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The map label for this gene is poxB [H]

Identifier: 126174509

GI number: 126174509

Start: 2668932

End: 2670668

Strand: Reverse

Name: poxB [H]

Synonym: Sbal_2294

Alternate gene names: 126174509

Gene position: 2670668-2668932 (Counterclockwise)

Preceding gene: 126174510

Following gene: 126174503

Centisome position: 52.09

GC content: 43.75

Gene sequence:

>1737_bases
ATGTCAAAAAACGTAGCGGATCATCTAGTCGAAATGCTAGTAGAAGCGGGTGTTAAGCGAGTTTATGCCGTGACTGGCGA
TAGTCTTAATCCGGTCAATGATGCTGTACGAAGGGATGGCAGATTGCAATGGATCCATGTCAGACACGAGGAAGCTGGCG
CTTATGCGGCTTCTATGGAAGCTGAGCTAAATGGTATAGGTTGCTGCATGGGTAGCAGTGGTCCTGGGCATGTTCATTTA
GTCAATGGACTCTATGATGCCAATAGAGCTGAAAATCCCGTTATTGCGATTGCTTCAACTATCCATACTGAAAAAATGGG
ATTAGATAATTTTCAAGAGACAAGACCCGAGTCGTTATTTCAAGATTGTTCAAAATATGTGTTTCTCGCCAATACGCCTA
AACAAGCTGTCAATGGTTTACAAACGGCGATTCAGCATGCCATCAGCAAGAAAGGGGTCGCTGTGCTCGGGCTTCCCGGT
GATGTGGCTTCAGCAAGTCTAGAATCTGTGCATACGGCTAATAAAAATTTCTATACCCAACCCAGAATAACCCCAAGCCA
TAAACAGCTTCAAGAATTGGCAAGCGTCATTAATCAACATGAAAAAGTGATGCTGTTTTGCGGCCATGGTTGTCGCTATG
CCGTTGAAGAAGTGATGGCATTGGCCAAAAGGCTTAATGCCCCTTTGGGATATAGCTTTCGAGGCAAAATATTTTTTGAA
CGTAAAGAAAATCCTTATGCCGTTGGATTAAATGGGCTGTTAGGCAATAAATCCGGTTTTGAAGCCATGCATCAAGCCGA
TGTCCTGTTGATGCTGGGCACGGATTTTCCTTATAGTGAGTTCCTTCCCGAAAAATGCAAAATTATTCAAGTCGATACTA
AACCCGAAAAATTAGGTCGTAGAGCTAAAGTGGATTTTGGCTATTGTGGTGATATTAAATCAACCCTTGAAGATCTCATG
CCGTTAATCAATGCCAAAACATCCACGGATTTTCTCGACAAAACCCGTTCACTGCACCAAGAAATTGAAGCTATCTATGA
GAGCTATGTGGAAGAAAAGGGCTCCGAAAAAAATATTCACCCAGAATATGTTGCCTATTTGGTGGATAAACTCGCCGATG
ACGATGCGATATTTACCGTTGATACGGGGATGAGCGCCGTGTGGGCCGCGCGTTATTTAAAGGCGGGAGCCAATCGCTAT
CTGACCGGTTCCTTCAATCATGGCTCGATGGCGAATGCCATGCCTATGGCGATGGGGGCTGGTTTATCACAACCAGACAG
GCAAGTGATTGCGTTTTGCGGTGATGGCGGTATTTCAATGCTGCTCGGCGATTTAATGACCATTAGCCAATATCAAATTC
CTGTCAAAATCATCATCTTTAACAATCGTTCGTTAGCCATGGTGAAACTTGAAATGCGGGTCGAAGGTTATATGGAGTGG
CAGACGGATATGGTTAATCCTGATTTTATAAAATTAGCAGAGGCGATGAACATTGCTGCATGGGAGGCGACGGAGTCCAA
AGATGTGGAACTCGCACTGGTAAATGGATTCAAGCATAAGGGACCTGCGATAATCAATATTTTTACTGATCCCAATGCCC
TGGCAATGCCACCCACCCTAAACTTCGAACAAGTAAAAGGCTTTGTAGCATCGATGACAAAATTAATGGTCAATGGAAAA
TTTGCCGAAATCGTCGATACCACTAAGAACGATTTAAAATACTTACGGGAGTTATGA

Upstream 100 bases:

>100_bases
CTGGCAGGTTTTGTCTGGAGCAAGATAAACCGCACTTTGTAGTTGCAGGCTAGCTTGGTCATTAATATAGAAATTTTTTA
TCTAAACGTTGGAGCCAATT

Downstream 100 bases:

>100_bases
TAAAACATCATGAAGCAAGCCATTCGATTCACCACAATTGTCCTGGTTATTTTTGTCGCCATCATCGGCATTATACTCTG
GCCTTTAGCGGGTTTGATTA

Product: thiamine pyrophosphate binding domain-containing protein

Products: NA

Alternate protein names: Pyruvate oxidase; POX [H]

Number of amino acids: Translated: 578; Mature: 577

Protein sequence:

>578_residues
MSKNVADHLVEMLVEAGVKRVYAVTGDSLNPVNDAVRRDGRLQWIHVRHEEAGAYAASMEAELNGIGCCMGSSGPGHVHL
VNGLYDANRAENPVIAIASTIHTEKMGLDNFQETRPESLFQDCSKYVFLANTPKQAVNGLQTAIQHAISKKGVAVLGLPG
DVASASLESVHTANKNFYTQPRITPSHKQLQELASVINQHEKVMLFCGHGCRYAVEEVMALAKRLNAPLGYSFRGKIFFE
RKENPYAVGLNGLLGNKSGFEAMHQADVLLMLGTDFPYSEFLPEKCKIIQVDTKPEKLGRRAKVDFGYCGDIKSTLEDLM
PLINAKTSTDFLDKTRSLHQEIEAIYESYVEEKGSEKNIHPEYVAYLVDKLADDDAIFTVDTGMSAVWAARYLKAGANRY
LTGSFNHGSMANAMPMAMGAGLSQPDRQVIAFCGDGGISMLLGDLMTISQYQIPVKIIIFNNRSLAMVKLEMRVEGYMEW
QTDMVNPDFIKLAEAMNIAAWEATESKDVELALVNGFKHKGPAIINIFTDPNALAMPPTLNFEQVKGFVASMTKLMVNGK
FAEIVDTTKNDLKYLREL

Sequences:

>Translated_578_residues
MSKNVADHLVEMLVEAGVKRVYAVTGDSLNPVNDAVRRDGRLQWIHVRHEEAGAYAASMEAELNGIGCCMGSSGPGHVHL
VNGLYDANRAENPVIAIASTIHTEKMGLDNFQETRPESLFQDCSKYVFLANTPKQAVNGLQTAIQHAISKKGVAVLGLPG
DVASASLESVHTANKNFYTQPRITPSHKQLQELASVINQHEKVMLFCGHGCRYAVEEVMALAKRLNAPLGYSFRGKIFFE
RKENPYAVGLNGLLGNKSGFEAMHQADVLLMLGTDFPYSEFLPEKCKIIQVDTKPEKLGRRAKVDFGYCGDIKSTLEDLM
PLINAKTSTDFLDKTRSLHQEIEAIYESYVEEKGSEKNIHPEYVAYLVDKLADDDAIFTVDTGMSAVWAARYLKAGANRY
LTGSFNHGSMANAMPMAMGAGLSQPDRQVIAFCGDGGISMLLGDLMTISQYQIPVKIIIFNNRSLAMVKLEMRVEGYMEW
QTDMVNPDFIKLAEAMNIAAWEATESKDVELALVNGFKHKGPAIINIFTDPNALAMPPTLNFEQVKGFVASMTKLMVNGK
FAEIVDTTKNDLKYLREL
>Mature_577_residues
SKNVADHLVEMLVEAGVKRVYAVTGDSLNPVNDAVRRDGRLQWIHVRHEEAGAYAASMEAELNGIGCCMGSSGPGHVHLV
NGLYDANRAENPVIAIASTIHTEKMGLDNFQETRPESLFQDCSKYVFLANTPKQAVNGLQTAIQHAISKKGVAVLGLPGD
VASASLESVHTANKNFYTQPRITPSHKQLQELASVINQHEKVMLFCGHGCRYAVEEVMALAKRLNAPLGYSFRGKIFFER
KENPYAVGLNGLLGNKSGFEAMHQADVLLMLGTDFPYSEFLPEKCKIIQVDTKPEKLGRRAKVDFGYCGDIKSTLEDLMP
LINAKTSTDFLDKTRSLHQEIEAIYESYVEEKGSEKNIHPEYVAYLVDKLADDDAIFTVDTGMSAVWAARYLKAGANRYL
TGSFNHGSMANAMPMAMGAGLSQPDRQVIAFCGDGGISMLLGDLMTISQYQIPVKIIIFNNRSLAMVKLEMRVEGYMEWQ
TDMVNPDFIKLAEAMNIAAWEATESKDVELALVNGFKHKGPAIINIFTDPNALAMPPTLNFEQVKGFVASMTKLMVNGKF
AEIVDTTKNDLKYLREL

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=486, Percent_Identity=22.6337448559671, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1787096, Length=577, Percent_Identity=42.6343154246101, Blast_Score=483, Evalue=1e-137,
Organism=Escherichia coli, GI1790104, Length=535, Percent_Identity=26.3551401869159, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI87081685, Length=545, Percent_Identity=26.7889908256881, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1786717, Length=533, Percent_Identity=21.5759849906191, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1788716, Length=552, Percent_Identity=23.0072463768116, Blast_Score=96, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17531299, Length=573, Percent_Identity=23.2111692844677, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17531301, Length=575, Percent_Identity=23.4782608695652, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323755, Length=568, Percent_Identity=24.2957746478873, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6320816, Length=493, Percent_Identity=22.920892494929, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI19922626, Length=569, Percent_Identity=23.5500878734622, Blast_Score=98, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.5.1 [H]

Molecular weight: Translated: 63767; Mature: 63636

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNVADHLVEMLVEAGVKRVYAVTGDSLNPVNDAVRRDGRLQWIHVRHEEAGAYAASME
CCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCCEEEEEEEECCCCCCHHCCC
AELNGIGCCMGSSGPGHVHLVNGLYDANRAENPVIAIASTIHTEKMGLDNFQETRPESLF
CCCCCEEEEECCCCCCEEEEEECCCCCCCCCCCEEEEEEHHHHHHCCCCCHHHCCHHHHH
QDCSKYVFLANTPKQAVNGLQTAIQHAISKKGVAVLGLPGDVASASLESVHTANKNFYTQ
HHHHCEEEEECCHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHCCCCEEEC
PRITPSHKQLQELASVINQHEKVMLFCGHGCRYAVEEVMALAKRLNAPLGYSFRGKIFFE
CCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEECEEEEE
RKENPYAVGLNGLLGNKSGFEAMHQADVLLMLGTDFPYSEFLPEKCKIIQVDTKPEKLGR
ECCCCEEEEECCCCCCCCHHHHHHHCCEEEEEECCCCHHHCCCCCCEEEEECCCHHHCCC
RAKVDFGYCGDIKSTLEDLMPLINAKTSTDFLDKTRSLHQEIEAIYESYVEEKGSEKNIH
EECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PEYVAYLVDKLADDDAIFTVDTGMSAVWAARYLKAGANRYLTGSFNHGSMANAMPMAMGA
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHCC
GLSQPDRQVIAFCGDGGISMLLGDLMTISQYQIPVKIIIFNNRSLAMVKLEMRVEGYMEW
CCCCCCCEEEEEECCCCHHHHHHHHHHHHEEECCEEEEEECCCCEEEEEEEEEHHHHHHH
QTDMVNPDFIKLAEAMNIAAWEATESKDVELALVNGFKHKGPAIINIFTDPNALAMPPTL
HHCCCCCHHHHHHHHHHHEEECCCCCCCEEEEEECCCCCCCCEEEEEEECCCCEECCCCC
NFEQVKGFVASMTKLMVNGKFAEIVDTTKNDLKYLREL
CHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure 
SKNVADHLVEMLVEAGVKRVYAVTGDSLNPVNDAVRRDGRLQWIHVRHEEAGAYAASME
CCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCCEEEEEEEECCCCCCHHCCC
AELNGIGCCMGSSGPGHVHLVNGLYDANRAENPVIAIASTIHTEKMGLDNFQETRPESLF
CCCCCEEEEECCCCCCEEEEEECCCCCCCCCCCEEEEEEHHHHHHCCCCCHHHCCHHHHH
QDCSKYVFLANTPKQAVNGLQTAIQHAISKKGVAVLGLPGDVASASLESVHTANKNFYTQ
HHHHCEEEEECCHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHCCCCEEEC
PRITPSHKQLQELASVINQHEKVMLFCGHGCRYAVEEVMALAKRLNAPLGYSFRGKIFFE
CCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEECEEEEE
RKENPYAVGLNGLLGNKSGFEAMHQADVLLMLGTDFPYSEFLPEKCKIIQVDTKPEKLGR
ECCCCEEEEECCCCCCCCHHHHHHHCCEEEEEECCCCHHHCCCCCCEEEEECCCHHHCCC
RAKVDFGYCGDIKSTLEDLMPLINAKTSTDFLDKTRSLHQEIEAIYESYVEEKGSEKNIH
EECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PEYVAYLVDKLADDDAIFTVDTGMSAVWAARYLKAGANRYLTGSFNHGSMANAMPMAMGA
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHCC
GLSQPDRQVIAFCGDGGISMLLGDLMTISQYQIPVKIIIFNNRSLAMVKLEMRVEGYMEW
CCCCCCCEEEEEECCCCHHHHHHHHHHHHEEECCEEEEEECCCCEEEEEEEEEHHHHHHH
QTDMVNPDFIKLAEAMNIAAWEATESKDVELALVNGFKHKGPAIINIFTDPNALAMPPTL
HHCCCCCHHHHHHHHHHHEEECCCCCCCEEEEEECCCCCCCCEEEEEEECCCCEECCCCC
NFEQVKGFVASMTKLMVNGKFAEIVDTTKNDLKYLREL
CHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3016647; 8905232; 9278503; 2663858; 3902830; 8022274 [H]