Click here to switch to the map view.

The map label for this gene is carA [H]

Identifier: 125623737

GI number: 125623737

Start: 864265

End: 865338

Strand: Direct

Name: carA [H]

Synonym: llmg_0894

Alternate gene names: 125623737

Gene position: 864265-865338 (Clockwise)

Preceding gene: 125623736

Following gene: 125623741

Centisome position: 34.17

GC content: 39.2

Gene sequence:

>1074_bases
ATGAGCAAAAGACTTTTAATTTTAGAAGATGGAACTATTTTTGAAGGAGAAGCCCTCGGGGCAAATTTAGATGTGACGGG
TGAGCTTGTCTTTAATACAGGAATGACCGGTTATCAAGAATCAATTACTGACCAATCTTATAACGGGCAAATTTTGACTT
TCACTTATCCAATCGTAGGAAATTACGGAGTTAACCGTGATGATTACGAGTCTATCCACCCAACTTGTAAAGCCGTTGTC
GTTCATGAAGCAGCACGTCGCCCATCAAACTGGCGGATGCAAATGTCATTTGATGAATTTTTAAAATCAAAAAATATTCC
CGGAATCACTGGTGTAGATACAAGAGCAATCACTAAAATTGTTCGTGAACATGGGACAATGAAAGCTTCACTGGTTCAAG
CAAGAGATGAAGTGGACCATCAAATGAGCCAACTTCAAGCTACTGTATTGCCAACTAATCAGGTTGAAACAAGTAGTACA
GCGACTGCTTATCCATCACCAAATACAGGTCGAAAAGTCGTTGTTGTCGATTTCGGATTGAAGCACAGCATTTTACGTGA
ACTATCAAAACGCGAATGTAATTTGACAGTTGTTCCTTACAATACCAGTGCTAAAGAAATTTTGGAAATGGAACCTGACG
GAGTTATGCTGACTAACGGCCCAGGAGATCCAACAGATGTTCCAGAAGCCATTGAAATGATTAAAGAAGTTCAAGGCAAA
ATTCCCATTTTCGGAATATGCTTAGGTCATCAATTATTTAGTCTTGCCAACGGTGCGACAACTTACAAAATGAAATTTGG
TCACCGTGGCTTTAATCATGCTGTTCGCGAAGTTGCAACAGGACGAATTGATTTTACCTCACAAAATCATGGCTATGCAG
TTTCATCAGAAAATTTACCAGAAGATTTGATGATTACCCATGTTGAAATCAATGATAACTCAGTTGAAGGAGTGCGTCAC
AAATATTTCCCTGCATTTTCAGTTCAATTTCACCCAGATGCAGCTCCCGGCCCACATGATGCAAGTTATCTTTTTGACGA
TTTCATGGACCTCATGGATAACTTTAAAAAATAA

Upstream 100 bases:

>100_bases
AAAAGCGAAGTAAGTTGAAATTGATGACAATAGGACAAAAAGAAATATTCAAAACTAAATTGTTCTGTTTTTAAAGCAAA
GTTTAAAGGAAATAATTATT

Downstream 100 bases:

>100_bases
AGTGTTGATATTGTTGGCTTTTGATAGTCCAACTTTCATTAAAATGATTAAAAATAGCAAAAAAAGAGCCTGTTTTATAG
CAGGCTCTTTTTTATAAATT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MSKRLLILEDGTIFEGEALGANLDVTGELVFNTGMTGYQESITDQSYNGQILTFTYPIVGNYGVNRDDYESIHPTCKAVV
VHEAARRPSNWRMQMSFDEFLKSKNIPGITGVDTRAITKIVREHGTMKASLVQARDEVDHQMSQLQATVLPTNQVETSST
ATAYPSPNTGRKVVVVDFGLKHSILRELSKRECNLTVVPYNTSAKEILEMEPDGVMLTNGPGDPTDVPEAIEMIKEVQGK
IPIFGICLGHQLFSLANGATTYKMKFGHRGFNHAVREVATGRIDFTSQNHGYAVSSENLPEDLMITHVEINDNSVEGVRH
KYFPAFSVQFHPDAAPGPHDASYLFDDFMDLMDNFKK

Sequences:

>Translated_357_residues
MSKRLLILEDGTIFEGEALGANLDVTGELVFNTGMTGYQESITDQSYNGQILTFTYPIVGNYGVNRDDYESIHPTCKAVV
VHEAARRPSNWRMQMSFDEFLKSKNIPGITGVDTRAITKIVREHGTMKASLVQARDEVDHQMSQLQATVLPTNQVETSST
ATAYPSPNTGRKVVVVDFGLKHSILRELSKRECNLTVVPYNTSAKEILEMEPDGVMLTNGPGDPTDVPEAIEMIKEVQGK
IPIFGICLGHQLFSLANGATTYKMKFGHRGFNHAVREVATGRIDFTSQNHGYAVSSENLPEDLMITHVEINDNSVEGVRH
KYFPAFSVQFHPDAAPGPHDASYLFDDFMDLMDNFKK
>Mature_356_residues
SKRLLILEDGTIFEGEALGANLDVTGELVFNTGMTGYQESITDQSYNGQILTFTYPIVGNYGVNRDDYESIHPTCKAVVV
HEAARRPSNWRMQMSFDEFLKSKNIPGITGVDTRAITKIVREHGTMKASLVQARDEVDHQMSQLQATVLPTNQVETSSTA
TAYPSPNTGRKVVVVDFGLKHSILRELSKRECNLTVVPYNTSAKEILEMEPDGVMLTNGPGDPTDVPEAIEMIKEVQGKI
PIFGICLGHQLFSLANGATTYKMKFGHRGFNHAVREVATGRIDFTSQNHGYAVSSENLPEDLMITHVEINDNSVEGVRHK
YFPAFSVQFHPDAAPGPHDASYLFDDFMDLMDNFKK

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=359, Percent_Identity=40.6685236768802, Blast_Score=273, Evalue=1e-73,
Organism=Homo sapiens, GI169790915, Length=367, Percent_Identity=37.0572207084469, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI21361331, Length=367, Percent_Identity=37.0572207084469, Blast_Score=226, Evalue=3e-59,
Organism=Escherichia coli, GI1786215, Length=375, Percent_Identity=43.2, Blast_Score=307, Evalue=6e-85,
Organism=Caenorhabditis elegans, GI193204318, Length=359, Percent_Identity=41.5041782729805, Blast_Score=259, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6324878, Length=371, Percent_Identity=39.3530997304582, Blast_Score=261, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6322331, Length=396, Percent_Identity=37.3737373737374, Blast_Score=248, Evalue=8e-67,
Organism=Saccharomyces cerevisiae, GI6322638, Length=137, Percent_Identity=32.1167883211679, Blast_Score=66, Evalue=7e-12,
Organism=Drosophila melanogaster, GI45555749, Length=369, Percent_Identity=39.8373983739837, Blast_Score=260, Evalue=8e-70,
Organism=Drosophila melanogaster, GI24642586, Length=369, Percent_Identity=39.8373983739837, Blast_Score=260, Evalue=1e-69,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 39637; Mature: 39506

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRLLILEDGTIFEGEALGANLDVTGELVFNTGMTGYQESITDQSYNGQILTFTYPIVG
CCCEEEEEECCCEEECCCCCCCCCEEEEEEEECCCCCHHHHHCCCCCCCEEEEEEECEEC
NYGVNRDDYESIHPTCKAVVVHEAARRPSNWRMQMSFDEFLKSKNIPGITGVDTRAITKI
CCCCCCCCHHHHCCHHHHHHHHHHHCCCCCCEEEECHHHHHHHCCCCCCCCCCHHHHHHH
VREHGTMKASLVQARDEVDHQMSQLQATVLPTNQVETSSTATAYPSPNTGRKVVVVDFGL
HHHCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCEECCCCCCCCCEEEEEECCC
KHSILRELSKRECNLTVVPYNTSAKEILEMEPDGVMLTNGPGDPTDVPEAIEMIKEVQGK
HHHHHHHHHHCCCCEEEEECCCCHHHHHEECCCCEEEECCCCCCCCHHHHHHHHHHHHCC
IPIFGICLGHQLFSLANGATTYKMKFGHRGFNHAVREVATGRIDFTSQNHGYAVSSENLP
CCEEEEEHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCEEECCCCCC
EDLMITHVEINDNSVEGVRHKYFPAFSVQFHPDAAPGPHDASYLFDDFMDLMDNFKK
CCCEEEEEEECCCCCCCHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKRLLILEDGTIFEGEALGANLDVTGELVFNTGMTGYQESITDQSYNGQILTFTYPIVG
CCEEEEEECCCEEECCCCCCCCCEEEEEEEECCCCCHHHHHCCCCCCCEEEEEEECEEC
NYGVNRDDYESIHPTCKAVVVHEAARRPSNWRMQMSFDEFLKSKNIPGITGVDTRAITKI
CCCCCCCCHHHHCCHHHHHHHHHHHCCCCCCEEEECHHHHHHHCCCCCCCCCCHHHHHHH
VREHGTMKASLVQARDEVDHQMSQLQATVLPTNQVETSSTATAYPSPNTGRKVVVVDFGL
HHHCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCEECCCCCCCCCEEEEEECCC
KHSILRELSKRECNLTVVPYNTSAKEILEMEPDGVMLTNGPGDPTDVPEAIEMIKEVQGK
HHHHHHHHHHCCCCEEEEECCCCHHHHHEECCCCEEEECCCCCCCCHHHHHHHHHHHHCC
IPIFGICLGHQLFSLANGATTYKMKFGHRGFNHAVREVATGRIDFTSQNHGYAVSSENLP
CCEEEEEHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCEEECCCCCC
EDLMITHVEINDNSVEGVRHKYFPAFSVQFHPDAAPGPHDASYLFDDFMDLMDNFKK
CCCEEEEEEECCCCCCCHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]