Click here to switch to the map view.

The map label for this gene is pbpX [H]

Identifier: 125624477

GI number: 125624477

Start: 1653310

End: 1655607

Strand: Reverse

Name: pbpX [H]

Synonym: llmg_1679

Alternate gene names: 125624477

Gene position: 1655607-1653310 (Counterclockwise)

Preceding gene: 125624478

Following gene: 125624476

Centisome position: 65.45

GC content: 38.25

Gene sequence:

>2298_bases
ATGATAAAATTTTTAAGAAAACTAATTTTTTTTCCTTTTAGAAAAGTATCACGAAATGCCAGAAAAACGAATTGGAGTCC
AGAAAGAAATCGTAAACGAGTAGGTAAGAGCCTGTTTTTCCTAGCTATTGCGCTGTTTACGGTTTTTATATTTCGTTTTG
TCTGGTTAATTACCGTCAACCATGTCGGTGATACCAATCTTAAAGAGATGGCTAAGAGTAATTATCAGAGTACGGTACCC
GTTCAAGCAAAGCGTGGAACGATTTATGATCGGACAGGAACACCCATTGCGGTCGACTCTAGTACTTATACAATTTATGC
CGTCATTGATAAAACGCAGGTTGATTCAAATGGAAACCCTCTCTATGTTAAGAAAGAGGATTTTGATAAAGTCGCTACAT
TTTTAAGTAGTAAACTTAAGATTGACCGTAGCTTAATTGAAAAACAACTTAATTCTAAGTTAAAACAAGTTCAATTTGGT
GCAGAAGGAAGTAATATTTCCTTGCAACAGATGCAAGAGATTCAAAAGGCAGCAGAGAATGAAAAAATAGTTGGGCTTGG
TTTTACGTCTAATGTGTCTCGTTCTTATCCATTTGGAAATTTTGCTTCTCAATTTATTGGAATTGCTCGTCCTAAAGATG
AAAATGGTTTTCAAACCTTAAAAGGGGACATGGGCTTAGAAAAAGCCTTCGATAACGTTTTGAGTGGTGAAAATGGTCAA
GAAACATACCAAAAAGATATTTACGGTCGTCCGATTCCGGGAACAACGAAAGTAGATAAAGCTGTTAAAAATGGTCAAGA
TGTTTATACAACTCTTGATGCTCAACTTCAAAGAAACCTTGAAGGTTATATGGACAAAGCCGCTACGGATACTGGAGCTC
AACAGCTTTCAGGAACCTTGGTTGATGCCCATACCGGTGAGATTTTAGCCACAAGTCAACGTCCAACTTATACGGCAACC
ACAATCAATAGTGCAGAAAAACAAAAATATTTCACATGGAATAGTTTGCTTTCACAATCAGCTTTTGAACCGGGTTCAAC
ATTTAAGACTTTCTTAATGGCCGGCGCATTAGATAGTGGTAAAGTTAATCTTAACGAAACTTATCAACGGAAACTTCAAG
TTTATGATACAACAATTAATGACTGGGACGTCACGGAAAATAAAAGTTATACTTTGCCAGAAACCGTAACTTATGCTCAA
GGTTTTGCTTTATCTTCAAATATTGGGATGAGTAAGATTGAAATGAATATGGGTGATGCTTTATGGGGAAGTTACCTTAA
TAAATTTAAATTCGGTCTAAAAGTACGGGCTGGTTTAGATGGTGAAAATCCAGGGGCGCTTCCTTCATCAAATGCAGTTT
CACAAATTCAATCTTCATTTGGTCAAGGGGTTGCAGTCACGCCGCTTCAATTGATAAGAGGCTGGACTGCCATTGCTGGA
AACGGAACAATGCTTGAACCGCATATTGTCAGCAAAGTAGTCGATACGAACACCAAAACAAGCTTAACTTCTAAAGCAGA
AGTAGTTGGACACCCTGTTTCCAATGAAGCTGCAAGTGGGGTACGAGACTTGATGCTCACTGTAAATACTGACCCAGTTT
ACGGAACTTCTTATTCAACAGCAGGAGATCCTGAGCAAGATCTTGCGGCGGGACCATTGTTTATGGTAAATGGAGAACCA
GCTGCAGTGAAAACAGGGACTGCCCAAATCGCAGCAACTACTGGTGGATATATGACTGGTTCTCAGGATTATCTTTATTC
AGCTGTAGTGATGTATCCTGCTAAGAATCCTGATTTTATCTTTTATATGAATGTCAAAATTCCTTCAGAACCCTGGACGC
TTAAATATATTGCTCGGGTAGCAAATCCTTTGTTAACAAGTGCAGAAGCAATGAAGGGTGATTTAACGGCTACTTCTAGT
ACTGATTCTGATATTAAAGCAGGAAAAGTGACGATTGAGAATTACAAAGGTAAGGATTCAGGTGATACGGCGGATAACTT
GCGTCGTACGGTCATCAGTCCAGTTATCATTGGAACAGGTGCTAAAGTAACAGCTCAATCAATTGCTGAGGGTGAAAAGG
TTGCGGCGAATACACGAATTTTACTTTTAACGAACGATAAAGATCAGGTGATGCCTGATATGTATGATTGGTCTAAAAAA
GAAGTTGAACAGTTAGCAAATTGGTTTGGAATTAAAGTGACTTACGAAGGTTCAGGAAATAAAGTCTTGACCCAAAGTAT
TGAAACTTCAACTAATGTCAAAAAAGGTCAAACGCTCACTGTAAAAATGGGTAATTAA

Upstream 100 bases:

>100_bases
ACCAACAAATTTTAGATCTAACATCTGGTAATAGAGTGTTAGATGCAGCAAACAAAGCAAGTTTAAAAGCAAATCCATCT
AATGTATTGAAAGCAACGAA

Downstream 100 bases:

>100_bases
GTAGAATTTAAATTTATCAATTTGGGGCTCCACAAAGTTGGTCTTTGTGGAGCTTCAAAAATTAGGAGAAGAAAATGTTA
TTGAATGGAATAGTGGCGGC

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-2x; PBP2x [H]

Number of amino acids: Translated: 765; Mature: 765

Protein sequence:

>765_residues
MIKFLRKLIFFPFRKVSRNARKTNWSPERNRKRVGKSLFFLAIALFTVFIFRFVWLITVNHVGDTNLKEMAKSNYQSTVP
VQAKRGTIYDRTGTPIAVDSSTYTIYAVIDKTQVDSNGNPLYVKKEDFDKVATFLSSKLKIDRSLIEKQLNSKLKQVQFG
AEGSNISLQQMQEIQKAAENEKIVGLGFTSNVSRSYPFGNFASQFIGIARPKDENGFQTLKGDMGLEKAFDNVLSGENGQ
ETYQKDIYGRPIPGTTKVDKAVKNGQDVYTTLDAQLQRNLEGYMDKAATDTGAQQLSGTLVDAHTGEILATSQRPTYTAT
TINSAEKQKYFTWNSLLSQSAFEPGSTFKTFLMAGALDSGKVNLNETYQRKLQVYDTTINDWDVTENKSYTLPETVTYAQ
GFALSSNIGMSKIEMNMGDALWGSYLNKFKFGLKVRAGLDGENPGALPSSNAVSQIQSSFGQGVAVTPLQLIRGWTAIAG
NGTMLEPHIVSKVVDTNTKTSLTSKAEVVGHPVSNEAASGVRDLMLTVNTDPVYGTSYSTAGDPEQDLAAGPLFMVNGEP
AAVKTGTAQIAATTGGYMTGSQDYLYSAVVMYPAKNPDFIFYMNVKIPSEPWTLKYIARVANPLLTSAEAMKGDLTATSS
TDSDIKAGKVTIENYKGKDSGDTADNLRRTVISPVIIGTGAKVTAQSIAEGEKVAANTRILLLTNDKDQVMPDMYDWSKK
EVEQLANWFGIKVTYEGSGNKVLTQSIETSTNVKKGQTLTVKMGN

Sequences:

>Translated_765_residues
MIKFLRKLIFFPFRKVSRNARKTNWSPERNRKRVGKSLFFLAIALFTVFIFRFVWLITVNHVGDTNLKEMAKSNYQSTVP
VQAKRGTIYDRTGTPIAVDSSTYTIYAVIDKTQVDSNGNPLYVKKEDFDKVATFLSSKLKIDRSLIEKQLNSKLKQVQFG
AEGSNISLQQMQEIQKAAENEKIVGLGFTSNVSRSYPFGNFASQFIGIARPKDENGFQTLKGDMGLEKAFDNVLSGENGQ
ETYQKDIYGRPIPGTTKVDKAVKNGQDVYTTLDAQLQRNLEGYMDKAATDTGAQQLSGTLVDAHTGEILATSQRPTYTAT
TINSAEKQKYFTWNSLLSQSAFEPGSTFKTFLMAGALDSGKVNLNETYQRKLQVYDTTINDWDVTENKSYTLPETVTYAQ
GFALSSNIGMSKIEMNMGDALWGSYLNKFKFGLKVRAGLDGENPGALPSSNAVSQIQSSFGQGVAVTPLQLIRGWTAIAG
NGTMLEPHIVSKVVDTNTKTSLTSKAEVVGHPVSNEAASGVRDLMLTVNTDPVYGTSYSTAGDPEQDLAAGPLFMVNGEP
AAVKTGTAQIAATTGGYMTGSQDYLYSAVVMYPAKNPDFIFYMNVKIPSEPWTLKYIARVANPLLTSAEAMKGDLTATSS
TDSDIKAGKVTIENYKGKDSGDTADNLRRTVISPVIIGTGAKVTAQSIAEGEKVAANTRILLLTNDKDQVMPDMYDWSKK
EVEQLANWFGIKVTYEGSGNKVLTQSIETSTNVKKGQTLTVKMGN
>Mature_765_residues
MIKFLRKLIFFPFRKVSRNARKTNWSPERNRKRVGKSLFFLAIALFTVFIFRFVWLITVNHVGDTNLKEMAKSNYQSTVP
VQAKRGTIYDRTGTPIAVDSSTYTIYAVIDKTQVDSNGNPLYVKKEDFDKVATFLSSKLKIDRSLIEKQLNSKLKQVQFG
AEGSNISLQQMQEIQKAAENEKIVGLGFTSNVSRSYPFGNFASQFIGIARPKDENGFQTLKGDMGLEKAFDNVLSGENGQ
ETYQKDIYGRPIPGTTKVDKAVKNGQDVYTTLDAQLQRNLEGYMDKAATDTGAQQLSGTLVDAHTGEILATSQRPTYTAT
TINSAEKQKYFTWNSLLSQSAFEPGSTFKTFLMAGALDSGKVNLNETYQRKLQVYDTTINDWDVTENKSYTLPETVTYAQ
GFALSSNIGMSKIEMNMGDALWGSYLNKFKFGLKVRAGLDGENPGALPSSNAVSQIQSSFGQGVAVTPLQLIRGWTAIAG
NGTMLEPHIVSKVVDTNTKTSLTSKAEVVGHPVSNEAASGVRDLMLTVNTDPVYGTSYSTAGDPEQDLAAGPLFMVNGEP
AAVKTGTAQIAATTGGYMTGSQDYLYSAVVMYPAKNPDFIFYMNVKIPSEPWTLKYIARVANPLLTSAEAMKGDLTATSS
TDSDIKAGKVTIENYKGKDSGDTADNLRRTVISPVIIGTGAKVTAQSIAEGEKVAANTRILLLTNDKDQVMPDMYDWSKK
EVEQLANWFGIKVTYEGSGNKVLTQSIETSTNVKKGQTLTVKMGN

Specific function: Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. Beta-lactams inactivate the PBPs by acylating an essential serine residue in the active site of these proteins [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PASTA domains [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=578, Percent_Identity=26.643598615917, Blast_Score=131, Evalue=2e-31,
Organism=Escherichia coli, GI1786854, Length=572, Percent_Identity=20.8041958041958, Blast_Score=99, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005543
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03793 PASTA; PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 83714; Mature: 83714

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKFLRKLIFFPFRKVSRNARKTNWSPERNRKRVGKSLFFLAIALFTVFIFRFVWLITVN
CHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
HVGDTNLKEMAKSNYQSTVPVQAKRGTIYDRTGTPIAVDSSTYTIYAVIDKTQVDSNGNP
CCCCCCHHHHHHCCCCCCCCEEECCCEEEECCCCEEEECCCCEEEEEEEEEEEECCCCCE
LYVKKEDFDKVATFLSSKLKIDRSLIEKQLNSKLKQVQFGAEGSNISLQQMQEIQKAAEN
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHCC
EKIVGLGFTSNVSRSYPFGNFASQFIGIARPKDENGFQTLKGDMGLEKAFDNVLSGENGQ
CEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHCCCCHHHHHHHHHCCCCCH
ETYQKDIYGRPIPGTTKVDKAVKNGQDVYTTLDAQLQRNLEGYMDKAATDTGAQQLSGTL
HHHHHHCCCCCCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCHHHHCCEE
VDAHTGEILATSQRPTYTATTINSAEKQKYFTWNSLLSQSAFEPGSTFKTFLMAGALDSG
EECCCCCEEEECCCCCEEEEECCCHHHHHEEHHHHHHHHHCCCCCCHHHHHHHHCCCCCC
KVNLNETYQRKLQVYDTTINDWDVTENKSYTLPETVTYAQGFALSSNIGMSKIEMNMGDA
CCCCCHHHHHEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCEEEECCCCCEEEEECCCHH
LWGSYLNKFKFGLKVRAGLDGENPGALPSSNAVSQIQSSFGQGVAVTPLQLIRGWTAIAG
HHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEECHHHHHCCCEEEEC
NGTMLEPHIVSKVVDTNTKTSLTSKAEVVGHPVSNEAASGVRDLMLTVNTDPVYGTSYST
CCCEECHHHHHHHHCCCCCCCCCCCHHHHCCCCCCHHHCCCEEEEEEEECCCCCCCCCCC
AGDPEQDLAAGPLFMVNGEPAAVKTGTAQIAATTGGYMTGSQDYLYSAVVMYPAKNPDFI
CCCCHHHHCCCCEEEECCCCCEEECCCEEEEEECCCCCCCCHHHEEEEEEEECCCCCCEE
FYMNVKIPSEPWTLKYIARVANPLLTSAEAMKGDLTATSSTDSDIKAGKVTIENYKGKDS
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCEEEEECCCCCCC
GDTADNLRRTVISPVIIGTGAKVTAQSIAEGEKVAANTRILLLTNDKDQVMPDMYDWSKK
CCCHHHHHHHHHCCEEECCCCCHHHHHHHCCCCEECCEEEEEEECCCHHHCCCHHCCCHH
EVEQLANWFGIKVTYEGSGNKVLTQSIETSTNVKKGQTLTVKMGN
HHHHHHHHEEEEEEEECCCCEEEEEHHHCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MIKFLRKLIFFPFRKVSRNARKTNWSPERNRKRVGKSLFFLAIALFTVFIFRFVWLITVN
CHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
HVGDTNLKEMAKSNYQSTVPVQAKRGTIYDRTGTPIAVDSSTYTIYAVIDKTQVDSNGNP
CCCCCCHHHHHHCCCCCCCCEEECCCEEEECCCCEEEECCCCEEEEEEEEEEEECCCCCE
LYVKKEDFDKVATFLSSKLKIDRSLIEKQLNSKLKQVQFGAEGSNISLQQMQEIQKAAEN
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHCC
EKIVGLGFTSNVSRSYPFGNFASQFIGIARPKDENGFQTLKGDMGLEKAFDNVLSGENGQ
CEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHCCCCHHHHHHHHHCCCCCH
ETYQKDIYGRPIPGTTKVDKAVKNGQDVYTTLDAQLQRNLEGYMDKAATDTGAQQLSGTL
HHHHHHCCCCCCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCHHHHCCEE
VDAHTGEILATSQRPTYTATTINSAEKQKYFTWNSLLSQSAFEPGSTFKTFLMAGALDSG
EECCCCCEEEECCCCCEEEEECCCHHHHHEEHHHHHHHHHCCCCCCHHHHHHHHCCCCCC
KVNLNETYQRKLQVYDTTINDWDVTENKSYTLPETVTYAQGFALSSNIGMSKIEMNMGDA
CCCCCHHHHHEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCEEEECCCCCEEEEECCCHH
LWGSYLNKFKFGLKVRAGLDGENPGALPSSNAVSQIQSSFGQGVAVTPLQLIRGWTAIAG
HHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEECHHHHHCCCEEEEC
NGTMLEPHIVSKVVDTNTKTSLTSKAEVVGHPVSNEAASGVRDLMLTVNTDPVYGTSYST
CCCEECHHHHHHHHCCCCCCCCCCCHHHHCCCCCCHHHCCCEEEEEEEECCCCCCCCCCC
AGDPEQDLAAGPLFMVNGEPAAVKTGTAQIAATTGGYMTGSQDYLYSAVVMYPAKNPDFI
CCCCHHHHCCCCEEEECCCCCEEECCCEEEEEECCCCCCCCHHHEEEEEEEECCCCCCEE
FYMNVKIPSEPWTLKYIARVANPLLTSAEAMKGDLTATSSTDSDIKAGKVTIENYKGKDS
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCEEEEECCCCCCC
GDTADNLRRTVISPVIIGTGAKVTAQSIAEGEKVAANTRILLLTNDKDQVMPDMYDWSKK
CCCHHHHHHHHHCCEEECCCCCHHHHHHHCCCCEECCEEEEEEECCCHHHCCCHHCCCHH
EVEQLANWFGIKVTYEGSGNKVLTQSIETSTNVKKGQTLTVKMGN
HHHHHHHHEEEEEEEECCCCEEEEEHHHCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11463916; 8605631 [H]