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The map label for this gene is aglA [H]

Identifier: 126174503

GI number: 126174503

Start: 2656381

End: 2658003

Strand: Reverse

Name: aglA [H]

Synonym: Sbal_2287

Alternate gene names: 126174503

Gene position: 2658003-2656381 (Counterclockwise)

Preceding gene: 126174509

Following gene: 126174502

Centisome position: 51.84

GC content: 46.83

Gene sequence:

>1623_bases
ATGAGTGAGTTAACTTGGTGGCGTGGTGCGGTAATTTACCAAATTTATCCGCGTAGCCTTTTAGATACGAATGGTGATGG
CGTTGGTGATTTGCGTGGCATTATCACTAAGCTGGACTACATTGCGAGCCTCAATGTCGATGCCATCTGGATTTCACCTT
TCTTTAAATCGCCTATGGCAGATTTTGGCTACGACATCAGTGATTACCGCGAAGTTGACCCTCTTTTTGGCACTATGCAT
GATTTCGATGAATTGATTGAAAAAGCCCACGGCTTAGGCATTAAGGTCATTATCGATCAAGTATTAAGTCATACCTCTGA
TCAGCATGCGTGGTTTGTTGAAAGCCGCGAGAGCCGTACGAATCCCAAAGCGGATTGGTACGTGTGGGCCGATCCTAAGG
AAGACGGCACTGCGCCTAACAACTGGCTCGCGATTTTTGGCGGTTGTGCTTGGGAATGGGAACCGCGCAGACAACAATAC
TATTTACATAACTTCCTGCGTAGCCAGCCCGATATCAACTTCCATAATCCTGACGTGCGCCAAGCTGTGCTGGATAATGT
CGAGTTCTGGCTTAAAAAAGGCGTTGATGGCTTTCGCTTAGATGCGATTACCTTCTGTTATCACGATGAACAGCTCAGGG
ACAATCCGGCAAAACCTAAGGATAAACGTCAGGGCCGAGGTTTCAGCGAAGATAACCCCTACGCCTATCAATATCATTAC
TACAATAATGACCGTCCGCAAACTATCGCGTTTATCGAAGACTTACGCCAACTCATTAATCGTTATCCTGGTGCGGTGAC
CTTAGGTGAAGTGTCTGCAGAAGACTCTTTAGCTGTGATGGCGGCTTACACTAAGGGTGAAGATCGCCTGCACATGGCCT
ACAGTTTTGAGCTATTAACCGACGATTACAGTGCTGCTTATATTCGCCAAACCGTAGAAGCATTAGAAGCGAGCATAGGT
GATGGGTGGCCTTGTTGGGCTATCGGTAATCATGATGTGCAGCGCGTGGCGAGTCGTTGGGGGCAGGGCAAACAGAATTC
AGACATGGTCAAAATACTCAATGCCATGCTAAGTTCACTGCGCGGTAGTGTGTGTAGTTATCAAGGTGAAGAACTTGGTT
TAACCGAAGCGCCGATTGAATACCATGAGTTGCAAGATCCCTTTGGCAAAACCTTCTGGCCTATGTTTAAAGGCCGTGAT
GGTTGCCGTACGCCAATGCCATGGCAACAAGGCGCCGATTTTAGCGGTTTTAGTGAGGTTTCACCCTGGTTGCCGATCGC
CCCTGCGCATCAAGCGTTAGCGGTGGATGTACAAGAATCAGATGCAGATTCCATGCTTAACGGTTATCGACGTTTTCTTG
CTTGGCGTAAATTACATCCAGTGTTGGTTGAAGGCGATATCCAATTTGTGGATACCAACGAGCCCATCTTGGCCTTCATC
CGCACCTTAGGAGAGCTCAAAATATTCGTCGGATTCAACTTAGGCGATACCAATGTTGAGTTGGATTTATCACAGTTGGA
ATTGGGTGAAATATTAACGGGACACGGCCTGAAAATCGCACAAATAGAAAACAATCAGCTGAAGTTTTCGGCATTTGCCA
GCTTTTATGCAGTTTTAAACTGA

Upstream 100 bases:

>100_bases
AATAAGCCGCGATACCTTTACTCTGCAGTAACAAAACGATAACTGTTTGCATGAGTGTGTTAGGGAATGATCGCTGTAGA
ATAACGATAAAGGGGCAACA

Downstream 100 bases:

>100_bases
AAATGATTCATTTTTATTCGGCTGAGGTGTTATATACCCAAGCCCACTATTCGACGTAAAGCCGCCCACGGGCACACTGG
GGAATGTAACCTTACTGAGT

Product: alpha amylase catalytic region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MSELTWWRGAVIYQIYPRSLLDTNGDGVGDLRGIITKLDYIASLNVDAIWISPFFKSPMADFGYDISDYREVDPLFGTMH
DFDELIEKAHGLGIKVIIDQVLSHTSDQHAWFVESRESRTNPKADWYVWADPKEDGTAPNNWLAIFGGCAWEWEPRRQQY
YLHNFLRSQPDINFHNPDVRQAVLDNVEFWLKKGVDGFRLDAITFCYHDEQLRDNPAKPKDKRQGRGFSEDNPYAYQYHY
YNNDRPQTIAFIEDLRQLINRYPGAVTLGEVSAEDSLAVMAAYTKGEDRLHMAYSFELLTDDYSAAYIRQTVEALEASIG
DGWPCWAIGNHDVQRVASRWGQGKQNSDMVKILNAMLSSLRGSVCSYQGEELGLTEAPIEYHELQDPFGKTFWPMFKGRD
GCRTPMPWQQGADFSGFSEVSPWLPIAPAHQALAVDVQESDADSMLNGYRRFLAWRKLHPVLVEGDIQFVDTNEPILAFI
RTLGELKIFVGFNLGDTNVELDLSQLELGEILTGHGLKIAQIENNQLKFSAFASFYAVLN

Sequences:

>Translated_540_residues
MSELTWWRGAVIYQIYPRSLLDTNGDGVGDLRGIITKLDYIASLNVDAIWISPFFKSPMADFGYDISDYREVDPLFGTMH
DFDELIEKAHGLGIKVIIDQVLSHTSDQHAWFVESRESRTNPKADWYVWADPKEDGTAPNNWLAIFGGCAWEWEPRRQQY
YLHNFLRSQPDINFHNPDVRQAVLDNVEFWLKKGVDGFRLDAITFCYHDEQLRDNPAKPKDKRQGRGFSEDNPYAYQYHY
YNNDRPQTIAFIEDLRQLINRYPGAVTLGEVSAEDSLAVMAAYTKGEDRLHMAYSFELLTDDYSAAYIRQTVEALEASIG
DGWPCWAIGNHDVQRVASRWGQGKQNSDMVKILNAMLSSLRGSVCSYQGEELGLTEAPIEYHELQDPFGKTFWPMFKGRD
GCRTPMPWQQGADFSGFSEVSPWLPIAPAHQALAVDVQESDADSMLNGYRRFLAWRKLHPVLVEGDIQFVDTNEPILAFI
RTLGELKIFVGFNLGDTNVELDLSQLELGEILTGHGLKIAQIENNQLKFSAFASFYAVLN
>Mature_539_residues
SELTWWRGAVIYQIYPRSLLDTNGDGVGDLRGIITKLDYIASLNVDAIWISPFFKSPMADFGYDISDYREVDPLFGTMHD
FDELIEKAHGLGIKVIIDQVLSHTSDQHAWFVESRESRTNPKADWYVWADPKEDGTAPNNWLAIFGGCAWEWEPRRQQYY
LHNFLRSQPDINFHNPDVRQAVLDNVEFWLKKGVDGFRLDAITFCYHDEQLRDNPAKPKDKRQGRGFSEDNPYAYQYHYY
NNDRPQTIAFIEDLRQLINRYPGAVTLGEVSAEDSLAVMAAYTKGEDRLHMAYSFELLTDDYSAAYIRQTVEALEASIGD
GWPCWAIGNHDVQRVASRWGQGKQNSDMVKILNAMLSSLRGSVCSYQGEELGLTEAPIEYHELQDPFGKTFWPMFKGRDG
CRTPMPWQQGADFSGFSEVSPWLPIAPAHQALAVDVQESDADSMLNGYRRFLAWRKLHPVLVEGDIQFVDTNEPILAFIR
TLGELKIFVGFNLGDTNVELDLSQLELGEILTGHGLKIAQIENNQLKFSAFASFYAVLN

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=539, Percent_Identity=33.2096474953618, Blast_Score=282, Evalue=5e-76,
Organism=Escherichia coli, GI1790687, Length=511, Percent_Identity=35.2250489236791, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1786604, Length=495, Percent_Identity=23.4343434343434, Blast_Score=97, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI32565753, Length=514, Percent_Identity=23.3463035019455, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI25147709, Length=560, Percent_Identity=22.6785714285714, Blast_Score=97, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321731, Length=558, Percent_Identity=34.7670250896057, Blast_Score=290, Evalue=5e-79,
Organism=Saccharomyces cerevisiae, GI6319776, Length=558, Percent_Identity=35.3046594982079, Blast_Score=290, Evalue=6e-79,
Organism=Saccharomyces cerevisiae, GI6322245, Length=556, Percent_Identity=33.4532374100719, Blast_Score=283, Evalue=4e-77,
Organism=Saccharomyces cerevisiae, GI6321726, Length=555, Percent_Identity=32.972972972973, Blast_Score=277, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6324416, Length=553, Percent_Identity=32.7305605786618, Blast_Score=269, Evalue=7e-73,
Organism=Saccharomyces cerevisiae, GI6322241, Length=553, Percent_Identity=32.7305605786618, Blast_Score=269, Evalue=9e-73,
Organism=Saccharomyces cerevisiae, GI6322021, Length=553, Percent_Identity=32.7305605786618, Blast_Score=269, Evalue=9e-73,
Organism=Drosophila melanogaster, GI24583747, Length=527, Percent_Identity=37.9506641366224, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24583749, Length=527, Percent_Identity=37.9506641366224, Blast_Score=325, Evalue=4e-89,
Organism=Drosophila melanogaster, GI24586587, Length=522, Percent_Identity=36.9731800766284, Blast_Score=318, Evalue=5e-87,
Organism=Drosophila melanogaster, GI24586591, Length=520, Percent_Identity=36.1538461538462, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI24583745, Length=529, Percent_Identity=36.2948960302458, Blast_Score=311, Evalue=9e-85,
Organism=Drosophila melanogaster, GI24586593, Length=500, Percent_Identity=35.8, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24586599, Length=522, Percent_Identity=34.8659003831418, Blast_Score=305, Evalue=4e-83,
Organism=Drosophila melanogaster, GI24586589, Length=534, Percent_Identity=34.8314606741573, Blast_Score=305, Evalue=7e-83,
Organism=Drosophila melanogaster, GI24586597, Length=551, Percent_Identity=34.8457350272232, Blast_Score=301, Evalue=9e-82,
Organism=Drosophila melanogaster, GI221330053, Length=555, Percent_Identity=33.1531531531532, Blast_Score=298, Evalue=9e-81,
Organism=Drosophila melanogaster, GI45549022, Length=560, Percent_Identity=34.2857142857143, Blast_Score=296, Evalue=3e-80,
Organism=Drosophila melanogaster, GI281360393, Length=443, Percent_Identity=33.1828442437923, Blast_Score=235, Evalue=5e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.20 [H]

Molecular weight: Translated: 61579; Mature: 61447

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELTWWRGAVIYQIYPRSLLDTNGDGVGDLRGIITKLDYIASLNVDAIWISPFFKSPMA
CCCCCCCCCEEEEEECCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECHHHCCCHH
DFGYDISDYREVDPLFGTMHDFDELIEKAHGLGIKVIIDQVLSHTSDQHAWFVESRESRT
HHCCCHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECCCCCC
NPKADWYVWADPKEDGTAPNNWLAIFGGCAWEWEPRRQQYYLHNFLRSQPDINFHNPDVR
CCCCCEEEECCCCCCCCCCCCEEEEEECEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHH
QAVLDNVEFWLKKGVDGFRLDAITFCYHDEQLRDNPAKPKDKRQGRGFSEDNPYAYQYHY
HHHHHHHHHHHHCCCCCEEEEEEEEEECCHHHHCCCCCCCHHHCCCCCCCCCCEEEEEEE
YNNDRPQTIAFIEDLRQLINRYPGAVTLGEVSAEDSLAVMAAYTKGEDRLHMAYSFELLT
ECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEECCCCEEEEEEEEEEEC
DDYSAAYIRQTVEALEASIGDGWPCWAIGNHDVQRVASRWGQGKQNSDMVKILNAMLSSL
CCCHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
RGSVCSYQGEELGLTEAPIEYHELQDPFGKTFWPMFKGRDGCRTPMPWQQGADFSGFSEV
CCCHHCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
SPWLPIAPAHQALAVDVQESDADSMLNGYRRFLAWRKLHPVLVEGDIQFVDTNEPILAFI
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCHHHHHH
RTLGELKIFVGFNLGDTNVELDLSQLELGEILTGHGLKIAQIENNQLKFSAFASFYAVLN
HHHCCEEEEEEECCCCCCEEEEHHHHHHHHHHCCCCEEEEEECCCCEEHHHHHHHHHHCC
>Mature Secondary Structure 
SELTWWRGAVIYQIYPRSLLDTNGDGVGDLRGIITKLDYIASLNVDAIWISPFFKSPMA
CCCCCCCCEEEEEECCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECHHHCCCHH
DFGYDISDYREVDPLFGTMHDFDELIEKAHGLGIKVIIDQVLSHTSDQHAWFVESRESRT
HHCCCHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECCCCCC
NPKADWYVWADPKEDGTAPNNWLAIFGGCAWEWEPRRQQYYLHNFLRSQPDINFHNPDVR
CCCCCEEEECCCCCCCCCCCCEEEEEECEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHH
QAVLDNVEFWLKKGVDGFRLDAITFCYHDEQLRDNPAKPKDKRQGRGFSEDNPYAYQYHY
HHHHHHHHHHHHCCCCCEEEEEEEEEECCHHHHCCCCCCCHHHCCCCCCCCCCEEEEEEE
YNNDRPQTIAFIEDLRQLINRYPGAVTLGEVSAEDSLAVMAAYTKGEDRLHMAYSFELLT
ECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEECCCCEEEEEEEEEEEC
DDYSAAYIRQTVEALEASIGDGWPCWAIGNHDVQRVASRWGQGKQNSDMVKILNAMLSSL
CCCHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
RGSVCSYQGEELGLTEAPIEYHELQDPFGKTFWPMFKGRDGCRTPMPWQQGADFSGFSEV
CCCHHCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
SPWLPIAPAHQALAVDVQESDADSMLNGYRRFLAWRKLHPVLVEGDIQFVDTNEPILAFI
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCHHHHHH
RTLGELKIFVGFNLGDTNVELDLSQLELGEILTGHGLKIAQIENNQLKFSAFASFYAVLN
HHHCCEEEEEEECCCCCCEEEEHHHHHHHHHHCCCCEEEEEECCCCEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10400573; 11481430 [H]