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The map label for this gene is ksgA [H]

Identifier: 125624667

GI number: 125624667

Start: 1864629

End: 1865513

Strand: Reverse

Name: ksgA [H]

Synonym: llmg_1882

Alternate gene names: 125624667

Gene position: 1865513-1864629 (Counterclockwise)

Preceding gene: 125624668

Following gene: 125624666

Centisome position: 73.75

GC content: 36.95

Gene sequence:

>885_bases
ATGACAGAAAATAATATAGACCGCATCTCCAATATCATTCGTACTCAAGACATTCTACGTCGCCATGATTTTAATTTTAA
GAAAAAATTTGGTCAAAATTTCCTGACCGACCATAATATTTTGACCAAAATTACGGAGACGGCTGACTTGTCCAAAGAAG
TGAATGTCATCGAAATTGGACCCGGAATTGGTTCATTATCACAGTATCTTTTAGAAGAAGCCGCTGAGGTTATGGCTTTT
GAAATTGACAAATCTTTGATTCCAATTTTAGAAGAAACGATGGCGCCTTACGATAATTTTACTTTGGTATCGGCAGATAT
TTTGAAAGTTGATTTGCTTTCTGAAATTCAAAAATTTAAAAATCCAAATTTACCAATTAAAGTGGTTGCGAATTTGCCCT
ACTACATCACTACTCCGATTTTGATGCATTTGATTGAAAGCAAAATTCCTTTTTCTGAATTCGTTGTGATGATGCAAAAA
GAAGTGGCAGACCGAATTGCTGCAAGCCCTAAAACCAAGGCTTACGGCTCATTATCAATTGCCGTTCAATATTATATGGA
AGCAAGTGTCGCCTTTATCGTCCCACGAACAGTCTTTATTCCTGCCCCAAATGTCGATTCGGCTATTTTGAAAATGGTTC
GACGTGAAGAACCTTTAGTTGAAGTAGAAGATGAAGAATGGTTCTTTAAAACGATGCACAGCAGCTTTGTTCACCGTCGT
AAAACCTTGATGAATAACATGCAAGCAGCATTTGGAAAAGAAAGTAAACCAGAAATAGAAAAATTATTGGCACAAGCAGA
AATCTCGCCAACAATACGTGGAGAAGCTTTGTCCATTGAGGAATTCGCTAAATTGGCTGATGCGCTTTTACCTCTTAAAA
AGTAA

Upstream 100 bases:

>100_bases
GATTATTAAGGCTGAACCTTAAATTAGTCAATTATAGATTACTTTCATTTCTAGACAGATTTGTCAGTAAAAAATTAGAT
ATAAATAAAAGAAAGAAAAA

Downstream 100 bases:

>100_bases
ATAAAAATTAAAAGAAAACGTTAACTCATGTTAGCGTTTTTGTGTTATCATGGAAGAAAATGATGAGGTCATTCGATCAT
TTAATTTTATATTTAAAGGA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MTENNIDRISNIIRTQDILRRHDFNFKKKFGQNFLTDHNILTKITETADLSKEVNVIEIGPGIGSLSQYLLEEAAEVMAF
EIDKSLIPILEETMAPYDNFTLVSADILKVDLLSEIQKFKNPNLPIKVVANLPYYITTPILMHLIESKIPFSEFVVMMQK
EVADRIAASPKTKAYGSLSIAVQYYMEASVAFIVPRTVFIPAPNVDSAILKMVRREEPLVEVEDEEWFFKTMHSSFVHRR
KTLMNNMQAAFGKESKPEIEKLLAQAEISPTIRGEALSIEEFAKLADALLPLKK

Sequences:

>Translated_294_residues
MTENNIDRISNIIRTQDILRRHDFNFKKKFGQNFLTDHNILTKITETADLSKEVNVIEIGPGIGSLSQYLLEEAAEVMAF
EIDKSLIPILEETMAPYDNFTLVSADILKVDLLSEIQKFKNPNLPIKVVANLPYYITTPILMHLIESKIPFSEFVVMMQK
EVADRIAASPKTKAYGSLSIAVQYYMEASVAFIVPRTVFIPAPNVDSAILKMVRREEPLVEVEDEEWFFKTMHSSFVHRR
KTLMNNMQAAFGKESKPEIEKLLAQAEISPTIRGEALSIEEFAKLADALLPLKK
>Mature_293_residues
TENNIDRISNIIRTQDILRRHDFNFKKKFGQNFLTDHNILTKITETADLSKEVNVIEIGPGIGSLSQYLLEEAAEVMAFE
IDKSLIPILEETMAPYDNFTLVSADILKVDLLSEIQKFKNPNLPIKVVANLPYYITTPILMHLIESKIPFSEFVVMMQKE
VADRIAASPKTKAYGSLSIAVQYYMEASVAFIVPRTVFIPAPNVDSAILKMVRREEPLVEVEDEEWFFKTMHSSFVHRRK
TLMNNMQAAFGKESKPEIEKLLAQAEISPTIRGEALSIEEFAKLADALLPLKK

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=280, Percent_Identity=30, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI7657198, Length=223, Percent_Identity=30.0448430493274, Blast_Score=92, Evalue=6e-19,
Organism=Escherichia coli, GI1786236, Length=263, Percent_Identity=30.4182509505703, Blast_Score=142, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI25141369, Length=298, Percent_Identity=28.1879194630872, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI25146882, Length=224, Percent_Identity=33.4821428571429, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324989, Length=224, Percent_Identity=26.3392857142857, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21358017, Length=245, Percent_Identity=31.8367346938775, Blast_Score=104, Evalue=9e-23,
Organism=Drosophila melanogaster, GI21357273, Length=290, Percent_Identity=25.1724137931034, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 33394; Mature: 33263

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENNIDRISNIIRTQDILRRHDFNFKKKFGQNFLTDHNILTKITETADLSKEVNVIEIG
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHCCCCHHHHHHHHHHHHCCCCCEEEEC
PGIGSLSQYLLEEAAEVMAFEIDKSLIPILEETMAPYDNFTLVSADILKVDLLSEIQKFK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHC
NPNLPIKVVANLPYYITTPILMHLIESKIPFSEFVVMMQKEVADRIAASPKTKAYGSLSI
CCCCCEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
AVQYYMEASVAFIVPRTVFIPAPNVDSAILKMVRREEPLVEVEDEEWFFKTMHSSFVHRR
HHHHHHHHHHHEEECCEEEEECCCCCHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHH
KTLMNNMQAAFGKESKPEIEKLLAQAEISPTIRGEALSIEEFAKLADALLPLKK
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TENNIDRISNIIRTQDILRRHDFNFKKKFGQNFLTDHNILTKITETADLSKEVNVIEIG
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHCCCCHHHHHHHHHHHHCCCCCEEEEC
PGIGSLSQYLLEEAAEVMAFEIDKSLIPILEETMAPYDNFTLVSADILKVDLLSEIQKFK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHC
NPNLPIKVVANLPYYITTPILMHLIESKIPFSEFVVMMQKEVADRIAASPKTKAYGSLSI
CCCCCEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
AVQYYMEASVAFIVPRTVFIPAPNVDSAILKMVRREEPLVEVEDEEWFFKTMHSSFVHRR
HHHHHHHHHHHEEECCEEEEECCCCCHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHH
KTLMNNMQAAFGKESKPEIEKLLAQAEISPTIRGEALSIEEFAKLADALLPLKK
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]