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The map label for this gene is fixJ [H]

Identifier: 126172842

GI number: 126172842

Start: 674280

End: 674900

Strand: Direct

Name: fixJ [H]

Synonym: Sbal_0593

Alternate gene names: 126172842

Gene position: 674280-674900 (Clockwise)

Preceding gene: 126172841

Following gene: 126172843

Centisome position: 13.15

GC content: 51.37

Gene sequence:

>621_bases
ATGAGTCTTGAGTTACCTGTGTATTTAATCGATGACGATGACTCAGTGCGTCGTTCTTTGCGTTTTATGCTTGAAAGTTA
TGGCCTCAAGATCACCGACTTTGATAGCGCCGAAGCCTTTTTTACCGCCGTAGACTTAACCCTGCCGGGTTGTGCGCTGG
TTGATGTGCGTATGCCGGGCTTGAGCGGCCCGCAGTTGCATCTTGAATTAGTCGCCAAAAATAGTCCGTTAGCTGTGATT
TATTTGACCGGCCATGGCGATGTGCCTATGGCGGTAGAAGCGCTCAAACTCGGTGCGGTGGATTTTTTCCAAAAGCCCGC
CGATGGCGCAAAGTTAGCCGAAGCGGTACTCAAAGCGTTGGAACATACCAAGGCGCATCATCAAGACAATCAGTATCTTG
AGACTTATCAAGCGCTCACGCCAAGGGAGCGGGAGATACTCAACCTGATTGCCCAAGGCCTGAAGAACCAAGAGATCGCC
GATAGCCTCTGCATTGCCATGCGTACGGTCGAGATCCACCGTGCCAATTTGATGAAGGGCATGCAAGTAGGATCGCTGGC
GGAAATGATGCTGATCTATAGCCGGATCGCCGAACGCCTGCGCCTTGAAGATAACCGCTAA

Upstream 100 bases:

>100_bases
AGAGCCATGGCGGGACGTTTAGCCTAGAAAGCGCAATGGGTGGCGGTTGCCAAGCGCAGGTGGCTATACCGCGTAAACTT
GAACCCAATGGAGCCTAGTG

Downstream 100 bases:

>100_bases
AATTTACCTTTTACTGCGCTAATTGCCGCTTTGTTAGCCGCCTATTTTGGCTAAGTTGTCGTATCATTGGCGGTTAGTAT
TGGCTTTGCTAGTAGCTTAG

Product: response regulator receiver protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MSLELPVYLIDDDDSVRRSLRFMLESYGLKITDFDSAEAFFTAVDLTLPGCALVDVRMPGLSGPQLHLELVAKNSPLAVI
YLTGHGDVPMAVEALKLGAVDFFQKPADGAKLAEAVLKALEHTKAHHQDNQYLETYQALTPREREILNLIAQGLKNQEIA
DSLCIAMRTVEIHRANLMKGMQVGSLAEMMLIYSRIAERLRLEDNR

Sequences:

>Translated_206_residues
MSLELPVYLIDDDDSVRRSLRFMLESYGLKITDFDSAEAFFTAVDLTLPGCALVDVRMPGLSGPQLHLELVAKNSPLAVI
YLTGHGDVPMAVEALKLGAVDFFQKPADGAKLAEAVLKALEHTKAHHQDNQYLETYQALTPREREILNLIAQGLKNQEIA
DSLCIAMRTVEIHRANLMKGMQVGSLAEMMLIYSRIAERLRLEDNR
>Mature_205_residues
SLELPVYLIDDDDSVRRSLRFMLESYGLKITDFDSAEAFFTAVDLTLPGCALVDVRMPGLSGPQLHLELVAKNSPLAVIY
LTGHGDVPMAVEALKLGAVDFFQKPADGAKLAEAVLKALEHTKAHHQDNQYLETYQALTPREREILNLIAQGLKNQEIAD
SLCIAMRTVEIHRANLMKGMQVGSLAEMMLIYSRIAERLRLEDNR

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=135, Percent_Identity=34.0740740740741, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22952; Mature: 22821

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLELPVYLIDDDDSVRRSLRFMLESYGLKITDFDSAEAFFTAVDLTLPGCALVDVRMPG
CCCCCEEEEECCCHHHHHHHHHHHHHHCCEEEECCCCHHHEEEEEECCCCEEEEEEECCC
LSGPQLHLELVAKNSPLAVIYLTGHGDVPMAVEALKLGAVDFFQKPADGAKLAEAVLKAL
CCCCCEEEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
EHTKAHHQDNQYLETYQALTPREREILNLIAQGLKNQEIADSLCIAMRTVEIHRANLMKG
HHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
MQVGSLAEMMLIYSRIAERLRLEDNR
CCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SLELPVYLIDDDDSVRRSLRFMLESYGLKITDFDSAEAFFTAVDLTLPGCALVDVRMPG
CCCCEEEEECCCHHHHHHHHHHHHHHCCEEEECCCCHHHEEEEEECCCCEEEEEEECCC
LSGPQLHLELVAKNSPLAVIYLTGHGDVPMAVEALKLGAVDFFQKPADGAKLAEAVLKAL
CCCCCEEEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
EHTKAHHQDNQYLETYQALTPREREILNLIAQGLKNQEIADSLCIAMRTVEIHRANLMKG
HHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
MQVGSLAEMMLIYSRIAERLRLEDNR
CCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2046550 [H]