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The map label for this gene is trpA

Identifier: 125623875

GI number: 125623875

Start: 1003463

End: 1004224

Strand: Direct

Name: trpA

Synonym: llmg_1042

Alternate gene names: 125623875

Gene position: 1003463-1004224 (Clockwise)

Preceding gene: 125623874

Following gene: 125623876

Centisome position: 39.67

GC content: 34.51

Gene sequence:

>762_bases
ATGAAAACTTTACAAGCAAAATTGAGCAATAAAAAAAATAATTTTGTTCCCTATATTATGGCAGGTGACCATGAAAGAGG
ATTAGAAGGGCTCAATGAAACCATTCAACTTCTTGAACAAGCCGGGAGTTCTGCCATTGAAATTGGTGTACCCTTCTCAG
ACCCCGTTGCTGACGGACCAGTAATTGAACAAGCTGGCTTACGAGCCTTAGCCAAAAATGTTTCACTATCAAAGATTCTT
GACAGCTTAAAATCAATTGAAACAGAAGTTCCTCTTGTCATTATGACTTATTTTAATCCTGTCTATCAGTTTGGAATTGA
AAATTTTGTTGCAGCTCTTGAGAGTACAGCAGTAAAAGGTCTAATTATTCCTGATTTACCTAAAGAACATGAAGCTTACA
TTAAGCCTTTTATTACTGATAAGGATATTTGCTTAGTACCTTTGGTGTCATTAACCACACCAATTTCTCGTCAGAAAGAA
CTTGTAGTAGATGCCGAAGGATTCATTTATGCCGTTGCGATCAATGGAGTAACCGGAAAAGAAAATGCTTATAGTAATCA
ACTTGACCATCATTTAGAAACATTGTCTAAATTAACTGATATTCCTGTTTTGACAGGATTTGGGATTTCAACTTTAACAG
ATGTTGAACGTTTTAATAAGGTATCAGCAGGAGTAATCGTTGGTTCAAAAATAGTTAGGGATTTACACGAAAACAAAGAA
AGTGATGTTATTAAATTTATTGAAAACGCAATCAATTTTTAA

Upstream 100 bases:

>100_bases
TAGGTTCAGAAAAAACAATGATTGTTTGCTTATCAGGTCGCGGCGATAAAGATGTTGTTCAAGTTAAAGAACGTCTTGAA
GCAGAAAAAGAGGTGAAATA

Downstream 100 bases:

>100_bases
AAGAATTGAAAAATTAATAAAATCGGATATCCTTGAAGTCCACTTTACAAATGGTATACTTTTATTAAATAAAACGGTTA
CAAAAATTGGATAGTGATTA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MKTLQAKLSNKKNNFVPYIMAGDHERGLEGLNETIQLLEQAGSSAIEIGVPFSDPVADGPVIEQAGLRALAKNVSLSKIL
DSLKSIETEVPLVIMTYFNPVYQFGIENFVAALESTAVKGLIIPDLPKEHEAYIKPFITDKDICLVPLVSLTTPISRQKE
LVVDAEGFIYAVAINGVTGKENAYSNQLDHHLETLSKLTDIPVLTGFGISTLTDVERFNKVSAGVIVGSKIVRDLHENKE
SDVIKFIENAINF

Sequences:

>Translated_253_residues
MKTLQAKLSNKKNNFVPYIMAGDHERGLEGLNETIQLLEQAGSSAIEIGVPFSDPVADGPVIEQAGLRALAKNVSLSKIL
DSLKSIETEVPLVIMTYFNPVYQFGIENFVAALESTAVKGLIIPDLPKEHEAYIKPFITDKDICLVPLVSLTTPISRQKE
LVVDAEGFIYAVAINGVTGKENAYSNQLDHHLETLSKLTDIPVLTGFGISTLTDVERFNKVSAGVIVGSKIVRDLHENKE
SDVIKFIENAINF
>Mature_253_residues
MKTLQAKLSNKKNNFVPYIMAGDHERGLEGLNETIQLLEQAGSSAIEIGVPFSDPVADGPVIEQAGLRALAKNVSLSKIL
DSLKSIETEVPLVIMTYFNPVYQFGIENFVAALESTAVKGLIIPDLPKEHEAYIKPFITDKDICLVPLVSLTTPISRQKE
LVVDAEGFIYAVAINGVTGKENAYSNQLDHHLETLSKLTDIPVLTGFGISTLTDVERFNKVSAGVIVGSKIVRDLHENKE
SDVIKFIENAINF

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=219, Percent_Identity=31.5068493150685, Blast_Score=112, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321412, Length=258, Percent_Identity=33.7209302325581, Blast_Score=139, Evalue=5e-34,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_LACLM (A2RK25)

Other databases:

- EMBL:   AM406671
- RefSeq:   YP_001032358.1
- ProteinModelPortal:   A2RK25
- SMR:   A2RK25
- STRING:   A2RK25
- GeneID:   4796674
- GenomeReviews:   AM406671_GR
- KEGG:   llm:llmg_1042
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 27651; Mature: 27651

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 47-47 ACT_SITE 58-58

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLQAKLSNKKNNFVPYIMAGDHERGLEGLNETIQLLEQAGSSAIEIGVPFSDPVADGP
CCCHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
VIEQAGLRALAKNVSLSKILDSLKSIETEVPLVIMTYFNPVYQFGIENFVAALESTAVKG
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCC
LIIPDLPKEHEAYIKPFITDKDICLVPLVSLTTPISRQKELVVDAEGFIYAVAINGVTGK
EECCCCCCHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCEEEEEEEECCCCC
ENAYSNQLDHHLETLSKLTDIPVLTGFGISTLTDVERFNKVSAGVIVGSKIVRDLHENKE
CCHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
SDVIKFIENAINF
HHHHHHHHHHHCC
>Mature Secondary Structure
MKTLQAKLSNKKNNFVPYIMAGDHERGLEGLNETIQLLEQAGSSAIEIGVPFSDPVADGP
CCCHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
VIEQAGLRALAKNVSLSKILDSLKSIETEVPLVIMTYFNPVYQFGIENFVAALESTAVKG
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCC
LIIPDLPKEHEAYIKPFITDKDICLVPLVSLTTPISRQKELVVDAEGFIYAVAINGVTGK
EECCCCCCHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCEEEEEEEECCCCC
ENAYSNQLDHHLETLSKLTDIPVLTGFGISTLTDVERFNKVSAGVIVGSKIVRDLHENKE
CCHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
SDVIKFIENAINF
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA