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The map label for this gene is murD [H]

Identifier: 125623755

GI number: 125623755

Start: 878459

End: 879808

Strand: Direct

Name: murD [H]

Synonym: llmg_0912

Alternate gene names: 125623755

Gene position: 878459-879808 (Clockwise)

Preceding gene: 125623754

Following gene: 125623756

Centisome position: 34.73

GC content: 36.67

Gene sequence:

>1350_bases
ATGAAAAAAATAACGAAATTTAAAGATCAAAAAATCTTAGTTCTTGGACTTGCCCGTTCTGGAATGGCTGCTGCTCTTGT
GCTCAATGAGTTGGGAGCGATTGTTACAGTTAATGATGGCAAACCTTTTGAAGAAAATAAAGAAGCTCAAGTACTATTAG
AAGAAGGGATTAAAGTCATCACAGGAAGTCACCCGATTGATTTGTTGGACGAAGATTTTGCACTGATGGTTAAAAATCCA
GGAATTCGTTATGATAATCCCATGGTTGAGCGAGCAGAAGCTCTAAAAATTCCAGTGATTACTGAGGTTGAACTTGCATA
TTTAGTGAGTGAGGCACCAATTATTGGCATCACAGGAACAAATGGTAAAACGACAACAACGACATTAATTGCTGATATTT
TAAATGCTGATGGTCAATCTGCTAAGTTATCAGGTAATATTGGTTTTCCAGCGTCAGAAGTTGCCCAAAAAGCTCAAGCT
ACTGACACATTAGTGATGGAACTTTCAAGTTTCCAATTGATGGGAATTGATACTTTCAGACCTAAAATTGCTTTAATCAC
AAATCTTTTTTCTGCTCATTTGGACTACCATGGTTCACAAGAAGCATATGAAGCTGCTAAATGGCGGATTCAAGAAAATA
TGACAGCAGATGATTTCCTAATTCTCAATTTTAACCAAGAAAAATGCCGTAAATTAGCAGATAAAACAAAGGCAACGGTC
CTTGCTTTTTCAACTAAAGAAAAAGTTTCAGGTGCTTATGTTAATGAGGGGAAAATTTATTTTAAAGATGAATTTATCAT
GGAAGCTTCTGAGCTTTCACTTCCTGGGGATCATAATTTAGAAAATGCTTTAGCGGCAATTGTCGCTTCAAAATTACGCG
GAGCAGAAAATGAAGCAATTGTCGAAGTACTGACAAGTTTTGCAGGTGTAAAACATCGGTTGCAATATCTAGGTGAAATT
GATGGACGAAAAGTCTATAATGACAGTAAAGCAACTAATATTTTGGCTACTCAAAAAGCCTTGTCAGGATTTGACAACAG
TAAACTTTGGCTTTTAGCTGGTGGACTTGACCGAGGCAATGGCTTTGAAGACTTAGAAAAAGATTTAGAAGACTTAAAAG
GAATGGTTGTCTTTGGACAAACTGCGGATAAATTGCGGTTGATGGCTGAAAAATTGAATATTCCTGTGTTTGCTAGTCAA
AATGTTGCAACTGCTCTTAAAGAAATCATGCCTCAAACGCAAGTTGGTGATACCATTTTACTTAGCCCAGCTTGTGCAAG
TTGGGATCAATATAAAACCTTTGAAGAACGAGGTGATTTATTTATCGAGGCTTTTGAGAGCTTAAAATAA

Upstream 100 bases:

>100_bases
AGTAATTATGACTGTTGAAAAAAAGGCTGATTAGGGGTAAAATAGATTAGATAAAGTTGTCAGTGAAATATTTTACTGAC
AACTTAAAAAGGAATAGAAA

Downstream 100 bases:

>100_bases
AAAATATGATAGAATAAATTACTGACAATTATAATTGGGACTAATTTGAATACTCAATTTACTGACAGATTAATATAAAC
AAGTTCATTTTACTGACAAT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MKKITKFKDQKILVLGLARSGMAAALVLNELGAIVTVNDGKPFEENKEAQVLLEEGIKVITGSHPIDLLDEDFALMVKNP
GIRYDNPMVERAEALKIPVITEVELAYLVSEAPIIGITGTNGKTTTTTLIADILNADGQSAKLSGNIGFPASEVAQKAQA
TDTLVMELSSFQLMGIDTFRPKIALITNLFSAHLDYHGSQEAYEAAKWRIQENMTADDFLILNFNQEKCRKLADKTKATV
LAFSTKEKVSGAYVNEGKIYFKDEFIMEASELSLPGDHNLENALAAIVASKLRGAENEAIVEVLTSFAGVKHRLQYLGEI
DGRKVYNDSKATNILATQKALSGFDNSKLWLLAGGLDRGNGFEDLEKDLEDLKGMVVFGQTADKLRLMAEKLNIPVFASQ
NVATALKEIMPQTQVGDTILLSPACASWDQYKTFEERGDLFIEAFESLK

Sequences:

>Translated_449_residues
MKKITKFKDQKILVLGLARSGMAAALVLNELGAIVTVNDGKPFEENKEAQVLLEEGIKVITGSHPIDLLDEDFALMVKNP
GIRYDNPMVERAEALKIPVITEVELAYLVSEAPIIGITGTNGKTTTTTLIADILNADGQSAKLSGNIGFPASEVAQKAQA
TDTLVMELSSFQLMGIDTFRPKIALITNLFSAHLDYHGSQEAYEAAKWRIQENMTADDFLILNFNQEKCRKLADKTKATV
LAFSTKEKVSGAYVNEGKIYFKDEFIMEASELSLPGDHNLENALAAIVASKLRGAENEAIVEVLTSFAGVKHRLQYLGEI
DGRKVYNDSKATNILATQKALSGFDNSKLWLLAGGLDRGNGFEDLEKDLEDLKGMVVFGQTADKLRLMAEKLNIPVFASQ
NVATALKEIMPQTQVGDTILLSPACASWDQYKTFEERGDLFIEAFESLK
>Mature_449_residues
MKKITKFKDQKILVLGLARSGMAAALVLNELGAIVTVNDGKPFEENKEAQVLLEEGIKVITGSHPIDLLDEDFALMVKNP
GIRYDNPMVERAEALKIPVITEVELAYLVSEAPIIGITGTNGKTTTTTLIADILNADGQSAKLSGNIGFPASEVAQKAQA
TDTLVMELSSFQLMGIDTFRPKIALITNLFSAHLDYHGSQEAYEAAKWRIQENMTADDFLILNFNQEKCRKLADKTKATV
LAFSTKEKVSGAYVNEGKIYFKDEFIMEASELSLPGDHNLENALAAIVASKLRGAENEAIVEVLTSFAGVKHRLQYLGEI
DGRKVYNDSKATNILATQKALSGFDNSKLWLLAGGLDRGNGFEDLEKDLEDLKGMVVFGQTADKLRLMAEKLNIPVFASQ
NVATALKEIMPQTQVGDTILLSPACASWDQYKTFEERGDLFIEAFESLK

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=457, Percent_Identity=32.1663019693654, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1790680, Length=350, Percent_Identity=24.2857142857143, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 49193; Mature: 49193

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKITKFKDQKILVLGLARSGMAAALVLNELGAIVTVNDGKPFEENKEAQVLLEEGIKVI
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHCCCEEE
TGSHPIDLLDEDFALMVKNPGIRYDNPMVERAEALKIPVITEVELAYLVSEAPIIGITGT
ECCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCEEEHHHHHHHHCCCCEEEEECC
NGKTTTTTLIADILNADGQSAKLSGNIGFPASEVAQKAQATDTLVMELSSFQLMGIDTFR
CCCCHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCC
PKIALITNLFSAHLDYHGSQEAYEAAKWRIQENMTADDFLILNFNQEKCRKLADKTKATV
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHEEE
LAFSTKEKVSGAYVNEGKIYFKDEFIMEASELSLPGDHNLENALAAIVASKLRGAENEAI
EEEECHHHCCCEEEECCEEEEEHHHHEEHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHH
VEVLTSFAGVKHRLQYLGEIDGRKVYNDSKATNILATQKALSGFDNSKLWLLAGGLDRGN
HHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCC
GFEDLEKDLEDLKGMVVFGQTADKLRLMAEKLNIPVFASQNVATALKEIMPQTQVGDTIL
CHHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCCCEEECCHHHHHHHHHCCCCCCCCEEE
LSPACASWDQYKTFEERGDLFIEAFESLK
ECCCCCCCHHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure
MKKITKFKDQKILVLGLARSGMAAALVLNELGAIVTVNDGKPFEENKEAQVLLEEGIKVI
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHCCCEEE
TGSHPIDLLDEDFALMVKNPGIRYDNPMVERAEALKIPVITEVELAYLVSEAPIIGITGT
ECCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCEEEHHHHHHHHCCCCEEEEECC
NGKTTTTTLIADILNADGQSAKLSGNIGFPASEVAQKAQATDTLVMELSSFQLMGIDTFR
CCCCHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCC
PKIALITNLFSAHLDYHGSQEAYEAAKWRIQENMTADDFLILNFNQEKCRKLADKTKATV
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHEEE
LAFSTKEKVSGAYVNEGKIYFKDEFIMEASELSLPGDHNLENALAAIVASKLRGAENEAI
EEEECHHHCCCEEEECCEEEEEHHHHEEHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHH
VEVLTSFAGVKHRLQYLGEIDGRKVYNDSKATNILATQKALSGFDNSKLWLLAGGLDRGN
HHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCC
GFEDLEKDLEDLKGMVVFGQTADKLRLMAEKLNIPVFASQNVATALKEIMPQTQVGDTIL
CHHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCCCEEECCHHHHHHHHHCCCCCCCCEEE
LSPACASWDQYKTFEERGDLFIEAFESLK
ECCCCCCCHHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]