Click here to switch to the map view.

The map label for this gene is lacF [H]

Identifier: 125623994

GI number: 125623994

Start: 1130161

End: 1131120

Strand: Direct

Name: lacF [H]

Synonym: llmg_1164

Alternate gene names: 125623994

Gene position: 1130161-1131120 (Clockwise)

Preceding gene: 125623993

Following gene: 125623995

Centisome position: 44.68

GC content: 37.29

Gene sequence:

>960_bases
ATGGAAAACACCAAAAAAAAGTCATCCAAGAAAAAACTTTTTGAAAGGTACAACCGTTTTGGTTATTACTTTTTAATTCC
CTTTTTCGTTGGATTTATCGTCTTTCAACTTTATCCAATTATTTACTCGATTGCGATTAGCTTTACGAGTATGAATGGTT
TTCAAACTTTTTCCGAGGCCACAAATGTTGGTTTAGGAAACTTTTCTACACTTTTAAAGAATACTCCTTTGTTTATCACG
AGTATTCAAAACACTTTACTTTTGTGGGGAATGAATTTTGTACCACAAATTTTAATGGCTCTGATTCTTGCTGCTTGGTG
GACTAATCGGAAGCTCAATCTAAAAGGTAATAGCTTTTGGAAAACAGTTTTTTATCTGCCAAATATGATTATGGCAGCTT
CTATTGCGGCGCTCTTTTTGGGGATTTTTGGTTATCCAAATGGTCCAGCCAATCTTTTGCTCACAAATCTGCATATTATC
AGTCATCCATTCAATTTCTTCCAGTCTATTTGGGGGACAAGAAGTATTATCATCTTCTTACAATTTTGGATGTGGTATGG
TTCAACGGCGATAGTACTTGTCGCTGGGATTTCCTCGATTGATGATGCTTTGTTTGAAGCGGCACGGATAGATGGAGCTT
CTGACGGACAGATTTTTAGAAAGATTACCTTACCTTTATTGCGTCCGATTGTCATGTACACATTTGTAACCAGCTTTGTT
GGTGGCTTACAAGTGTTTGATATTCCTTATCTTTTATCTTCTGGTTCAGGTGGTCCACAAAATTCAACTTATACGCTGGC
TATGTTTATCTACAATCAGGCCTTTCAATACCGAAATTATGGAATTGCGGCAGCAGCATCAGTGATTCTGTTGATTATCG
TGGCGATTGTTTCTATTGCTCTTTTCCGTTTCTTCCGTGAACGGACGAGCAATAAGGATTTGGTAGGAGGTAAAGATTGA

Upstream 100 bases:

>100_bases
CGCTTTAAGTTGAAGCTATTACCATTTTTTAATGGAATTAAGTTTCTGATGAGTGAATAACATTATTCAATTTCCCTTTT
ATTTTTTAGAGGAGGGTGAT

Downstream 100 bases:

>100_bases
TGAATAAAAGCTATGCAGGTTCACTAAGAGCCAGACGTACTTTGATTTATATCTGTTTTGGTATTTTGACACTTTTGAGT
CTCTTGCCTTTTGCAATCAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MENTKKKSSKKKLFERYNRFGYYFLIPFFVGFIVFQLYPIIYSIAISFTSMNGFQTFSEATNVGLGNFSTLLKNTPLFIT
SIQNTLLLWGMNFVPQILMALILAAWWTNRKLNLKGNSFWKTVFYLPNMIMAASIAALFLGIFGYPNGPANLLLTNLHII
SHPFNFFQSIWGTRSIIIFLQFWMWYGSTAIVLVAGISSIDDALFEAARIDGASDGQIFRKITLPLLRPIVMYTFVTSFV
GGLQVFDIPYLLSSGSGGPQNSTYTLAMFIYNQAFQYRNYGIAAAASVILLIIVAIVSIALFRFFRERTSNKDLVGGKD

Sequences:

>Translated_319_residues
MENTKKKSSKKKLFERYNRFGYYFLIPFFVGFIVFQLYPIIYSIAISFTSMNGFQTFSEATNVGLGNFSTLLKNTPLFIT
SIQNTLLLWGMNFVPQILMALILAAWWTNRKLNLKGNSFWKTVFYLPNMIMAASIAALFLGIFGYPNGPANLLLTNLHII
SHPFNFFQSIWGTRSIIIFLQFWMWYGSTAIVLVAGISSIDDALFEAARIDGASDGQIFRKITLPLLRPIVMYTFVTSFV
GGLQVFDIPYLLSSGSGGPQNSTYTLAMFIYNQAFQYRNYGIAAAASVILLIIVAIVSIALFRFFRERTSNKDLVGGKD
>Mature_319_residues
MENTKKKSSKKKLFERYNRFGYYFLIPFFVGFIVFQLYPIIYSIAISFTSMNGFQTFSEATNVGLGNFSTLLKNTPLFIT
SIQNTLLLWGMNFVPQILMALILAAWWTNRKLNLKGNSFWKTVFYLPNMIMAASIAALFLGIFGYPNGPANLLLTNLHII
SHPFNFFQSIWGTRSIIIFLQFWMWYGSTAIVLVAGISSIDDALFEAARIDGASDGQIFRKITLPLLRPIVMYTFVTSFV
GGLQVFDIPYLLSSGSGGPQNSTYTLAMFIYNQAFQYRNYGIAAAASVILLIIVAIVSIALFRFFRERTSNKDLVGGKD

Specific function: Part of the binding-protein-dependent transport system for lactose. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789861, Length=307, Percent_Identity=27.3615635179153, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787570, Length=258, Percent_Identity=25.1937984496124, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1790465, Length=204, Percent_Identity=25.9803921568627, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35988; Mature: 35988

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENTKKKSSKKKLFERYNRFGYYFLIPFFVGFIVFQLYPIIYSIAISFTSMNGFQTFSEA
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
TNVGLGNFSTLLKNTPLFITSIQNTLLLWGMNFVPQILMALILAAWWTNRKLNLKGNSFW
HCCCCCHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHH
KTVFYLPNMIMAASIAALFLGIFGYPNGPANLLLTNLHIISHPFNFFQSIWGTRSIIIFL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHH
QFWMWYGSTAIVLVAGISSIDDALFEAARIDGASDGQIFRKITLPLLRPIVMYTFVTSFV
HHHHHHCCHHHHHHHCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GGLQVFDIPYLLSSGSGGPQNSTYTLAMFIYNQAFQYRNYGIAAAASVILLIIVAIVSIA
CCHHHEEHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LFRFFRERTSNKDLVGGKD
HHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MENTKKKSSKKKLFERYNRFGYYFLIPFFVGFIVFQLYPIIYSIAISFTSMNGFQTFSEA
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
TNVGLGNFSTLLKNTPLFITSIQNTLLLWGMNFVPQILMALILAAWWTNRKLNLKGNSFW
HCCCCCHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHH
KTVFYLPNMIMAASIAALFLGIFGYPNGPANLLLTNLHIISHPFNFFQSIWGTRSIIIFL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHH
QFWMWYGSTAIVLVAGISSIDDALFEAARIDGASDGQIFRKITLPLLRPIVMYTFVTSFV
HHHHHHCCHHHHHHHCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GGLQVFDIPYLLSSGSGGPQNSTYTLAMFIYNQAFQYRNYGIAAAASVILLIIVAIVSIA
CCHHHEEHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LFRFFRERTSNKDLVGGKD
HHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1630315 [H]