Click here to switch to the map view.

The map label for this gene is pleD [H]

Identifier: 126174498

GI number: 126174498

Start: 2648740

End: 2649717

Strand: Direct

Name: pleD [H]

Synonym: Sbal_2282

Alternate gene names: 126174498

Gene position: 2648740-2649717 (Clockwise)

Preceding gene: 126174496

Following gene: 126174499

Centisome position: 51.66

GC content: 39.98

Gene sequence:

>978_bases
ATGGAACTAAAGGAACAGTATTCTATTCCTCGTATCCCAGCGATACTTTTTATGCTTGCCGCAATACTGACACTGTGTAC
CTTCGTTATTTACCGTTACATCCACCAAGACTACATCCTTACTTTTGTCTTTGGCATCGCCTTATTACCCTTATGCATTA
CATTAATCTTAGAGATAAGGCGTAAATCGAATTTACTCCAGAAGCAACTGACGCTACTCATAATCTGTGGGGCCATCATT
TTTAGCTGTTATGAAATAGGCTATAAAGGATTAATTTATATTTCTCCAACAGTATTTATCTTCTTTTTCCTTTTCAATCT
AAAATCAAGCCTAATATTGAGCACCCTCTATGCCTGTATCAGTTTAGTGAGTGCATTACAGGTAGAGTCAGTCGAATTAG
TTCTGAGGTTCTCAGTCGCCACCTTTGATTGCATTATCTTTGCCGCTATTTTTGCGCATATCGTTAATAAACAGAGACAA
GCTCTAGTCCATTTAGCCAATACGGATGAGCTCACAGGAGCACTCAACCGCAAACAACTCAAAACTAAGCTTGAACTGAG
CGTCTATGAGTATCAGCAACAGCAAATCCCTGCTAGTTTACTCTTGATAGATATCGATTTTTTCAAAATGGTGAACGATA
ATTTTGGTCATATCATGGGCGACCAATTACTCAATAAATTCAGTCAACACATCCAAAAAAAGATCAGAAAAGACGACTTA
TTATTCCGTTTCGGCGGAGAAGAATTTCTGATCTTGCTACCCAATACTCAATTTGAGTCGGCTGCCATTGTCGCCGAAGA
CCTTAGACGAAGTATTGAAGAACAACACTTTCTCGACCAACACCCCCAAGTCACTTGCAGTATTGGAGTTGCTGATTTAC
GTCAAGCTGACACCTTAGACACTTGGCTTAAAACCTGCGATAACCTGCTGTATACTGCTAAAAATGCAGGACGCAATTGT
GTTGTGGCTCGCGCGTAA

Upstream 100 bases:

>100_bases
TGAGTCGCTACAAGTTCTAGTAAAAACTGCCATTATATGCGCTGTAATAATGACTTGTGAGCTAAACTAGCCTCATTAAT
GCTTACTAACGGTAGTAAAT

Downstream 100 bases:

>100_bases
TCATCCGTCTGACTATAGCGACGAATGAGTGATGCAAAGAATCACTATCTAACCCCACAAAAATCAAAATAAACTCACCA
AATGCACAAGCCCTTATTTG

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MELKEQYSIPRIPAILFMLAAILTLCTFVIYRYIHQDYILTFVFGIALLPLCITLILEIRRKSNLLQKQLTLLIICGAII
FSCYEIGYKGLIYISPTVFIFFFLFNLKSSLILSTLYACISLVSALQVESVELVLRFSVATFDCIIFAAIFAHIVNKQRQ
ALVHLANTDELTGALNRKQLKTKLELSVYEYQQQQIPASLLLIDIDFFKMVNDNFGHIMGDQLLNKFSQHIQKKIRKDDL
LFRFGGEEFLILLPNTQFESAAIVAEDLRRSIEEQHFLDQHPQVTCSIGVADLRQADTLDTWLKTCDNLLYTAKNAGRNC
VVARA

Sequences:

>Translated_325_residues
MELKEQYSIPRIPAILFMLAAILTLCTFVIYRYIHQDYILTFVFGIALLPLCITLILEIRRKSNLLQKQLTLLIICGAII
FSCYEIGYKGLIYISPTVFIFFFLFNLKSSLILSTLYACISLVSALQVESVELVLRFSVATFDCIIFAAIFAHIVNKQRQ
ALVHLANTDELTGALNRKQLKTKLELSVYEYQQQQIPASLLLIDIDFFKMVNDNFGHIMGDQLLNKFSQHIQKKIRKDDL
LFRFGGEEFLILLPNTQFESAAIVAEDLRRSIEEQHFLDQHPQVTCSIGVADLRQADTLDTWLKTCDNLLYTAKNAGRNC
VVARA
>Mature_325_residues
MELKEQYSIPRIPAILFMLAAILTLCTFVIYRYIHQDYILTFVFGIALLPLCITLILEIRRKSNLLQKQLTLLIICGAII
FSCYEIGYKGLIYISPTVFIFFFLFNLKSSLILSTLYACISLVSALQVESVELVLRFSVATFDCIIFAAIFAHIVNKQRQ
ALVHLANTDELTGALNRKQLKTKLELSVYEYQQQQIPASLLLIDIDFFKMVNDNFGHIMGDQLLNKFSQHIQKKIRKDDL
LFRFGGEEFLILLPNTQFESAAIVAEDLRRSIEEQHFLDQHPQVTCSIGVADLRQADTLDTWLKTCDNLLYTAKNAGRNC
VVARA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=174, Percent_Identity=39.6551724137931, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1787262, Length=205, Percent_Identity=36.5853658536585, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786584, Length=166, Percent_Identity=35.5421686746988, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI1787802, Length=192, Percent_Identity=33.3333333333333, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI145693134, Length=161, Percent_Identity=33.5403726708075, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI87082007, Length=160, Percent_Identity=36.25, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788381, Length=177, Percent_Identity=31.0734463276836, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87081974, Length=147, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787541, Length=168, Percent_Identity=31.547619047619, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787816, Length=156, Percent_Identity=28.2051282051282, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787056, Length=236, Percent_Identity=26.271186440678, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1788085, Length=164, Percent_Identity=32.3170731707317, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 37146; Mature: 37146

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKEQYSIPRIPAILFMLAAILTLCTFVIYRYIHQDYILTFVFGIALLPLCITLILEIR
CCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKSNLLQKQLTLLIICGAIIFSCYEIGYKGLIYISPTVFIFFFLFNLKSSLILSTLYACI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
SLVSALQVESVELVLRFSVATFDCIIFAAIFAHIVNKQRQALVHLANTDELTGALNRKQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
KTKLELSVYEYQQQQIPASLLLIDIDFFKMVNDNFGHIMGDQLLNKFSQHIQKKIRKDDL
HHHHHHHHHHHHHHHCCHHHEEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCE
LFRFGGEEFLILLPNTQFESAAIVAEDLRRSIEEQHFLDQHPQVTCSIGVADLRQADTLD
EEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHH
TWLKTCDNLLYTAKNAGRNCVVARA
HHHHHHHHHHHHHCCCCCCEEEECC
>Mature Secondary Structure
MELKEQYSIPRIPAILFMLAAILTLCTFVIYRYIHQDYILTFVFGIALLPLCITLILEIR
CCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKSNLLQKQLTLLIICGAIIFSCYEIGYKGLIYISPTVFIFFFLFNLKSSLILSTLYACI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
SLVSALQVESVELVLRFSVATFDCIIFAAIFAHIVNKQRQALVHLANTDELTGALNRKQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
KTKLELSVYEYQQQQIPASLLLIDIDFFKMVNDNFGHIMGDQLLNKFSQHIQKKIRKDDL
HHHHHHHHHHHHHHHCCHHHEEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCE
LFRFGGEEFLILLPNTQFESAAIVAEDLRRSIEEQHFLDQHPQVTCSIGVADLRQADTLD
EEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHH
TWLKTCDNLLYTAKNAGRNCVVARA
HHHHHHHHHHHHHCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA