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The map label for this gene is gmuD [H]

Identifier: 125623841

GI number: 125623841

Start: 969894

End: 970571

Strand: Direct

Name: gmuD [H]

Synonym: llmg_1004

Alternate gene names: 125623841

Gene position: 969894-970571 (Clockwise)

Preceding gene: 125623839

Following gene: 125623842

Centisome position: 38.34

GC content: 33.19

Gene sequence:

>678_bases
TTGATAGTAGATTTAAAACTTTGCAACAGAACCGTCAAAATTCTATCAGAAGAAAATGTTTTATGGTCAGCAGAATCAGC
AGATATAAAATGCATAAAAGAAAATAAAATTGACCTATTAGGAGTCAATTATTATCATCCAAGTCGTCTGCAAGAACCTG
AATATTCCAGTGATAGTCTGGCACAAGATTGGCGTCCGGATAAATATTATGCCCCATATAATAAGCGAGGGGTCCGAATG
AATGCTGACAGGGGATGGGAAATTCATCCTCAAACCATTTATGAAATTGCCAAGCGAATCCAAGAAGATTATGGAAATAT
TCCTTGGTTTATTTCTGAAAATGGGATGGGCGTGGAAAATGAAGAACGTTTTAAAAATGAAGAGGGTCAAATTCAAGATG
ATTATCGAATTACTTTTACGATAGAACATCTTTATTGGCTACATAAGGCAATTCAAGAAGGTTCAAATTGTTTTGGATAT
CATGTGTGGACACCAATTGATTGTTGGAGTTGGCGAAATTCTTATAAGAACCGCTATGGCTTGATTAGCTTAAATGTTCA
TACTCAAGTCAAAACTTTAAAAAAATCTGCTTATTGGTATAAAAATTTAACTGATTCATCACTTTTACAAGTTCCCAATT
CGATTTTAGAAAAGTATGATGAAGTTTTAAAAAATTAA

Upstream 100 bases:

>100_bases
TAGCCCACGGCTACAATAATCACATCCTGCTGAAATTGCTTTCCTTTAAAATGATTCATCGTCATTCCTCCTGCTATCTT
TTTCTATTATTCTACCTTAT

Downstream 100 bases:

>100_bases
GTAGAGGCAAAAATTCATAGAAGAACTTTAGGAGGAAAAAAATGTCATTATTAACAATCGATATTGGTGGAACCAGCATT
AAATATGCAAGATTTGCTGA

Product: beta-glucosidase

Products: NA

Alternate protein names: Aryl-phospho-beta-D-glucosidase BglD; Glucomannan utilization protein D [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MIVDLKLCNRTVKILSEENVLWSAESADIKCIKENKIDLLGVNYYHPSRLQEPEYSSDSLAQDWRPDKYYAPYNKRGVRM
NADRGWEIHPQTIYEIAKRIQEDYGNIPWFISENGMGVENEERFKNEEGQIQDDYRITFTIEHLYWLHKAIQEGSNCFGY
HVWTPIDCWSWRNSYKNRYGLISLNVHTQVKTLKKSAYWYKNLTDSSLLQVPNSILEKYDEVLKN

Sequences:

>Translated_225_residues
MIVDLKLCNRTVKILSEENVLWSAESADIKCIKENKIDLLGVNYYHPSRLQEPEYSSDSLAQDWRPDKYYAPYNKRGVRM
NADRGWEIHPQTIYEIAKRIQEDYGNIPWFISENGMGVENEERFKNEEGQIQDDYRITFTIEHLYWLHKAIQEGSNCFGY
HVWTPIDCWSWRNSYKNRYGLISLNVHTQVKTLKKSAYWYKNLTDSSLLQVPNSILEKYDEVLKN
>Mature_225_residues
MIVDLKLCNRTVKILSEENVLWSAESADIKCIKENKIDLLGVNYYHPSRLQEPEYSSDSLAQDWRPDKYYAPYNKRGVRM
NADRGWEIHPQTIYEIAKRIQEDYGNIPWFISENGMGVENEERFKNEEGQIQDDYRITFTIEHLYWLHKAIQEGSNCFGY
HVWTPIDCWSWRNSYKNRYGLISLNVHTQVKTLKKSAYWYKNLTDSSLLQVPNSILEKYDEVLKN

Specific function: Phospho-beta-D-glucosidase that seems to be involved in the degradation of glucomannan. Is also capable of hydrolyzing aryl-phospho-beta-D-glucosides, although very weakly, and plays only a minor role, if any, in the degradation of these substrates in viv

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Escherichia coli, GI2367174, Length=192, Percent_Identity=31.25, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789070, Length=203, Percent_Identity=28.0788177339901, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI2367270, Length=133, Percent_Identity=33.0827067669173, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17552856, Length=181, Percent_Identity=28.7292817679558, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17539390, Length=181, Percent_Identity=28.7292817679558, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 26744; Mature: 26744

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVDLKLCNRTVKILSEENVLWSAESADIKCIKENKIDLLGVNYYHPSRLQEPEYSSDSL
CEEEHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCH
AQDWRPDKYYAPYNKRGVRMNADRGWEIHPQTIYEIAKRIQEDYGNIPWFISENGMGVEN
HHCCCCCCCCCCCCCCCEEECCCCCCEECHHHHHHHHHHHHHHHCCCCEEECCCCCCCCC
EERFKNEEGQIQDDYRITFTIEHLYWLHKAIQEGSNCFGYHVWTPIDCWSWRNSYKNRYG
HHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCEE
LISLNVHTQVKTLKKSAYWYKNLTDSSLLQVPNSILEKYDEVLKN
EEEEEEHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIVDLKLCNRTVKILSEENVLWSAESADIKCIKENKIDLLGVNYYHPSRLQEPEYSSDSL
CEEEHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCH
AQDWRPDKYYAPYNKRGVRMNADRGWEIHPQTIYEIAKRIQEDYGNIPWFISENGMGVEN
HHCCCCCCCCCCCCCCCEEECCCCCCEECHHHHHHHHHHHHHHHCCCCEEECCCCCCCCC
EERFKNEEGQIQDDYRITFTIEHLYWLHKAIQEGSNCFGYHVWTPIDCWSWRNSYKNRYG
HHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCEE
LISLNVHTQVKTLKKSAYWYKNLTDSSLLQVPNSILEKYDEVLKN
EEEEEEHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]