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The map label for this gene is folD

Identifier: 125624490

GI number: 125624490

Start: 1667891

End: 1668748

Strand: Reverse

Name: folD

Synonym: llmg_1693

Alternate gene names: 125624490

Gene position: 1668748-1667891 (Counterclockwise)

Preceding gene: 125624492

Following gene: 125624489

Centisome position: 65.97

GC content: 40.56

Gene sequence:

>858_bases
ATGAACTTGATAGATGGAAAAGCACTTGCAGCTAAAATGCAAGCTGAGTTGAAAGTTAAAGTCGATAAGTTAAAAGAAGC
AGGCAGCGTTCCTGGACTTGCTGTTATCCTTGTGGGTGAAGACCCAGCAAGTCAAATCTATGTCCGAAATAAAGCCCGTC
AAGCAACGGCAATTGGTTTGAATTCTAGCGTAGTTCGTCTACCAGAAACAGTTTCAGAAAAAGAGCTTTTGGAGCTGATT
GAACAATATAATCAATCAGAACAGTGGCATGGTATTTTGGTTCAATTACCTTTACCAAAACATATTTCTGAAGAAAAAGT
TTTATTAGCAATTGACCCTGAAAAGGATGTAGATGGTTTTCACCCAATGAATATGGGGCGTTTGTGGGCTGGAAATCCGT
TGATGATTCCTTCAACACCAGCTGGAATTATGGAGATGTTCCGTGAATATAATGTCGAACTTTCGGGAAAACGTGCGGTG
GTGATTGGGCGTTCAAACATTGTTGGAAAACCAATGGCACAGTTACTGATGATGGCAGATGCGACGGTGACAATTGCTCA
TTCAAGAACAAAAAACTTACGAGAATTAACTAAAGAAGCGGATATATTAGTGGTAGCCATTGGTCGTGACCGGATGATTA
AAGCGGATGATGTTAAAGATGGAGCAGTTGTCATTGATGTCGGCATGAATCGTGATGAAGACGGAAAATTGCATGGCGAT
GTTGATTTTGATGAAGTCAAAGAAGTTGCAAGTTTGATTACTCCTGTTCCTGGTGGAGTTGGTCCAATGACCATTACAAT
GTTGATGGAACAAACGGTGCGTGCGGCAACAAGAAAAATGAATGAAAATAGTAACTGA

Upstream 100 bases:

>100_bases
TGAGAAAGCTGATGAAAAATTACGAACAATTAAAACTAAAGCTGACAAATCCAATTTGAGGAGTTGAGAGTTGAAAGTCG
GCTTATGGTATAATAGTTTT

Downstream 100 bases:

>100_bases
TAAGAATAGATGAAAGAGTAATTAAGTTTATAAGCAAAGTTCTCCTCAATTATTGATAATAAATAATTGGTCAGGACATA
AACTTTTTACTTTTTTTCGA

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MNLIDGKALAAKMQAELKVKVDKLKEAGSVPGLAVILVGEDPASQIYVRNKARQATAIGLNSSVVRLPETVSEKELLELI
EQYNQSEQWHGILVQLPLPKHISEEKVLLAIDPEKDVDGFHPMNMGRLWAGNPLMIPSTPAGIMEMFREYNVELSGKRAV
VIGRSNIVGKPMAQLLMMADATVTIAHSRTKNLRELTKEADILVVAIGRDRMIKADDVKDGAVVIDVGMNRDEDGKLHGD
VDFDEVKEVASLITPVPGGVGPMTITMLMEQTVRAATRKMNENSN

Sequences:

>Translated_285_residues
MNLIDGKALAAKMQAELKVKVDKLKEAGSVPGLAVILVGEDPASQIYVRNKARQATAIGLNSSVVRLPETVSEKELLELI
EQYNQSEQWHGILVQLPLPKHISEEKVLLAIDPEKDVDGFHPMNMGRLWAGNPLMIPSTPAGIMEMFREYNVELSGKRAV
VIGRSNIVGKPMAQLLMMADATVTIAHSRTKNLRELTKEADILVVAIGRDRMIKADDVKDGAVVIDVGMNRDEDGKLHGD
VDFDEVKEVASLITPVPGGVGPMTITMLMEQTVRAATRKMNENSN
>Mature_285_residues
MNLIDGKALAAKMQAELKVKVDKLKEAGSVPGLAVILVGEDPASQIYVRNKARQATAIGLNSSVVRLPETVSEKELLELI
EQYNQSEQWHGILVQLPLPKHISEEKVLLAIDPEKDVDGFHPMNMGRLWAGNPLMIPSTPAGIMEMFREYNVELSGKRAV
VIGRSNIVGKPMAQLLMMADATVTIAHSRTKNLRELTKEADILVVAIGRDRMIKADDVKDGAVVIDVGMNRDEDGKLHGD
VDFDEVKEVASLITPVPGGVGPMTITMLMEQTVRAATRKMNENSN

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=288, Percent_Identity=45.8333333333333, Blast_Score=242, Evalue=3e-64,
Organism=Homo sapiens, GI222418558, Length=292, Percent_Identity=45.5479452054795, Blast_Score=235, Evalue=3e-62,
Organism=Homo sapiens, GI94721354, Length=292, Percent_Identity=45.8904109589041, Blast_Score=228, Evalue=3e-60,
Organism=Homo sapiens, GI36796743, Length=199, Percent_Identity=28.1407035175879, Blast_Score=78, Evalue=8e-15,
Organism=Escherichia coli, GI1786741, Length=275, Percent_Identity=51.6363636363636, Blast_Score=275, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI17568735, Length=257, Percent_Identity=43.9688715953307, Blast_Score=204, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6319558, Length=293, Percent_Identity=45.0511945392491, Blast_Score=247, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6321643, Length=302, Percent_Identity=43.7086092715232, Blast_Score=239, Evalue=3e-64,
Organism=Saccharomyces cerevisiae, GI6322933, Length=313, Percent_Identity=25.5591054313099, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24645718, Length=287, Percent_Identity=47.3867595818815, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI17137370, Length=287, Percent_Identity=47.3867595818815, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI62472483, Length=287, Percent_Identity=47.3867595818815, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI45551871, Length=287, Percent_Identity=47.3867595818815, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI17136818, Length=300, Percent_Identity=45, Blast_Score=242, Evalue=2e-64,
Organism=Drosophila melanogaster, GI17136816, Length=300, Percent_Identity=45, Blast_Score=242, Evalue=2e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_LACLM (A2RLU5)

Other databases:

- EMBL:   AM406671
- RefSeq:   YP_001032973.1
- ProteinModelPortal:   A2RLU5
- SMR:   A2RLU5
- STRING:   A2RLU5
- GeneID:   4797860
- GenomeReviews:   AM406671_GR
- KEGG:   llm:llmg_1693
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   TYVRNKQ
- ProtClustDB:   PRK14179
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 31166; Mature: 31166

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLIDGKALAAKMQAELKVKVDKLKEAGSVPGLAVILVGEDPASQIYVRNKARQATAIGL
CCCCCCHHHHHHHHHHHEEEHHHHHHCCCCCCEEEEEECCCCCCEEEEECCCCCEEEECC
NSSVVRLPETVSEKELLELIEQYNQSEQWHGILVQLPLPKHISEEKVLLAIDPEKDVDGF
CCCEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCC
HPMNMGRLWAGNPLMIPSTPAGIMEMFREYNVELSGKRAVVIGRSNIVGKPMAQLLMMAD
CCCCCCCEECCCEEECCCCCHHHHHHHHHCCCEECCCEEEEEECCCCCCCHHHHHHHHHC
ATVTIAHSRTKNLRELTKEADILVVAIGRDRMIKADDVKDGAVVIDVGMNRDEDGKLHGD
CEEEEEEHHHHHHHHHHCCCCEEEEEECCCCEEECCCCCCCEEEEEECCCCCCCCCEECC
VDFDEVKEVASLITPVPGGVGPMTITMLMEQTVRAATRKMNENSN
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNLIDGKALAAKMQAELKVKVDKLKEAGSVPGLAVILVGEDPASQIYVRNKARQATAIGL
CCCCCCHHHHHHHHHHHEEEHHHHHHCCCCCCEEEEEECCCCCCEEEEECCCCCEEEECC
NSSVVRLPETVSEKELLELIEQYNQSEQWHGILVQLPLPKHISEEKVLLAIDPEKDVDGF
CCCEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCC
HPMNMGRLWAGNPLMIPSTPAGIMEMFREYNVELSGKRAVVIGRSNIVGKPMAQLLMMAD
CCCCCCCEECCCEEECCCCCHHHHHHHHHCCCEECCCEEEEEECCCCCCCHHHHHHHHHC
ATVTIAHSRTKNLRELTKEADILVVAIGRDRMIKADDVKDGAVVIDVGMNRDEDGKLHGD
CEEEEEEHHHHHHHHHHCCCCEEEEEECCCCEEECCCCCCCEEEEEECCCCCCCCCEECC
VDFDEVKEVASLITPVPGGVGPMTITMLMEQTVRAATRKMNENSN
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA