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The map label for this gene is nemA [H]

Identifier: 126172876

GI number: 126172876

Start: 712052

End: 713161

Strand: Direct

Name: nemA [H]

Synonym: Sbal_0627

Alternate gene names: 126172876

Gene position: 712052-713161 (Clockwise)

Preceding gene: 126172875

Following gene: 126172877

Centisome position: 13.89

GC content: 47.3

Gene sequence:

>1110_bases
ATGTTTATGATAGATAACAGCACACGTACAGACTTATTTTCAGCGACTCAACTGGGAAAAATTGCCATTGCGAATCGGAT
TATCATGGCGCCTGTCACTCGCAGTCGCTATGCCGAGAACGGTATCCCCAACGAATTACATGCAACCTATTATGCACAAC
GAGCCAGTGCCGGAATGTTGATCGCTGAGGCAACCAATATTTCACCTCAAGGCCGAGGTTATGCCGCCACCCCCGGTATT
TGGAGTGATGAGCAAGTCGAAGGTTGGAAGAAAGTCACGGATGCGGTTCATGCAAATGGCGGGAAAATTGTGGTCCAGCT
ATGGCACGTCGGCCGATTCTCATGTGTTGAGCTTCAACCCGATGGCGCTGCTCCCGTCGCACCCTCGGCGATTAAAGCGG
AAGGTAGCACTTATACTGTCGATGGTTTTGTGCCCGTTTCTATGCCACGGGCATTAGAAACAGACGAAATACCTAGGATT
ATCGAGCAATATAAAATAGCCGCAAGAAATGCTATGCGTGCTGGTTTCGATGGCGTCGAAGTGCATTCAGCTAACAGTTA
TTTACTCGACCAATTTTTACGAGATTCAACGAACCATCGCACAGACGAATATGGTGGCTCGATTGAAAACCGTGCCCGTT
TAACGTTAGAAGTGACTCAAGCCGTGGTTAATATCTGGGGCAGTGACCGAGTCGGCATTCGTCTATCGCCAGTCACTCCT
GATGCAGGTAATACCCCACCAGACAGTAATGTCATGGCTCTGTATGGCTACTTAATTCAGCAGCTTAACCAGTTTAATCT
TGCGTATCTGCACTTTGTTGAAGGTGCAACCGCCACCTCAAGAGAAGTGCCCGAAGGTGTGGATATGGATACATTAAGTG
TGCAATTTAATGGTCCATTTATTGGCAACAATAATTATGATCTCGAAATGGCCATTGAACGTCGTGCACAAGGAAAAATC
GATGCTGTCGCCTTCGGACGATTATTTATTTCCAATCCAGACTTAGTTGAGCGCTTACGTCGTGGTGCTGAACTCACCAT
AGCAGAACGTGAAAGCTACTATGGCGGCGGTGCAAAAGGCTATACCGATTGGCCAAAAGGCAACTATTAA

Upstream 100 bases:

>100_bases
ACATTTGCTCATTGAAGATTGATGAGATGTTCAGCCGCAACGGACGCATTAAATTCATTATTTTTTCAACCTTAAACTAG
ACGACTGGTCTACTAAAGAG

Downstream 100 bases:

>100_bases
TCTAACCGCCCATTCTTTATAAAACCACGCGATTAACCGTTTATGGGAGACAGGCAAGTGACTAGAAGCTAACGCGATAT
TGTGGAATTTCTAAACAAAA

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MFMIDNSTRTDLFSATQLGKIAIANRIIMAPVTRSRYAENGIPNELHATYYAQRASAGMLIAEATNISPQGRGYAATPGI
WSDEQVEGWKKVTDAVHANGGKIVVQLWHVGRFSCVELQPDGAAPVAPSAIKAEGSTYTVDGFVPVSMPRALETDEIPRI
IEQYKIAARNAMRAGFDGVEVHSANSYLLDQFLRDSTNHRTDEYGGSIENRARLTLEVTQAVVNIWGSDRVGIRLSPVTP
DAGNTPPDSNVMALYGYLIQQLNQFNLAYLHFVEGATATSREVPEGVDMDTLSVQFNGPFIGNNNYDLEMAIERRAQGKI
DAVAFGRLFISNPDLVERLRRGAELTIAERESYYGGGAKGYTDWPKGNY

Sequences:

>Translated_369_residues
MFMIDNSTRTDLFSATQLGKIAIANRIIMAPVTRSRYAENGIPNELHATYYAQRASAGMLIAEATNISPQGRGYAATPGI
WSDEQVEGWKKVTDAVHANGGKIVVQLWHVGRFSCVELQPDGAAPVAPSAIKAEGSTYTVDGFVPVSMPRALETDEIPRI
IEQYKIAARNAMRAGFDGVEVHSANSYLLDQFLRDSTNHRTDEYGGSIENRARLTLEVTQAVVNIWGSDRVGIRLSPVTP
DAGNTPPDSNVMALYGYLIQQLNQFNLAYLHFVEGATATSREVPEGVDMDTLSVQFNGPFIGNNNYDLEMAIERRAQGKI
DAVAFGRLFISNPDLVERLRRGAELTIAERESYYGGGAKGYTDWPKGNY
>Mature_369_residues
MFMIDNSTRTDLFSATQLGKIAIANRIIMAPVTRSRYAENGIPNELHATYYAQRASAGMLIAEATNISPQGRGYAATPGI
WSDEQVEGWKKVTDAVHANGGKIVVQLWHVGRFSCVELQPDGAAPVAPSAIKAEGSTYTVDGFVPVSMPRALETDEIPRI
IEQYKIAARNAMRAGFDGVEVHSANSYLLDQFLRDSTNHRTDEYGGSIENRARLTLEVTQAVVNIWGSDRVGIRLSPVTP
DAGNTPPDSNVMALYGYLIQQLNQFNLAYLHFVEGATATSREVPEGVDMDTLSVQFNGPFIGNNNYDLEMAIERRAQGKI
DAVAFGRLFISNPDLVERLRRGAELTIAERESYYGGGAKGYTDWPKGNY

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=361, Percent_Identity=52.3545706371191, Blast_Score=342, Evalue=3e-95,
Organism=Escherichia coli, GI1789463, Length=228, Percent_Identity=35.0877192982456, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17559802, Length=274, Percent_Identity=31.7518248175182, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17565138, Length=218, Percent_Identity=30.2752293577982, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17540738, Length=256, Percent_Identity=28.125, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17559804, Length=135, Percent_Identity=35.5555555555556, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI72001454, Length=342, Percent_Identity=25.7309941520468, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17564188, Length=215, Percent_Identity=27.4418604651163, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17566914, Length=213, Percent_Identity=28.169014084507, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325086, Length=374, Percent_Identity=40.1069518716578, Blast_Score=232, Evalue=8e-62,
Organism=Saccharomyces cerevisiae, GI6321973, Length=377, Percent_Identity=38.1962864721485, Blast_Score=218, Evalue=1e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40489; Mature: 40489

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFMIDNSTRTDLFSATQLGKIAIANRIIMAPVTRSRYAENGIPNELHATYYAQRASAGML
CEEECCCCCCHHHHHHHHCHHHHHCEEEECCCHHHHHHCCCCCCHHHHHHHHHHCCCCEE
IAEATNISPQGRGYAATPGIWSDEQVEGWKKVTDAVHANGGKIVVQLWHVGRFSCVELQP
EEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEECC
DGAAPVAPSAIKAEGSTYTVDGFVPVSMPRALETDEIPRIIEQYKIAARNAMRAGFDGVE
CCCCCCCCCCEECCCCEEEEECEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEE
VHSANSYLLDQFLRDSTNHRTDEYGGSIENRARLTLEVTQAVVNIWGSDRVGIRLSPVTP
EECCCHHHHHHHHHCCCCCCCHHHCCCCCCCEEEEHHHHHHHHHHCCCCCCCEEEECCCC
DAGNTPPDSNVMALYGYLIQQLNQFNLAYLHFVEGATATSREVPEGVDMDTLSVQFNGPF
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCE
IGNNNYDLEMAIERRAQGKIDAVAFGRLFISNPDLVERLRRGAELTIAERESYYGGGAKG
ECCCCCEEEEEEEHHCCCCEEEEEEEEEEECCCHHHHHHHCCCEEEEEECCCCCCCCCCC
YTDWPKGNY
CCCCCCCCC
>Mature Secondary Structure
MFMIDNSTRTDLFSATQLGKIAIANRIIMAPVTRSRYAENGIPNELHATYYAQRASAGML
CEEECCCCCCHHHHHHHHCHHHHHCEEEECCCHHHHHHCCCCCCHHHHHHHHHHCCCCEE
IAEATNISPQGRGYAATPGIWSDEQVEGWKKVTDAVHANGGKIVVQLWHVGRFSCVELQP
EEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEECC
DGAAPVAPSAIKAEGSTYTVDGFVPVSMPRALETDEIPRIIEQYKIAARNAMRAGFDGVE
CCCCCCCCCCEECCCCEEEEECEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEE
VHSANSYLLDQFLRDSTNHRTDEYGGSIENRARLTLEVTQAVVNIWGSDRVGIRLSPVTP
EECCCHHHHHHHHHCCCCCCCHHHCCCCCCCEEEEHHHHHHHHHHCCCCCCCEEEECCCC
DAGNTPPDSNVMALYGYLIQQLNQFNLAYLHFVEGATATSREVPEGVDMDTLSVQFNGPF
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCE
IGNNNYDLEMAIERRAQGKIDAVAFGRLFISNPDLVERLRRGAELTIAERESYYGGGAKG
ECCCCCEEEEEEEHHCCCCEEEEEEEEEEECCCHHHHHHHCCCEEEEEECCCCCCCCCCC
YTDWPKGNY
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]