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The map label for this gene is rumB [H]

Identifier: 126173113

GI number: 126173113

Start: 979567

End: 980739

Strand: Direct

Name: rumB [H]

Synonym: Sbal_0868

Alternate gene names: 126173113

Gene position: 979567-980739 (Clockwise)

Preceding gene: 126173112

Following gene: 126173114

Centisome position: 19.1

GC content: 48.34

Gene sequence:

>1173_bases
GTGAGTGCTGCTATTGTGAATGCCGTTAAGCAATGTGGTTATTTTAATCAAGGTCAGTGCCTTTCTTGCCGGCATATTCA
GCAGCCTCTAGCGCAGCAAGTAGCGGTTAAAACCCAAACGTTACAGCAATTACTCGCGCCTTTTATTCCCGCAAATTCGG
CAGAACTTTTTCTGCCACCCATTACTGGCGATGACAGCGGCTTTCGTAATAAAGCCAAAATGGTGGTGCTCGGTGCCGCC
CATGAACCCGTGTTGGGGATTGTGGGCCCAAGTGGCGAGGCGGTGGATCTTTGTGACTGCCTGTTATATCCCGCTGATAT
GCAAGCCTTGTTGCACCGTTTAACCCGCTTTGTGCAGCAGGCGGGGCTTCCTCCCTATCGGGTTGATAAAGCCAAGGGCG
AGCTTAAGTTTATTCTGCTGACACGCAGCCAAGTGCGCGGCGAATATCTGCTGCGTTTTGTGCTGCGATCACACAATGGC
ATTGAACGTATCGAACGCGAATTACCTGCATTACTGGCCGAATATCCGCAGATAAAAGTAGTGTCAGTCAATATTCAACC
GATTCATATGGCGATACTTGAGGGCGATGAAGAGATTTTTTTAACTGAGAATACCCGTCTCGAAGAACGTTTTAATCATG
TGCCTTTGTTCATTCGGCCTAAGAGTTTTTTCCAGACCAATCCGCAGGTTGCGGCTCAGTTATATCAAACCGCCCGTGAG
TGGGTTGCCGAGTTTTCTCCTCGCTCGTTATGGGATCTGTTTTGTGGCGTCGGCGGTTTCGGACTGCACTGCGCATCCAA
TGACATTACACTCACAGGGATTGAAATTGAAGCCGAGGCGATTGCCTGCGCGCAAATGTCAGCGCAAATGATGGGGCTCG
AAAACGTGCAATTTATGGCGCTCGATTCCACCGACTTTGCCAAGGGCAAAAGCGCTGCTGATAAGCCGGATTTGATCATT
GTTAATCCACCAAGGCGCGGCATTGGCGAGGCTTTATGCCAGTCATTAAGCGAGTTTGCACCTAAGGCGATTCTTTATTC
TAGCTGTAATCCCAAAACCTTAGCGAAAGATCTCGAGCATATTCAAGGTTATCACTTAACCAAGGTGCAGCTATTTGATT
TATTTCCCCACTCGGATCATTTTGAAGTTTTGGCTATGTTGGTGAAAGATTAG

Upstream 100 bases:

>100_bases
TAAAATTGCGCCCTTGGTACTGGTTGAGTGTGTTTCGAGATGATTTTTACCCGTAAAACCGCTACAGTGTGGCGCTTTTT
TACTGAATCTAGGATCTGTC

Downstream 100 bases:

>100_bases
TTTTTTAGCTCATCAATTTATCACTAAGCATTTGTACTCACTCGTTTTACGACTCTTGAAATCCGCTCAAACTATCTTAA
GGGCTCTTCTGTGTGGAGCC

Product: 23S rRNA methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U747)-methyltransferase [H]

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MSAAIVNAVKQCGYFNQGQCLSCRHIQQPLAQQVAVKTQTLQQLLAPFIPANSAELFLPPITGDDSGFRNKAKMVVLGAA
HEPVLGIVGPSGEAVDLCDCLLYPADMQALLHRLTRFVQQAGLPPYRVDKAKGELKFILLTRSQVRGEYLLRFVLRSHNG
IERIERELPALLAEYPQIKVVSVNIQPIHMAILEGDEEIFLTENTRLEERFNHVPLFIRPKSFFQTNPQVAAQLYQTARE
WVAEFSPRSLWDLFCGVGGFGLHCASNDITLTGIEIEAEAIACAQMSAQMMGLENVQFMALDSTDFAKGKSAADKPDLII
VNPPRRGIGEALCQSLSEFAPKAILYSSCNPKTLAKDLEHIQGYHLTKVQLFDLFPHSDHFEVLAMLVKD

Sequences:

>Translated_390_residues
MSAAIVNAVKQCGYFNQGQCLSCRHIQQPLAQQVAVKTQTLQQLLAPFIPANSAELFLPPITGDDSGFRNKAKMVVLGAA
HEPVLGIVGPSGEAVDLCDCLLYPADMQALLHRLTRFVQQAGLPPYRVDKAKGELKFILLTRSQVRGEYLLRFVLRSHNG
IERIERELPALLAEYPQIKVVSVNIQPIHMAILEGDEEIFLTENTRLEERFNHVPLFIRPKSFFQTNPQVAAQLYQTARE
WVAEFSPRSLWDLFCGVGGFGLHCASNDITLTGIEIEAEAIACAQMSAQMMGLENVQFMALDSTDFAKGKSAADKPDLII
VNPPRRGIGEALCQSLSEFAPKAILYSSCNPKTLAKDLEHIQGYHLTKVQLFDLFPHSDHFEVLAMLVKD
>Mature_389_residues
SAAIVNAVKQCGYFNQGQCLSCRHIQQPLAQQVAVKTQTLQQLLAPFIPANSAELFLPPITGDDSGFRNKAKMVVLGAAH
EPVLGIVGPSGEAVDLCDCLLYPADMQALLHRLTRFVQQAGLPPYRVDKAKGELKFILLTRSQVRGEYLLRFVLRSHNGI
ERIERELPALLAEYPQIKVVSVNIQPIHMAILEGDEEIFLTENTRLEERFNHVPLFIRPKSFFQTNPQVAAQLYQTAREW
VAEFSPRSLWDLFCGVGGFGLHCASNDITLTGIEIEAEAIACAQMSAQMMGLENVQFMALDSTDFAKGKSAADKPDLIIV
NPPRRGIGEALCQSLSEFAPKAILYSSCNPKTLAKDLEHIQGYHLTKVQLFDLFPHSDHFEVLAMLVKD

Specific function: Catalyzes the formation of 5-methyl-uridine at position 747 (M-5-U747) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. RumB subfamily [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=185, Percent_Identity=27.027027027027, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI51173878, Length=185, Percent_Identity=27.027027027027, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI269784680, Length=190, Percent_Identity=28.9473684210526, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI269784676, Length=190, Percent_Identity=28.9473684210526, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI20149688, Length=190, Percent_Identity=28.9473684210526, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI269784678, Length=190, Percent_Identity=28.9473684210526, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1787083, Length=379, Percent_Identity=54.353562005277, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI1789148, Length=181, Percent_Identity=30.939226519337, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790403, Length=182, Percent_Identity=29.6703296703297, Blast_Score=80, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24666579, Length=362, Percent_Identity=24.585635359116, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011825
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.189 [H]

Molecular weight: Translated: 43301; Mature: 43169

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAIVNAVKQCGYFNQGQCLSCRHIQQPLAQQVAVKTQTLQQLLAPFIPANSAELFLPP
CCHHHHHHHHHCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECC
ITGDDSGFRNKAKMVVLGAAHEPVLGIVGPSGEAVDLCDCLLYPADMQALLHRLTRFVQQ
CCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
AGLPPYRVDKAKGELKFILLTRSQVRGEYLLRFVLRSHNGIERIERELPALLAEYPQIKV
CCCCCEEECCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEE
VSVNIQPIHMAILEGDEEIFLTENTRLEERFNHVPLFIRPKSFFQTNPQVAAQLYQTARE
EEEECCEEEEEEEECCCEEEEECCCCHHHHHCCCCEEECCHHHHCCCHHHHHHHHHHHHH
WVAEFSPRSLWDLFCGVGGFGLHCASNDITLTGIEIEAEAIACAQMSAQMMGLENVQFMA
HHHHCCCHHHHHHHHCCCCCEEEECCCCEEEEEEEEEHHHHHHHHHHHHHHCCCCEEEEE
LDSTDFAKGKSAADKPDLIIVNPPRRGIGEALCQSLSEFAPKAILYSSCNPKTLAKDLEH
ECCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHH
IQGYHLTKVQLFDLFPHSDHFEVLAMLVKD
HCCCCEEEEEEEECCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SAAIVNAVKQCGYFNQGQCLSCRHIQQPLAQQVAVKTQTLQQLLAPFIPANSAELFLPP
CHHHHHHHHHCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECC
ITGDDSGFRNKAKMVVLGAAHEPVLGIVGPSGEAVDLCDCLLYPADMQALLHRLTRFVQQ
CCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
AGLPPYRVDKAKGELKFILLTRSQVRGEYLLRFVLRSHNGIERIERELPALLAEYPQIKV
CCCCCEEECCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEE
VSVNIQPIHMAILEGDEEIFLTENTRLEERFNHVPLFIRPKSFFQTNPQVAAQLYQTARE
EEEECCEEEEEEEECCCEEEEECCCCHHHHHCCCCEEECCHHHHCCCHHHHHHHHHHHHH
WVAEFSPRSLWDLFCGVGGFGLHCASNDITLTGIEIEAEAIACAQMSAQMMGLENVQFMA
HHHHCCCHHHHHHHHCCCCCEEEECCCCEEEEEEEEEHHHHHHHHHHHHHHCCCCEEEEE
LDSTDFAKGKSAADKPDLIIVNPPRRGIGEALCQSLSEFAPKAILYSSCNPKTLAKDLEH
ECCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHH
IQGYHLTKVQLFDLFPHSDHFEVLAMLVKD
HCCCCEEEEEEEECCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA