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The map label for this gene is pdhA [H]

Identifier: 126174438

GI number: 126174438

Start: 2570628

End: 2571806

Strand: Reverse

Name: pdhA [H]

Synonym: Sbal_2222

Alternate gene names: 126174438

Gene position: 2571806-2570628 (Counterclockwise)

Preceding gene: 126174439

Following gene: 126174437

Centisome position: 50.16

GC content: 48.69

Gene sequence:

>1179_bases
ATGAGCAAAGCAACACTCAACACTGATACAGTGCACCGTGTCGGCTTCTTGGATAAGGCATCGCTACACATTCCTATCCT
TCGAATTCTACAAGCCGACGGCACCACTTATGAAACCGCGGTTTTGCCTGTGATAGATGAAACCTTAGCCACTAAAATTT
ACGATACCTGCGTGTTCACTCGGGTACTCGATGAACGTATGTTAGGTGCCCAACGTCAAGGGCGCATCAGCTTCTATATG
ACCTGTACGGGTGAAGAAGCGGCGATTGTCGGCAGTGTAGCCGCACTCGTTCAAGAAGACGTTATCCTCGCGCAATACCG
TGAGCATGCGGCGCTGCGTTATCGCGGCTTTACCACTGAACAGTTTATGAATCAGATGTTCAGTAACGAGAAAGATCTTG
GTAAAGGTCGCCAAATGCCAATCCATTACGGCAGTGCGGCATTGCATTATCAAACGATTTCTTCACCCCTTGCGACACAA
ATCCCGCAGGCGACGGGTGTGGGCTACAGCTTAAAAATGCAAGGTAAGCGCAAAGTCGCGGTTTGCTATTTTGGCGAAGG
CGCTGCATCGGAAGGGGACTTCCATGCCGGTCTAAACATGGCCGCAGTATTGAAGTGTCCAGTGATTTTCTTCTGTCGTA
ACAATGGCTACGCCATTTCAACCCCAACTGAAGAGCAGTTTGCAGGTAACGGTATTGCGAGTCGCGGTGTGGGTTATGGC
ATGCACACGATTCGTGTTGACGGCAATGACATGCTGGCCGTGTTAGCGGCAACCCAACAAGCCCGCGCCTATGCGATTGA
GCATAATGCCCCTGTGCTGATTGAAGCCATGACTTACCGTCTCGGCGCGCATTCATCATCAGACGATCCATCTGGTTATC
GCTCGAAAGATGAAGAAGCCAAGTGGCAGCAACACGATCCGGTTAAACGCTTCAAATTATGGCTTATCAACAAAGGTTGG
TTAGCTGAAGCGGATGATGTGAAACTACATGAGAAATACCGTGAAGAAGTATTGGCTGCGGTAAAAGTGGCTGAAAAAAT
TCCTGTGCCTATGCTGGATGAAATTATTGAAGATGTGTACGACAAGCCGACGCCAGTGCTGAGAAAGCAACTCGCCGATC
TTAAAGAACATATCAAAAAATATCCGCAAGCCTATCCAAAAAGTGCAGGGAGACTATAA

Upstream 100 bases:

>100_bases
TTGATAGGTAGTTTACGTTAACGTAATGTTCGTCGGGCATATTCGTATAAACAGACGCATCAAAACGCTTTGATGCGTAA
TGAGATAACAAGAGCACAAT

Downstream 100 bases:

>100_bases
GCCGTGGCTGAAATGAATATGTTACAGGCCGTCAATGAGGCCTTGTCGATCGCCATGCAAGCCGATGAGCGCATGGTGGT
TTTTGGTGAAGATGTGGGTC

Product: 3-methyl-2-oxobutanoate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MSKATLNTDTVHRVGFLDKASLHIPILRILQADGTTYETAVLPVIDETLATKIYDTCVFTRVLDERMLGAQRQGRISFYM
TCTGEEAAIVGSVAALVQEDVILAQYREHAALRYRGFTTEQFMNQMFSNEKDLGKGRQMPIHYGSAALHYQTISSPLATQ
IPQATGVGYSLKMQGKRKVAVCYFGEGAASEGDFHAGLNMAAVLKCPVIFFCRNNGYAISTPTEEQFAGNGIASRGVGYG
MHTIRVDGNDMLAVLAATQQARAYAIEHNAPVLIEAMTYRLGAHSSSDDPSGYRSKDEEAKWQQHDPVKRFKLWLINKGW
LAEADDVKLHEKYREEVLAAVKVAEKIPVPMLDEIIEDVYDKPTPVLRKQLADLKEHIKKYPQAYPKSAGRL

Sequences:

>Translated_392_residues
MSKATLNTDTVHRVGFLDKASLHIPILRILQADGTTYETAVLPVIDETLATKIYDTCVFTRVLDERMLGAQRQGRISFYM
TCTGEEAAIVGSVAALVQEDVILAQYREHAALRYRGFTTEQFMNQMFSNEKDLGKGRQMPIHYGSAALHYQTISSPLATQ
IPQATGVGYSLKMQGKRKVAVCYFGEGAASEGDFHAGLNMAAVLKCPVIFFCRNNGYAISTPTEEQFAGNGIASRGVGYG
MHTIRVDGNDMLAVLAATQQARAYAIEHNAPVLIEAMTYRLGAHSSSDDPSGYRSKDEEAKWQQHDPVKRFKLWLINKGW
LAEADDVKLHEKYREEVLAAVKVAEKIPVPMLDEIIEDVYDKPTPVLRKQLADLKEHIKKYPQAYPKSAGRL
>Mature_391_residues
SKATLNTDTVHRVGFLDKASLHIPILRILQADGTTYETAVLPVIDETLATKIYDTCVFTRVLDERMLGAQRQGRISFYMT
CTGEEAAIVGSVAALVQEDVILAQYREHAALRYRGFTTEQFMNQMFSNEKDLGKGRQMPIHYGSAALHYQTISSPLATQI
PQATGVGYSLKMQGKRKVAVCYFGEGAASEGDFHAGLNMAAVLKCPVIFFCRNNGYAISTPTEEQFAGNGIASRGVGYGM
HTIRVDGNDMLAVLAATQQARAYAIEHNAPVLIEAMTYRLGAHSSSDDPSGYRSKDEEAKWQQHDPVKRFKLWLINKGWL
AEADDVKLHEKYREEVLAAVKVAEKIPVPMLDEIIEDVYDKPTPVLRKQLADLKEHIKKYPQAYPKSAGRL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11386135, Length=364, Percent_Identity=50, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI258645172, Length=364, Percent_Identity=50, Blast_Score=364, Evalue=1e-101,
Organism=Homo sapiens, GI291084742, Length=290, Percent_Identity=30.6896551724138, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI4505685, Length=290, Percent_Identity=30.6896551724138, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI4885543, Length=285, Percent_Identity=28.7719298245614, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI291084744, Length=283, Percent_Identity=30.3886925795053, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI291084757, Length=291, Percent_Identity=27.4914089347079, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI86563357, Length=365, Percent_Identity=50.958904109589, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI86563355, Length=365, Percent_Identity=50.958904109589, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17536047, Length=303, Percent_Identity=30.03300330033, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32564172, Length=303, Percent_Identity=30.03300330033, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321026, Length=315, Percent_Identity=29.5238095238095, Blast_Score=128, Evalue=1e-30,
Organism=Drosophila melanogaster, GI21355903, Length=366, Percent_Identity=45.9016393442623, Blast_Score=344, Evalue=5e-95,
Organism=Drosophila melanogaster, GI24639746, Length=301, Percent_Identity=31.5614617940199, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24639740, Length=301, Percent_Identity=31.5614617940199, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24639744, Length=301, Percent_Identity=31.5614617940199, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI28571106, Length=301, Percent_Identity=31.5614617940199, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24639748, Length=300, Percent_Identity=30.3333333333333, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017
- InterPro:   IPR017596 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 43551; Mature: 43420

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKATLNTDTVHRVGFLDKASLHIPILRILQADGTTYETAVLPVIDETLATKIYDTCVFT
CCCCCCCCCHHHHHCCCCCCCCCHHHHHEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHH
RVLDERMLGAQRQGRISFYMTCTGEEAAIVGSVAALVQEDVILAQYREHAALRYRGFTTE
HHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHH
QFMNQMFSNEKDLGKGRQMPIHYGSAALHYQTISSPLATQIPQATGVGYSLKMQGKRKVA
HHHHHHHCCCCCCCCCCCCCEEECCEEEEHHHHCCHHHHHCCCCCCCCEEEEECCCCEEE
VCYFGEGAASEGDFHAGLNMAAVLKCPVIFFCRNNGYAISTPTEEQFAGNGIASRGVGYG
EEEECCCCCCCCCCCCCCCHHHHHHCCEEEEECCCCEEECCCCHHHHCCCCCCCCCCCCC
MHTIRVDGNDMLAVLAATQQARAYAIEHNAPVLIEAMTYRLGAHSSSDDPSGYRSKDEEA
EEEEEECCCCEEEEEEHHHHHHEEEEECCCCEEEEEHHHHHCCCCCCCCCCCCCCCCCCC
KWQQHDPVKRFKLWLINKGWLAEADDVKLHEKYREEVLAAVKVAEKIPVPMLDEIIEDVY
CCCCCCCHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
DKPTPVLRKQLADLKEHIKKYPQAYPKSAGRL
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SKATLNTDTVHRVGFLDKASLHIPILRILQADGTTYETAVLPVIDETLATKIYDTCVFT
CCCCCCCCHHHHHCCCCCCCCCHHHHHEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHH
RVLDERMLGAQRQGRISFYMTCTGEEAAIVGSVAALVQEDVILAQYREHAALRYRGFTTE
HHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHH
QFMNQMFSNEKDLGKGRQMPIHYGSAALHYQTISSPLATQIPQATGVGYSLKMQGKRKVA
HHHHHHHCCCCCCCCCCCCCEEECCEEEEHHHHCCHHHHHCCCCCCCCEEEEECCCCEEE
VCYFGEGAASEGDFHAGLNMAAVLKCPVIFFCRNNGYAISTPTEEQFAGNGIASRGVGYG
EEEECCCCCCCCCCCCCCCHHHHHHCCEEEEECCCCEEECCCCHHHHCCCCCCCCCCCCC
MHTIRVDGNDMLAVLAATQQARAYAIEHNAPVLIEAMTYRLGAHSSSDDPSGYRSKDEEA
EEEEEECCCCEEEEEEHHHHHHEEEEECCCCEEEEEHHHHHCCCCCCCCCCCCCCCCCCC
KWQQHDPVKRFKLWLINKGWLAEADDVKLHEKYREEVLAAVKVAEKIPVPMLDEIIEDVY
CCCCCCCHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
DKPTPVLRKQLADLKEHIKKYPQAYPKSAGRL
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA