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The map label for this gene is gadC [H]

Identifier: 125624008

GI number: 125624008

Start: 1145542

End: 1147056

Strand: Direct

Name: gadC [H]

Synonym: llmg_1178

Alternate gene names: 125624008

Gene position: 1145542-1147056 (Clockwise)

Preceding gene: 125624007

Following gene: 125624009

Centisome position: 45.29

GC content: 35.31

Gene sequence:

>1515_bases
ATGATGAATCAAAAAAAATTATCATTATTCGGTTTTTTCGCATTAACCGCTTCAATGGTTTTGACTGTCTATGAGTATCC
GACTTTTGCCACGTCAAAATTACATTTGGTGTTCTTTTTACTTCTCGGAGGACTACTATGGTTTTTGCCTGTAGCGCTCT
GCGCAGCAGAAATGGCGACGGTTGAAGGCTGGAAAAATGGTGGAATCTTTAGTTGGGTCAGTCAAACTTTAGGTGAGCGC
TTTGGTTTTGCAGCCATATTTTTTCAGTGGTTCCAAATTACAGTAGGTTTTGTCACTATGATCTATTTCATTTTAGGGGC
CCTCTCTTATGTGTTAAATTTTCAGGCGCTCAATACAGATCCATTGATAAAATTTATTGGTTTACTAATCATTTTTTGGG
GATTGACTTTTTCTCAATTAGGTGGGACGCAACGGACTGCCAAATTAGTAAAAGCTGGCTTTGTAGTTGGAATAGTGATT
CCATCGGTTATCTTGTTTGGATTAGCAGCGGCATACTTTATCGGAGGCAATCCTATAGAAATACCAATTAACAGCCATGC
TTTTGTACCAGATTTTTCACAGGTATCAACTTTAGTAGTTTTTGTTTCTTTTATTCTGGCTTATATGGGGGTAGAAGCCT
CAGCTTCACATATTAATGAACTTGAAAATCCAAAACGAAATTATCCCTTAGCAATGATTTTATTAGTAATTTTGGCTATT
TCTTTAGATGCCATAGGTGGATTTTCTGTAGCAGCAGTTATTCCTCAAAAAGAGTTATCATTAAGTGCAGGGGTAATCCA
AACTTTTCAAACGTTAATCTTACATTTTAATCATCATTTGGGATGGTTAGTTAAAGTGATTGCACTAATGATTGCCTTTG
GGGTTATGGGAGAAGTGAGTTCATGGGTTGTTGGTCCTTCTAGAGGGATGTTTGCAGCAGCACAAAGAGGTTTATTACCA
AAATTTTTACGTAAAACGAATACACATGAAGTCCCTGTTCCTTTAGTTATGATTCAAGGAATCATTGTTACACTTTGGGG
CGCTGTATTAACTTTTGGAGGAGGAGGAAATAATTTATCTTTCTTAGTTGCCATTTCACTGACTGTAGTGATTTATTTGG
TGGGTTACCTCTTGTTCTTTATTGTTTACTTTGTTTTAATCTATAAAAAACAAAATTTAAAGCGTACTTATAATGTTCCA
GGTAAAATAATAGGAAAAACAATCATTGCAGGAATTGGATTCTTATTATCAATTTTTGCTCTATTTATTTCCTTTGTTCC
TCCAGCATCAATTGCGAAAAATGAAACTCACACCTATCAAATGATACTTCTTATAAGTTTTGTTGTGACCGCTATCTTGC
CATTTATTATTTATGAATTGCATGATAAAAAGGGACATGATACTATTGAAGAACCAACACACTTTAAAGCAGGAGATGTG
AACCCTGCGATTTATCCAGCAGCTCGTGGAGAGCATCATATTATTAAAAAAGAAGAACATATCTTAAAACATTGA

Upstream 100 bases:

>100_bases
GATTTTCTTTATTCTAGAGCATATACTAGAGGGTGAAGATAGGTTGTCTGAAGCATTATAACTTGTCTTTTAAAAAATTC
AATCATAAATATAAGGAGGT

Downstream 100 bases:

>100_bases
AAAATTGGAGGATGTACATATGTTATACGGAAAAGAAAATCGAGATGAAGCGGAGTTCTTGGAACCAATTTTTGGTTCAG
AAAGTGAACAAGTGGATTTA

Product: putative glutamate/gamma-aminobutyrate antiporter

Products: L-glutamate [Cytoplasm]; 4-aminobutyrate [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MMNQKKLSLFGFFALTASMVLTVYEYPTFATSKLHLVFFLLLGGLLWFLPVALCAAEMATVEGWKNGGIFSWVSQTLGER
FGFAAIFFQWFQITVGFVTMIYFILGALSYVLNFQALNTDPLIKFIGLLIIFWGLTFSQLGGTQRTAKLVKAGFVVGIVI
PSVILFGLAAAYFIGGNPIEIPINSHAFVPDFSQVSTLVVFVSFILAYMGVEASASHINELENPKRNYPLAMILLVILAI
SLDAIGGFSVAAVIPQKELSLSAGVIQTFQTLILHFNHHLGWLVKVIALMIAFGVMGEVSSWVVGPSRGMFAAAQRGLLP
KFLRKTNTHEVPVPLVMIQGIIVTLWGAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFIVYFVLIYKKQNLKRTYNVP
GKIIGKTIIAGIGFLLSIFALFISFVPPASIAKNETHTYQMILLISFVVTAILPFIIYELHDKKGHDTIEEPTHFKAGDV
NPAIYPAARGEHHIIKKEEHILKH

Sequences:

>Translated_504_residues
MMNQKKLSLFGFFALTASMVLTVYEYPTFATSKLHLVFFLLLGGLLWFLPVALCAAEMATVEGWKNGGIFSWVSQTLGER
FGFAAIFFQWFQITVGFVTMIYFILGALSYVLNFQALNTDPLIKFIGLLIIFWGLTFSQLGGTQRTAKLVKAGFVVGIVI
PSVILFGLAAAYFIGGNPIEIPINSHAFVPDFSQVSTLVVFVSFILAYMGVEASASHINELENPKRNYPLAMILLVILAI
SLDAIGGFSVAAVIPQKELSLSAGVIQTFQTLILHFNHHLGWLVKVIALMIAFGVMGEVSSWVVGPSRGMFAAAQRGLLP
KFLRKTNTHEVPVPLVMIQGIIVTLWGAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFIVYFVLIYKKQNLKRTYNVP
GKIIGKTIIAGIGFLLSIFALFISFVPPASIAKNETHTYQMILLISFVVTAILPFIIYELHDKKGHDTIEEPTHFKAGDV
NPAIYPAARGEHHIIKKEEHILKH
>Mature_504_residues
MMNQKKLSLFGFFALTASMVLTVYEYPTFATSKLHLVFFLLLGGLLWFLPVALCAAEMATVEGWKNGGIFSWVSQTLGER
FGFAAIFFQWFQITVGFVTMIYFILGALSYVLNFQALNTDPLIKFIGLLIIFWGLTFSQLGGTQRTAKLVKAGFVVGIVI
PSVILFGLAAAYFIGGNPIEIPINSHAFVPDFSQVSTLVVFVSFILAYMGVEASASHINELENPKRNYPLAMILLVILAI
SLDAIGGFSVAAVIPQKELSLSAGVIQTFQTLILHFNHHLGWLVKVIALMIAFGVMGEVSSWVVGPSRGMFAAAQRGLLP
KFLRKTNTHEVPVPLVMIQGIIVTLWGAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFIVYFVLIYKKQNLKRTYNVP
GKIIGKTIIAGIGFLLSIFALFISFVPPASIAKNETHTYQMILLISFVVTAILPFIIYELHDKKGHDTIEEPTHFKAGDV
NPAIYPAARGEHHIIKKEEHILKH

Specific function: Involved in glutamate-dependent acid resistance. May be involved in antiport of glutamate and glutamate-gamma- aminobutyrate (GABA) [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Glutamate:GABA antiporter (GGA) (TC 2.A.3.7) family [H]

Homologues:

Organism=Escherichia coli, GI1787768, Length=502, Percent_Identity=52.7888446215139, Blast_Score=527, Evalue=1e-151,
Organism=Escherichia coli, GI87082380, Length=482, Percent_Identity=22.6141078838174, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI48994921, Length=427, Percent_Identity=20.8430913348946, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI2367353, Length=280, Percent_Identity=22.8571428571429, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004759 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 55501; Mature: 55501

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCCCCCCHHHHHCC
>Mature Secondary Structure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CCCCCCCCCCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-aminobutyrate [Cytoplasm]; L-glutamate [Periplasm] [C]

Specific reaction: 4-aminobutyrate [Cytoplasm] + L-glutamate [Periplasm] = L-glutamate [Cytoplasm] + 4-aminobutyrate [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11337471; 10411264 [H]