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The map label for this gene is fumB [H]

Identifier: 126174144

GI number: 126174144

Start: 2228020

End: 2229564

Strand: Direct

Name: fumB [H]

Synonym: Sbal_1918

Alternate gene names: 126174144

Gene position: 2228020-2229564 (Clockwise)

Preceding gene: 126174141

Following gene: 126174145

Centisome position: 43.45

GC content: 49.84

Gene sequence:

>1545_bases
ATGTCATCTAACCATGAAATGTCTGTCAATTCTGTGATTAAGCAAGCCGACTTTATTGAAAGTGTGGCGGATGCTTTGCA
ATACATCTCTTATTACCACCCGAAGGATTTTGTTGATGCCATGAGCCAAGCTTATGAGCGTGAACAAAGTGCTGCGGCGA
AGGATGCCATTGCCCAGATATTAATTAACTCGCGTATGTCGGCCGAAGGCAAGCGTCCTCTGTGTCAAGACACTGGGATT
GTCACCACCTTCGTTAAAGTGGGTATGGGTGTCCAATGGGACAAAACCGATATGACAATTCAGGAAATGGTCGACGAAGG
TGTTCGTCGTGCTTATACCAATCCTGATAACCCACTGCGTGCATCAATAGTGTCAGATCCTGCGGGCAGCCGTAAAAATA
CCAAAGATAACACGCCATCTGTGGTGCATATCGACATGGTGCCAGGCAACCACATCGAAGTAGCGATTGCGGCCAAAGGC
GGCGGCAGTGAAAACAAAGCTAAGATGGTGATGTTAAACCCATCTGATGATATCGCCGCTTGGGTAGAAAAAACGCTACC
GACCATGGGCGCGGGCTGGTGTCCACCGGGTATGCTGGGTATAGGCATCGGCGGCACGGCAGAAAAAGCGGCCGTATTAG
CCAAAGAAGCGCTGATGGAAAGCGTGGATATCCATGAGTTGATGGCCCGTGGCGCGCAAACGACCGAAGAAAAGCTGCGT
TTAGACATTTTTGAGCGCGCGAATAGCTTAGGCATAGGTGCACAAGGTCTTGGCGGTTTAACCACAGTGTTAGACGTTAA
AATCAAATCGGCACCAACCCACGCAGCTTCAAAGCCTGTGGTGATGATCCCCAACTGTGCAGCGACTCGCCACGTACATT
TCCATTTAGACGGCTCAGGTCCAGCTGAATTGACGCCACCATCATTGTCAGATTGGCCAGAAATCACCCGTGAAGTGGGT
GATGATGTGCGCCGTGTTAACTTAGATACTGTGACTCAAGCGGATATCGAGCAGTGGCAGAGCGGTGAAACCATTCTGCT
GAGCGGTAAAATGCTCACAGGTCGCGATGCTGCCCATAAGCGCATTCAAAGCCTAATCGAAAGTGGTGAAGGTCTGCCAG
AAGGCGTGGACTTTACTGGTAAATTTATTTACTACGTTGGCCCCGTTGATCCAGTGGGCACTGAAGTTGTCGGTCCTGCG
GGCCCAACCACAGCAACCCGTATGGATAAATTCACTGACTTAATGTTAGAGAAAACTGGCTTGATGGGCATGATAGGTAA
AGCTGAGCGCGGCCCTGCGACAGTTGAATCAATCAAAAAACACAAAGCGGTATATCTGATGGCCGTTGGCGGCGCAGCGT
ATCTGGTGTCTAAAGCGATTAAACATTCTCGCGTCGTGGCCTTTGCCGATTTAGGCATGGAAGCGATTTATGAGTTTGAC
GTGCAAGATATGCCAGTGACTGTGGCTGTGGATTCAAACGGCGTGAACGCCCATGAAACTGGCCCAGCAATTTGGAAAGT
GAATATCGCCAACGCGAAAGCCTAA

Upstream 100 bases:

>100_bases
TGTGATGTAGCCCAAAAAGATGGGAAAGAGTATCATATTGGCCCAGAAAGAATTAAACAAAAATAAGTTAGGCCGCAAAG
GCTGCTGGAGCGCTCCTACT

Downstream 100 bases:

>100_bases
GTGGGAGTGAAGCTAAGGCATTAGCGGATTGAACTTGATGGCTTTTTAAGCAAAGCAGTTTAAGTTTGCGCTCTGATATC
GGTTGCTTCGGTAATCTGAT

Product: tartrate/fumarate subfamily Fe-S type hydro-lyase subunit alpha

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MSSNHEMSVNSVIKQADFIESVADALQYISYYHPKDFVDAMSQAYEREQSAAAKDAIAQILINSRMSAEGKRPLCQDTGI
VTTFVKVGMGVQWDKTDMTIQEMVDEGVRRAYTNPDNPLRASIVSDPAGSRKNTKDNTPSVVHIDMVPGNHIEVAIAAKG
GGSENKAKMVMLNPSDDIAAWVEKTLPTMGAGWCPPGMLGIGIGGTAEKAAVLAKEALMESVDIHELMARGAQTTEEKLR
LDIFERANSLGIGAQGLGGLTTVLDVKIKSAPTHAASKPVVMIPNCAATRHVHFHLDGSGPAELTPPSLSDWPEITREVG
DDVRRVNLDTVTQADIEQWQSGETILLSGKMLTGRDAAHKRIQSLIESGEGLPEGVDFTGKFIYYVGPVDPVGTEVVGPA
GPTTATRMDKFTDLMLEKTGLMGMIGKAERGPATVESIKKHKAVYLMAVGGAAYLVSKAIKHSRVVAFADLGMEAIYEFD
VQDMPVTVAVDSNGVNAHETGPAIWKVNIANAKA

Sequences:

>Translated_514_residues
MSSNHEMSVNSVIKQADFIESVADALQYISYYHPKDFVDAMSQAYEREQSAAAKDAIAQILINSRMSAEGKRPLCQDTGI
VTTFVKVGMGVQWDKTDMTIQEMVDEGVRRAYTNPDNPLRASIVSDPAGSRKNTKDNTPSVVHIDMVPGNHIEVAIAAKG
GGSENKAKMVMLNPSDDIAAWVEKTLPTMGAGWCPPGMLGIGIGGTAEKAAVLAKEALMESVDIHELMARGAQTTEEKLR
LDIFERANSLGIGAQGLGGLTTVLDVKIKSAPTHAASKPVVMIPNCAATRHVHFHLDGSGPAELTPPSLSDWPEITREVG
DDVRRVNLDTVTQADIEQWQSGETILLSGKMLTGRDAAHKRIQSLIESGEGLPEGVDFTGKFIYYVGPVDPVGTEVVGPA
GPTTATRMDKFTDLMLEKTGLMGMIGKAERGPATVESIKKHKAVYLMAVGGAAYLVSKAIKHSRVVAFADLGMEAIYEFD
VQDMPVTVAVDSNGVNAHETGPAIWKVNIANAKA
>Mature_513_residues
SSNHEMSVNSVIKQADFIESVADALQYISYYHPKDFVDAMSQAYEREQSAAAKDAIAQILINSRMSAEGKRPLCQDTGIV
TTFVKVGMGVQWDKTDMTIQEMVDEGVRRAYTNPDNPLRASIVSDPAGSRKNTKDNTPSVVHIDMVPGNHIEVAIAAKGG
GSENKAKMVMLNPSDDIAAWVEKTLPTMGAGWCPPGMLGIGIGGTAEKAAVLAKEALMESVDIHELMARGAQTTEEKLRL
DIFERANSLGIGAQGLGGLTTVLDVKIKSAPTHAASKPVVMIPNCAATRHVHFHLDGSGPAELTPPSLSDWPEITREVGD
DVRRVNLDTVTQADIEQWQSGETILLSGKMLTGRDAAHKRIQSLIESGEGLPEGVDFTGKFIYYVGPVDPVGTEVVGPAG
PTTATRMDKFTDLMLEKTGLMGMIGKAERGPATVESIKKHKAVYLMAVGGAAYLVSKAIKHSRVVAFADLGMEAIYEFDV
QDMPVTVAVDSNGVNAHETGPAIWKVNIANAKA

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=474, Percent_Identity=32.0675105485232, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1787897, Length=468, Percent_Identity=33.1196581196581, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1789442, Length=295, Percent_Identity=29.8305084745763, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1789443, Length=179, Percent_Identity=30.7262569832402, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 55007; Mature: 54876

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNHEMSVNSVIKQADFIESVADALQYISYYHPKDFVDAMSQAYEREQSAAAKDAIAQI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LINSRMSAEGKRPLCQDTGIVTTFVKVGMGVQWDKTDMTIQEMVDEGVRRAYTNPDNPLR
HHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHCCCCCCCEE
ASIVSDPAGSRKNTKDNTPSVVHIDMVPGNHIEVAIAAKGGGSENKAKMVMLNPSDDIAA
EEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCEEEEECCCHHHHH
WVEKTLPTMGAGWCPPGMLGIGIGGTAEKAAVLAKEALMESVDIHELMARGAQTTEEKLR
HHHHHCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHH
LDIFERANSLGIGAQGLGGLTTVLDVKIKSAPTHAASKPVVMIPNCAATRHVHFHLDGSG
HHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCEEEEEEEECCCC
PAELTPPSLSDWPEITREVGDDVRRVNLDTVTQADIEQWQSGETILLSGKMLTGRDAAHK
CCCCCCCCCCHHHHHHHHHCCHHHEECCHHHHHHHHHHHCCCCEEEEECCEECCCHHHHH
RIQSLIESGEGLPEGVDFTGKFIYYVGPVDPVGTEVVGPAGPTTATRMDKFTDLMLEKTG
HHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
LMGMIGKAERGPATVESIKKHKAVYLMAVGGAAYLVSKAIKHSRVVAFADLGMEAIYEFD
CHHCCCCCCCCCHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHEEEEHHCCHHHHHCCC
VQDMPVTVAVDSNGVNAHETGPAIWKVNIANAKA
CCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCC
>Mature Secondary Structure 
SSNHEMSVNSVIKQADFIESVADALQYISYYHPKDFVDAMSQAYEREQSAAAKDAIAQI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LINSRMSAEGKRPLCQDTGIVTTFVKVGMGVQWDKTDMTIQEMVDEGVRRAYTNPDNPLR
HHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHCCCCCCCEE
ASIVSDPAGSRKNTKDNTPSVVHIDMVPGNHIEVAIAAKGGGSENKAKMVMLNPSDDIAA
EEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCEEEEECCCHHHHH
WVEKTLPTMGAGWCPPGMLGIGIGGTAEKAAVLAKEALMESVDIHELMARGAQTTEEKLR
HHHHHCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHH
LDIFERANSLGIGAQGLGGLTTVLDVKIKSAPTHAASKPVVMIPNCAATRHVHFHLDGSG
HHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCEEEEEEEECCCC
PAELTPPSLSDWPEITREVGDDVRRVNLDTVTQADIEQWQSGETILLSGKMLTGRDAAHK
CCCCCCCCCCHHHHHHHHHCCHHHEECCHHHHHHHHHHHCCCCEEEEECCEECCCHHHHH
RIQSLIESGEGLPEGVDFTGKFIYYVGPVDPVGTEVVGPAGPTTATRMDKFTDLMLEKTG
HHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
LMGMIGKAERGPATVESIKKHKAVYLMAVGGAAYLVSKAIKHSRVVAFADLGMEAIYEFD
CHHCCCCCCCCCHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHEEEEHHCCHHHHHCCC
VQDMPVTVAVDSNGVNAHETGPAIWKVNIANAKA
CCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]