAAGACGTGGGTACTACCACGGCAGATATCATGATTGCCC
ATGAAGAAACCCCTTATATGGCAGGTCTTGAAGGCAAGAGTGGCGATCCATCACCGTTCACTGCCCTTGGCACTTATTTA
GGCATCAAAGCTGCGGTTAAACATAGACTCGGTTTAGACAGCCTAAAAGGCCTTAAAATCGCCGTTCAAGGCGTAGGTCA
CGTAGGCTATTACTTGTGCAAACACTTACATAACGAAGGCGCTGAACTGATTGTTACCGACATTCATCAAGCATCATTAG
ACAGAGTCGCTACCGACTTTGGTGCGATTGTTGTCGCGCCTCAAGAAATCTACGCGCAAGATGTGGATGTGTATGCTCCT
TGCGCCCTCGGCGCAACTTTAAACGATGCGACCTTACCGCAACTGAAAGCCAAAATTGTTGCTGGCTGTGCCAACAACCA
ATTAGCTGAAGTACGCCACGGTGAACAGCTGAAAGAAATGGGCATACTGTACGCGCCAGACTATGTGATCAACGCAGGCG
GCATTATCAACGTGTCGTTCGAAAAAGATTACGATGCGGCGAAATCGACCGCTAAAGTAGAAGAAATTTACAACACCCTG
CTGAAAATCTTCAGCCAAGCTGATGCACAGAACCGTACAACCGGCGCTGTGGCTGATGAAATGGCGAAAGCCATTATCGA
AGCCGCTAAAAAGTAA

Upstream 100 bases:

>100_bases
TTCGGTCATTATGCATGGTTGAGAGTATCGCTAAATGCTATGGTTCTGCAATAAATATAACAAGCAGTCGATGGGGACTC
ACCTCGTCGGCTTTTTCATT

Downstream 100 bases:

>100_bases
TTTTTCTGCCCGCGTAACATCCCCAAAAGCGACCTCTGGTCGCTTTTTTGTTATCTAAAATTTAATCTAGCGCTAAGCTC
ATGGTTTAGCCGCGCTTTTA

Product: Glu/Leu/Phe/Val dehydrogenase dimerisation region

Products: NA

Alternate protein names: LeuDH [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MYSGEIHVAVFNHVSFDEHEQVVFCHDKESGLKAIVAIHNTNLGPAVGGCRMWNYQSDDEALTDVLRLSRGMTYKNALAG
LTMGGGKSVIIADPKRQDREALFRAFGRFINSLGGRYYSAEDVGTTTADIMIAHEETPYMAGLEGKSGDPSPFTALGTYL
GIKAAVKHRLGLDSLKGLKIAVQGVGHVGYYLCKHLHNEGAELIVTDIHQASLDRVATDFGAIVVAPQEIYAQDVDVYAP
CALGATLNDATLPQLKAKIVAGCANNQLAEVRHGEQLKEMGILYAPDYVINAGGIINVSFEKDYDAAKSTAKVEEIYNTL
LKIFSQADAQNRTTGAVADEMAKAIIEAAKK

Sequences:

>Translated_351_residues
MYSGEIHVAVFNHVSFDEHEQVVFCHDKESGLKAIVAIHNTNLGPAVGGCRMWNYQSDDEALTDVLRLSRGMTYKNALAG
LTMGGGKSVIIADPKRQDREALFRAFGRFINSLGGRYYSAEDVGTTTADIMIAHEETPYMAGLEGKSGDPSPFTALGTYL
GIKAAVKHRLGLDSLKGLKIAVQGVGHVGYYLCKHLHNEGAELIVTDIHQASLDRVATDFGAIVVAPQEIYAQDVDVYAP
CALGATLNDATLPQLKAKIVAGCANNQLAEVRHGEQLKEMGILYAPDYVINAGGIINVSFEKDYDAAKSTAKVEEIYNTL
LKIFSQADAQNRTTGAVADEMAKAIIEAAKK
>Mature_351_residues
MYSGEIHVAVFNHVSFDEHEQVVFCHDKESGLKAIVAIHNTNLGPAVGGCRMWNYQSDDEALTDVLRLSRGMTYKNALAG
LTMGGGKSVIIADPKRQDREALFRAFGRFINSLGGRYYSAEDVGTTTADIMIAHEETPYMAGLEGKSGDPSPFTALGTYL
GIKAAVKHRLGLDSLKGLKIAVQGVGHVGYYLCKHLHNEGAELIVTDIHQASLDRVATDFGAIVVAPQEIYAQDVDVYAP
CALGATLNDATLPQLKAKIVAGCANNQLAEVRHGEQLKEMGILYAPDYVINAGGIINVSFEKDYDAAKSTAKVEEIYNTL
LKIFSQADAQNRTTGAVADEMAKAIIEAAKK

Specific function: Functions catabolically in the bacterial metabolism of branched-chain L-amino acids, and plays an important role in spore germination in cooperation with alanine dehydrogenase [H]

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=314, Percent_Identity=24.8407643312102, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI4885281, Length=314, Percent_Identity=24.8407643312102, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788059, Length=323, Percent_Identity=25.3869969040248, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR016211
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.9 [H]

Molecular weight: Translated: 37822; Mature: 37822

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSGEIHVAVFNHVSFDEHEQVVFCHDKESGLKAIVAIHNTNLGPAVGGCRMWNYQSDDE
CCCCEEEEEEEECCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCEEECCCCCCHH
ALTDVLRLSRGMTYKNALAGLTMGGGKSVIIADPKRQDREALFRAFGRFINSLGGRYYSA
HHHHHHHHHCCCCHHHHHHCEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEC
EDVGTTTADIMIAHEETPYMAGLEGKSGDPSPFTALGTYLGIKAAVKHRLGLDSLKGLKI
CCCCCCEEEEEEEECCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEE
AVQGVGHVGYYLCKHLHNEGAELIVTDIHQASLDRVATDFGAIVVAPQEIYAQDVDVYAP
EEECCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCEEEEECHHHHHCCCCEEEC
CALGATLNDATLPQLKAKIVAGCANNQLAEVRHGEQLKEMGILYAPDYVINAGGIINVSF
EECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEECCCEEEECCCEEEEEE
EKDYDAAKSTAKVEEIYNTLLKIFSQADAQNRTTGAVADEMAKAIIEAAKK
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYSGEIHVAVFNHVSFDEHEQVVFCHDKESGLKAIVAIHNTNLGPAVGGCRMWNYQSDDE
CCCCEEEEEEEECCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCEEECCCCCCHH
ALTDVLRLSRGMTYKNALAGLTMGGGKSVIIADPKRQDREALFRAFGRFINSLGGRYYSA
HHHHHHHHHCCCCHHHHHHCEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEC
EDVGTTTADIMIAHEETPYMAGLEGKSGDPSPFTALGTYLGIKAAVKHRLGLDSLKGLKI
CCCCCCEEEEEEEECCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEE
AVQGVGHVGYYLCKHLHNEGAELIVTDIHQASLDRVATDFGAIVVAPQEIYAQDVDVYAP
EEECCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCEEEEECHHHHHCCCCEEEC
CALGATLNDATLPQLKAKIVAGCANNQLAEVRHGEQLKEMGILYAPDYVINAGGIINVSF
EECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEECCCEEEECCCEEEEEE
EKDYDAAKSTAKVEEIYNTLLKIFSQADAQNRTTGAVADEMAKAIIEAAKK
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3069133; 1400267 [H]