BacMap

Definition	Lactococcus lactis subsp. cremoris MG1363, complete genome.
Accession	NC_009004
Length	2,529,478

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The map label for this gene is dnaJ [H]

Identifier: 125625257

GI number: 125625257

Start: 2456461

End: 2457600

Strand: Reverse

Name: dnaJ [H]

Synonym: llmg_2502

Alternate gene names: 125625257

Gene position: 2457600-2456461 (Counterclockwise)

Preceding gene: 125625258

Following gene: 125625256

Centisome position: 97.16

GC content: 41.4

Gene sequence:

>1140_bases
ATGAATAATACTGAATATTATGAACGACTGGGTGTTGATAAAAATGCCAGTCAAGATGAAATAAAAAAAGCTTATCGTAA
AATGTCCAAAAAATATCACCCCGATTTAAATAAAGAAGAGGGTGCTGAGGATAAGTACAAAGAGGTTCAAGAAGCTTACG
AAACGCTTTCTGATGAACAAAAACGAGCAGCCTATGACCAATATGGTGAAGCGGGAGCTAATGGTGGCTTTGGCGGCGGC
GGTTTCGGTGGAGCTTCTGGATTCTCAGGCTTTGGCGGAGCTTCTGGCGGTTTCGGTGGTTTTGAGGATATCTTCTCAAG
TTTCTTTGGTGGCGGAGGAGCGCAAGTTAATCCAAATGCACCACGTCAGGGTGATGATTTACAATATCGAATCAATTTAA
AATTTGAAGAGGCTATTTTCGGCGTTGAAAAGCAAGTGAAGTATAATCGTGAAGAACTCTGTCATACTTGTGGTGGCTCA
GGAGCGAAAGCTGGAACGCATCCGGAAACGTGTCATAAGTGTGGAGGTCGTGGACAAATTAATGTTGTTCGTGATACACC
GCTTGGTCGGATGCAAACTCAAACAACTTGTGATGTCTGTCATGGAACAGGTAAGGAAATTAAAGAAAAATGCACAACTT
GTCATGGTTCAGGTCATGAAAAAGTTGCACATACTGTTAAAGTGACTGTACCTGCTGGTGTTGAAACTGGTCAAAAAATG
CGTTTACAAGGCCAAGGGGATGCAGGTGTAAATGGTGGTCCTTACGGTGATTTGTATGTTGTCTTCCAAGTAGAAGCTTC
AGATAAATTTGAACGCGATGGCGCGGAAATCTACTATAAGATGCCAATGGACTTTGTTCAAGCGGCCTTAGGGGATGAAG
TTGAAGTTCCAACCGTACACGGTAATGTGAAATTAAAAATTCCTGCGGGAACACAAACTGGCGCTAATTTCCGTCTAAAA
GGAAAAGGAGCTCCAAAACTCCGTGGTTCTGGTAATGGTGATCAATATGTAATTATTAATATTGTCACTCCTAAGAACAT
GAACCAAGCTCAAAAAGAAGCTCTTCAAGCTTTTGCCAAGGCAAGTGGTATTGAAGTTTCTGGTTCTGGTAAAAAAGGAT
TCTTTGATAAGTTTAAATAA

Upstream 100 bases:

>100_bases
CTGATAAAAAGAGTGCTAATTTTTTTGCCAAAAGTGGAAAAACGTGGTAAAATAGTGCTATTGAAAAATTGATTTAGTAA
AGGAAGTGAGTAATTTAGAA

Downstream 100 bases:

>100_bases
GAAAAAGTCGCTTGATGCGACTTTTTCTTCTGTCAGTAATTTGATGATTCTGTTTACTGGTGATAGAGCTGTTAGTAATT
CTAGGGAGGAAAACAGGAAT

Product: chaperone protein DnaJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MNNTEYYERLGVDKNASQDEIKKAYRKMSKKYHPDLNKEEGAEDKYKEVQEAYETLSDEQKRAAYDQYGEAGANGGFGGG
GFGGASGFSGFGGASGGFGGFEDIFSSFFGGGGAQVNPNAPRQGDDLQYRINLKFEEAIFGVEKQVKYNREELCHTCGGS
GAKAGTHPETCHKCGGRGQINVVRDTPLGRMQTQTTCDVCHGTGKEIKEKCTTCHGSGHEKVAHTVKVTVPAGVETGQKM
RLQGQGDAGVNGGPYGDLYVVFQVEASDKFERDGAEIYYKMPMDFVQAALGDEVEVPTVHGNVKLKIPAGTQTGANFRLK
GKGAPKLRGSGNGDQYVIINIVTPKNMNQAQKEALQAFAKASGIEVSGSGKKGFFDKFK

Sequences:

>Translated_379_residues
MNNTEYYERLGVDKNASQDEIKKAYRKMSKKYHPDLNKEEGAEDKYKEVQEAYETLSDEQKRAAYDQYGEAGANGGFGGG
GFGGASGFSGFGGASGGFGGFEDIFSSFFGGGGAQVNPNAPRQGDDLQYRINLKFEEAIFGVEKQVKYNREELCHTCGGS
GAKAGTHPETCHKCGGRGQINVVRDTPLGRMQTQTTCDVCHGTGKEIKEKCTTCHGSGHEKVAHTVKVTVPAGVETGQKM
RLQGQGDAGVNGGPYGDLYVVFQVEASDKFERDGAEIYYKMPMDFVQAALGDEVEVPTVHGNVKLKIPAGTQTGANFRLK
GKGAPKLRGSGNGDQYVIINIVTPKNMNQAQKEALQAFAKASGIEVSGSGKKGFFDKFK
>Mature_379_residues
MNNTEYYERLGVDKNASQDEIKKAYRKMSKKYHPDLNKEEGAEDKYKEVQEAYETLSDEQKRAAYDQYGEAGANGGFGGG
GFGGASGFSGFGGASGGFGGFEDIFSSFFGGGGAQVNPNAPRQGDDLQYRINLKFEEAIFGVEKQVKYNREELCHTCGGS
GAKAGTHPETCHKCGGRGQINVVRDTPLGRMQTQTTCDVCHGTGKEIKEKCTTCHGSGHEKVAHTVKVTVPAGVETGQKM
RLQGQGDAGVNGGPYGDLYVVFQVEASDKFERDGAEIYYKMPMDFVQAALGDEVEVPTVHGNVKLKIPAGTQTGANFRLK
GKGAPKLRGSGNGDQYVIINIVTPKNMNQAQKEALQAFAKASGIEVSGSGKKGFFDKFK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, dnaK-independent fashion. Unfolded proteins bind initially to dnaJ; upon

COG id: COG0484

COG function: function code O; DnaJ-class molecular chaperone with C-terminal Zn finger domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 J domain [H]

Homologues:

Organism=Homo sapiens, GI205360840, Length=383, Percent_Identity=35.509138381201, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI205360838, Length=381, Percent_Identity=35.4330708661417, Blast_Score=214, Evalue=8e-56,
Organism=Homo sapiens, GI194328758, Length=306, Percent_Identity=34.3137254901961, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI194328760, Length=306, Percent_Identity=34.3137254901961, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI5031741, Length=357, Percent_Identity=32.4929971988796, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI4504511, Length=302, Percent_Identity=33.7748344370861, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI201862587, Length=366, Percent_Identity=30.0546448087432, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI56549115, Length=372, Percent_Identity=29.8387096774194, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI201862518, Length=366, Percent_Identity=30.0546448087432, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI6631085, Length=365, Percent_Identity=28.7671232876712, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI194328762, Length=266, Percent_Identity=30.4511278195489, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI7706495, Length=381, Percent_Identity=27.8215223097113, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI39204547, Length=369, Percent_Identity=22.7642276422764, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI5453690, Length=320, Percent_Identity=26.875, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI47777312, Length=110, Percent_Identity=49.0909090909091, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI88501736, Length=109, Percent_Identity=48.6238532110092, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI27151736, Length=109, Percent_Identity=48.6238532110092, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI56687498, Length=68, Percent_Identity=57.3529411764706, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI23503241, Length=108, Percent_Identity=46.2962962962963, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI68077166, Length=104, Percent_Identity=42.3076923076923, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI68077168, Length=104, Percent_Identity=42.3076923076923, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI21553335, Length=75, Percent_Identity=56, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI9558755, Length=103, Percent_Identity=44.6601941747573, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI17388799, Length=110, Percent_Identity=50.9090909090909, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI194306642, Length=113, Percent_Identity=39.8230088495575, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI194306640, Length=113, Percent_Identity=39.8230088495575, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI4885495, Length=110, Percent_Identity=50.9090909090909, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI45504382, Length=67, Percent_Identity=52.2388059701493, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI22749377, Length=106, Percent_Identity=38.6792452830189, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI72534740, Length=108, Percent_Identity=41.6666666666667, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI217035105, Length=76, Percent_Identity=43.421052631579, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI29126218, Length=102, Percent_Identity=37.2549019607843, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1786197, Length=384, Percent_Identity=45.3125, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI1787235, Length=308, Percent_Identity=27.9220779220779, Blast_Score=111, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17553096, Length=373, Percent_Identity=35.656836461126, Blast_Score=187, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI17553098, Length=373, Percent_Identity=35.656836461126, Blast_Score=187, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI17507263, Length=355, Percent_Identity=32.9577464788732, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17534355, Length=368, Percent_Identity=30.1630434782609, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17563890, Length=169, Percent_Identity=32.5443786982249, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17538236, Length=113, Percent_Identity=42.4778761061947, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI212645553, Length=70, Percent_Identity=51.4285714285714, Blast_Score=78, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI115532378, Length=100, Percent_Identity=38, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17554644, Length=125, Percent_Identity=36, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI115532476, Length=63, Percent_Identity=42.8571428571429, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17568643, Length=68, Percent_Identity=48.5294117647059, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6324265, Length=351, Percent_Identity=36.1823361823362, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6321094, Length=396, Percent_Identity=35.3535353535354, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6323870, Length=356, Percent_Identity=34.2696629213483, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6324252, Length=430, Percent_Identity=27.4418604651163, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323119, Length=388, Percent_Identity=27.0618556701031, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323813, Length=108, Percent_Identity=46.2962962962963, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6322194, Length=111, Percent_Identity=44.1441441441441, Blast_Score=73, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6324321, Length=73, Percent_Identity=49.3150684931507, Blast_Score=73, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6324103, Length=72, Percent_Identity=50, Blast_Score=66, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6322388, Length=107, Percent_Identity=38.3177570093458, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320888, Length=109, Percent_Identity=37.6146788990826, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI45552813, Length=376, Percent_Identity=35.3723404255319, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI45549272, Length=376, Percent_Identity=35.3723404255319, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI45552811, Length=376, Percent_Identity=35.3723404255319, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI20129487, Length=354, Percent_Identity=31.9209039548023, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24584014, Length=354, Percent_Identity=31.9209039548023, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24646562, Length=391, Percent_Identity=29.156010230179, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24646560, Length=391, Percent_Identity=29.156010230179, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24646558, Length=391, Percent_Identity=29.156010230179, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24646556, Length=391, Percent_Identity=29.156010230179, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI21357547, Length=391, Percent_Identity=29.156010230179, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI85726398, Length=370, Percent_Identity=30.8108108108108, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI28574949, Length=359, Percent_Identity=26.4623955431755, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24658562, Length=376, Percent_Identity=28.7234042553192, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24658555, Length=376, Percent_Identity=28.7234042553192, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI19920464, Length=371, Percent_Identity=29.3800539083558, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI18858081, Length=361, Percent_Identity=25.4847645429363, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24654066, Length=119, Percent_Identity=37.8151260504202, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24654070, Length=119, Percent_Identity=37.8151260504202, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24654068, Length=119, Percent_Identity=37.8151260504202, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24654072, Length=119, Percent_Identity=37.8151260504202, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24668492, Length=68, Percent_Identity=48.5294117647059, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17737735, Length=67, Percent_Identity=53.7313432835821, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28574723, Length=67, Percent_Identity=53.7313432835821, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24668523, Length=67, Percent_Identity=53.7313432835821, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24762799, Length=111, Percent_Identity=36.9369369369369, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21358273, Length=119, Percent_Identity=39.4957983193277, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI161077138, Length=65, Percent_Identity=46.1538461538462, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012724
- InterPro:   IPR002939
- InterPro:   IPR001623
- InterPro:   IPR018253
- InterPro:   IPR008971
- InterPro:   IPR015609
- InterPro:   IPR003095
- InterPro:   IPR001305 [H]

Pfam domain/function: PF00226 DnaJ; PF01556 DnaJ_C; PF00684 DnaJ_CXXCXGXG [H]

EC number: NA

Molecular weight: Translated: 40687; Mature: 40687

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: PS00636 DNAJ_1 ; PS50076 DNAJ_2 ; PS00637 DNAJ_CXXCXGXG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNTEYYERLGVDKNASQDEIKKAYRKMSKKYHPDLNKEEGAEDKYKEVQEAYETLSDEQ
CCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
KRAAYDQYGEAGANGGFGGGGFGGASGFSGFGGASGGFGGFEDIFSSFFGGGGAQVNPNA
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEECCCC
PRQGDDLQYRINLKFEEAIFGVEKQVKYNREELCHTCGGSGAKAGTHPETCHKCGGRGQI
CCCCCCEEEEEEEEHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCHHHHHHCCCCCEE
NVVRDTPLGRMQTQTTCDVCHGTGKEIKEKCTTCHGSGHEKVAHTVKVTVPAGVETGQKM
EEEECCCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCEE
RLQGQGDAGVNGGPYGDLYVVFQVEASDKFERDGAEIYYKMPMDFVQAALGDEVEVPTVH
EEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEECCHHHHHHHHCCCCCCEEEE
GNVKLKIPAGTQTGANFRLKGKGAPKLRGSGNGDQYVIINIVTPKNMNQAQKEALQAFAK
CCEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHH
ASGIEVSGSGKKGFFDKFK
HCCEEEECCCCCCCCCCCC
>Mature Secondary Structure
MNNTEYYERLGVDKNASQDEIKKAYRKMSKKYHPDLNKEEGAEDKYKEVQEAYETLSDEQ
CCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
KRAAYDQYGEAGANGGFGGGGFGGASGFSGFGGASGGFGGFEDIFSSFFGGGGAQVNPNA
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEECCCC
PRQGDDLQYRINLKFEEAIFGVEKQVKYNREELCHTCGGSGAKAGTHPETCHKCGGRGQI
CCCCCCEEEEEEEEHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCHHHHHHCCCCCEE
NVVRDTPLGRMQTQTTCDVCHGTGKEIKEKCTTCHGSGHEKVAHTVKVTVPAGVETGQKM
EEEECCCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCEE
RLQGQGDAGVNGGPYGDLYVVFQVEASDKFERDGAEIYYKMPMDFVQAALGDEVEVPTVH
EEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEECCHHHHHHHHCCCCCCEEEE
GNVKLKIPAGTQTGANFRLKGKGAPKLRGSGNGDQYVIINIVTPKNMNQAQKEALQAFAK
CCEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHH
ASGIEVSGSGKKGFFDKFK
HCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8449872; 11337471 [H]