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The map label for this gene is rluC [H]

Identifier: 125623931

GI number: 125623931

Start: 1060537

End: 1061070

Strand: Direct

Name: rluC [H]

Synonym: llmg_1100

Alternate gene names: 125623931

Gene position: 1060537-1061070 (Clockwise)

Preceding gene: 125623927

Following gene: 125623935

Centisome position: 41.93

GC content: 36.7

Gene sequence:

>534_bases
ATGATTATTTTATTTAATAAACCTTTTGATGTACTGACAAAATTTACCGATAGCGAGGGTCGCAAAACTTTAAAAGATTA
TATAGAAATACCAAATGTTTATGCGGCAGGCAGATTGGATAAAGATAGTGAAGGTCTACTTTTACTGACAGATGATGGAA
AGCTCAATCATTTGCTGACAGACCCCAAATCGAAGTCTTTTAAAACTTATTTAGTTCAAGTTGAGGGAGAATTTTCGATA
GAAGCGGCACAAAAATTGGAAAAAGGTGTCTTACTCAAAGATGGTAAAACGCTACCTGCTAAAGTAAAATTAATTGATGA
ACCTGAGTGGCTTTGGGAACGGACGCCCCCTATTCGAGAGCGAAAAGCAATTCCGACAAGCTGGGTGGAAATTTCAATTA
AAGAAGGAAAAAATAGACAGATTAGAAGAATGACAGCGGCGGTAGGCTTTCCAACACTTCGTTTAATTCGCTGGTCAATT
GGACAATACACGCTTGGAAATCTAAAATCTGGCGACTATAAAATCATTAAATAA

Upstream 100 bases:

>100_bases
TTTTTGGATTAAAACTTCAGTTGTAAGCTATTAATTATAAATTTTGGTAAAATAATTAGAAGAATAGATTGGGAAAGATG
CAAAATTTGCAAGGAAAATT

Downstream 100 bases:

>100_bases
TAAAAAAAGGAATCACTTAAGATTCCTTTTTTAGTTTAAAAACTACGCCCGCCACCAGAGCCATCACTGAAACTATTATT
GTCAGAAGAAGGACGGGGGA

Product: ribosomal large subunit pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MIILFNKPFDVLTKFTDSEGRKTLKDYIEIPNVYAAGRLDKDSEGLLLLTDDGKLNHLLTDPKSKSFKTYLVQVEGEFSI
EAAQKLEKGVLLKDGKTLPAKVKLIDEPEWLWERTPPIRERKAIPTSWVEISIKEGKNRQIRRMTAAVGFPTLRLIRWSI
GQYTLGNLKSGDYKIIK

Sequences:

>Translated_177_residues
MIILFNKPFDVLTKFTDSEGRKTLKDYIEIPNVYAAGRLDKDSEGLLLLTDDGKLNHLLTDPKSKSFKTYLVQVEGEFSI
EAAQKLEKGVLLKDGKTLPAKVKLIDEPEWLWERTPPIRERKAIPTSWVEISIKEGKNRQIRRMTAAVGFPTLRLIRWSI
GQYTLGNLKSGDYKIIK
>Mature_177_residues
MIILFNKPFDVLTKFTDSEGRKTLKDYIEIPNVYAAGRLDKDSEGLLLLTDDGKLNHLLTDPKSKSFKTYLVQVEGEFSI
EAAQKLEKGVLLKDGKTLPAKVKLIDEPEWLWERTPPIRERKAIPTSWVEISIKEGKNRQIRRMTAAVGFPTLRLIRWSI
GQYTLGNLKSGDYKIIK

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=175, Percent_Identity=60, Blast_Score=228, Evalue=2e-61,
Organism=Escherichia coli, GI1788510, Length=178, Percent_Identity=34.8314606741573, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1787524, Length=169, Percent_Identity=33.1360946745562, Blast_Score=74, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 20247; Mature: 20247

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIILFNKPFDVLTKFTDSEGRKTLKDYIEIPNVYAAGRLDKDSEGLLLLTDDGKLNHLLT
CEEEECCCHHHHHHCCCCCCHHHHHHHHCCCCEEECCCCCCCCCCEEEEECCCCCCEEEC
DPKSKSFKTYLVQVEGEFSIEAAQKLEKGVLLKDGKTLPAKVKLIDEPEWLWERTPPIRE
CCCCCCEEEEEEEECCCCCHHHHHHHHCCEEEECCCCCCEEEEEECCCHHHHCCCCCCCC
RKAIPTSWVEISIKEGKNRQIRRMTAAVGFPTLRLIRWSIGQYTLGNLKSGDYKIIK
CCCCCCEEEEEEEECCCCCHHHHHHHHHCCCHHEEEEECCCCEEECCCCCCCEEEEC
>Mature Secondary Structure
MIILFNKPFDVLTKFTDSEGRKTLKDYIEIPNVYAAGRLDKDSEGLLLLTDDGKLNHLLT
CEEEECCCHHHHHHCCCCCCHHHHHHHHCCCCEEECCCCCCCCCCEEEEECCCCCCEEEC
DPKSKSFKTYLVQVEGEFSIEAAQKLEKGVLLKDGKTLPAKVKLIDEPEWLWERTPPIRE
CCCCCCEEEEEEEECCCCCHHHHHHHHCCEEEECCCCCCEEEEEECCCHHHHCCCCCCCC
RKAIPTSWVEISIKEGKNRQIRRMTAAVGFPTLRLIRWSIGQYTLGNLKSGDYKIIK
CCCCCCEEEEEEEECCCCCHHHHHHHHHCCCHHEEEEECCCCEEECCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]