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The map label for this gene is glpF3 [H]

Identifier: 125625088

GI number: 125625088

Start: 2284047

End: 2284916

Strand: Reverse

Name: glpF3 [H]

Synonym: llmg_2327

Alternate gene names: 125625088

Gene position: 2284916-2284047 (Counterclockwise)

Preceding gene: 125625092

Following gene: 125625086

Centisome position: 90.33

GC content: 40.11

Gene sequence:

>870_bases
ATGGATGTTACATGGACAGTGAAATATATCACTGAATTTGTTGGAACTGCCCTACTCATTATTATGGGTAATGGTGCGGT
TGCTAACGTTGAACTTAAAGGTACAAAAGCTCACGCACAATCTTGGATGATTATTGGTTGGGGTTACGGACTTGGCGTAA
TGTTGCCAGCAGTTGCTTTTGGTAATATTACTTCACAAATCAACCCAGCTTTCACACTTGGACTTGCAGCCTCAGGACTT
TTTCCTTGGGCACACGTTGCTCAATACATTATTGCGCAAGTATTAGGTGCGATGTTTGGACAATTATTAATCGTTATGGT
TTATCGTCCATACTATCTTAAAACACAAAATCCAAATGCTATTCTTGGAACTTTCTCAACTATCGACAACGTTGATGATA
ACAGTGAAAAAACTCGTTTGGGGGCAACCATTAATGGTTTCTTGAATGAGTTCTTAGGTTCATTTGTATTATTCTTCGGT
GCGGTTGCTGCGACAAATATCTTCTTTGGTAGTCAATCAATTACTTGGATGACAAACTACTTGAAAGGTCAAGGAGCAGA
TGTTTCTTCATCAGACGTTATGAATCAAATTTGGGTTCAAGCATCAGGAGCTTCAGCTTCTAAAATGATTGCTCACTTGT
TCCTAGGATTCCTTGTTATGGGACTCGTAGTTGCACTTGGTGGACCTACAGGTCCTGGTTTGAATCCGGCGCGTGACTTT
GGTCCACGTTTGGTTCACAGTCTCTTACCAAAATCAGTTTTGGGTGAAGCTAAAGGTTCATCTAAATGGTGGTATGCATG
GGTTCCAGTTTTGGCACCAATTCTTGCATCACTTGCAGCCGTTGCATTATTTAAAATGATTTATCTTTAA

Upstream 100 bases:

>100_bases
GGCGCGCTATTTTCAATGAATATTTGAAGCAAACGTGCTTTTCTGGTATAATAGCTGAGGTTATTATATTAAAATCAAAT
TTTGGAGGGTTCCATAAAAA

Downstream 100 bases:

>100_bases
TAAAAAAATTACTGACAGAGTTGTCAGTAATTTTTTTATTTGACCATAAGTGCAATGACTAGTCCAAAAAGATTCCAAAG
AAAATGGACACTCATATTGA

Product: putative glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: Aquaglyceroporin; Glyceroaquaporin [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MDVTWTVKYITEFVGTALLIIMGNGAVANVELKGTKAHAQSWMIIGWGYGLGVMLPAVAFGNITSQINPAFTLGLAASGL
FPWAHVAQYIIAQVLGAMFGQLLIVMVYRPYYLKTQNPNAILGTFSTIDNVDDNSEKTRLGATINGFLNEFLGSFVLFFG
AVAATNIFFGSQSITWMTNYLKGQGADVSSSDVMNQIWVQASGASASKMIAHLFLGFLVMGLVVALGGPTGPGLNPARDF
GPRLVHSLLPKSVLGEAKGSSKWWYAWVPVLAPILASLAAVALFKMIYL

Sequences:

>Translated_289_residues
MDVTWTVKYITEFVGTALLIIMGNGAVANVELKGTKAHAQSWMIIGWGYGLGVMLPAVAFGNITSQINPAFTLGLAASGL
FPWAHVAQYIIAQVLGAMFGQLLIVMVYRPYYLKTQNPNAILGTFSTIDNVDDNSEKTRLGATINGFLNEFLGSFVLFFG
AVAATNIFFGSQSITWMTNYLKGQGADVSSSDVMNQIWVQASGASASKMIAHLFLGFLVMGLVVALGGPTGPGLNPARDF
GPRLVHSLLPKSVLGEAKGSSKWWYAWVPVLAPILASLAAVALFKMIYL
>Mature_289_residues
MDVTWTVKYITEFVGTALLIIMGNGAVANVELKGTKAHAQSWMIIGWGYGLGVMLPAVAFGNITSQINPAFTLGLAASGL
FPWAHVAQYIIAQVLGAMFGQLLIVMVYRPYYLKTQNPNAILGTFSTIDNVDDNSEKTRLGATINGFLNEFLGSFVLFFG
AVAATNIFFGSQSITWMTNYLKGQGADVSSSDVMNQIWVQASGASASKMIAHLFLGFLVMGLVVALGGPTGPGLNPARDF
GPRLVHSLLPKSVLGEAKGSSKWWYAWVPVLAPILASLAAVALFKMIYL

Specific function: Mixed channel protein that transports both water and glycerol [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6321054, Length=248, Percent_Identity=29.4354838709677, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 30936; Mature: 30936

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVTWTVKYITEFVGTALLIIMGNGAVANVELKGTKAHAQSWMIIGWGYGLGVMLPAVAF
CCCEEHHHHHHHHHHHHEEEEECCCCEEEEEECCCHHHHCEEEEEEECCCHHHHHHHHHH
GNITSQINPAFTLGLAASGLFPWAHVAQYIIAQVLGAMFGQLLIVMVYRPYYLKTQNPNA
CCHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCE
ILGTFSTIDNVDDNSEKTRLGATINGFLNEFLGSFVLFFGAVAATNIFFGSQSITWMTNY
EEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LKGQGADVSSSDVMNQIWVQASGASASKMIAHLFLGFLVMGLVVALGGPTGPGLNPARDF
HCCCCCCCCHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
GPRLVHSLLPKSVLGEAKGSSKWWYAWVPVLAPILASLAAVALFKMIYL
HHHHHHHHCCHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MDVTWTVKYITEFVGTALLIIMGNGAVANVELKGTKAHAQSWMIIGWGYGLGVMLPAVAF
CCCEEHHHHHHHHHHHHEEEEECCCCEEEEEECCCHHHHCEEEEEEECCCHHHHHHHHHH
GNITSQINPAFTLGLAASGLFPWAHVAQYIIAQVLGAMFGQLLIVMVYRPYYLKTQNPNA
CCHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCE
ILGTFSTIDNVDDNSEKTRLGATINGFLNEFLGSFVLFFGAVAATNIFFGSQSITWMTNY
EEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LKGQGADVSSSDVMNQIWVQASGASASKMIAHLFLGFLVMGLVVALGGPTGPGLNPARDF
HCCCCCCCCHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
GPRLVHSLLPKSVLGEAKGSSKWWYAWVPVLAPILASLAAVALFKMIYL
HHHHHHHHCCHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]