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The map label for this gene is btuB [H]

Identifier: 126174210

GI number: 126174210

Start: 2303076

End: 2305679

Strand: Reverse

Name: btuB [H]

Synonym: Sbal_1986

Alternate gene names: 126174210

Gene position: 2305679-2303076 (Counterclockwise)

Preceding gene: 126174212

Following gene: 126174209

Centisome position: 44.97

GC content: 47.81

Gene sequence:

>2604_bases
ATGAGAAGTTCTGTATTTTTGTGCGCTGTTTTTGGGTTGGCCTTGTCGCCCGTTGCTTTAGCTGTAGCCAATGAACTAGA
AAAAACAACTGAGCAACCTGCCGCAGATGCACCGGTCGAGCGAGTCGCAGTCACAGGTTCACGCCTAAAAGGGGTGGATA
TGGAAGGCGCCAATCCACTGCAAAGTTTTACCCAGGATGACATCACTAAACGCGGTTACGAGAGTGTGAGTGAGTTTCTA
CGCGATCTCCCACAAGCTTCAAGTGCGGGGACTTTCTCGGAAACTGGGGGTGTCGGTGGAGCCGATGGAGCACCAGCTGG
CGCATCCGGTGTCAGTTTGCGCGGATTGGGTTCAAGCTCAACCTTAGTGCTAGTCAATGGCCGAAGAGTCGCCGTTGATT
CATTCTCTAATGGTTCTGACTCGTTTGTGAACGTCAATTCAATCCCTTTATCTGCCATTGAACGCATCGATGTGTTAACC
GATGGTGCATCTTCCATCTATGGTTCGGATGCCATTGCCGGGGTGATTAACTTTATCCTCCGTAAGGATTTTGTCGGGCA
TGAGCTCAGTGCCCAATATGGGGATGATACTTCCGCTCACGATGCCAGCCGTAAGAATCTGACCTACGCTGGCGGCTTTG
CCACAGAAAACAGCAATACTACAGTTGTAGTCGATTATGCGACGCGAAACGCCATCTTTAATAGCGATAGACCTGTTAAT
GTTACCTTTGAATCCAGCACTCGTATGACCATAGATGGTGAGGATTACGCTGAGTCTTGGTGTGGCGACGATACTCAAAA
AAGTGGTACACGTTGCAATTATGACTATGTTATTCAACGCGCTATCCAACCTGACTTTGACAATATAGGCACGACCTTTA
ATCATATTTATCGTCTAGATAATGGATTGGAGTTTTTTGCTGAAGGCATGTATCAAAAGAATACCGGTTCAGCCTATGAG
GCGCCTGCGGGATATACAGTGGATGTTGCTGGTGATTCTGCCAACATTCCCCAGTATGTTCACGACATTGACATGCTAGA
TGGCAGTGTCAGCGACGTTATCACTGTTCGAACTCGTTTCCCTGAGCGTCGAGTACAGGAATATGACACTCAGAGCTATC
GAGTCCTCGCTGGACTGCGCGGTGAACTTGCCGACTGGAGCTGGGAAACCGCGGCGAGTTATGGCAAATCTACAAACGAA
ATCACCAATGTTGCTGGCTATATGAATGCCACTAAACTGGATGCCGCCGTCGCGGCGGATGAATTTAATCCGTTCAATTT
AGGCCGTGATAGTAGCGCGGCACAAATTGCTGCCCTGCGCGATCCCGGCATGCGTAAAGGTGAATCAGAGGTGTTGTCGC
TGGATTTTAATATAAGTGGCGATTTGTTTGCATTGCCCGCAGGTACAGTAAGCTCGGTTTTTGGTGGAGAGCTGCGAAAA
GAGCAAATATTTGATAGGCCATCCATCAACGCCGTTAATAACGAAATTATCGGCTTAGGCGCAAGCGATGCCGAAGCAGA
CCGGACGCAATATGCTGTGTATGGCGAGTTTAATTTCCCCATTACCGACACCTTAGATGTGATCGCAGCTTTACGTTACG
ACCACTATAGTGACTTTGGTGGCGATGTTAACCCTAAAATATCAATGCGTTATCGCCCACTTGAGGATTTAGTATTAAGG
GCATCATGGAGTACAGGTTTTAGAGCCCCTTCCTTATCCCAATTAGGCGCAGGGACGTCGCTCAGCAGCCAGTATATCAA
TTGCGGTTCAGCTGAACCTTACAATGCCTTATGCGGTACTGCTGGCGCCGAGTTTGGCGAACTCGAGTTTGACCAAGAAA
CCATAGGCAATAAGGATTTATCCGCAGAAAAGTCTGCCGCCATTAACGTTGGCGTCTCTTGGAACCTCACCGAAGCACTA
CAATTGACTGTCGATTACTGGCGCTATGAGCACGATGATATTGTTGATATTGATGCCAACACCACCTTAGACGCCTGTAT
TAAAGACAGTACAAAAATCGTGACTAGCGTTGCAGAACTGGGGGATGAATTCGGCTGTGTTGTTGATGGCCAAGGGGATA
TCAGCTTCTTACGCACTGGCTATTTTAACGTCGGCAATCAAAATACTGATGGCATCGACTACAAGCTAGATTATCGCTTA
GACACTGAAACCGCAGGTGAATTTAAGTTTTATGTTGCGGGCACTGAAACCCTGTCGTTTGAAAGACAAGTTACACCTTC
AACCCCAGTGGAAGATTTATTAGGGCGGTTAAGTGGCGCCTCAGAAATTGCTCGTCCTGAAAGCGTGCTTGATCTAGGAA
CCGACTGGCAGCTCAACGATTGGAGTGCCAGTTTATCGGGTCACTACATCAGTTCATTGGGTGATGGTGACTTTAAATTT
GATAACGAAACCGTCGACAGTTGGCTCACCTTCAGTGCGAGCCTTGGTTATGAGTTAAATGAGCACCATAGCTTTTTGGT
GTCAGTGCGTAACCTAACAGATGAAGAACCGCCCTACGCCTCCTCTCCAACCAATGGCTATGCAAGCTCAGTCCATGATT
GGTTAGGCCGTCGCTGGAACGTACGTTATACCTTCAAGTTTTAA

Upstream 100 bases:

>100_bases
GCGCTTTCTGCAAAACATTAACTAATGCCATGCGTATTGACTCACGCGCAGCTTAGCCACGTCTAAAAAATTCTAATAAG
AGATTTATCGGGAGCTTATC

Downstream 100 bases:

>100_bases
TGTACTGACTTGAGGCATTGGCCGCAGCGTTTAATTGTGGCCAATGCCTTATTCTGACACTTTATAAAGGCACATAGGAT
GAAATTACCTACTGCAAGGG

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 867; Mature: 867

Protein sequence:

>867_residues
MRSSVFLCAVFGLALSPVALAVANELEKTTEQPAADAPVERVAVTGSRLKGVDMEGANPLQSFTQDDITKRGYESVSEFL
RDLPQASSAGTFSETGGVGGADGAPAGASGVSLRGLGSSSTLVLVNGRRVAVDSFSNGSDSFVNVNSIPLSAIERIDVLT
DGASSIYGSDAIAGVINFILRKDFVGHELSAQYGDDTSAHDASRKNLTYAGGFATENSNTTVVVDYATRNAIFNSDRPVN
VTFESSTRMTIDGEDYAESWCGDDTQKSGTRCNYDYVIQRAIQPDFDNIGTTFNHIYRLDNGLEFFAEGMYQKNTGSAYE
APAGYTVDVAGDSANIPQYVHDIDMLDGSVSDVITVRTRFPERRVQEYDTQSYRVLAGLRGELADWSWETAASYGKSTNE
ITNVAGYMNATKLDAAVAADEFNPFNLGRDSSAAQIAALRDPGMRKGESEVLSLDFNISGDLFALPAGTVSSVFGGELRK
EQIFDRPSINAVNNEIIGLGASDAEADRTQYAVYGEFNFPITDTLDVIAALRYDHYSDFGGDVNPKISMRYRPLEDLVLR
ASWSTGFRAPSLSQLGAGTSLSSQYINCGSAEPYNALCGTAGAEFGELEFDQETIGNKDLSAEKSAAINVGVSWNLTEAL
QLTVDYWRYEHDDIVDIDANTTLDACIKDSTKIVTSVAELGDEFGCVVDGQGDISFLRTGYFNVGNQNTDGIDYKLDYRL
DTETAGEFKFYVAGTETLSFERQVTPSTPVEDLLGRLSGASEIARPESVLDLGTDWQLNDWSASLSGHYISSLGDGDFKF
DNETVDSWLTFSASLGYELNEHHSFLVSVRNLTDEEPPYASSPTNGYASSVHDWLGRRWNVRYTFKF

Sequences:

>Translated_867_residues
MRSSVFLCAVFGLALSPVALAVANELEKTTEQPAADAPVERVAVTGSRLKGVDMEGANPLQSFTQDDITKRGYESVSEFL
RDLPQASSAGTFSETGGVGGADGAPAGASGVSLRGLGSSSTLVLVNGRRVAVDSFSNGSDSFVNVNSIPLSAIERIDVLT
DGASSIYGSDAIAGVINFILRKDFVGHELSAQYGDDTSAHDASRKNLTYAGGFATENSNTTVVVDYATRNAIFNSDRPVN
VTFESSTRMTIDGEDYAESWCGDDTQKSGTRCNYDYVIQRAIQPDFDNIGTTFNHIYRLDNGLEFFAEGMYQKNTGSAYE
APAGYTVDVAGDSANIPQYVHDIDMLDGSVSDVITVRTRFPERRVQEYDTQSYRVLAGLRGELADWSWETAASYGKSTNE
ITNVAGYMNATKLDAAVAADEFNPFNLGRDSSAAQIAALRDPGMRKGESEVLSLDFNISGDLFALPAGTVSSVFGGELRK
EQIFDRPSINAVNNEIIGLGASDAEADRTQYAVYGEFNFPITDTLDVIAALRYDHYSDFGGDVNPKISMRYRPLEDLVLR
ASWSTGFRAPSLSQLGAGTSLSSQYINCGSAEPYNALCGTAGAEFGELEFDQETIGNKDLSAEKSAAINVGVSWNLTEAL
QLTVDYWRYEHDDIVDIDANTTLDACIKDSTKIVTSVAELGDEFGCVVDGQGDISFLRTGYFNVGNQNTDGIDYKLDYRL
DTETAGEFKFYVAGTETLSFERQVTPSTPVEDLLGRLSGASEIARPESVLDLGTDWQLNDWSASLSGHYISSLGDGDFKF
DNETVDSWLTFSASLGYELNEHHSFLVSVRNLTDEEPPYASSPTNGYASSVHDWLGRRWNVRYTFKF
>Mature_867_residues
MRSSVFLCAVFGLALSPVALAVANELEKTTEQPAADAPVERVAVTGSRLKGVDMEGANPLQSFTQDDITKRGYESVSEFL
RDLPQASSAGTFSETGGVGGADGAPAGASGVSLRGLGSSSTLVLVNGRRVAVDSFSNGSDSFVNVNSIPLSAIERIDVLT
DGASSIYGSDAIAGVINFILRKDFVGHELSAQYGDDTSAHDASRKNLTYAGGFATENSNTTVVVDYATRNAIFNSDRPVN
VTFESSTRMTIDGEDYAESWCGDDTQKSGTRCNYDYVIQRAIQPDFDNIGTTFNHIYRLDNGLEFFAEGMYQKNTGSAYE
APAGYTVDVAGDSANIPQYVHDIDMLDGSVSDVITVRTRFPERRVQEYDTQSYRVLAGLRGELADWSWETAASYGKSTNE
ITNVAGYMNATKLDAAVAADEFNPFNLGRDSSAAQIAALRDPGMRKGESEVLSLDFNISGDLFALPAGTVSSVFGGELRK
EQIFDRPSINAVNNEIIGLGASDAEADRTQYAVYGEFNFPITDTLDVIAALRYDHYSDFGGDVNPKISMRYRPLEDLVLR
ASWSTGFRAPSLSQLGAGTSLSSQYINCGSAEPYNALCGTAGAEFGELEFDQETIGNKDLSAEKSAAINVGVSWNLTEAL
QLTVDYWRYEHDDIVDIDANTTLDACIKDSTKIVTSVAELGDEFGCVVDGQGDISFLRTGYFNVGNQNTDGIDYKLDYRL
DTETAGEFKFYVAGTETLSFERQVTPSTPVEDLLGRLSGASEIARPESVLDLGTDWQLNDWSASLSGHYISSLGDGDFKF
DNETVDSWLTFSASLGYELNEHHSFLVSVRNLTDEEPPYASSPTNGYASSVHDWLGRRWNVRYTFKF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788478, Length=184, Percent_Identity=31.5217391304348, Blast_Score=83, Evalue=1e-16,
Organism=Escherichia coli, GI1790405, Length=164, Percent_Identity=29.2682926829268, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 94000; Mature: 94000

Theoretical pI: Translated: 4.13; Mature: 4.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSSVFLCAVFGLALSPVALAVANELEKTTEQPAADAPVERVAVTGSRLKGVDMEGANPL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEECCCCCCCCCCCCCCHH
QSFTQDDITKRGYESVSEFLRDLPQASSAGTFSETGGVGGADGAPAGASGVSLRGLGSSS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCCCCCCCEEEECCCCC
TLVLVNGRRVAVDSFSNGSDSFVNVNSIPLSAIERIDVLTDGASSIYGSDAIAGVINFIL
EEEEECCCEEEEECCCCCCCCEEEECCCCHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHH
RKDFVGHELSAQYGDDTSAHDASRKNLTYAGGFATENSNTTVVVDYATRNAIFNSDRPVN
HHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCEEECCCCCEEEEEECCCCCEECCCCCEE
VTFESSTRMTIDGEDYAESWCGDDTQKSGTRCNYDYVIQRAIQPDFDNIGTTFNHIYRLD
EEECCCCEEEECCHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHHCCHHHHHHHHC
NGLEFFAEGMYQKNTGSAYEAPAGYTVDVAGDSANIPQYVHDIDMLDGSVSDVITVRTRF
CCHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCHHHEEEEECCC
PERRVQEYDTQSYRVLAGLRGELADWSWETAASYGKSTNEITNVAGYMNATKLDAAVAAD
CHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHC
EFNPFNLGRDSSAAQIAALRDPGMRKGESEVLSLDFNISGDLFALPAGTVSSVFGGELRK
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCEEEECCCHHHHHHCCHHHH
EQIFDRPSINAVNNEIIGLGASDAEADRTQYAVYGEFNFPITDTLDVIAALRYDHYSDFG
HHHCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCC
GDVNPKISMRYRPLEDLVLRASWSTGFRAPSLSQLGAGTSLSSQYINCGSAEPYNALCGT
CCCCCEEEEEECCHHHHHEEECCCCCCCCCCHHHHCCCCCCCHHHCCCCCCCCCHHHHCC
AGAEFGELEFDQETIGNKDLSAEKSAAINVGVSWNLTEALQLTVDYWRYEHDDIVDIDAN
CCCCCCCCCCCHHHCCCCCCCCCCCCEEEECEECCHHHHHHHHHHHHCCCCCCEEEECCC
TTLDACIKDSTKIVTSVAELGDEFGCVVDGQGDISFLRTGYFNVGNQNTDGIDYKLDYRL
CCHHHHHCCHHHHHHHHHHHCCCCCEEEECCCCEEEEEECEEECCCCCCCCCEEEEEEEE
DTETAGEFKFYVAGTETLSFERQVTPSTPVEDLLGRLSGASEIARPESVLDLGTDWQLND
CCCCCCCEEEEEECCCEEEEECCCCCCCCHHHHHHHHCCCHHHCCCHHHHCCCCCCEECC
WSASLSGHYISSLGDGDFKFDNETVDSWLTFSASLGYELNEHHSFLVSVRNLTDEEPPYA
CCCCCCCCCHHHCCCCCEEECCHHHHHHEEEEHHCCCEECCCCEEEEEEECCCCCCCCCC
SSPTNGYASSVHDWLGRRWNVRYTFKF
CCCCCCHHHHHHHHHCCEEEEEEEEEC
>Mature Secondary Structure
MRSSVFLCAVFGLALSPVALAVANELEKTTEQPAADAPVERVAVTGSRLKGVDMEGANPL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEECCCCCCCCCCCCCCHH
QSFTQDDITKRGYESVSEFLRDLPQASSAGTFSETGGVGGADGAPAGASGVSLRGLGSSS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCCCCCCCEEEECCCCC
TLVLVNGRRVAVDSFSNGSDSFVNVNSIPLSAIERIDVLTDGASSIYGSDAIAGVINFIL
EEEEECCCEEEEECCCCCCCCEEEECCCCHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHH
RKDFVGHELSAQYGDDTSAHDASRKNLTYAGGFATENSNTTVVVDYATRNAIFNSDRPVN
HHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCEEECCCCCEEEEEECCCCCEECCCCCEE
VTFESSTRMTIDGEDYAESWCGDDTQKSGTRCNYDYVIQRAIQPDFDNIGTTFNHIYRLD
EEECCCCEEEECCHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHHCCHHHHHHHHC
NGLEFFAEGMYQKNTGSAYEAPAGYTVDVAGDSANIPQYVHDIDMLDGSVSDVITVRTRF
CCHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCHHHEEEEECCC
PERRVQEYDTQSYRVLAGLRGELADWSWETAASYGKSTNEITNVAGYMNATKLDAAVAAD
CHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHC
EFNPFNLGRDSSAAQIAALRDPGMRKGESEVLSLDFNISGDLFALPAGTVSSVFGGELRK
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCEEEECCCHHHHHHCCHHHH
EQIFDRPSINAVNNEIIGLGASDAEADRTQYAVYGEFNFPITDTLDVIAALRYDHYSDFG
HHHCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCC
GDVNPKISMRYRPLEDLVLRASWSTGFRAPSLSQLGAGTSLSSQYINCGSAEPYNALCGT
CCCCCEEEEEECCHHHHHEEECCCCCCCCCCHHHHCCCCCCCHHHCCCCCCCCCHHHHCC
AGAEFGELEFDQETIGNKDLSAEKSAAINVGVSWNLTEALQLTVDYWRYEHDDIVDIDAN
CCCCCCCCCCCHHHCCCCCCCCCCCCEEEECEECCHHHHHHHHHHHHCCCCCCEEEECCC
TTLDACIKDSTKIVTSVAELGDEFGCVVDGQGDISFLRTGYFNVGNQNTDGIDYKLDYRL
CCHHHHHCCHHHHHHHHHHHCCCCCEEEECCCCEEEEEECEEECCCCCCCCCEEEEEEEE
DTETAGEFKFYVAGTETLSFERQVTPSTPVEDLLGRLSGASEIARPESVLDLGTDWQLND
CCCCCCCEEEEEECCCEEEEECCCCCCCCHHHHHHHHCCCHHHCCCHHHHCCCCCCEECC
WSASLSGHYISSLGDGDFKFDNETVDSWLTFSASLGYELNEHHSFLVSVRNLTDEEPPYA
CCCCCCCCCHHHCCCCCEEECCHHHHHHEEEEHHCCCEECCCCEEEEEEECCCCCCCCCC
SSPTNGYASSVHDWLGRRWNVRYTFKF
CCCCCCHHHHHHHHHCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11598060 [H]