Click here to switch to the map view.

The map label for this gene is nadB [H]

Identifier: 126173432

GI number: 126173432

Start: 1375895

End: 1377508

Strand: Reverse

Name: nadB [H]

Synonym: Sbal_1194

Alternate gene names: 126173432

Gene position: 1377508-1375895 (Counterclockwise)

Preceding gene: 126173445

Following gene: 126173421

Centisome position: 26.87

GC content: 49.57

Gene sequence:

>1614_bases
ATGAAACAAGTAGTTGAACACCAATCTGACATATTAGTCATAGGTAGCGGTGCCGCAGGTCTGACACTAGCTTTGCATCT
TGCTGAAAAAGCAAAAGTAATTCTGCTTTCAAAAGGCCCACTCTCGGAAGGGTCGACTTACTACGCCCAGGGCGGTATCG
CCTCCGTCTTCGACGAAAGTGACACTATCGAATCCCATGTTGCAGACACATTAATCGCAGGTGCGGGTTTATGCGACAAA
GACGTCGTCACTTTTACCGCTGAAAATGCTAAGAGTGCGATGCAATGGCTTATCGAATGTGGCGTCGCCTTCGATAAAGA
AGAAACCACTGACGGTAATGCAAAAGACGCACCTTACCATTTAACCCGTGAAGGCGGCCATAGCCACAGACGTATTCTGC
ACGCTGCCGATGCCACAGGCAAAGAAGTGCAAACGACACTGCAAGAACGCGCACTCGCCCACCCCAATATTCAAGTTTTA
GAACGCTATAACGCCATCGACCTCATCACCACCCGTAAGTTAAATCGCCCAGGTAATCGTGTGTTAGGCGCTTATGTGTG
GAATCGCAACGAAGAACACGTCGAAACCGTTAAGGCCAAGTTTGTTGCGTTAGCAACAGGCGGTAGCTCTAAGGTTTATC
AATATACTTCAAACCCCGATGTCGCTAGCGGCGATGGTATTGCCATGGCATGGCGTGCAGGTTGCCGCGTGGCGAATATG
GAGTTCAACCAATTCCATCCGACATGTTTGTACCATGCCGATGCGCGCAACTTCTTACTCACCGAAGCCCTGCGTGGTGA
AGGTGCTTATCTGCGTCGTCCAGACGGCAGCCGTTTTATGCCGGACTTTGATGAACGCGCTGAACTCGCACCCCGTGATA
TCGTTGCCCGCGCCATCGATTATGAGATGAAACGACTCGGTGCCGATTGCGTGTATCTGGATATCAGCCATAAAGATGCT
GACTTTGTGATGAAGCATTTCCCAACCATCTATAGCCGTTGCTTAGAGCTGGGAATTGATATCACTAAAGATGCAATCCC
TGTAGTGCCTGCCGCGCACTATACCTGCGGCGGCGTGATGACAGATATGCACGGTCAAACCGATCTCAATGGTCTCTATG
CCATTGGTGAAGTGGCCTATACGGGCTTGCATGGCGCGAATCGTCTGGCGAGTAACTCGCTACTCGAATGTCTAGTGTTT
GCCCGTGCGGCGTCACATGATATAGAGAGCCAGCTGTATAAGATTCAAATGCCGGGACAAATTCCCGCATGGGATGAAAG
TAAAGTCTCTAACTCGGACGAAGAAGTGGTCATCGCCCATAACTGGCACGAACTGCGGTTATTCATGTGGGACTACGTTG
GGATTGTGCGCTCGGATAAACGTCTGGAGCGTGCTCTGCGTCGCTGCCTGATGTTACAGCAAGAAATTCAGGAATATTAC
AGTAACTTCAGAGTCAGCAATAACTTGCTCGAATTACGTAACTTAGTGCAAGTGGCCGAGTTGATTATTCGCTGTGCCAT
GGAGCGTAAAGAAAGCCGTGGTCTGCATTTTAATATCGATCACCCAGAGAAATTAGACGCGCCGCGCCCGACTATATTAC
TACCAGAAAAGTAA

Upstream 100 bases:

>100_bases
CTAAAAAATATACAAAAAAAGTAAGGTTAAAAGGAAAGTACCCATAATCCGTGAGTTGGTTTACCATAGTTGGCACTCTT
CAGTCACATCATGATACCCG

Downstream 100 bases:

>100_bases
CCGATTAAGTTCTTATGTGTTTATAGGCTTAGGTTCTTATTTGGCTTGCTGAAAACAGACTAAATCTCAGTATGAGGTTT
AGTCTGTTATATTTTAGCCC

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MKQVVEHQSDILVIGSGAAGLTLALHLAEKAKVILLSKGPLSEGSTYYAQGGIASVFDESDTIESHVADTLIAGAGLCDK
DVVTFTAENAKSAMQWLIECGVAFDKEETTDGNAKDAPYHLTREGGHSHRRILHAADATGKEVQTTLQERALAHPNIQVL
ERYNAIDLITTRKLNRPGNRVLGAYVWNRNEEHVETVKAKFVALATGGSSKVYQYTSNPDVASGDGIAMAWRAGCRVANM
EFNQFHPTCLYHADARNFLLTEALRGEGAYLRRPDGSRFMPDFDERAELAPRDIVARAIDYEMKRLGADCVYLDISHKDA
DFVMKHFPTIYSRCLELGIDITKDAIPVVPAAHYTCGGVMTDMHGQTDLNGLYAIGEVAYTGLHGANRLASNSLLECLVF
ARAASHDIESQLYKIQMPGQIPAWDESKVSNSDEEVVIAHNWHELRLFMWDYVGIVRSDKRLERALRRCLMLQQEIQEYY
SNFRVSNNLLELRNLVQVAELIIRCAMERKESRGLHFNIDHPEKLDAPRPTILLPEK

Sequences:

>Translated_537_residues
MKQVVEHQSDILVIGSGAAGLTLALHLAEKAKVILLSKGPLSEGSTYYAQGGIASVFDESDTIESHVADTLIAGAGLCDK
DVVTFTAENAKSAMQWLIECGVAFDKEETTDGNAKDAPYHLTREGGHSHRRILHAADATGKEVQTTLQERALAHPNIQVL
ERYNAIDLITTRKLNRPGNRVLGAYVWNRNEEHVETVKAKFVALATGGSSKVYQYTSNPDVASGDGIAMAWRAGCRVANM
EFNQFHPTCLYHADARNFLLTEALRGEGAYLRRPDGSRFMPDFDERAELAPRDIVARAIDYEMKRLGADCVYLDISHKDA
DFVMKHFPTIYSRCLELGIDITKDAIPVVPAAHYTCGGVMTDMHGQTDLNGLYAIGEVAYTGLHGANRLASNSLLECLVF
ARAASHDIESQLYKIQMPGQIPAWDESKVSNSDEEVVIAHNWHELRLFMWDYVGIVRSDKRLERALRRCLMLQQEIQEYY
SNFRVSNNLLELRNLVQVAELIIRCAMERKESRGLHFNIDHPEKLDAPRPTILLPEK
>Mature_537_residues
MKQVVEHQSDILVIGSGAAGLTLALHLAEKAKVILLSKGPLSEGSTYYAQGGIASVFDESDTIESHVADTLIAGAGLCDK
DVVTFTAENAKSAMQWLIECGVAFDKEETTDGNAKDAPYHLTREGGHSHRRILHAADATGKEVQTTLQERALAHPNIQVL
ERYNAIDLITTRKLNRPGNRVLGAYVWNRNEEHVETVKAKFVALATGGSSKVYQYTSNPDVASGDGIAMAWRAGCRVANM
EFNQFHPTCLYHADARNFLLTEALRGEGAYLRRPDGSRFMPDFDERAELAPRDIVARAIDYEMKRLGADCVYLDISHKDA
DFVMKHFPTIYSRCLELGIDITKDAIPVVPAAHYTCGGVMTDMHGQTDLNGLYAIGEVAYTGLHGANRLASNSLLECLVF
ARAASHDIESQLYKIQMPGQIPAWDESKVSNSDEEVVIAHNWHELRLFMWDYVGIVRSDKRLERALRRCLMLQQEIQEYY
SNFRVSNNLLELRNLVQVAELIIRCAMERKESRGLHFNIDHPEKLDAPRPTILLPEK

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=517, Percent_Identity=32.1083172147002, Blast_Score=193, Evalue=3e-49,
Organism=Escherichia coli, GI1788928, Length=536, Percent_Identity=71.455223880597, Blast_Score=809, Evalue=0.0,
Organism=Escherichia coli, GI1790597, Length=528, Percent_Identity=30.8712121212121, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1786942, Length=564, Percent_Identity=31.0283687943262, Blast_Score=186, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17550100, Length=571, Percent_Identity=31.8739054290718, Blast_Score=198, Evalue=6e-51,
Organism=Caenorhabditis elegans, GI17505833, Length=561, Percent_Identity=30.4812834224599, Blast_Score=196, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI71986328, Length=471, Percent_Identity=27.6008492569002, Blast_Score=102, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6322701, Length=562, Percent_Identity=30.7829181494662, Blast_Score=206, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6322416, Length=570, Percent_Identity=30, Blast_Score=198, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6322511, Length=487, Percent_Identity=26.2833675564682, Blast_Score=97, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24663005, Length=565, Percent_Identity=30.4424778761062, Blast_Score=201, Evalue=1e-51,
Organism=Drosophila melanogaster, GI17137288, Length=555, Percent_Identity=31.1711711711712, Blast_Score=197, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24655642, Length=555, Percent_Identity=31.1711711711712, Blast_Score=197, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24655647, Length=555, Percent_Identity=31.1711711711712, Blast_Score=197, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 59895; Mature: 59895

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQVVEHQSDILVIGSGAAGLTLALHLAEKAKVILLSKGPLSEGSTYYAQGGIASVFDES
CCCHHCCCCCEEEEECCCCCHHHEEEECCCCEEEEEECCCCCCCCEEEECCCCHHHCCCC
DTIESHVADTLIAGAGLCDKDVVTFTAENAKSAMQWLIECGVAFDKEETTDGNAKDAPYH
HHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEE
LTREGGHSHRRILHAADATGKEVQTTLQERALAHPNIQVLERYNAIDLITTRKLNRPGNR
EECCCCCHHHHEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHCCCEEEEEECCCCCCCCE
VLGAYVWNRNEEHVETVKAKFVALATGGSSKVYQYTSNPDVASGDGIAMAWRAGCRVANM
EEEEEEECCCHHHHHHHHHEEEEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCEEECC
EFNQFHPTCLYHADARNFLLTEALRGEGAYLRRPDGSRFMPDFDERAELAPRDIVARAID
CCCCCCCEEEEECCCCHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHCCHHHHHHHHHH
YEMKRLGADCVYLDISHKDADFVMKHFPTIYSRCLELGIDITKDAIPVVPAAHYTCGGVM
HHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEE
TDMHGQTDLNGLYAIGEVAYTGLHGANRLASNSLLECLVFARAASHDIESQLYKIQMPGQ
ECCCCCCCCCHHEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCEEEEECCCC
IPAWDESKVSNSDEEVVIAHNWHELRLFMWDYVGIVRSDKRLERALRRCLMLQQEIQEYY
CCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SNFRVSNNLLELRNLVQVAELIIRCAMERKESRGLHFNIDHPEKLDAPRPTILLPEK
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHCCCCCCEEECCCC
>Mature Secondary Structure
MKQVVEHQSDILVIGSGAAGLTLALHLAEKAKVILLSKGPLSEGSTYYAQGGIASVFDES
CCCHHCCCCCEEEEECCCCCHHHEEEECCCCEEEEEECCCCCCCCEEEECCCCHHHCCCC
DTIESHVADTLIAGAGLCDKDVVTFTAENAKSAMQWLIECGVAFDKEETTDGNAKDAPYH
HHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEE
LTREGGHSHRRILHAADATGKEVQTTLQERALAHPNIQVLERYNAIDLITTRKLNRPGNR
EECCCCCHHHHEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHCCCEEEEEECCCCCCCCE
VLGAYVWNRNEEHVETVKAKFVALATGGSSKVYQYTSNPDVASGDGIAMAWRAGCRVANM
EEEEEEECCCHHHHHHHHHEEEEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCEEECC
EFNQFHPTCLYHADARNFLLTEALRGEGAYLRRPDGSRFMPDFDERAELAPRDIVARAID
CCCCCCCEEEEECCCCHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHCCHHHHHHHHHH
YEMKRLGADCVYLDISHKDADFVMKHFPTIYSRCLELGIDITKDAIPVVPAAHYTCGGVM
HHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEE
TDMHGQTDLNGLYAIGEVAYTGLHGANRLASNSLLECLVFARAASHDIESQLYKIQMPGQ
ECCCCCCCCCHHEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCEEEEECCCC
IPAWDESKVSNSDEEVVIAHNWHELRLFMWDYVGIVRSDKRLERALRRCLMLQQEIQEYY
CCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SNFRVSNNLLELRNLVQVAELIIRCAMERKESRGLHFNIDHPEKLDAPRPTILLPEK
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHCCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]