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The map label for this gene is thrS [H]

Identifier: 126172877

GI number: 126172877

Start: 713476

End: 714687

Strand: Direct

Name: thrS [H]

Synonym: Sbal_0628

Alternate gene names: 126172877

Gene position: 713476-714687 (Clockwise)

Preceding gene: 126172876

Following gene: 126172878

Centisome position: 13.92

GC content: 45.87

Gene sequence:

>1212_bases
ATGAAAACACATCGCGAAATTGCAGAACAACTTGACCTGTTTCACTCTGAACCACAGGCACCAGGCATGATTTTTTGGCA
CCCTAATGGCTGGGCGTTATTCCGAGATATTCAAGAGCACATGAGGCGCGTGTATCGCCGAAATGGTTTTGAGGAAGTGA
ACACGCCTGTTTTTATGAAGAAGGAGTTATGGCAAACGTCTGGGCATATGGACATGTTTGCAGAGAACATGTTTTTCGGT
GGCGGAAAGGAAAACATCGATGAATATGTGTTAAAACCAATGTCTTGTCCTGCCCATATTTTGCTGTTTAAGCGCGGGGT
ACATAGCCATAGAGATTTGCCACTGCGACTATTTGAGTTTGGCTTGGTGCACCGTAATGAATCCTCCGGTTCGTTAAATG
GTTGCCTGCGCGCACGCCAGTTCACTCAGGATGATGCCCATGTTTTTTGCTGTTGGTCTCAGGCAGAGGAGGAGATACGT
GGTTTTCTCCAACGTGCAAAGCAGGTTTATGGTGATTACGGCTACCATAATTTAGACGTAAAAATATCGACTAAACCTGA
GAAGTCATTAGGCAGCGATGAGCAATGGTGCCGAGCACAAAAGCTATTGGATTCAGCATGTCATATTGAGGGCTTTGATG
CCGAGTATCAAGCTGGTGAAGGGGCTTTTTATGGTCCCAAAATTGAACTGTCACTGACAGATTCTATGGGAAGGCGCTGG
CAGTGTGGCACTATCCAGTTTGATTTCAATTTGCCTGAAAGATTCGATGTTCAGTACGCAAATGAGCAAGGTGAATTCGA
ACGGCCAGTGATTATGCATCAAGCTATGTATGGCTCTATCGAGCGTTGGTTGGGGATTCTCTTGGAAGTATCACAGGGTA
ACCTGCCGGTTTGGTTACATCCCGTACCTGTTGCTATCGCCTCAGTGAATGAGTCAAACAGCCAATTTGTCCGCCAGCTA
GCCACATTATGCTTAGCACGAGACATTCGAGTGTTGGTTGATATCAGTCAAAACTCAGTTGCCCGCAAAATGAAACGCTT
TCACAAGCAAAAAGTGCCTTACATCTTAACGGTTGGGGATAAGGAGGAGGCTGAAAATAGGGTTTCAGTTAAGTGCCGAA
AAGACGGCGTCATTACTCACGTCCCTCTTGCTGAATTAGCTGACTATCTCGCGGATAAGCTAGAAGGCAAGCTAAAACGT
AAGGAAGACTGA

Upstream 100 bases:

>100_bases
CTTTAAAAAAACCCTCTGAGCGACATCGCCAGAGGGTTTTTTTATACCTTGGTTTTGTATTTCGCTCAGATTTTACTCAG
ATTTAACTCAGGAGAGAATG

Downstream 100 bases:

>100_bases
GTCTGTTACCTAGGGCTAGCCATGGTTCCCATGCCTAGCCTTTTACTCATCTTGTTACTTCGTCTAACAATGAGTTGGCT
CATTAAAGGGGCAGTGACAC

Product: tRNA synthetase class II (G H P and S)

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MKTHREIAEQLDLFHSEPQAPGMIFWHPNGWALFRDIQEHMRRVYRRNGFEEVNTPVFMKKELWQTSGHMDMFAENMFFG
GGKENIDEYVLKPMSCPAHILLFKRGVHSHRDLPLRLFEFGLVHRNESSGSLNGCLRARQFTQDDAHVFCCWSQAEEEIR
GFLQRAKQVYGDYGYHNLDVKISTKPEKSLGSDEQWCRAQKLLDSACHIEGFDAEYQAGEGAFYGPKIELSLTDSMGRRW
QCGTIQFDFNLPERFDVQYANEQGEFERPVIMHQAMYGSIERWLGILLEVSQGNLPVWLHPVPVAIASVNESNSQFVRQL
ATLCLARDIRVLVDISQNSVARKMKRFHKQKVPYILTVGDKEEAENRVSVKCRKDGVITHVPLAELADYLADKLEGKLKR
KED

Sequences:

>Translated_403_residues
MKTHREIAEQLDLFHSEPQAPGMIFWHPNGWALFRDIQEHMRRVYRRNGFEEVNTPVFMKKELWQTSGHMDMFAENMFFG
GGKENIDEYVLKPMSCPAHILLFKRGVHSHRDLPLRLFEFGLVHRNESSGSLNGCLRARQFTQDDAHVFCCWSQAEEEIR
GFLQRAKQVYGDYGYHNLDVKISTKPEKSLGSDEQWCRAQKLLDSACHIEGFDAEYQAGEGAFYGPKIELSLTDSMGRRW
QCGTIQFDFNLPERFDVQYANEQGEFERPVIMHQAMYGSIERWLGILLEVSQGNLPVWLHPVPVAIASVNESNSQFVRQL
ATLCLARDIRVLVDISQNSVARKMKRFHKQKVPYILTVGDKEEAENRVSVKCRKDGVITHVPLAELADYLADKLEGKLKR
KED
>Mature_403_residues
MKTHREIAEQLDLFHSEPQAPGMIFWHPNGWALFRDIQEHMRRVYRRNGFEEVNTPVFMKKELWQTSGHMDMFAENMFFG
GGKENIDEYVLKPMSCPAHILLFKRGVHSHRDLPLRLFEFGLVHRNESSGSLNGCLRARQFTQDDAHVFCCWSQAEEEIR
GFLQRAKQVYGDYGYHNLDVKISTKPEKSLGSDEQWCRAQKLLDSACHIEGFDAEYQAGEGAFYGPKIELSLTDSMGRRW
QCGTIQFDFNLPERFDVQYANEQGEFERPVIMHQAMYGSIERWLGILLEVSQGNLPVWLHPVPVAIASVNESNSQFVRQL
ATLCLARDIRVLVDISQNSVARKMKRFHKQKVPYILTVGDKEEAENRVSVKCRKDGVITHVPLAELADYLADKLEGKLKR
KED

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=406, Percent_Identity=37.192118226601, Blast_Score=282, Evalue=5e-76,
Organism=Homo sapiens, GI38570111, Length=393, Percent_Identity=36.3867684478371, Blast_Score=277, Evalue=1e-74,
Organism=Homo sapiens, GI20070344, Length=419, Percent_Identity=34.6062052505967, Blast_Score=267, Evalue=2e-71,
Organism=Escherichia coli, GI1788013, Length=403, Percent_Identity=41.6873449131514, Blast_Score=357, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI71984184, Length=403, Percent_Identity=36.4764267990074, Blast_Score=282, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI71984192, Length=403, Percent_Identity=36.4764267990074, Blast_Score=282, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6322112, Length=378, Percent_Identity=38.8888888888889, Blast_Score=281, Evalue=9e-77,
Organism=Saccharomyces cerevisiae, GI6322654, Length=397, Percent_Identity=34.5088161209068, Blast_Score=256, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24583842, Length=391, Percent_Identity=38.618925831202, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI28574141, Length=391, Percent_Identity=38.618925831202, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24583839, Length=391, Percent_Identity=38.618925831202, Blast_Score=286, Evalue=3e-77,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 46555; Mature: 46555

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTHREIAEQLDLFHSEPQAPGMIFWHPNGWALFRDIQEHMRRVYRRNGFEEVNTPVFMK
CCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCHHCCCCEEEH
KELWQTSGHMDMFAENMFFGGGKENIDEYVLKPMSCPAHILLFKRGVHSHRDLPLRLFEF
HHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHH
GLVHRNESSGSLNGCLRARQFTQDDAHVFCCWSQAEEEIRGFLQRAKQVYGDYGYHNLDV
HHEECCCCCCCHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
KISTKPEKSLGSDEQWCRAQKLLDSACHIEGFDAEYQAGEGAFYGPKIELSLTDSMGRRW
EEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCEEEEEEECCCCCEE
QCGTIQFDFNLPERFDVQYANEQGEFERPVIMHQAMYGSIERWLGILLEVSQGNLPVWLH
ECEEEEEECCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCEEEE
PVPVAIASVNESNSQFVRQLATLCLARDIRVLVDISQNSVARKMKRFHKQKVPYILTVGD
CCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEECC
KEEAENRVSVKCRKDGVITHVPLAELADYLADKLEGKLKRKED
HHHCCCCEEEEEECCCCEEECCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKTHREIAEQLDLFHSEPQAPGMIFWHPNGWALFRDIQEHMRRVYRRNGFEEVNTPVFMK
CCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCHHCCCCEEEH
KELWQTSGHMDMFAENMFFGGGKENIDEYVLKPMSCPAHILLFKRGVHSHRDLPLRLFEF
HHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHH
GLVHRNESSGSLNGCLRARQFTQDDAHVFCCWSQAEEEIRGFLQRAKQVYGDYGYHNLDV
HHEECCCCCCCHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
KISTKPEKSLGSDEQWCRAQKLLDSACHIEGFDAEYQAGEGAFYGPKIELSLTDSMGRRW
EEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCEEEEEEECCCCCEE
QCGTIQFDFNLPERFDVQYANEQGEFERPVIMHQAMYGSIERWLGILLEVSQGNLPVWLH
ECEEEEEECCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCEEEE
PVPVAIASVNESNSQFVRQLATLCLARDIRVLVDISQNSVARKMKRFHKQKVPYILTVGD
CCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEECC
KEEAENRVSVKCRKDGVITHVPLAELADYLADKLEGKLKRKED
HHHCCCCEEEEEECCCCEEECCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA