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The map label for this gene is gadB [H]

Identifier: 125624009

GI number: 125624009

Start: 1147076

End: 1148476

Strand: Direct

Name: gadB [H]

Synonym: llmg_1179

Alternate gene names: 125624009

Gene position: 1147076-1148476 (Clockwise)

Preceding gene: 125624008

Following gene: 125624011

Centisome position: 45.35

GC content: 35.4

Gene sequence:

>1401_bases
ATGTTATACGGAAAAGAAAATCGAGATGAAGCGGAGTTCTTGGAACCAATTTTTGGTTCAGAAAGTGAACAAGTGGATTT
ACCTAAATATAAATTAGCTCAACAATCAATTGAGCCTCGAGTGGCCTATCAGTTAGTTCAAGATGAAATGCTAGATGAAG
GGAACGCTCGTTTAAATTTGGCCACATTCTGTCAAACTTATATGGAACCTGAAGCAGTCAAGCTGATGAGTCAGACCTTG
GAAAAAAATGCGATTGACAAATCAGAATATCCAAGAACAACTGAAATTGAAAACCGTTGCGTCAACATGATCGCTGACCT
TTGGAATGCGAGTGAAAAAGAAAAATTTATGGGGACTTCGACAATTGGTTCTTCAGAAGCTTGTATGCTTGGGGGAATGG
CTATGAAGTTTTCTTGGCGTAAGCGAGCAGAAAAATTAGGCCTAGATATTAATGCGAAAAAGCCAAACTTAGTCATTTCC
TCTGGTTATCAAGTTTGCTGGGAAAAATTCTGTGTTTATTGGGATATTGAAATGAGAGAAGTGCCAATGGATAGAGAACA
TATGTCAATCAATTTGGAAAAAGTGATGGATTATGTTGATGAATATACGATTGGAGTAGTTGGAATTATGGGGATTACTT
ATACTGGTCGTTATGATGATATCAAAGCTTTGGATAATTTGATTGAAGAATATAATAAACAGACAGACTACAAAGTTTAT
ATTCACGTAGATGCTGCTTCAGGAGGACTTTATGCTCCTTTTGTTGAGCCAGAACTTGAGTGGGATTTCCGTTTGAAAAA
TGTCATTTCAATCAATACTTCAGGACATAAATATGGTTTAGTATATCCTGGTGTAGGTTGGGTCTTGTGGCGTGACAAAA
AATATTTACCTGAAGAGTTAATTTTTAAAGTAAGTTATCTTGGAGGAGAATTACCAACAATGGCGATTAATTTTTCTCAC
AGTGCTTCTCAATTAATCGGTCAATACTATAATTTTGTACGTTATGGATTTGATGGATATAAAGCTATTCATGAGAGAAC
GCATAAAGTAGCCATGTATTTAGCAGAAGAAATTGAAAAAACAGGAATGTTTGAGATTATGAACGATGGGGCACAATTAC
CAATTGTCTGCTACAAATTAAAAGAAAATTCAAACCGTGGTTGGAATCTTTATGATTTGGCAGATCGTTTATTAATGAAG
GGATGGCAAGTGCCTGCTTATCCACTTCCTAAAAATTTGGAAAATGAAATCATTCAACGTTTAGTAATTCGAGCAGATTT
CGGGATGAATATGGCATTTAACTATGTTCAAGATATGCAAGAAGCAATTGATGCACTAAATAAGGCTCATATTCTATTTC
ATCAGGAACCTGAAAATAAAACATATGGCTTTACTCACTAA

Upstream 100 bases:

>100_bases
GATGTGAACCCTGCGATTTATCCAGCAGCTCGTGGAGAGCATCATATTATTAAAAAAGAAGAACATATCTTAAAACATTG
AAAAATTGGAGGATGTACAT

Downstream 100 bases:

>100_bases
AGATAAAAGCGATATATCTAAGATATATCGCTTTTATTTTGTTTTAGGCTATTTACTAATTAGCTTGTCGCTTATTATTT
TTCATAGTATTTATCCAAAA

Product: glutamate decarboxylase

Products: NA

Alternate protein names: GAD [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MLYGKENRDEAEFLEPIFGSESEQVDLPKYKLAQQSIEPRVAYQLVQDEMLDEGNARLNLATFCQTYMEPEAVKLMSQTL
EKNAIDKSEYPRTTEIENRCVNMIADLWNASEKEKFMGTSTIGSSEACMLGGMAMKFSWRKRAEKLGLDINAKKPNLVIS
SGYQVCWEKFCVYWDIEMREVPMDREHMSINLEKVMDYVDEYTIGVVGIMGITYTGRYDDIKALDNLIEEYNKQTDYKVY
IHVDAASGGLYAPFVEPELEWDFRLKNVISINTSGHKYGLVYPGVGWVLWRDKKYLPEELIFKVSYLGGELPTMAINFSH
SASQLIGQYYNFVRYGFDGYKAIHERTHKVAMYLAEEIEKTGMFEIMNDGAQLPIVCYKLKENSNRGWNLYDLADRLLMK
GWQVPAYPLPKNLENEIIQRLVIRADFGMNMAFNYVQDMQEAIDALNKAHILFHQEPENKTYGFTH

Sequences:

>Translated_466_residues
MLYGKENRDEAEFLEPIFGSESEQVDLPKYKLAQQSIEPRVAYQLVQDEMLDEGNARLNLATFCQTYMEPEAVKLMSQTL
EKNAIDKSEYPRTTEIENRCVNMIADLWNASEKEKFMGTSTIGSSEACMLGGMAMKFSWRKRAEKLGLDINAKKPNLVIS
SGYQVCWEKFCVYWDIEMREVPMDREHMSINLEKVMDYVDEYTIGVVGIMGITYTGRYDDIKALDNLIEEYNKQTDYKVY
IHVDAASGGLYAPFVEPELEWDFRLKNVISINTSGHKYGLVYPGVGWVLWRDKKYLPEELIFKVSYLGGELPTMAINFSH
SASQLIGQYYNFVRYGFDGYKAIHERTHKVAMYLAEEIEKTGMFEIMNDGAQLPIVCYKLKENSNRGWNLYDLADRLLMK
GWQVPAYPLPKNLENEIIQRLVIRADFGMNMAFNYVQDMQEAIDALNKAHILFHQEPENKTYGFTH
>Mature_466_residues
MLYGKENRDEAEFLEPIFGSESEQVDLPKYKLAQQSIEPRVAYQLVQDEMLDEGNARLNLATFCQTYMEPEAVKLMSQTL
EKNAIDKSEYPRTTEIENRCVNMIADLWNASEKEKFMGTSTIGSSEACMLGGMAMKFSWRKRAEKLGLDINAKKPNLVIS
SGYQVCWEKFCVYWDIEMREVPMDREHMSINLEKVMDYVDEYTIGVVGIMGITYTGRYDDIKALDNLIEEYNKQTDYKVY
IHVDAASGGLYAPFVEPELEWDFRLKNVISINTSGHKYGLVYPGVGWVLWRDKKYLPEELIFKVSYLGGELPTMAINFSH
SASQLIGQYYNFVRYGFDGYKAIHERTHKVAMYLAEEIEKTGMFEIMNDGAQLPIVCYKLKENSNRGWNLYDLADRLLMK
GWQVPAYPLPKNLENEIIQRLVIRADFGMNMAFNYVQDMQEAIDALNKAHILFHQEPENKTYGFTH

Specific function: Converts internalized glutamate to GABA and increases the internal pH. Involved in glutamate-dependent acid resistance [H]

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=387, Percent_Identity=23.2558139534884, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1787769, Length=442, Percent_Identity=45.0226244343891, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI1789934, Length=443, Percent_Identity=44.6952595936795, Blast_Score=409, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17557272, Length=366, Percent_Identity=24.5901639344262, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17543922, Length=387, Percent_Identity=24.5478036175711, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI25148342, Length=320, Percent_Identity=21.875, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323905, Length=466, Percent_Identity=37.5536480686695, Blast_Score=296, Evalue=5e-81,
Organism=Saccharomyces cerevisiae, GI6320500, Length=341, Percent_Identity=24.0469208211144, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI21355963, Length=401, Percent_Identity=24.9376558603491, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24654344, Length=401, Percent_Identity=24.9376558603491, Blast_Score=108, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010107
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.1.15 [H]

Molecular weight: Translated: 53924; Mature: 53924

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYGKENRDEAEFLEPIFGSESEQVDLPKYKLAQQSIEPRVAYQLVQDEMLDEGNARLNL
CCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEH
ATFCQTYMEPEAVKLMSQTLEKNAIDKSEYPRTTEIENRCVNMIADLWNASEKEKFMGTS
HHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCC
TIGSSEACMLGGMAMKFSWRKRAEKLGLDINAKKPNLVISSGYQVCWEKFCVYWDIEMRE
CCCCCCHHHHCCCCEEHHHHHHHHHHCCCEECCCCCEEEECCHHHHHHHEEEEEEEEHHC
VPMDREHMSINLEKVMDYVDEYTIGVVGIMGITYTGRYDDIKALDNLIEEYNKQTDYKVY
CCCCHHHHEEEHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEE
IHVDAASGGLYAPFVEPELEWDFRLKNVISINTSGHKYGLVYPGVGWVLWRDKKYLPEEL
EEEEECCCCEECCCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCEEEECCCCCCCHHH
IFKVSYLGGELPTMAINFSHSASQLIGQYYNFVRYGFDGYKAIHERTHKVAMYLAEEIEK
HHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
TGMFEIMNDGAQLPIVCYKLKENSNRGWNLYDLADRLLMKGWQVPAYPLPKNLENEIIQR
CCCHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
LVIRADFGMNMAFNYVQDMQEAIDALNKAHILFHQEPENKTYGFTH
HHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MLYGKENRDEAEFLEPIFGSESEQVDLPKYKLAQQSIEPRVAYQLVQDEMLDEGNARLNL
CCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEH
ATFCQTYMEPEAVKLMSQTLEKNAIDKSEYPRTTEIENRCVNMIADLWNASEKEKFMGTS
HHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCC
TIGSSEACMLGGMAMKFSWRKRAEKLGLDINAKKPNLVISSGYQVCWEKFCVYWDIEMRE
CCCCCCHHHHCCCCEEHHHHHHHHHHCCCEECCCCCEEEECCHHHHHHHEEEEEEEEHHC
VPMDREHMSINLEKVMDYVDEYTIGVVGIMGITYTGRYDDIKALDNLIEEYNKQTDYKVY
CCCCHHHHEEEHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEE
IHVDAASGGLYAPFVEPELEWDFRLKNVISINTSGHKYGLVYPGVGWVLWRDKKYLPEEL
EEEEECCCCEECCCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCEEEECCCCCCCHHH
IFKVSYLGGELPTMAINFSHSASQLIGQYYNFVRYGFDGYKAIHERTHKVAMYLAEEIEK
HHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
TGMFEIMNDGAQLPIVCYKLKENSNRGWNLYDLADRLLMKGWQVPAYPLPKNLENEIIQR
CCCHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
LVIRADFGMNMAFNYVQDMQEAIDALNKAHILFHQEPENKTYGFTH
HHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10411264; 11337471 [H]