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The map label for this gene is iaaA [H]

Identifier: 126174130

GI number: 126174130

Start: 2210839

End: 2211870

Strand: Direct

Name: iaaA [H]

Synonym: Sbal_1904

Alternate gene names: 126174130

Gene position: 2210839-2211870 (Clockwise)

Preceding gene: 126174127

Following gene: 126174131

Centisome position: 43.12

GC content: 47.67

Gene sequence:

>1032_bases
ATGAAAACAAAATTAGCCACCTTTAGTTTAATTATCTCGGGTTTACTTTTTATGTCACACAACACCTTTGCCAATGACAA
ACCGTTCGCGATTGCGATCCACGGCGGCGCTGGCACGATTTCTAAGGCTAATCTCACCCCTGAACAACGCCAAGCCTACA
AAGATAAGTTAAAGGAAGCCGTTGATAAAGGCTCAAAAGTGTTAGAAGAGGGCGGTGACAGTTTAGTTGCGGTGCAAACA
GCTATTAACATTCTCGAAAATAGCCCACTGTTCAATGCTGGTATAGGCGCGGTGTACACCTACGACGGTGGGCATGAGTT
AGATGCGTCTATTATGGATGGCAAAACCATGAATGCTGGCGCAATTGCTGGAGTGCGCCATATCGCCAATCCCATTGATC
TCGCTTTAGCTGTGATGAATAAGTCTGAACATGTGATGCTCTCGGGCGCAGGTGCAGAAGAATTTGCACTGACTCAAGGC
TTTAGCTTAGTGCCAAACAGTCATTTTGATACTGACGAGCGTTATCAACAATTACTCGATGCTAGGCAAAAACTACAAGC
TGCGGCTAAATCAGAGCAAGTTGCTAATGTTGAGATGAAGGACCTCGATTACAAATTTGGTACCGTTGGTGCTGTCGCCT
TAGATAAAAAGGGTAACTTAGCTGCAGGTACATCGACCGGTGGTATGACGGCAAAACGCTTTGGTCGTATTGGTGACTCT
CCTGTCATCGGTGCTGGCACTTATGCTGAAAACGGGGTGTGCGCCGTATCGGCAACCGGACATGGTGAATTCTTTATTCG
TTACCAAGTGGCTGGGGACATTTGTGCCAAGGTGAAATACCAGCAGAAATCCATTATTCAAGCCGCCGATGAAGTGATCA
ATCAACGCCTTATCACCGCAGGTGGTTCGGGCGGCGTGATTGCCGTCGATCAGCGCGGTAATATCGCCACGCCATTTAAC
ACTGAAGGCATGTACCGCGCTACCCGTAGCAATGGTGAACCCGCCCAAGTGCTGATTTGGCAAGACCAATAA

Upstream 100 bases:

>100_bases
GCATCATTGTCGCCAGAATAAAACCAACAGGCAAGGAGATGATTGAAATTACACGATGAAATGGTTACAAAGGACGCTAC
TTTTTAAGGGGATGTAAAGA

Downstream 100 bases:

>100_bases
CTTGAATTGTAAAAGCAGTTGGTTCGAGACGGACATTGCTTACAAGATTGTTTAGAAGATTGCTTAGAAACATCACTTAT
AAACCTTAAAAAGCGAGTGC

Product: peptidase T2 asparaginase 2

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MKTKLATFSLIISGLLFMSHNTFANDKPFAIAIHGGAGTISKANLTPEQRQAYKDKLKEAVDKGSKVLEEGGDSLVAVQT
AINILENSPLFNAGIGAVYTYDGGHELDASIMDGKTMNAGAIAGVRHIANPIDLALAVMNKSEHVMLSGAGAEEFALTQG
FSLVPNSHFDTDERYQQLLDARQKLQAAAKSEQVANVEMKDLDYKFGTVGAVALDKKGNLAAGTSTGGMTAKRFGRIGDS
PVIGAGTYAENGVCAVSATGHGEFFIRYQVAGDICAKVKYQQKSIIQAADEVINQRLITAGGSGGVIAVDQRGNIATPFN
TEGMYRATRSNGEPAQVLIWQDQ

Sequences:

>Translated_343_residues
MKTKLATFSLIISGLLFMSHNTFANDKPFAIAIHGGAGTISKANLTPEQRQAYKDKLKEAVDKGSKVLEEGGDSLVAVQT
AINILENSPLFNAGIGAVYTYDGGHELDASIMDGKTMNAGAIAGVRHIANPIDLALAVMNKSEHVMLSGAGAEEFALTQG
FSLVPNSHFDTDERYQQLLDARQKLQAAAKSEQVANVEMKDLDYKFGTVGAVALDKKGNLAAGTSTGGMTAKRFGRIGDS
PVIGAGTYAENGVCAVSATGHGEFFIRYQVAGDICAKVKYQQKSIIQAADEVINQRLITAGGSGGVIAVDQRGNIATPFN
TEGMYRATRSNGEPAQVLIWQDQ
>Mature_343_residues
MKTKLATFSLIISGLLFMSHNTFANDKPFAIAIHGGAGTISKANLTPEQRQAYKDKLKEAVDKGSKVLEEGGDSLVAVQT
AINILENSPLFNAGIGAVYTYDGGHELDASIMDGKTMNAGAIAGVRHIANPIDLALAVMNKSEHVMLSGAGAEEFALTQG
FSLVPNSHFDTDERYQQLLDARQKLQAAAKSEQVANVEMKDLDYKFGTVGAVALDKKGNLAAGTSTGGMTAKRFGRIGDS
PVIGAGTYAENGVCAVSATGHGEFFIRYQVAGDICAKVKYQQKSIIQAADEVINQRLITAGGSGGVIAVDQRGNIATPFN
TEGMYRATRSNGEPAQVLIWQDQ

Specific function: Degrades proteins damaged by L-isoaspartyl residue formation (also known as beta-Asp residues). Degrades L- isoaspartyl-containing di- and tripeptides. Acts best on iso-Asp- Leu, followed by iso-Asp-Ala, -His and to a lesser extent iso-Asp- Lys, -Phe and

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=306, Percent_Identity=38.8888888888889, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI145275200, Length=306, Percent_Identity=38.8888888888889, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI98991769, Length=323, Percent_Identity=29.4117647058824, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI285002251, Length=231, Percent_Identity=31.1688311688312, Blast_Score=92, Evalue=6e-19,
Organism=Escherichia coli, GI1787050, Length=313, Percent_Identity=50.7987220447284, Blast_Score=292, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI32564649, Length=301, Percent_Identity=26.2458471760797, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI18921183, Length=305, Percent_Identity=37.0491803278689, Blast_Score=167, Evalue=8e-42,
Organism=Drosophila melanogaster, GI21357893, Length=270, Percent_Identity=32.5925925925926, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI221330102, Length=232, Percent_Identity=33.1896551724138, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24652135, Length=232, Percent_Identity=33.1896551724138, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 36290; Mature: 36290

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKLATFSLIISGLLFMSHNTFANDKPFAIAIHGGAGTISKANLTPEQRQAYKDKLKEA
CCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEECCCCHHHHHHHHHHHHHH
VDKGSKVLEEGGDSLVAVQTAINILENSPLFNAGIGAVYTYDGGHELDASIMDGKTMNAG
HHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCCCCEEEEECCCCEECCEEECCCCCCCH
AIAGVRHIANPIDLALAVMNKSEHVMLSGAGAEEFALTQGFSLVPNSHFDTDERYQQLLD
HHHHHHHHCCHHHHHHHEECCCCEEEEECCCCHHHHHHCCCEECCCCCCCHHHHHHHHHH
ARQKLQAAAKSEQVANVEMKDLDYKFGTVGAVALDKKGNLAAGTSTGGMTAKRFGRIGDS
HHHHHHHHHHHHHHCCCCHHHCCEECCCEEEEEECCCCCEECCCCCCCCCHHHHCCCCCC
PVIGAGTYAENGVCAVSATGHGEFFIRYQVAGDICAKVKYQQKSIIQAADEVINQRLITA
CEEECCCCCCCCEEEEEECCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHEEEEE
GGSGGVIAVDQRGNIATPFNTEGMYRATRSNGEPAQVLIWQDQ
CCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCCEEEEEEECC
>Mature Secondary Structure
MKTKLATFSLIISGLLFMSHNTFANDKPFAIAIHGGAGTISKANLTPEQRQAYKDKLKEA
CCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEECCCCHHHHHHHHHHHHHH
VDKGSKVLEEGGDSLVAVQTAINILENSPLFNAGIGAVYTYDGGHELDASIMDGKTMNAG
HHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCCCCEEEEECCCCEECCEEECCCCCCCH
AIAGVRHIANPIDLALAVMNKSEHVMLSGAGAEEFALTQGFSLVPNSHFDTDERYQQLLD
HHHHHHHHCCHHHHHHHEECCCCEEEEECCCCHHHHHHCCCEECCCCCCCHHHHHHHHHH
ARQKLQAAAKSEQVANVEMKDLDYKFGTVGAVALDKKGNLAAGTSTGGMTAKRFGRIGDS
HHHHHHHHHHHHHHCCCCHHHCCEECCCEEEEEECCCCCEECCCCCCCCCHHHHCCCCCC
PVIGAGTYAENGVCAVSATGHGEFFIRYQVAGDICAKVKYQQKSIIQAADEVINQRLITA
CEEECCCCCCCCEEEEEECCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHEEEEE
GGSGGVIAVDQRGNIATPFNTEGMYRATRSNGEPAQVLIWQDQ
CCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]