Click here to switch to the map view.

The map label for this gene is lmrB [H]

Identifier: 125623935

GI number: 125623935

Start: 1064841

End: 1066205

Strand: Direct

Name: lmrB [H]

Synonym: llmg_1104

Alternate gene names: 125623935

Gene position: 1064841-1066205 (Clockwise)

Preceding gene: 125623931

Following gene: 125623936

Centisome position: 42.1

GC content: 37.58

Gene sequence:

>1365_bases
ATGAATAAAAACAACCATTTCCACTTATTAGGGGCAGTGATTGCTACAGGTATTTTGTCGTTTTCTGGTGTTCTGATTGA
AACCGCGATGAATGTGACTTTCCCAACGCTCATTCATGAGTTTGGACTTTCTACATCAAAAATACAATGGGTAACGACGA
TTTATCTTCTTGTGATTGCGATTACGATTCCTCTCTCTTCTTATTTTAATGAGCGCTTTTCGGCAATAAAGCTTTTCTTG
ATTGCCAACCTTTTCTTTTTAGTTGGAGTCTTAACCAACTGTTTCTCAGCGAATTTTGTCATGCTTTTGTTTGGCCGCCT
TTTACAAGGGATCGGGACAGGGATTGGTTTACCTCTGATGTTCCATCTTATATTAACTAAGGCACCTCTTCATAAAAGAG
GAATGATGATGGGAATTGGAACTCTAACAACCTCTATTGCGCCAGCTATTGGCCCAACTTATGGGGGAATTATTGCTCAT
GCGCTTGATTGGCGCTATATCTACATCTTTTTACTTCCTTTAGTTATCATTTCCTTATTTCTTGGTTTAGCTGCCCTTCC
AAGAGAAATTGCAAAAACACCAAAAAAATTGGCCTTTCGTCCAGTTATTGCCTTGTCAATTATGTTCTTTGCCTTTATTT
CGGCTTTGAGTGCAGAACATTTGCTTACTTTCGCACTTTTGTTCGTCATCGGAATGATTGGAGCAGTCATGTTTGTTCGT
TTCAATCGTAAAGAAACGTTAATTGATTTGGGAATCATGAAAAATCGTCGCTTCGTTGCCTTAATTTTTAGTCTTTTAGT
TTATCAAGCTTTATTATTAGGTTTATCATTTATTTTGCCAAGTTTTCTTCAAGTATCAGCAGGATTTAGTTCATCTGTTG
CCGGAATATTTATGTTTCCTGGAGCTTTGATAGGGGCTGTTTTGGCTCCGATTTCTGGTAAAATCTTAGACCAAATTGGG
GCAAGAAAGCCGATTACGACAGGTTTAGTTTTAGCGGCTTTGGGACTTGTTTTGCTTTTTGTATTTTTACCGACCAAGAG
TCTCGCTCTCCTTTTGATTGCGCACATTGTTTTGATGTTAGGTTTGGGAATTTCTTATAGTAATTTGATGACTTGCAGTT
TGGGCACCTTGTCAACGGAGGAGCTTTCTGATGGAAATGCTTTGGTCAATACTTTACAACAATTTATCGGAGCGGTCGCA
ACGGCAGTAGTTGCAACAACCCTTTCACTCTTCCAAGGTCTTAATGGTTTCAAAACTGGAACAAGCCAAGGAACTTCAAT
TATTTTAGCTTTATTTTTCATCTTAATTATTGTCAGTTTGATTGTAAGTTTCCCAAATTTGAAAAAAATAAAAGCAAATC
ATTAA

Upstream 100 bases:

>100_bases
TACAGATAACATAAATTATGTTGAATTATACAGAGGCGAAATTATTATTCTAGAATAATAATTTCGCCTCTTTTTGTTTA
GTTTTAGGAGATTTTATTGC

Downstream 100 bases:

>100_bases
AAAAATCTTTGACGAATGACACTTTTTTCGTTATAATATTTTAAAACCTTTAACTAAGTCCAGAGAGGCTTGCAAGGATA
CGGAAAATTTATCTTGGATT

Product: drug-export protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MNKNNHFHLLGAVIATGILSFSGVLIETAMNVTFPTLIHEFGLSTSKIQWVTTIYLLVIAITIPLSSYFNERFSAIKLFL
IANLFFLVGVLTNCFSANFVMLLFGRLLQGIGTGIGLPLMFHLILTKAPLHKRGMMMGIGTLTTSIAPAIGPTYGGIIAH
ALDWRYIYIFLLPLVIISLFLGLAALPREIAKTPKKLAFRPVIALSIMFFAFISALSAEHLLTFALLFVIGMIGAVMFVR
FNRKETLIDLGIMKNRRFVALIFSLLVYQALLLGLSFILPSFLQVSAGFSSSVAGIFMFPGALIGAVLAPISGKILDQIG
ARKPITTGLVLAALGLVLLFVFLPTKSLALLLIAHIVLMLGLGISYSNLMTCSLGTLSTEELSDGNALVNTLQQFIGAVA
TAVVATTLSLFQGLNGFKTGTSQGTSIILALFFILIIVSLIVSFPNLKKIKANH

Sequences:

>Translated_454_residues
MNKNNHFHLLGAVIATGILSFSGVLIETAMNVTFPTLIHEFGLSTSKIQWVTTIYLLVIAITIPLSSYFNERFSAIKLFL
IANLFFLVGVLTNCFSANFVMLLFGRLLQGIGTGIGLPLMFHLILTKAPLHKRGMMMGIGTLTTSIAPAIGPTYGGIIAH
ALDWRYIYIFLLPLVIISLFLGLAALPREIAKTPKKLAFRPVIALSIMFFAFISALSAEHLLTFALLFVIGMIGAVMFVR
FNRKETLIDLGIMKNRRFVALIFSLLVYQALLLGLSFILPSFLQVSAGFSSSVAGIFMFPGALIGAVLAPISGKILDQIG
ARKPITTGLVLAALGLVLLFVFLPTKSLALLLIAHIVLMLGLGISYSNLMTCSLGTLSTEELSDGNALVNTLQQFIGAVA
TAVVATTLSLFQGLNGFKTGTSQGTSIILALFFILIIVSLIVSFPNLKKIKANH
>Mature_454_residues
MNKNNHFHLLGAVIATGILSFSGVLIETAMNVTFPTLIHEFGLSTSKIQWVTTIYLLVIAITIPLSSYFNERFSAIKLFL
IANLFFLVGVLTNCFSANFVMLLFGRLLQGIGTGIGLPLMFHLILTKAPLHKRGMMMGIGTLTTSIAPAIGPTYGGIIAH
ALDWRYIYIFLLPLVIISLFLGLAALPREIAKTPKKLAFRPVIALSIMFFAFISALSAEHLLTFALLFVIGMIGAVMFVR
FNRKETLIDLGIMKNRRFVALIFSLLVYQALLLGLSFILPSFLQVSAGFSSSVAGIFMFPGALIGAVLAPISGKILDQIG
ARKPITTGLVLAALGLVLLFVFLPTKSLALLLIAHIVLMLGLGISYSNLMTCSLGTLSTEELSDGNALVNTLQQFIGAVA
TAVVATTLSLFQGLNGFKTGTSQGTSIILALFFILIIVSLIVSFPNLKKIKANH

Specific function: Proton-dependent transporter. May mediate the efflux of lincomycin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=404, Percent_Identity=24.7524752475248, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788710, Length=409, Percent_Identity=25.9168704156479, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1790195, Length=405, Percent_Identity=23.4567901234568, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI87081983, Length=395, Percent_Identity=24.5569620253165, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1788392, Length=418, Percent_Identity=25.1196172248804, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48929; Mature: 48929

Theoretical pI: Translated: 10.68; Mature: 10.68

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure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CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]