Click here to switch to the map view.

The map label for this gene is fixL [H]

Identifier: 126172841

GI number: 126172841

Start: 672343

End: 674277

Strand: Direct

Name: fixL [H]

Synonym: Sbal_0592

Alternate gene names: 126172841

Gene position: 672343-674277 (Clockwise)

Preceding gene: 126172836

Following gene: 126172842

Centisome position: 13.11

GC content: 52.87

Gene sequence:

>1935_bases
ATGGCACCTCGAATGATTTTAAGGATGGCACTAGCCGTGGTGTTAGCGTGCTTGCTCGCTTCGGCAAACGTGTTCGCCGC
CGAGCCGCAGAATCAAAGCGCGCCTTCGGATGGAATGGACGCAAAGGTTGTTGCGCCAGTCGCTACCGAAGAGCCCGGTT
ATCAAGTGGTCGATGTGGGTGTGTTAGCCATCCGCGGTTATAAGGCGACCATCAACCGCTGGCAGCCGTTAATGGGTTGG
CTCGAAACCCAAATTCCCAACAGTTACTTTAGGTTACATCCTCTCTCCCTCGATGAGCTCGCTAAGGGCGTTGAAACCCA
AGGGCTCGATTTTGTGATCACTAACCCCGGGCAATCCGTGTTGCTGGCGCGGCAATATTCTCTCACTTGGTTGGCGACCC
TACGCAGCCCGTTAAATAATGGCGCGGCGATGCAAGTGGGTTCGGCGCTGGTGGTGCGCGCCGATTCACCCTATCAGACT
TTAACTGATCTCAAGGGGCGTCGACTCGGTATAGTGTCGAAAAATGCCTTTGGCGGTTACTTGACCTTAGTGTACGAAGC
GCAGCTAAAGGGCATAGATTTGCCTCGCTTTGTGGGTGAGATTATTCCGCTGGGATTCCCGCTAGATAATCTGTTGTATC
AGTTAGATGACCAGAAAAGTGGTGATGATGCGGCGAAAGACAATAGGCTGGATGCGGCTGTAGTGCCTGTGTGTCAGCTT
GAGCAGATGCAGGCAGAAGGGCTGATTAATATCGGCCATTATCGGGTGCTCGATAACCAAACTCCGGTGGGATTTCATTG
CCAAGTCTCGACTCGTCTCTATCCCAACTGGTCCATGGCGAAGACCAATCGCGCCTCGCAATCTTTAGCTAAAAGCGTGA
CTCAAGCGCTGCTGGCACTGCCCGAAGATCACCTCGCCGCTAAGACGGCCGATTCTGCGGGTTGGACCACAGCCGTGAGT
CAGCTGGCGATTGATCAGTTACTTAAAGATCTCGACATGCATCCCCTGCAAACACCTTGGTGGCAAAGGGCGTGGCAGTG
GGTCAAGTTACATCAACAATGGGCTTGGTTTGTGCTGGCGATTCTGGTGCTGCTCAATGCGTACCATTTCTGGTTAGAGT
ATCGCTTTAGCCGTCGCGGCCGCGAGTTGATTAATACTCAGCGCCAGCTCAATGAAAACCGTGCGTTACTGGAACATGCC
CAGCGCATCGCGATTGCTGGCGAACTTGGCGCGAGTCTCTCCCACGAACTGAATCAACCTTTGGCGGCGATTGGCCATTA
TTGTCATGGCGCCGAAGTGCGATTACAGCGGGGTACTAGCCCTGAGGAACTGCAATCGGTATTGACGTTGATCCAGCAGG
AAGTCACGCGAGCCGACAGCATTATCAGCCGCTTACGCAACCTGTTGAAGAAACGGCCTGTATCGAAACAACCTTTGTAT
TTGCATGAGCTGGTCAATGATACTGTGCCGCTGCTCGCCTATGAGTTTGAACAACATCAAATTAATCTTGCCGTAAACGT
GAGCGGCGAGCCCTATTTACAGTCCCTCGATGAAGTCGGCATGCAGCAGTTGTTGCTTAACCTGCTGAAAAACGCCGCCG
ATGCCTGTGTTCAGCGGCTAGAGCTTGAATCCAGTGGTACCGAACAGATCATCACGCAAAAGCCATACACGCCGACGATC
GATATCGATCTTCGCTACCAAGAACGCATACTCTTGCTGACAGTCACGGATAATGGCACGGGTTTAACCGAAGCCGCCAG
CCTGCTGATGCAAGCCTTTTATTCCACTAAATCAGAGGGATTAGGTTTAGGCTTAGTGATTTGCCGCGATATTGCCGAGA
GCCATGGCGGGACGTTTAGCCTAGAAAGCGCAATGGGTGGCGGTTGCCAAGCGCAGGTGGCTATACCGCGTAAACTTGAA
CCCAATGGAGCCTAG

Upstream 100 bases:

>100_bases
TAAAGGATTGCACTTCACGTTATTTAGGATGATGAAAATAGTGTTTAGCTCAGGGATGTCGCTCAAGCGGCCATTATCGA
TTCAAGGAACAGTGCCTAAG

Downstream 100 bases:

>100_bases
TGATGAGTCTTGAGTTACCTGTGTATTTAATCGATGACGATGACTCAGTGCGTCGTTCTTTGCGTTTTATGCTTGAAAGT
TATGGCCTCAAGATCACCGA

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 644; Mature: 643

Protein sequence:

>644_residues
MAPRMILRMALAVVLACLLASANVFAAEPQNQSAPSDGMDAKVVAPVATEEPGYQVVDVGVLAIRGYKATINRWQPLMGW
LETQIPNSYFRLHPLSLDELAKGVETQGLDFVITNPGQSVLLARQYSLTWLATLRSPLNNGAAMQVGSALVVRADSPYQT
LTDLKGRRLGIVSKNAFGGYLTLVYEAQLKGIDLPRFVGEIIPLGFPLDNLLYQLDDQKSGDDAAKDNRLDAAVVPVCQL
EQMQAEGLINIGHYRVLDNQTPVGFHCQVSTRLYPNWSMAKTNRASQSLAKSVTQALLALPEDHLAAKTADSAGWTTAVS
QLAIDQLLKDLDMHPLQTPWWQRAWQWVKLHQQWAWFVLAILVLLNAYHFWLEYRFSRRGRELINTQRQLNENRALLEHA
QRIAIAGELGASLSHELNQPLAAIGHYCHGAEVRLQRGTSPEELQSVLTLIQQEVTRADSIISRLRNLLKKRPVSKQPLY
LHELVNDTVPLLAYEFEQHQINLAVNVSGEPYLQSLDEVGMQQLLLNLLKNAADACVQRLELESSGTEQIITQKPYTPTI
DIDLRYQERILLLTVTDNGTGLTEAASLLMQAFYSTKSEGLGLGLVICRDIAESHGGTFSLESAMGGGCQAQVAIPRKLE
PNGA

Sequences:

>Translated_644_residues
MAPRMILRMALAVVLACLLASANVFAAEPQNQSAPSDGMDAKVVAPVATEEPGYQVVDVGVLAIRGYKATINRWQPLMGW
LETQIPNSYFRLHPLSLDELAKGVETQGLDFVITNPGQSVLLARQYSLTWLATLRSPLNNGAAMQVGSALVVRADSPYQT
LTDLKGRRLGIVSKNAFGGYLTLVYEAQLKGIDLPRFVGEIIPLGFPLDNLLYQLDDQKSGDDAAKDNRLDAAVVPVCQL
EQMQAEGLINIGHYRVLDNQTPVGFHCQVSTRLYPNWSMAKTNRASQSLAKSVTQALLALPEDHLAAKTADSAGWTTAVS
QLAIDQLLKDLDMHPLQTPWWQRAWQWVKLHQQWAWFVLAILVLLNAYHFWLEYRFSRRGRELINTQRQLNENRALLEHA
QRIAIAGELGASLSHELNQPLAAIGHYCHGAEVRLQRGTSPEELQSVLTLIQQEVTRADSIISRLRNLLKKRPVSKQPLY
LHELVNDTVPLLAYEFEQHQINLAVNVSGEPYLQSLDEVGMQQLLLNLLKNAADACVQRLELESSGTEQIITQKPYTPTI
DIDLRYQERILLLTVTDNGTGLTEAASLLMQAFYSTKSEGLGLGLVICRDIAESHGGTFSLESAMGGGCQAQVAIPRKLE
PNGA
>Mature_643_residues
APRMILRMALAVVLACLLASANVFAAEPQNQSAPSDGMDAKVVAPVATEEPGYQVVDVGVLAIRGYKATINRWQPLMGWL
ETQIPNSYFRLHPLSLDELAKGVETQGLDFVITNPGQSVLLARQYSLTWLATLRSPLNNGAAMQVGSALVVRADSPYQTL
TDLKGRRLGIVSKNAFGGYLTLVYEAQLKGIDLPRFVGEIIPLGFPLDNLLYQLDDQKSGDDAAKDNRLDAAVVPVCQLE
QMQAEGLINIGHYRVLDNQTPVGFHCQVSTRLYPNWSMAKTNRASQSLAKSVTQALLALPEDHLAAKTADSAGWTTAVSQ
LAIDQLLKDLDMHPLQTPWWQRAWQWVKLHQQWAWFVLAILVLLNAYHFWLEYRFSRRGRELINTQRQLNENRALLEHAQ
RIAIAGELGASLSHELNQPLAAIGHYCHGAEVRLQRGTSPEELQSVLTLIQQEVTRADSIISRLRNLLKKRPVSKQPLYL
HELVNDTVPLLAYEFEQHQINLAVNVSGEPYLQSLDEVGMQQLLLNLLKNAADACVQRLELESSGTEQIITQKPYTPTID
IDLRYQERILLLTVTDNGTGLTEAASLLMQAFYSTKSEGLGLGLVICRDIAESHGGTFSLESAMGGGCQAQVAIPRKLEP
NGA

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=292, Percent_Identity=25.3424657534247, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788549, Length=253, Percent_Identity=26.4822134387352, Blast_Score=77, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 71228; Mature: 71096

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPRMILRMALAVVLACLLASANVFAAEPQNQSAPSDGMDAKVVAPVATEEPGYQVVDVG
CCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEE
VLAIRGYKATINRWQPLMGWLETQIPNSYFRLHPLSLDELAKGVETQGLDFVITNPGQSV
EEEEECCHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCCCCCEEEEECCCCCE
LLARQYSLTWLATLRSPLNNGAAMQVGSALVVRADSPYQTLTDLKGRRLGIVSKNAFGGY
EEEEHHHHHHHHHHHHHCCCCCCEEECCEEEEECCCCHHHHHHCCCCEEEEEECCCCCCE
LTLVYEAQLKGIDLPRFVGEIIPLGFPLDNLLYQLDDQKSGDDAAKDNRLDAAVVPVCQL
EEEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHCCHHHH
EQMQAEGLINIGHYRVLDNQTPVGFHCQVSTRLYPNWSMAKTNRASQSLAKSVTQALLAL
HHHHHCCCEECCEEEEECCCCCCCEEEEECCEECCCCCCHHCCHHHHHHHHHHHHHHHHC
PEDHLAAKTADSAGWTTAVSQLAIDQLLKDLDMHPLQTPWWQRAWQWVKLHQQWAWFVLA
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ILVLLNAYHFWLEYRFSRRGRELINTQRQLNENRALLEHAQRIAIAGELGASLSHELNQP
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHHHHCCC
LAAIGHYCHGAEVRLQRGTSPEELQSVLTLIQQEVTRADSIISRLRNLLKKRPVSKQPLY
HHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
LHELVNDTVPLLAYEFEQHQINLAVNVSGEPYLQSLDEVGMQQLLLNLLKNAADACVQRL
HHHHHCCCHHHHEEEECCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELESSGTEQIITQKPYTPTIDIDLRYQERILLLTVTDNGTGLTEAASLLMQAFYSTKSEG
HHCCCCCHHHHCCCCCCCEEEEEEEEECEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCC
LGLGLVICRDIAESHGGTFSLESAMGGGCQAQVAIPRKLEPNGA
CCCHHHHHHHHHHHCCCEEEEHHHCCCCCEEEEECCCCCCCCCC
>Mature Secondary Structure 
APRMILRMALAVVLACLLASANVFAAEPQNQSAPSDGMDAKVVAPVATEEPGYQVVDVG
CHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEE
VLAIRGYKATINRWQPLMGWLETQIPNSYFRLHPLSLDELAKGVETQGLDFVITNPGQSV
EEEEECCHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCCCCCEEEEECCCCCE
LLARQYSLTWLATLRSPLNNGAAMQVGSALVVRADSPYQTLTDLKGRRLGIVSKNAFGGY
EEEEHHHHHHHHHHHHHCCCCCCEEECCEEEEECCCCHHHHHHCCCCEEEEEECCCCCCE
LTLVYEAQLKGIDLPRFVGEIIPLGFPLDNLLYQLDDQKSGDDAAKDNRLDAAVVPVCQL
EEEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHCCHHHH
EQMQAEGLINIGHYRVLDNQTPVGFHCQVSTRLYPNWSMAKTNRASQSLAKSVTQALLAL
HHHHHCCCEECCEEEEECCCCCCCEEEEECCEECCCCCCHHCCHHHHHHHHHHHHHHHHC
PEDHLAAKTADSAGWTTAVSQLAIDQLLKDLDMHPLQTPWWQRAWQWVKLHQQWAWFVLA
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ILVLLNAYHFWLEYRFSRRGRELINTQRQLNENRALLEHAQRIAIAGELGASLSHELNQP
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHHHHCCC
LAAIGHYCHGAEVRLQRGTSPEELQSVLTLIQQEVTRADSIISRLRNLLKKRPVSKQPLY
HHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
LHELVNDTVPLLAYEFEQHQINLAVNVSGEPYLQSLDEVGMQQLLLNLLKNAADACVQRL
HHHHHCCCHHHHEEEECCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELESSGTEQIITQKPYTPTIDIDLRYQERILLLTVTDNGTGLTEAASLLMQAFYSTKSEG
HHCCCCCHHHHCCCCCCCEEEEEEEEECEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCC
LGLGLVICRDIAESHGGTFSLESAMGGGCQAQVAIPRKLEPNGA
CCCHHHHHHHHHHHCCCEEEEHHHCCCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275; 9860942; 10747783 [H]